Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells
Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of t...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Sivakumar, Allur Subramaniyan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Anmerkung: |
© The Society for In Vitro Biology 2014 |
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| Übergeordnetes Werk: |
Enthalten in: In vitro cellular & developmental biology - Berlin : Springer, 1991, 50(2014), 7 vom: 14. Mai, Seite 630-639 |
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| Übergeordnetes Werk: |
volume:50 ; year:2014 ; number:7 ; day:14 ; month:05 ; pages:630-639 |
| Links: |
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| DOI / URN: |
10.1007/s11626-014-9745-2 |
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| Katalog-ID: |
SPR021215561 |
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| 100 | 1 | |a Sivakumar, Allur Subramaniyan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells |
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| 520 | |a Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. | ||
| 650 | 4 | |a Apoptosis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Inflammatory |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a NF-kB |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MPG |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a T.peptide |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Ochirbat, Chinzorio |4 aut | |
| 700 | 1 | |a Cho, Soo-Hyun |4 aut | |
| 700 | 1 | |a Yang, Jieun |4 aut | |
| 700 | 1 | |a Hwang, Inho |4 aut | |
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| 912 | |a GBV_ILN_2039 | ||
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| 912 | |a GBV_ILN_2049 | ||
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| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2061 | ||
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| 912 | |a GBV_ILN_2068 | ||
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10.1007/s11626-014-9745-2 doi (DE-627)SPR021215561 (SPR)s11626-014-9745-2-e DE-627 ger DE-627 rakwb eng Sivakumar, Allur Subramaniyan verfasserin aut Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2014 Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. Apoptosis (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 NF-kB (dpeaa)DE-He213 MPG (dpeaa)DE-He213 T.peptide (dpeaa)DE-He213 Ochirbat, Chinzorio aut Cho, Soo-Hyun aut Yang, Jieun aut Hwang, Inho aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 50(2014), 7 vom: 14. Mai, Seite 630-639 (DE-627)345200888 (DE-600)2074849-8 1543-706X nnns volume:50 year:2014 number:7 day:14 month:05 pages:630-639 https://dx.doi.org/10.1007/s11626-014-9745-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2144 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 50 2014 7 14 05 630-639 |
| spelling |
10.1007/s11626-014-9745-2 doi (DE-627)SPR021215561 (SPR)s11626-014-9745-2-e DE-627 ger DE-627 rakwb eng Sivakumar, Allur Subramaniyan verfasserin aut Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2014 Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. Apoptosis (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 NF-kB (dpeaa)DE-He213 MPG (dpeaa)DE-He213 T.peptide (dpeaa)DE-He213 Ochirbat, Chinzorio aut Cho, Soo-Hyun aut Yang, Jieun aut Hwang, Inho aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 50(2014), 7 vom: 14. Mai, Seite 630-639 (DE-627)345200888 (DE-600)2074849-8 1543-706X nnns volume:50 year:2014 number:7 day:14 month:05 pages:630-639 https://dx.doi.org/10.1007/s11626-014-9745-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2144 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 50 2014 7 14 05 630-639 |
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10.1007/s11626-014-9745-2 doi (DE-627)SPR021215561 (SPR)s11626-014-9745-2-e DE-627 ger DE-627 rakwb eng Sivakumar, Allur Subramaniyan verfasserin aut Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2014 Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. Apoptosis (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 NF-kB (dpeaa)DE-He213 MPG (dpeaa)DE-He213 T.peptide (dpeaa)DE-He213 Ochirbat, Chinzorio aut Cho, Soo-Hyun aut Yang, Jieun aut Hwang, Inho aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 50(2014), 7 vom: 14. Mai, Seite 630-639 (DE-627)345200888 (DE-600)2074849-8 1543-706X nnns volume:50 year:2014 number:7 day:14 month:05 pages:630-639 https://dx.doi.org/10.1007/s11626-014-9745-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2144 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 50 2014 7 14 05 630-639 |
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10.1007/s11626-014-9745-2 doi (DE-627)SPR021215561 (SPR)s11626-014-9745-2-e DE-627 ger DE-627 rakwb eng Sivakumar, Allur Subramaniyan verfasserin aut Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2014 Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. Apoptosis (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 NF-kB (dpeaa)DE-He213 MPG (dpeaa)DE-He213 T.peptide (dpeaa)DE-He213 Ochirbat, Chinzorio aut Cho, Soo-Hyun aut Yang, Jieun aut Hwang, Inho aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 50(2014), 7 vom: 14. Mai, Seite 630-639 (DE-627)345200888 (DE-600)2074849-8 1543-706X nnns volume:50 year:2014 number:7 day:14 month:05 pages:630-639 https://dx.doi.org/10.1007/s11626-014-9745-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2144 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 50 2014 7 14 05 630-639 |
