Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27
Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and i...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Dutta, Subhasish [verfasserIn] Bhunia, Biswanath [verfasserIn] Raju, Anish [verfasserIn] Maity, Namrata [verfasserIn] Dey, Apurba [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2019 |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
Enthalten in: Chemical papers - Wien : Springer Vienna, 1947, 73(2019), 8 vom: 02. Apr., Seite 2053-2063 |
|---|---|
| Übergeordnetes Werk: |
volume:73 ; year:2019 ; number:8 ; day:02 ; month:04 ; pages:2053-2063 |
| Links: |
|---|
| DOI / URN: |
10.1007/s11696-019-00767-0 |
|---|
| Katalog-ID: |
SPR021886946 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR021886946 | ||
| 003 | DE-627 | ||
| 005 | 20230519143509.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201006s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11696-019-00767-0 |2 doi | |
| 035 | |a (DE-627)SPR021886946 | ||
| 035 | |a (SPR)s11696-019-00767-0-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 540 |q ASE |
| 084 | |a 35.00 |2 bkl | ||
| 100 | 1 | |a Dutta, Subhasish |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. | ||
| 650 | 4 | |a Rapamycin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Luedeking–Piret model |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Kinetics |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a LC–MS |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Bhunia, Biswanath |e verfasserin |4 aut | |
| 700 | 1 | |a Raju, Anish |e verfasserin |4 aut | |
| 700 | 1 | |a Maity, Namrata |e verfasserin |4 aut | |
| 700 | 1 | |a Dey, Apurba |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Chemical papers |d Wien : Springer Vienna, 1947 |g 73(2019), 8 vom: 02. Apr., Seite 2053-2063 |w (DE-627)518347737 |w (DE-600)2252770-9 |x 1336-9075 |7 nnns |
| 773 | 1 | 8 | |g volume:73 |g year:2019 |g number:8 |g day:02 |g month:04 |g pages:2053-2063 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s11696-019-00767-0 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a GBV_ILN_11 | ||
| 912 | |a GBV_ILN_20 | ||
| 912 | |a GBV_ILN_22 | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_65 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_100 | ||
| 912 | |a GBV_ILN_101 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_171 | ||
| 912 | |a GBV_ILN_187 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_250 | ||
| 912 | |a GBV_ILN_266 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
| 936 | b | k | |a 35.00 |q ASE |
| 951 | |a AR | ||
| 952 | |d 73 |j 2019 |e 8 |b 02 |c 04 |h 2053-2063 | ||
| author_variant |
s d sd b b bb a r ar n m nm a d ad |
|---|---|
| matchkey_str |
article:13369075:2019----::nacdaayipoutotruhieiaduiiaintdebmtnsrios |
| hierarchy_sort_str |
2019 |
| bklnumber |
35.00 |
| publishDate |
2019 |
| allfields |
10.1007/s11696-019-00767-0 doi (DE-627)SPR021886946 (SPR)s11696-019-00767-0-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Dutta, Subhasish verfasserin aut Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. Rapamycin (dpeaa)DE-He213 Luedeking–Piret model (dpeaa)DE-He213 Kinetics (dpeaa)DE-He213 LC–MS (dpeaa)DE-He213 Bhunia, Biswanath verfasserin aut Raju, Anish verfasserin aut Maity, Namrata verfasserin aut Dey, Apurba verfasserin aut Enthalten in Chemical papers Wien : Springer Vienna, 1947 73(2019), 8 vom: 02. Apr., Seite 2053-2063 (DE-627)518347737 (DE-600)2252770-9 1336-9075 nnns volume:73 year:2019 number:8 day:02 month:04 pages:2053-2063 https://dx.doi.org/10.1007/s11696-019-00767-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 73 2019 8 02 04 2053-2063 |
| spelling |
10.1007/s11696-019-00767-0 doi (DE-627)SPR021886946 (SPR)s11696-019-00767-0-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Dutta, Subhasish verfasserin aut Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. Rapamycin (dpeaa)DE-He213 Luedeking–Piret model (dpeaa)DE-He213 Kinetics (dpeaa)DE-He213 LC–MS (dpeaa)DE-He213 Bhunia, Biswanath verfasserin aut Raju, Anish verfasserin aut Maity, Namrata verfasserin aut Dey, Apurba verfasserin aut Enthalten in Chemical papers Wien : Springer Vienna, 1947 73(2019), 8 vom: 02. Apr., Seite 2053-2063 (DE-627)518347737 (DE-600)2252770-9 1336-9075 nnns volume:73 year:2019 number:8 day:02 month:04 pages:2053-2063 https://dx.doi.org/10.1007/s11696-019-00767-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 73 2019 8 02 04 2053-2063 |
| allfields_unstemmed |
