Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.)
Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzyl...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kumar, Sandopu Sravan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
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| Anmerkung: |
© The Society for In Vitro Biology 2016 |
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| Übergeordnetes Werk: |
Enthalten in: In vitro cellular & developmental biology - Berlin : Springer, 1991, 52(2016), 4 vom: 26. Aug., Seite 427-436 |
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| Übergeordnetes Werk: |
volume:52 ; year:2016 ; number:4 ; day:26 ; month:08 ; pages:427-436 |
| Links: |
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| DOI / URN: |
10.1007/s11627-016-9785-2 |
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| Katalog-ID: |
SPR021235554 |
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| 100 | 1 | |a Kumar, Sandopu Sravan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) |
| 264 | 1 | |c 2016 | |
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| 520 | |a Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). | ||
| 650 | 4 | |a Micropropagation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Nodal explants |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Multiple shoots |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a rooting |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Silver nitrate |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Triacontanol |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Manoj, Prabhakaran |4 aut | |
| 700 | 1 | |a Giridhar, Parvatam |4 aut | |
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| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
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10.1007/s11627-016-9785-2 doi (DE-627)SPR021235554 (SPR)s11627-016-9785-2-e DE-627 ger DE-627 rakwb eng Kumar, Sandopu Sravan verfasserin aut Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2016 Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). Micropropagation (dpeaa)DE-He213 Nodal explants (dpeaa)DE-He213 Multiple shoots (dpeaa)DE-He213 rooting (dpeaa)DE-He213 Silver nitrate (dpeaa)DE-He213 Triacontanol (dpeaa)DE-He213 Manoj, Prabhakaran aut Giridhar, Parvatam aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 52(2016), 4 vom: 26. Aug., Seite 427-436 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:52 year:2016 number:4 day:26 month:08 pages:427-436 https://dx.doi.org/10.1007/s11627-016-9785-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 52 2016 4 26 08 427-436 |
| spelling |
10.1007/s11627-016-9785-2 doi (DE-627)SPR021235554 (SPR)s11627-016-9785-2-e DE-627 ger DE-627 rakwb eng Kumar, Sandopu Sravan verfasserin aut Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2016 Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). Micropropagation (dpeaa)DE-He213 Nodal explants (dpeaa)DE-He213 Multiple shoots (dpeaa)DE-He213 rooting (dpeaa)DE-He213 Silver nitrate (dpeaa)DE-He213 Triacontanol (dpeaa)DE-He213 Manoj, Prabhakaran aut Giridhar, Parvatam aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 52(2016), 4 vom: 26. Aug., Seite 427-436 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:52 year:2016 number:4 day:26 month:08 pages:427-436 https://dx.doi.org/10.1007/s11627-016-9785-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 52 2016 4 26 08 427-436 |
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10.1007/s11627-016-9785-2 doi (DE-627)SPR021235554 (SPR)s11627-016-9785-2-e DE-627 ger DE-627 rakwb eng Kumar, Sandopu Sravan verfasserin aut Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2016 Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). Micropropagation (dpeaa)DE-He213 Nodal explants (dpeaa)DE-He213 Multiple shoots (dpeaa)DE-He213 rooting (dpeaa)DE-He213 Silver nitrate (dpeaa)DE-He213 Triacontanol (dpeaa)DE-He213 Manoj, Prabhakaran aut Giridhar, Parvatam aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 52(2016), 4 vom: 26. Aug., Seite 427-436 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:52 year:2016 number:4 day:26 month:08 pages:427-436 https://dx.doi.org/10.1007/s11627-016-9785-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 52 2016 4 26 08 427-436 |
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10.1007/s11627-016-9785-2 doi (DE-627)SPR021235554 (SPR)s11627-016-9785-2-e DE-627 ger DE-627 rakwb eng Kumar, Sandopu Sravan verfasserin aut Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2016 Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). Micropropagation (dpeaa)DE-He213 Nodal explants (dpeaa)DE-He213 Multiple shoots (dpeaa)DE-He213 rooting (dpeaa)DE-He213 Silver nitrate (dpeaa)DE-He213 Triacontanol (dpeaa)DE-He213 Manoj, Prabhakaran aut Giridhar, Parvatam aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 52(2016), 4 vom: 26. Aug., Seite 427-436 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:52 year:2016 number:4 day:26 month:08 pages:427-436 https://dx.doi.org/10.1007/s11627-016-9785-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 52 2016 4 26 08 427-436 |