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10.1007/s11626-014-9745-2 doi (DE-627)SPR021215561 (SPR)s11626-014-9745-2-e DE-627 ger DE-627 rakwb eng Sivakumar, Allur Subramaniyan verfasserin aut Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2014 Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. Apoptosis (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 NF-kB (dpeaa)DE-He213 MPG (dpeaa)DE-He213 T.peptide (dpeaa)DE-He213 Ochirbat, Chinzorio aut Cho, Soo-Hyun aut Yang, Jieun aut Hwang, Inho aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 50(2014), 7 vom: 14. Mai, Seite 630-639 (DE-627)345200888 (DE-600)2074849-8 1543-706X nnns volume:50 year:2014 number:7 day:14 month:05 pages:630-639 https://dx.doi.org/10.1007/s11626-014-9745-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2144 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 50 2014 7 14 05 630-639 |
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Enthalten in In vitro cellular & developmental biology 50(2014), 7 vom: 14. Mai, Seite 630-639 volume:50 year:2014 number:7 day:14 month:05 pages:630-639 |
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Enthalten in In vitro cellular & developmental biology 50(2014), 7 vom: 14. Mai, Seite 630-639 volume:50 year:2014 number:7 day:14 month:05 pages:630-639 |
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Apoptosis Inflammatory NF-kB MPG T.peptide |
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Sivakumar, Allur Subramaniyan @@aut@@ Ochirbat, Chinzorio @@aut@@ Cho, Soo-Hyun @@aut@@ Yang, Jieun @@aut@@ Hwang, Inho @@aut@@ |
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2014-05-14T00:00:00Z |
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| author |
Sivakumar, Allur Subramaniyan |
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Sivakumar, Allur Subramaniyan misc Apoptosis misc Inflammatory misc NF-kB misc MPG misc T.peptide Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells |
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Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells Apoptosis (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 NF-kB (dpeaa)DE-He213 MPG (dpeaa)DE-He213 T.peptide (dpeaa)DE-He213 |
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misc Apoptosis misc Inflammatory misc NF-kB misc MPG misc T.peptide |
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Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells |
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Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells |
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Sivakumar, Allur Subramaniyan |
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Sivakumar, Allur Subramaniyan Ochirbat, Chinzorio Cho, Soo-Hyun Yang, Jieun Hwang, Inho |
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antiapoptotic effect of a novel synthetic peptide from bovine muscle and mpg peptide on $ h_{2} %$ o_{2} $-induced c2c12 cells |
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Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells |
| abstract |
Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. © The Society for In Vitro Biology 2014 |
| abstractGer |
Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. © The Society for In Vitro Biology 2014 |
| abstract_unstemmed |
Abstract The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+•) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against $ H_{2} %$ O_{2} $-induced C2C12 cells. $ H_{2} %$ O_{2} $-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-α and NF-κB are significantly (p < 0.05) increased in $ H_{2} %$ O_{2} $-induced C2C12 cells, whereas a significant decrease (p < 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p < 0.05) decrease the mRNA expression of caspase-3 in $ H_{2} %$ O_{2} $-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPH• and ABTS+• radical and inhibit cytotoxicity against $ H_{2} %$ O_{2} $-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides. © The Society for In Vitro Biology 2014 |
| collection_details |
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7 |
| title_short |
Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on $ H_{2} %$ O_{2} $-induced C2C12 cells |
| url |
https://dx.doi.org/10.1007/s11626-014-9745-2 |
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| author2 |
Ochirbat, Chinzorio Cho, Soo-Hyun Yang, Jieun Hwang, Inho |
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| doi_str |
10.1007/s11626-014-9745-2 |
| up_date |
2024-07-03T21:06:44.456Z |
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| score |
7.4006453 |