Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation
Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines w...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Parameswari, Chidambaram [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© The Society for In Vitro Biology 2010 |
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| Übergeordnetes Werk: |
Enthalten in: In vitro cellular & developmental biology - Berlin : Springer, 1991, 46(2010), 5 vom: 25. Sept., Seite 395-402 |
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| Übergeordnetes Werk: |
volume:46 ; year:2010 ; number:5 ; day:25 ; month:09 ; pages:395-402 |
| Links: |
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| DOI / URN: |
10.1007/s11627-010-9313-8 |
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| Katalog-ID: |
SPR021230277 |
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| 100 | 1 | |a Parameswari, Chidambaram |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation |
| 264 | 1 | |c 2010 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 520 | |a Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. | ||
| 650 | 4 | |a Albino plants |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Homozygous transgenic plants |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Segregation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a generation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Transgenic rice |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Sripriya, Rajasekaran |4 aut | |
| 700 | 1 | |a Veluthambi, Karuppannan |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t In vitro cellular & developmental biology |d Berlin : Springer, 1991 |g 46(2010), 5 vom: 25. Sept., Seite 395-402 |w (DE-627)306365367 |w (DE-600)1497464-2 |x 1475-2689 |7 nnns |
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| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
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10.1007/s11627-010-9313-8 doi (DE-627)SPR021230277 (SPR)s11627-010-9313-8-e DE-627 ger DE-627 rakwb eng Parameswari, Chidambaram verfasserin aut Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2010 Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. Albino plants (dpeaa)DE-He213 Homozygous transgenic plants (dpeaa)DE-He213 Segregation (dpeaa)DE-He213 generation (dpeaa)DE-He213 Transgenic rice (dpeaa)DE-He213 Sripriya, Rajasekaran aut Veluthambi, Karuppannan aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 46(2010), 5 vom: 25. Sept., Seite 395-402 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:46 year:2010 number:5 day:25 month:09 pages:395-402 https://dx.doi.org/10.1007/s11627-010-9313-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 46 2010 5 25 09 395-402 |
| spelling |
10.1007/s11627-010-9313-8 doi (DE-627)SPR021230277 (SPR)s11627-010-9313-8-e DE-627 ger DE-627 rakwb eng Parameswari, Chidambaram verfasserin aut Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2010 Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. Albino plants (dpeaa)DE-He213 Homozygous transgenic plants (dpeaa)DE-He213 Segregation (dpeaa)DE-He213 generation (dpeaa)DE-He213 Transgenic rice (dpeaa)DE-He213 Sripriya, Rajasekaran aut Veluthambi, Karuppannan aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 46(2010), 5 vom: 25. Sept., Seite 395-402 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:46 year:2010 number:5 day:25 month:09 pages:395-402 https://dx.doi.org/10.1007/s11627-010-9313-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 46 2010 5 25 09 395-402 |
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10.1007/s11627-010-9313-8 doi (DE-627)SPR021230277 (SPR)s11627-010-9313-8-e DE-627 ger DE-627 rakwb eng Parameswari, Chidambaram verfasserin aut Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2010 Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. Albino plants (dpeaa)DE-He213 Homozygous transgenic plants (dpeaa)DE-He213 Segregation (dpeaa)DE-He213 generation (dpeaa)DE-He213 Transgenic rice (dpeaa)DE-He213 Sripriya, Rajasekaran aut Veluthambi, Karuppannan aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 46(2010), 5 vom: 25. Sept., Seite 395-402 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:46 year:2010 number:5 day:25 month:09 pages:395-402 https://dx.doi.org/10.1007/s11627-010-9313-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 46 2010 5 25 09 395-402 |
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10.1007/s11627-010-9313-8 doi (DE-627)SPR021230277 (SPR)s11627-010-9313-8-e DE-627 ger DE-627 rakwb eng Parameswari, Chidambaram verfasserin aut Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2010 Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. Albino plants (dpeaa)DE-He213 Homozygous transgenic plants (dpeaa)DE-He213 Segregation (dpeaa)DE-He213 generation (dpeaa)DE-He213 Transgenic rice (dpeaa)DE-He213 Sripriya, Rajasekaran aut Veluthambi, Karuppannan aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 46(2010), 5 vom: 25. Sept., Seite 395-402 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:46 year:2010 number:5 day:25 month:09 pages:395-402 https://dx.doi.org/10.1007/s11627-010-9313-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 46 2010 5 25 09 395-402 |
