Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice
Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally admin...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chu, Deyong [verfasserIn] Li, Conglei [verfasserIn] Wu, Qiang [verfasserIn] Shen, Jilong [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Frontiers of medicine in China - [S.l.] : Higher Education Press, 2007, 2(2008), 2 vom: 29. Mai, Seite 154-165 |
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| Übergeordnetes Werk: |
volume:2 ; year:2008 ; number:2 ; day:29 ; month:05 ; pages:154-165 |
| Links: |
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| DOI / URN: |
10.1007/s11684-008-0029-7 |
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| Katalog-ID: |
SPR021722781 |
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| 245 | 1 | 0 | |a Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice |
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| 520 | |a Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. | ||
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| 650 | 4 | |a liver cirrhosis |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a hepatic stellate cell |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Wu, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Jilong |e verfasserin |4 aut | |
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10.1007/s11684-008-0029-7 doi (DE-627)SPR021722781 (SPR)s11684-008-0029-7-e DE-627 ger DE-627 rakwb eng 610 ASE Chu, Deyong verfasserin aut Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. paeoniflorin (dpeaa)DE-He213 liver cirrhosis (dpeaa)DE-He213 transforming growth factor β1 (dpeaa)DE-He213 macrophages (dpeaa)DE-He213 hepatic stellate cell (dpeaa)DE-He213 Li, Conglei verfasserin aut Wu, Qiang verfasserin aut Shen, Jilong verfasserin aut Enthalten in Frontiers of medicine in China [S.l.] : Higher Education Press, 2007 2(2008), 2 vom: 29. Mai, Seite 154-165 (DE-627)54578378X (DE-600)2388216-5 1673-7458 nnns volume:2 year:2008 number:2 day:29 month:05 pages:154-165 https://dx.doi.org/10.1007/s11684-008-0029-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 2 2008 2 29 05 154-165 |
| spelling |
10.1007/s11684-008-0029-7 doi (DE-627)SPR021722781 (SPR)s11684-008-0029-7-e DE-627 ger DE-627 rakwb eng 610 ASE Chu, Deyong verfasserin aut Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. paeoniflorin (dpeaa)DE-He213 liver cirrhosis (dpeaa)DE-He213 transforming growth factor β1 (dpeaa)DE-He213 macrophages (dpeaa)DE-He213 hepatic stellate cell (dpeaa)DE-He213 Li, Conglei verfasserin aut Wu, Qiang verfasserin aut Shen, Jilong verfasserin aut Enthalten in Frontiers of medicine in China [S.l.] : Higher Education Press, 2007 2(2008), 2 vom: 29. Mai, Seite 154-165 (DE-627)54578378X (DE-600)2388216-5 1673-7458 nnns volume:2 year:2008 number:2 day:29 month:05 pages:154-165 https://dx.doi.org/10.1007/s11684-008-0029-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 2 2008 2 29 05 154-165 |
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10.1007/s11684-008-0029-7 doi (DE-627)SPR021722781 (SPR)s11684-008-0029-7-e DE-627 ger DE-627 rakwb eng 610 ASE Chu, Deyong verfasserin aut Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. paeoniflorin (dpeaa)DE-He213 liver cirrhosis (dpeaa)DE-He213 transforming growth factor β1 (dpeaa)DE-He213 macrophages (dpeaa)DE-He213 hepatic stellate cell (dpeaa)DE-He213 Li, Conglei verfasserin aut Wu, Qiang verfasserin aut Shen, Jilong verfasserin aut Enthalten in Frontiers of medicine in China [S.l.] : Higher Education Press, 2007 2(2008), 2 vom: 29. Mai, Seite 154-165 (DE-627)54578378X (DE-600)2388216-5 1673-7458 nnns volume:2 year:2008 number:2 day:29 month:05 pages:154-165 https://dx.doi.org/10.1007/s11684-008-0029-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 2 2008 2 29 05 154-165 |
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10.1007/s11684-008-0029-7 doi (DE-627)SPR021722781 (SPR)s11684-008-0029-7-e DE-627 ger DE-627 rakwb eng 610 ASE Chu, Deyong verfasserin aut Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. paeoniflorin (dpeaa)DE-He213 liver cirrhosis (dpeaa)DE-He213 transforming growth factor β1 (dpeaa)DE-He213 macrophages (dpeaa)DE-He213 hepatic stellate cell (dpeaa)DE-He213 Li, Conglei verfasserin aut Wu, Qiang verfasserin aut Shen, Jilong verfasserin aut Enthalten in Frontiers of medicine in China [S.l.] : Higher Education Press, 2007 2(2008), 2 vom: 29. Mai, Seite 154-165 (DE-627)54578378X (DE-600)2388216-5 1673-7458 nnns volume:2 year:2008 number:2 day:29 month:05 pages:154-165 https://dx.doi.org/10.1007/s11684-008-0029-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 2 2008 2 29 05 154-165 |
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10.1007/s11684-008-0029-7 doi (DE-627)SPR021722781 (SPR)s11684-008-0029-7-e DE-627 ger DE-627 rakwb eng 610 ASE Chu, Deyong verfasserin aut Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. paeoniflorin (dpeaa)DE-He213 liver cirrhosis (dpeaa)DE-He213 transforming growth factor β1 (dpeaa)DE-He213 macrophages (dpeaa)DE-He213 hepatic stellate cell (dpeaa)DE-He213 Li, Conglei verfasserin aut Wu, Qiang verfasserin aut Shen, Jilong verfasserin aut Enthalten in Frontiers of medicine in China [S.l.] : Higher Education Press, 2007 2(2008), 2 vom: 29. Mai, Seite 154-165 (DE-627)54578378X (DE-600)2388216-5 1673-7458 nnns volume:2 year:2008 number:2 day:29 month:05 pages:154-165 https://dx.doi.org/10.1007/s11684-008-0029-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 2 2008 2 29 05 154-165 |
