Construction of an amperometric polyphenol biosensor based on PVA membrane
Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Narang, Jagriti [verfasserIn] Chawla, Sheetal [verfasserIn] Chauhan, Nidhi [verfasserIn] Dahiya, Monika [verfasserIn] Pundir, Chandra Shekhar [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Sensing and instrumentation for food quality and safety - New York, NY : Springer, 2007, 7(2013), 1 vom: 04. Jan., Seite 22-28 |
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| Übergeordnetes Werk: |
volume:7 ; year:2013 ; number:1 ; day:04 ; month:01 ; pages:22-28 |
| Links: |
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| DOI / URN: |
10.1007/s11694-012-9135-y |
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SPR021766177 |
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| 520 | |a Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). | ||
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| 700 | 1 | |a Chauhan, Nidhi |e verfasserin |4 aut | |
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| 700 | 1 | |a Pundir, Chandra Shekhar |e verfasserin |4 aut | |
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10.1007/s11694-012-9135-y doi (DE-627)SPR021766177 (SPR)s11694-012-9135-y-e DE-627 ger DE-627 rakwb eng 630 640 ASE Narang, Jagriti verfasserin aut Construction of an amperometric polyphenol biosensor based on PVA membrane 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). Polyphenol (dpeaa)DE-He213 Polyphenol oxidase (dpeaa)DE-He213 PVA membrane (dpeaa)DE-He213 Amperometric biosensor (dpeaa)DE-He213 L-DOPA (dpeaa)DE-He213 Chawla, Sheetal verfasserin aut Chauhan, Nidhi verfasserin aut Dahiya, Monika verfasserin aut Pundir, Chandra Shekhar verfasserin aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 7(2013), 1 vom: 04. Jan., Seite 22-28 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:7 year:2013 number:1 day:04 month:01 pages:22-28 https://dx.doi.org/10.1007/s11694-012-9135-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 7 2013 1 04 01 22-28 |
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10.1007/s11694-012-9135-y doi (DE-627)SPR021766177 (SPR)s11694-012-9135-y-e DE-627 ger DE-627 rakwb eng 630 640 ASE Narang, Jagriti verfasserin aut Construction of an amperometric polyphenol biosensor based on PVA membrane 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). Polyphenol (dpeaa)DE-He213 Polyphenol oxidase (dpeaa)DE-He213 PVA membrane (dpeaa)DE-He213 Amperometric biosensor (dpeaa)DE-He213 L-DOPA (dpeaa)DE-He213 Chawla, Sheetal verfasserin aut Chauhan, Nidhi verfasserin aut Dahiya, Monika verfasserin aut Pundir, Chandra Shekhar verfasserin aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 7(2013), 1 vom: 04. Jan., Seite 22-28 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:7 year:2013 number:1 day:04 month:01 pages:22-28 https://dx.doi.org/10.1007/s11694-012-9135-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 7 2013 1 04 01 22-28 |
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10.1007/s11694-012-9135-y doi (DE-627)SPR021766177 (SPR)s11694-012-9135-y-e DE-627 ger DE-627 rakwb eng 630 640 ASE Narang, Jagriti verfasserin aut Construction of an amperometric polyphenol biosensor based on PVA membrane 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). Polyphenol (dpeaa)DE-He213 Polyphenol oxidase (dpeaa)DE-He213 PVA membrane (dpeaa)DE-He213 Amperometric biosensor (dpeaa)DE-He213 L-DOPA (dpeaa)DE-He213 Chawla, Sheetal verfasserin aut Chauhan, Nidhi verfasserin aut Dahiya, Monika verfasserin aut Pundir, Chandra Shekhar verfasserin aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 7(2013), 1 vom: 04. Jan., Seite 22-28 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:7 year:2013 number:1 day:04 month:01 pages:22-28 https://dx.doi.org/10.1007/s11694-012-9135-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 7 2013 1 04 01 22-28 |
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10.1007/s11694-012-9135-y doi (DE-627)SPR021766177 (SPR)s11694-012-9135-y-e DE-627 ger DE-627 rakwb eng 630 640 ASE Narang, Jagriti verfasserin aut Construction of an amperometric polyphenol biosensor based on PVA membrane 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). Polyphenol (dpeaa)DE-He213 Polyphenol oxidase (dpeaa)DE-He213 PVA membrane (dpeaa)DE-He213 Amperometric biosensor (dpeaa)DE-He213 L-DOPA (dpeaa)DE-He213 Chawla, Sheetal verfasserin aut Chauhan, Nidhi verfasserin aut Dahiya, Monika verfasserin aut Pundir, Chandra Shekhar verfasserin aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 7(2013), 1 vom: 04. Jan., Seite 22-28 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:7 year:2013 number:1 day:04 month:01 pages:22-28 https://dx.doi.org/10.1007/s11694-012-9135-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 7 2013 1 04 01 22-28 |
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10.1007/s11694-012-9135-y doi (DE-627)SPR021766177 (SPR)s11694-012-9135-y-e DE-627 ger DE-627 rakwb eng 630 640 ASE Narang, Jagriti verfasserin aut Construction of an amperometric polyphenol biosensor based on PVA membrane 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). Polyphenol (dpeaa)DE-He213 Polyphenol oxidase (dpeaa)DE-He213 PVA membrane (dpeaa)DE-He213 Amperometric biosensor (dpeaa)DE-He213 L-DOPA (dpeaa)DE-He213 Chawla, Sheetal verfasserin aut Chauhan, Nidhi verfasserin aut Dahiya, Monika verfasserin aut Pundir, Chandra Shekhar verfasserin aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 7(2013), 1 vom: 04. Jan., Seite 22-28 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:7 year:2013 number:1 day:04 month:01 pages:22-28 https://dx.doi.org/10.1007/s11694-012-9135-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 7 2013 1 04 01 22-28 |
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Enthalten in Sensing and instrumentation for food quality and safety 7(2013), 1 vom: 04. Jan., Seite 22-28 volume:7 year:2013 number:1 day:04 month:01 pages:22-28 |
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Enthalten in Sensing and instrumentation for food quality and safety 7(2013), 1 vom: 04. Jan., Seite 22-28 volume:7 year:2013 number:1 day:04 month:01 pages:22-28 |
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Narang, Jagriti @@aut@@ Chawla, Sheetal @@aut@@ Chauhan, Nidhi @@aut@@ Dahiya, Monika @@aut@@ Pundir, Chandra Shekhar @@aut@@ |
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Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). |
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Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). |
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Abstract A method is described for construction of an amperometric biosensor for determination of polyphenolic content using polyvinyl alcohol membrane bound polyphenol oxidase mounted on a gold coated Ag wire as a working electrode, a silver/silver chloride (Ag/AgCl) reference electrode and Pt wire as auxiliary electrode. The biosensor showed optimum response within 30 s, when operated at 30 °C. A linear relationship was obtained between L-DOPA concentration (0.5–20 μM) and current (μA). Limit of detection of the method was 0.5 μM. The biosensor measured polyphenols in tea leaves, alcoholic beverages and water. The enzyme electrode was used 280 times over 6 months, when stored at 4 °C. The biosensor is better than earlier membrane based biosensors in terms of detection limit (0.5 μM) and stability (6 months). |
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