Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells
Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metaboli...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Huang, Jin [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Anmerkung: |
© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of Huazhong University of Science and Technology - Wuhan : Huazhong Univ. of Science and Technology, 2002, 36(2016), 3 vom: Juni, Seite 356-363 |
|---|---|
| Übergeordnetes Werk: |
volume:36 ; year:2016 ; number:3 ; month:06 ; pages:356-363 |
| Links: |
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| DOI / URN: |
10.1007/s11596-016-1591-x |
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| Katalog-ID: |
SPR021009201 |
|---|
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| 100 | 1 | |a Huang, Jin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells |
| 264 | 1 | |c 2016 | |
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| 500 | |a © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 | ||
| 520 | |a Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. | ||
| 700 | 1 | |a Zhou, Chi |4 aut | |
| 700 | 1 | |a He, Jun |4 aut | |
| 700 | 1 | |a Hu, Zheng |4 aut | |
| 700 | 1 | |a Guan, Wen-chao |4 aut | |
| 700 | 1 | |a Liu, Sheng-hong |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of Huazhong University of Science and Technology |d Wuhan : Huazhong Univ. of Science and Technology, 2002 |g 36(2016), 3 vom: Juni, Seite 356-363 |w (DE-627)537444408 |w (DE-600)2376511-2 |x 1993-1352 |7 nnns |
| 773 | 1 | 8 | |g volume:36 |g year:2016 |g number:3 |g month:06 |g pages:356-363 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s11596-016-1591-x |z lizenzpflichtig |3 Volltext |
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| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
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| 912 | |a GBV_ILN_2036 | ||
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| 912 | |a GBV_ILN_2049 | ||
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| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2056 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
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| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
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10.1007/s11596-016-1591-x doi (DE-627)SPR021009201 (SPR)s11596-016-1591-x-e DE-627 ger DE-627 rakwb eng Huang, Jin verfasserin aut Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. Zhou, Chi aut He, Jun aut Hu, Zheng aut Guan, Wen-chao aut Liu, Sheng-hong aut Enthalten in Journal of Huazhong University of Science and Technology Wuhan : Huazhong Univ. of Science and Technology, 2002 36(2016), 3 vom: Juni, Seite 356-363 (DE-627)537444408 (DE-600)2376511-2 1993-1352 nnns volume:36 year:2016 number:3 month:06 pages:356-363 https://dx.doi.org/10.1007/s11596-016-1591-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 36 2016 3 06 356-363 |
| spelling |
10.1007/s11596-016-1591-x doi (DE-627)SPR021009201 (SPR)s11596-016-1591-x-e DE-627 ger DE-627 rakwb eng Huang, Jin verfasserin aut Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. Zhou, Chi aut He, Jun aut Hu, Zheng aut Guan, Wen-chao aut Liu, Sheng-hong aut Enthalten in Journal of Huazhong University of Science and Technology Wuhan : Huazhong Univ. of Science and Technology, 2002 36(2016), 3 vom: Juni, Seite 356-363 (DE-627)537444408 (DE-600)2376511-2 1993-1352 nnns volume:36 year:2016 number:3 month:06 pages:356-363 https://dx.doi.org/10.1007/s11596-016-1591-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 36 2016 3 06 356-363 |
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10.1007/s11596-016-1591-x doi (DE-627)SPR021009201 (SPR)s11596-016-1591-x-e DE-627 ger DE-627 rakwb eng Huang, Jin verfasserin aut Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. Zhou, Chi aut He, Jun aut Hu, Zheng aut Guan, Wen-chao aut Liu, Sheng-hong aut Enthalten in Journal of Huazhong University of Science and Technology Wuhan : Huazhong Univ. of Science and Technology, 2002 36(2016), 3 vom: Juni, Seite 356-363 (DE-627)537444408 (DE-600)2376511-2 1993-1352 nnns volume:36 year:2016 number:3 month:06 pages:356-363 https://dx.doi.org/10.1007/s11596-016-1591-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 36 2016 3 06 356-363 |
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10.1007/s11596-016-1591-x doi (DE-627)SPR021009201 (SPR)s11596-016-1591-x-e DE-627 ger DE-627 rakwb eng Huang, Jin verfasserin aut Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. Zhou, Chi aut He, Jun aut Hu, Zheng aut Guan, Wen-chao aut Liu, Sheng-hong aut Enthalten in Journal of Huazhong University of Science and Technology Wuhan : Huazhong Univ. of Science and Technology, 2002 36(2016), 3 vom: Juni, Seite 356-363 (DE-627)537444408 (DE-600)2376511-2 1993-1352 nnns volume:36 year:2016 number:3 month:06 pages:356-363 https://dx.doi.org/10.1007/s11596-016-1591-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 36 2016 3 06 356-363 |
