Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection

Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Magiera, Sylwia [verfasserIn] Adolf, Weronika Baranowska, Irena

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Chiral separation HPLC Method validation Carvedilol enantiomers 5′-Hyroxyphenyl carvedilol enantiomers

Anmerkung:	© Versita Warsaw and Springer-Verlag Wien 2013

Übergeordnetes Werk:	Enthalten in: Central European journal of chemistry - Warsaw : Central European Science Journals, 2003, 11(2013), 12 vom: 05. Sept., Seite 2076-2087
Übergeordnetes Werk:	volume:11 ; year:2013 ; number:12 ; day:05 ; month:09 ; pages:2076-2087

Links:	Volltext

DOI / URN:	10.2478/s11532-013-0329-x

Katalog-ID:	SPR020938799

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allfields	10.2478/s11532-013-0329-x doi (DE-627)SPR020938799 (SPR)s11532-013-0329-x-e DE-627 ger DE-627 rakwb eng Magiera, Sylwia verfasserin aut Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. Chiral separation (dpeaa)DE-He213 HPLC (dpeaa)DE-He213 Method validation (dpeaa)DE-He213 Carvedilol enantiomers (dpeaa)DE-He213 5′-Hyroxyphenyl carvedilol enantiomers (dpeaa)DE-He213 Adolf, Weronika aut Baranowska, Irena aut Enthalten in Central European journal of chemistry Warsaw : Central European Science Journals, 2003 11(2013), 12 vom: 05. Sept., Seite 2076-2087 (DE-627)358627516 (DE-600)2097193-X 1644-3624 nnns volume:11 year:2013 number:12 day:05 month:09 pages:2076-2087 https://dx.doi.org/10.2478/s11532-013-0329-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2013 12 05 09 2076-2087
spelling	10.2478/s11532-013-0329-x doi (DE-627)SPR020938799 (SPR)s11532-013-0329-x-e DE-627 ger DE-627 rakwb eng Magiera, Sylwia verfasserin aut Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. Chiral separation (dpeaa)DE-He213 HPLC (dpeaa)DE-He213 Method validation (dpeaa)DE-He213 Carvedilol enantiomers (dpeaa)DE-He213 5′-Hyroxyphenyl carvedilol enantiomers (dpeaa)DE-He213 Adolf, Weronika aut Baranowska, Irena aut Enthalten in Central European journal of chemistry Warsaw : Central European Science Journals, 2003 11(2013), 12 vom: 05. Sept., Seite 2076-2087 (DE-627)358627516 (DE-600)2097193-X 1644-3624 nnns volume:11 year:2013 number:12 day:05 month:09 pages:2076-2087 https://dx.doi.org/10.2478/s11532-013-0329-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2013 12 05 09 2076-2087
allfields_unstemmed	10.2478/s11532-013-0329-x doi (DE-627)SPR020938799 (SPR)s11532-013-0329-x-e DE-627 ger DE-627 rakwb eng Magiera, Sylwia verfasserin aut Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. Chiral separation (dpeaa)DE-He213 HPLC (dpeaa)DE-He213 Method validation (dpeaa)DE-He213 Carvedilol enantiomers (dpeaa)DE-He213 5′-Hyroxyphenyl carvedilol enantiomers (dpeaa)DE-He213 Adolf, Weronika aut Baranowska, Irena aut Enthalten in Central European journal of chemistry Warsaw : Central European Science Journals, 2003 11(2013), 12 vom: 05. Sept., Seite 2076-2087 (DE-627)358627516 (DE-600)2097193-X 1644-3624 nnns volume:11 year:2013 number:12 day:05 month:09 pages:2076-2087 https://dx.doi.org/10.2478/s11532-013-0329-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2013 12 05 09 2076-2087
allfieldsGer	10.2478/s11532-013-0329-x doi (DE-627)SPR020938799 (SPR)s11532-013-0329-x-e DE-627 ger DE-627 rakwb eng Magiera, Sylwia verfasserin aut Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. Chiral separation (dpeaa)DE-He213 HPLC (dpeaa)DE-He213 Method validation (dpeaa)DE-He213 Carvedilol enantiomers (dpeaa)DE-He213 5′-Hyroxyphenyl carvedilol enantiomers (dpeaa)DE-He213 Adolf, Weronika aut Baranowska, Irena aut Enthalten in Central European journal of chemistry Warsaw : Central European Science Journals, 2003 11(2013), 12 vom: 05. Sept., Seite 2076-2087 (DE-627)358627516 (DE-600)2097193-X 1644-3624 nnns volume:11 year:2013 number:12 day:05 month:09 pages:2076-2087 https://dx.doi.org/10.2478/s11532-013-0329-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2013 12 05 09 2076-2087
allfieldsSound	10.2478/s11532-013-0329-x doi (DE-627)SPR020938799 (SPR)s11532-013-0329-x-e DE-627 ger DE-627 rakwb eng Magiera, Sylwia verfasserin aut Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Versita Warsaw and Springer-Verlag Wien 2013 Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. Chiral separation (dpeaa)DE-He213 HPLC (dpeaa)DE-He213 Method validation (dpeaa)DE-He213 Carvedilol enantiomers (dpeaa)DE-He213 5′-Hyroxyphenyl carvedilol enantiomers (dpeaa)DE-He213 Adolf, Weronika aut Baranowska, Irena aut Enthalten in Central European journal of chemistry Warsaw : Central European Science Journals, 2003 11(2013), 12 vom: 05. Sept., Seite 2076-2087 (DE-627)358627516 (DE-600)2097193-X 1644-3624 nnns volume:11 year:2013 number:12 day:05 month:09 pages:2076-2087 https://dx.doi.org/10.2478/s11532-013-0329-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2013 12 05 09 2076-2087