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10.1007/s11627-016-9785-2 doi (DE-627)SPR021235554 (SPR)s11627-016-9785-2-e DE-627 ger DE-627 rakwb eng Kumar, Sandopu Sravan verfasserin aut Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Society for In Vitro Biology 2016 Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). Micropropagation (dpeaa)DE-He213 Nodal explants (dpeaa)DE-He213 Multiple shoots (dpeaa)DE-He213 rooting (dpeaa)DE-He213 Silver nitrate (dpeaa)DE-He213 Triacontanol (dpeaa)DE-He213 Manoj, Prabhakaran aut Giridhar, Parvatam aut Enthalten in In vitro cellular & developmental biology Berlin : Springer, 1991 52(2016), 4 vom: 26. Aug., Seite 427-436 (DE-627)306365367 (DE-600)1497464-2 1475-2689 nnns volume:52 year:2016 number:4 day:26 month:08 pages:427-436 https://dx.doi.org/10.1007/s11627-016-9785-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2939 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 52 2016 4 26 08 427-436 |
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Enthalten in In vitro cellular & developmental biology 52(2016), 4 vom: 26. Aug., Seite 427-436 volume:52 year:2016 number:4 day:26 month:08 pages:427-436 |
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Enthalten in In vitro cellular & developmental biology 52(2016), 4 vom: 26. Aug., Seite 427-436 volume:52 year:2016 number:4 day:26 month:08 pages:427-436 |
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Kumar, Sandopu Sravan @@aut@@ Manoj, Prabhakaran @@aut@@ Giridhar, Parvatam @@aut@@ |
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Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight).</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Micropropagation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nodal explants</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Multiple shoots</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">rooting</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver nitrate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Triacontanol</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Manoj, Prabhakaran</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Giridhar, Parvatam</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">In vitro cellular & developmental biology</subfield><subfield code="d">Berlin : Springer, 1991</subfield><subfield code="g">52(2016), 4 vom: 26. 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Kumar, Sandopu Sravan |
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Kumar, Sandopu Sravan misc Micropropagation misc Nodal explants misc Multiple shoots misc rooting misc Silver nitrate misc Triacontanol Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) |
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Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) Micropropagation (dpeaa)DE-He213 Nodal explants (dpeaa)DE-He213 Multiple shoots (dpeaa)DE-He213 rooting (dpeaa)DE-He213 Silver nitrate (dpeaa)DE-He213 Triacontanol (dpeaa)DE-He213 |
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Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) |
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Elektronische Aufsätze |
| author-letter |
Kumar, Sandopu Sravan |
| doi_str_mv |
10.1007/s11627-016-9785-2 |
| title_sort |
micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (hibiscus sabdariffa l.) |
| title_auth |
Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) |
| abstract |
Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). © The Society for In Vitro Biology 2016 |
| abstractGer |
Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). © The Society for In Vitro Biology 2016 |
| abstract_unstemmed |
Abstract An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg $ L^{−1} $ 6-benzylaminopurine, 0.5 mg $ L^{−1} $ indole-3-acetic acid, and 10 μM silver nitrate ($ AgNO_{3} $; 7–8 shoots/explant) or 20 μg $ L^{−1} $ triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of $ AgNO_{3} $ that was supplemented with 0.1–0.5 mg $ L^{−1} $ gibberellic acid ($ GA_{3} $) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg $ L^{−1} $ $ GA_{3} $ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg $ L^{−1} $ $ GA_{3} $, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from $ AgNO_{3} $- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg $ g^{−1} $ fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg $ g^{−1} $ fresh weight). © The Society for In Vitro Biology 2016 |
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| title_short |
Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.) |
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https://dx.doi.org/10.1007/s11627-016-9785-2 |
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Manoj, Prabhakaran Giridhar, Parvatam |
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| score |
7.397566 |