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10.1007/s11596-016-1591-x doi (DE-627)SPR021009201 (SPR)s11596-016-1591-x-e DE-627 ger DE-627 rakwb eng Huang, Jin verfasserin aut Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. Zhou, Chi aut He, Jun aut Hu, Zheng aut Guan, Wen-chao aut Liu, Sheng-hong aut Enthalten in Journal of Huazhong University of Science and Technology Wuhan : Huazhong Univ. of Science and Technology, 2002 36(2016), 3 vom: Juni, Seite 356-363 (DE-627)537444408 (DE-600)2376511-2 1993-1352 nnns volume:36 year:2016 number:3 month:06 pages:356-363 https://dx.doi.org/10.1007/s11596-016-1591-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 36 2016 3 06 356-363 |
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Enthalten in Journal of Huazhong University of Science and Technology 36(2016), 3 vom: Juni, Seite 356-363 volume:36 year:2016 number:3 month:06 pages:356-363 |
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Enthalten in Journal of Huazhong University of Science and Technology 36(2016), 3 vom: Juni, Seite 356-363 volume:36 year:2016 number:3 month:06 pages:356-363 |
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Huang, Jin @@aut@@ Zhou, Chi @@aut@@ He, Jun @@aut@@ Hu, Zheng @@aut@@ Guan, Wen-chao @@aut@@ Liu, Sheng-hong @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR021009201</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519104811.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201006s2016 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s11596-016-1591-x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR021009201</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s11596-016-1591-x-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Huang, Jin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhou, Chi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Jun</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hu, Zheng</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Guan, Wen-chao</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Sheng-hong</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of 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Huang, Jin |
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Huang, Jin Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells |
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Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells |
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Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells |
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Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells |
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Huang, Jin Zhou, Chi He, Jun Hu, Zheng Guan, Wen-chao Liu, Sheng-hong |
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protective effect of reduced glutathione $ c_{60} $ derivative against hydrogen peroxide-induced apoptosis in hek 293t cells |
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Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells |
| abstract |
Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 |
| abstractGer |
Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 |
| abstract_unstemmed |
Summary Hydrogen peroxide ($ H_{2} %$ O_{2} $) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene ($ C_{60} $) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative ($ C_{60} $-GSH) was successfully synthesized, and its beneficial roles in protecting against $ H_{2} %$ O_{2} $-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated. Fourier Transform infrared spectroscopy and 1H nuclear magnetic resonance were used to confirm the chemical structure of $ C_{60} $-GSH. Our results demonstrated that $ C_{60} $-GSH prevented the reactive oxygen species (ROS)-mediated cell damage. Additionally, $ C_{60} $-GSH pretreatment significantly attenuated $ H_{2} %$ O_{2} $-induced superoxide dismutase (SOD) consumption and malondialdehyde (MDA) elevation. Furthermore, $ C_{60} $-GSH inhibited intracellular calcium mobilization, and subsequent cell apoptosis via bcl-2/bax-caspase-3 signaling pathway induced by $ H_{2} %$ O_{2} $ stimulation in HEK 293T cells. Importantly, these protective effects of $ C_{60} $-GSH were superior to those of GSH. In conclusion, these results suggested that $ C_{60} $-GSH has potential to protect against $ H_{2} %$ O_{2} $-induced cell apoptosis by scavenging free radicals and maintaining intracellular calcium homeostasis without evident toxicity. © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016 |
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| title_short |
Protective effect of reduced glutathione $ C_{60} $ derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells |
| url |
https://dx.doi.org/10.1007/s11596-016-1591-x |
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| author2 |
Zhou, Chi He, Jun Hu, Zheng Guan, Wen-chao Liu, Sheng-hong |
| author2Str |
Zhou, Chi He, Jun Hu, Zheng Guan, Wen-chao Liu, Sheng-hong |
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| doi_str |
10.1007/s11596-016-1591-x |
| up_date |
2024-07-03T19:43:09.534Z |
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| score |
7.3996277 |