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source	Enthalten in Central European journal of chemistry 11(2013), 12 vom: 05. Sept., Seite 2076-2087 volume:11 year:2013 number:12 day:05 month:09 pages:2076-2087
sourceStr	Enthalten in Central European journal of chemistry 11(2013), 12 vom: 05. Sept., Seite 2076-2087 volume:11 year:2013 number:12 day:05 month:09 pages:2076-2087
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topic_facet	Chiral separation HPLC Method validation Carvedilol enantiomers 5′-Hyroxyphenyl carvedilol enantiomers
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author	Magiera, Sylwia
spellingShingle	Magiera, Sylwia misc Chiral separation misc HPLC misc Method validation misc Carvedilol enantiomers misc 5′-Hyroxyphenyl carvedilol enantiomers Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection
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topic_title	Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection Chiral separation (dpeaa)DE-He213 HPLC (dpeaa)DE-He213 Method validation (dpeaa)DE-He213 Carvedilol enantiomers (dpeaa)DE-He213 5′-Hyroxyphenyl carvedilol enantiomers (dpeaa)DE-He213
topic	misc Chiral separation misc HPLC misc Method validation misc Carvedilol enantiomers misc 5′-Hyroxyphenyl carvedilol enantiomers
topic_unstemmed	misc Chiral separation misc HPLC misc Method validation misc Carvedilol enantiomers misc 5′-Hyroxyphenyl carvedilol enantiomers
topic_browse	misc Chiral separation misc HPLC misc Method validation misc Carvedilol enantiomers misc 5′-Hyroxyphenyl carvedilol enantiomers
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title	Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection
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title_full	Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection
author_sort	Magiera, Sylwia
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author_browse	Magiera, Sylwia Adolf, Weronika Baranowska, Irena
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doi_str_mv	10.2478/s11532-013-0329-x
title_sort	simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection
title_auth	Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection
abstract	Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. © Versita Warsaw and Springer-Verlag Wien 2013
abstractGer	Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. © Versita Warsaw and Springer-Verlag Wien 2013
abstract_unstemmed	Abstract A sensitive and specific high performance liquid chromatography coupled with fluorescent detection (HPLC-FL) and tandem mass spectrometry detection (HPLC-MS/MS) methods for separation and determination of carvedilol (CAR) enantiomers and 5′-hydroxyphenyl carvedilol (5′-HCAR) enantiomers has been developed and validated. The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. This newly developed method was successfully used to analyse the aforementioned analytes in human urine samples obtained from patients suffering from cardiovascular disease. © Versita Warsaw and Springer-Verlag Wien 2013
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title_short	Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection
url	https://dx.doi.org/10.2478/s11532-013-0329-x
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up_date	2024-07-03T19:14:00.458Z
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The analysed compounds were extracted from human urine by solid phase extraction. Good enantioseparation of the studied enantiomers was achieved on CHIRALCEL® OD-RH column using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile in a gradient elution. The mass spectrometric data were acquired using the multiple reaction monitoring mode by positive electrospray ionisation. The method was validated over the concentration range from 25.0 ng $ mL^{−1} $ to 200 ng $ mL^{−1} $ for the analysed compounds. The limit of quantification varied from 14.2 ng $ mL^{−1} $ to 24.2 ng $ mL^{−1} $. Both the repeatability and inter-day precisions were below 10.0%, and the accuracy varied from −13.2% to 3.77%. The extraction recoveries ranged from 79.2% to 108%. The present paper reports the method for the simultaneous determination of CAR enantiomers and their metabolite enantiomers (5′-HCAR) in human urine samples. 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Simultaneous chiral separation and determination of carvedilol and 5′-hydroxyphenyl carvedilol enantiomers from human urine by high performance liquid chromatography coupled with fluorescent detection

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