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English |
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Enthalten in Frontiers of medicine in China 2(2008), 2 vom: 29. Mai, Seite 154-165 volume:2 year:2008 number:2 day:29 month:05 pages:154-165 |
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Enthalten in Frontiers of medicine in China 2(2008), 2 vom: 29. Mai, Seite 154-165 volume:2 year:2008 number:2 day:29 month:05 pages:154-165 |
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paeoniflorin liver cirrhosis transforming growth factor β1 macrophages hepatic stellate cell |
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Frontiers of medicine in China |
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Chu, Deyong @@aut@@ Li, Conglei @@aut@@ Wu, Qiang @@aut@@ Shen, Jilong @@aut@@ |
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2008-05-29T00:00:00Z |
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Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. 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Chu, Deyong |
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Chu, Deyong ddc 610 misc paeoniflorin misc liver cirrhosis misc transforming growth factor β1 misc macrophages misc hepatic stellate cell Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice |
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610 ASE Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice paeoniflorin (dpeaa)DE-He213 liver cirrhosis (dpeaa)DE-He213 transforming growth factor β1 (dpeaa)DE-He213 macrophages (dpeaa)DE-He213 hepatic stellate cell (dpeaa)DE-He213 |
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ddc 610 misc paeoniflorin misc liver cirrhosis misc transforming growth factor β1 misc macrophages misc hepatic stellate cell |
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Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice |
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Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice |
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Chu, Deyong Li, Conglei Wu, Qiang Shen, Jilong |
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paeoniflorin prevents hepatic fibrosis of schistosomiasis japonica by inhibiting tgf-β1 production from macrophages in mice |
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Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice |
| abstract |
Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. |
| abstractGer |
Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. |
| abstract_unstemmed |
Abstract In order to investigate the effect of paeoniflorin (PAE) on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro, a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum. Then, PAE was orally administered before and after praziquantel treatment and both therapeutics were given simultaneously at different time points after the infection. The concentration of serum hyaluronic acid (HA) was determined by radioimmunoassay (RIA). Hepatic granuloma and fibrosis were evaluated via HE and Masson staining. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and collagen I (Col I) protein was detected by immunohistochemistry. The effect of soluble egg antigen (SEA) and PAE on the production of TGF-β1 from mouse peritoneal macrophages (PMϕs) was investigated by RT-PCR, Western blotting and ELISA. The effect of TGF-β1 in optimum macrophage-conditioned medium (OPMCM) on the proliferation of hepatic stellate cells (HSCs) and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA. The results show that PAE could significantly reduce the concentration of serum HA, the size of egg granuloma, the severity of hepatic fibrosis and the expression of α-SMA, TGF-β1 and Col I protein in the pre-treatment group. However, in sim-or post-treatment group, PAE did not have any significant therapeutic effect. TGF-β1 could be secreted from PMϕs stimulated by SEA. Meanwhile, the production of TGF-β1 from PMϕs could be depressed significantly by PAE in a concentration-dependent manner. TGF-β1 could promote the proliferation of HSCs and the secretion of collagens. In a word, PAE can prevent hepatic granuloma and fibrosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMϕs, the proliferation and activation of HSCs and the secretion of collagens from HSCs. |
| collection_details |
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| container_issue |
2 |
| title_short |
Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice |
| url |
https://dx.doi.org/10.1007/s11684-008-0029-7 |
| remote_bool |
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| author2 |
Li, Conglei Wu, Qiang Shen, Jilong |
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| doi_str |
10.1007/s11684-008-0029-7 |
| up_date |
2024-07-04T00:05:43.163Z |
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| score |
7.4022093 |