10.1007/s11696-019-00767-0 doi (DE-627)SPR021886946 (SPR)s11696-019-00767-0-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Dutta, Subhasish verfasserin aut Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. Rapamycin (dpeaa)DE-He213 Luedeking–Piret model (dpeaa)DE-He213 Kinetics (dpeaa)DE-He213 LC–MS (dpeaa)DE-He213 Bhunia, Biswanath verfasserin aut Raju, Anish verfasserin aut Maity, Namrata verfasserin aut Dey, Apurba verfasserin aut Enthalten in Chemical papers Wien : Springer Vienna, 1947 73(2019), 8 vom: 02. Apr., Seite 2053-2063 (DE-627)518347737 (DE-600)2252770-9 1336-9075 nnns volume:73 year:2019 number:8 day:02 month:04 pages:2053-2063 https://dx.doi.org/10.1007/s11696-019-00767-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 73 2019 8 02 04 2053-2063 |
| allfieldsGer |
10.1007/s11696-019-00767-0 doi (DE-627)SPR021886946 (SPR)s11696-019-00767-0-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Dutta, Subhasish verfasserin aut Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. Rapamycin (dpeaa)DE-He213 Luedeking–Piret model (dpeaa)DE-He213 Kinetics (dpeaa)DE-He213 LC–MS (dpeaa)DE-He213 Bhunia, Biswanath verfasserin aut Raju, Anish verfasserin aut Maity, Namrata verfasserin aut Dey, Apurba verfasserin aut Enthalten in Chemical papers Wien : Springer Vienna, 1947 73(2019), 8 vom: 02. Apr., Seite 2053-2063 (DE-627)518347737 (DE-600)2252770-9 1336-9075 nnns volume:73 year:2019 number:8 day:02 month:04 pages:2053-2063 https://dx.doi.org/10.1007/s11696-019-00767-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 73 2019 8 02 04 2053-2063 |
| allfieldsSound |
10.1007/s11696-019-00767-0 doi (DE-627)SPR021886946 (SPR)s11696-019-00767-0-e DE-627 ger DE-627 rakwb eng 540 ASE 35.00 bkl Dutta, Subhasish verfasserin aut Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. Rapamycin (dpeaa)DE-He213 Luedeking–Piret model (dpeaa)DE-He213 Kinetics (dpeaa)DE-He213 LC–MS (dpeaa)DE-He213 Bhunia, Biswanath verfasserin aut Raju, Anish verfasserin aut Maity, Namrata verfasserin aut Dey, Apurba verfasserin aut Enthalten in Chemical papers Wien : Springer Vienna, 1947 73(2019), 8 vom: 02. Apr., Seite 2053-2063 (DE-627)518347737 (DE-600)2252770-9 1336-9075 nnns volume:73 year:2019 number:8 day:02 month:04 pages:2053-2063 https://dx.doi.org/10.1007/s11696-019-00767-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 35.00 ASE AR 73 2019 8 02 04 2053-2063 |
| language |
English |
| source |
Enthalten in Chemical papers 73(2019), 8 vom: 02. Apr., Seite 2053-2063 volume:73 year:2019 number:8 day:02 month:04 pages:2053-2063 |
| sourceStr |
Enthalten in Chemical papers 73(2019), 8 vom: 02. Apr., Seite 2053-2063 volume:73 year:2019 number:8 day:02 month:04 pages:2053-2063 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Rapamycin Luedeking–Piret model Kinetics LC–MS |
| dewey-raw |
540 |
| isfreeaccess_bool |
false |
| container_title |
Chemical papers |
| authorswithroles_txt_mv |
Dutta, Subhasish @@aut@@ Bhunia, Biswanath @@aut@@ Raju, Anish @@aut@@ Maity, Namrata @@aut@@ Dey, Apurba @@aut@@ |
| publishDateDaySort_date |
2019-04-02T00:00:00Z |
| hierarchy_top_id |
518347737 |
| dewey-sort |
3540 |
| id |
SPR021886946 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR021886946</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519143509.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201006s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s11696-019-00767-0</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR021886946</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s11696-019-00767-0-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">35.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Dutta, Subhasish</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Rapamycin</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Luedeking–Piret model</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Kinetics</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">LC–MS</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bhunia, Biswanath</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Raju, Anish</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Maity, Namrata</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dey, Apurba</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Chemical papers</subfield><subfield code="d">Wien : Springer Vienna, 1947</subfield><subfield code="g">73(2019), 8 vom: 02. Apr., Seite 2053-2063</subfield><subfield code="w">(DE-627)518347737</subfield><subfield code="w">(DE-600)2252770-9</subfield><subfield code="x">1336-9075</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:73</subfield><subfield code="g">year:2019</subfield><subfield code="g">number:8</subfield><subfield code="g">day:02</subfield><subfield