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10.1007/s11627-010-9313-8 doi (DE-627)SPR021230277 (SPR)s11627-010-9313-8-e DE-627 ger DE-627 rakwb eng Parameswari, Chidambaram verfasserin aut Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2010 Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. Albino plants (dpeaa)DE-He213 Homozygous transgenic plants (dpeaa)DE-He213 Segregation (dpeaa)DE-He213 generation (dpeaa)DE-He213 Transgenic rice (dpeaa)DE-He213 Sripriya, Rajasekaran aut Veluthambi, Karuppannan aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 46(2010), 5 vom: 25. Sept., Seite 395-402 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:46 year:2010 number:5 day:25 month:09 pages:395-402 https://dx.doi.org/10.1007/s11627-010-9313-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 46 2010 5 25 09 395-402 |
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English |
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Enthalten in In vitro cellular & developmental biology 46(2010), 5 vom: 25. Sept., Seite 395-402 volume:46 year:2010 number:5 day:25 month:09 pages:395-402 |
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Enthalten in In vitro cellular & developmental biology 46(2010), 5 vom: 25. Sept., Seite 395-402 volume:46 year:2010 number:5 day:25 month:09 pages:395-402 |
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Albino plants Homozygous transgenic plants Segregation generation Transgenic rice |
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In vitro cellular & developmental biology |
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Parameswari, Chidambaram @@aut@@ Sripriya, Rajasekaran @@aut@@ Veluthambi, Karuppannan @@aut@@ |
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2010-09-25T00:00:00Z |
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While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. 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Parameswari, Chidambaram |
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Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation Albino plants (dpeaa)DE-He213 Homozygous transgenic plants (dpeaa)DE-He213 Segregation (dpeaa)DE-He213 generation (dpeaa)DE-He213 Transgenic rice (dpeaa)DE-He213 |
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Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation |
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Parameswari, Chidambaram |
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Parameswari, Chidambaram Sripriya, Rajasekaran Veluthambi, Karuppannan |
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establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation |
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Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation |
| abstract |
Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. © The Society for In Vitro Biology 2010 |
| abstractGer |
Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. © The Society for In Vitro Biology 2010 |
| abstract_unstemmed |
Abstract In an analysis of 339 independent T0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the T1 generation. The albino phenotype segregated as a monogenic recessive trait. Southern blot analysis of the green and albino plants in the T1 generation confirmed that the albino trait and the T-DNA insertion events were unlinked. Segregation of the albino trait from the transgenic trait in the lines O54 and O36 was confirmed in T2 and T3 generations, respectively. Homozygous transgenic plants free from the albino trait were also identified. In the double-copy transgenic line C18, we genetically separated the two transgenic loci, out-segregated the albino locus from both transgene loci, and identified homozygous plants for each of the transgenic events by Southern blot analysis in the T1 generation itself. Thus, we demonstrate that when an albino trait appears in the T1 generation and is unlinked to a transgene locus, the albino locus can be segregated from the transgene locus and homozygous transgenic lines free from albinos can be established. © The Society for In Vitro Biology 2010 |
| collection_details |
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| container_issue |
5 |
| title_short |
Establishment of homozygous transgenic rice lines after elimination of unlinked albino traits from transgenic events through segregation |
| url |
https://dx.doi.org/10.1007/s11627-010-9313-8 |
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| author2 |
Sripriya, Rajasekaran Veluthambi, Karuppannan |
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Sripriya, Rajasekaran Veluthambi, Karuppannan |
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| doi_str |
10.1007/s11627-010-9313-8 |
| up_date |
2024-07-03T21:13:01.154Z |
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| score |
7.400551 |