code="g">month:04</subfield><subfield code="g">pages:2053-2063</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s11696-019-00767-0</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_120</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_138</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_171</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_187</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_250</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_266</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_281</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_636</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2093</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2107</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2188</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2446</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2472</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2548</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">35.00</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">73</subfield><subfield code="j">2019</subfield><subfield code="e">8</subfield><subfield code="b">02</subfield><subfield code="c">04</subfield><subfield code="h">2053-2063</subfield></datafield></record></collection>
|
| author |
Dutta, Subhasish |
| spellingShingle |
Dutta, Subhasish ddc 540 bkl 35.00 misc Rapamycin misc Luedeking–Piret model misc Kinetics misc LC–MS Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 |
| authorStr |
Dutta, Subhasish |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)518347737 |
| format |
electronic Article |
| dewey-ones |
540 - Chemistry & allied sciences |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut aut |
| collection |
springer |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1336-9075 |
| topic_title |
540 ASE 35.00 bkl Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 Rapamycin (dpeaa)DE-He213 Luedeking–Piret model (dpeaa)DE-He213 Kinetics (dpeaa)DE-He213 LC–MS (dpeaa)DE-He213 |
| topic |
ddc 540 bkl 35.00 misc Rapamycin misc Luedeking–Piret model misc Kinetics misc LC–MS |
| topic_unstemmed |
ddc 540 bkl 35.00 misc Rapamycin misc Luedeking–Piret model misc Kinetics misc LC–MS |
| topic_browse |
ddc 540 bkl 35.00 misc Rapamycin misc Luedeking–Piret model misc Kinetics misc LC–MS |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
Chemical papers |
| hierarchy_parent_id |
518347737 |
| dewey-tens |
540 - Chemistry |
| hierarchy_top_title |
Chemical papers |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)518347737 (DE-600)2252770-9 |
| title |
Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 |
| ctrlnum |
(DE-627)SPR021886946 (SPR)s11696-019-00767-0-e |
| title_full |
Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 |
| author_sort |
Dutta, Subhasish |
| journal |
Chemical papers |
| journalStr |
Chemical papers |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
500 - Science |
| recordtype |
marc |
| publishDateSort |
2019 |
| contenttype_str_mv |
txt |
| container_start_page |
2053 |
| author_browse |
Dutta, Subhasish Bhunia, Biswanath Raju, Anish Maity, Namrata Dey, Apurba |
| container_volume |
73 |
| class |
540 ASE 35.00 bkl |
| format_se |
Elektronische Aufsätze |
| author-letter |
Dutta, Subhasish |
| doi_str_mv |
10.1007/s11696-019-00767-0 |
| dewey-full |
540 |
| author2-role |
verfasserin |
| title_sort |
enhanced rapamycin production through kinetic and purification studies by mutant strain of streptomyces hygroscopicus ntg-30-27 |
| title_auth |
Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 |
| abstract |
Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. |
| abstractGer |
Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. |
| abstract_unstemmed |
Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 |
| container_issue |
8 |
| title_short |
Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27 |
| url |
https://dx.doi.org/10.1007/s11696-019-00767-0 |
| remote_bool |
true |
| author2 |
Bhunia, Biswanath Raju, Anish Maity, Namrata Dey, Apurba |
| author2Str |
Bhunia, Biswanath Raju, Anish Maity, Namrata Dey, Apurba |
| ppnlink |
518347737 |
| mediatype_str_mv |
c |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/s11696-019-00767-0 |
| up_date |
2024-07-04T00:52:43.836Z |
| _version_ |
1803607724615794688 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR021886946</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519143509.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201006s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s11696-019-00767-0</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR021886946</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s11696-019-00767-0-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">35.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Dutta, Subhasish</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Enhanced rapamycin production through kinetic and purification studies by mutant strain of Streptomyces hygroscopicus NTG-30-27</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Research work was implemented to describe the kinetics of cell growth, substrate utilization and product formation using a mutant strain of Streptomyces hygroscopicus NTG-30-27 in a 3-L bioreactor under optimized condition. Various substrate inhibition mathematical models were applied and it was found that the cell growth and substrate utilization kinetic data fitted well to those models. Andrew’s kinetic model was fitted very well (R2 = 0.998) with our experimental data among different models tested for analysis whereas Luedeking–Piret model suggested that our product is mixed growth associated. The values for maximum specific growth rate (µmax), doubling time (td), saturation constant (KS), inhibition constant (KI) and yield coefficient (YX/S) were found to be 0.03985 $ h^{−1} $, 17.16 h, 2.076 g/l, 0.009 g/l and 0.290 g $ g^{−1} $. Final rapamycin yield with mutant strain was found to be 531.4 mg/l on its 5th day of fermentation which is 6.7-fold higher than the wild type (79.31 mg/l). The effect of aeration on rapamycin production was studied by batch fermentation in a stirred tank reactor (STR) using S. hygroscopicus NTG-30-27. Dynamic behaviour and aeration efficiency of the reactor, as well as rheology pattern of the fermentation broth, were determined by calculating volumetric mass transfer coefficient (KLa) of the process using “Dynamic gassing out method”. KLa was found to be 54.53 $ h^{−1} $ which is quite significant for rapamycin production. Further purification and structural analysis of the extracted sample were carried out by liquid chromatography–mass spectrophotometry (LC–MS) technique in positive ionization mode and molecular mass was found to be 936 D. Finally, 90.62% purified rapamycin was obtained from the study.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Rapamycin</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Luedeking–Piret model</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Kinetics</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">LC–MS</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bhunia, Biswanath</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Raju, Anish</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Maity, Namrata</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dey, Apurba</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Chemical papers</subfield><subfield code="d">Wien : Springer Vienna, 1947</subfield><subfield code="g">73(2019), 8 vom: 02. Apr., Seite 2053-2063</subfield><subfield code="w">(DE-627)518347737</subfield><subfield code="w">(DE-600)2252770-9</subfield><subfield code="x">1336-9075</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:73</subfield><subfield code="g">year:2019</subfield><subfield code="g">number:8</subfield><subfield code="g">day:02</subfield><subfield code="g">month:04</subfield><subfield code="g">pages:2053-2063</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s11696-019-00767-0</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_120</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_138</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_171</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_187</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_250</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_266</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_281</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_636</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2093</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2107</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2188</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2446</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2472</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2548</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">35.00</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">73</subfield><subfield code="j">2019</subfield><subfield code="e">8</subfield><subfield code="b">02</subfield><subfield code="c">04</subfield><subfield code="h">2053-2063</subfield></datafield></record></collection>
|
| score |
7.400358 |