In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai

Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establish...
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Gespeichert in:

Autor*in:	Haq, Zafrul [verfasserIn] Kaloo, Z. A. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	micro propagation shoot multiplication nodal cuttings Murashige and Skoog

Übergeordnetes Werk:	Enthalten in: Frontiers of agriculture in China - [S.l.] : Higher Education Press, 2007, 4(2010), 3 vom: 30. Juni, Seite 358-361
Übergeordnetes Werk:	volume:4 ; year:2010 ; number:3 ; day:30 ; month:06 ; pages:358-361

Links:	Volltext

DOI / URN:	10.1007/s11703-010-1014-x

Katalog-ID:	SPR021931887

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allfields	10.1007/s11703-010-1014-x doi (DE-627)SPR021931887 (SPR)s11703-010-1014-x-e DE-627 ger DE-627 rakwb eng 630 640 ASE Haq, Zafrul verfasserin aut In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v). micro propagation (dpeaa)DE-He213 shoot multiplication (dpeaa)DE-He213 nodal cuttings (dpeaa)DE-He213 Murashige and Skoog (dpeaa)DE-He213 Kaloo, Z. A. verfasserin aut Enthalten in Frontiers of agriculture in China [S.l.] : Higher Education Press, 2007 4(2010), 3 vom: 30. Juni, Seite 358-361 (DE-627)538633018 (DE-600)2379672-8 1673-744X nnns volume:4 year:2010 number:3 day:30 month:06 pages:358-361 https://dx.doi.org/10.1007/s11703-010-1014-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_100 GBV_ILN_702 GBV_ILN_2009 GBV_ILN_2018 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4126 AR 4 2010 3 30 06 358-361
spelling	10.1007/s11703-010-1014-x doi (DE-627)SPR021931887 (SPR)s11703-010-1014-x-e DE-627 ger DE-627 rakwb eng 630 640 ASE Haq, Zafrul verfasserin aut In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v). micro propagation (dpeaa)DE-He213 shoot multiplication (dpeaa)DE-He213 nodal cuttings (dpeaa)DE-He213 Murashige and Skoog (dpeaa)DE-He213 Kaloo, Z. A. verfasserin aut Enthalten in Frontiers of agriculture in China [S.l.] : Higher Education Press, 2007 4(2010), 3 vom: 30. Juni, Seite 358-361 (DE-627)538633018 (DE-600)2379672-8 1673-744X nnns volume:4 year:2010 number:3 day:30 month:06 pages:358-361 https://dx.doi.org/10.1007/s11703-010-1014-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_100 GBV_ILN_702 GBV_ILN_2009 GBV_ILN_2018 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4126 AR 4 2010 3 30 06 358-361
allfields_unstemmed	10.1007/s11703-010-1014-x doi (DE-627)SPR021931887 (SPR)s11703-010-1014-x-e DE-627 ger DE-627 rakwb eng 630 640 ASE Haq, Zafrul verfasserin aut In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v). micro propagation (dpeaa)DE-He213 shoot multiplication (dpeaa)DE-He213 nodal cuttings (dpeaa)DE-He213 Murashige and Skoog (dpeaa)DE-He213 Kaloo, Z. A. verfasserin aut Enthalten in Frontiers of agriculture in China [S.l.] : Higher Education Press, 2007 4(2010), 3 vom: 30. Juni, Seite 358-361 (DE-627)538633018 (DE-600)2379672-8 1673-744X nnns volume:4 year:2010 number:3 day:30 month:06 pages:358-361 https://dx.doi.org/10.1007/s11703-010-1014-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_100 GBV_ILN_702 GBV_ILN_2009 GBV_ILN_2018 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4126 AR 4 2010 3 30 06 358-361
allfieldsGer	10.1007/s11703-010-1014-x doi (DE-627)SPR021931887 (SPR)s11703-010-1014-x-e DE-627 ger DE-627 rakwb eng 630 640 ASE Haq, Zafrul verfasserin aut In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v). micro propagation (dpeaa)DE-He213 shoot multiplication (dpeaa)DE-He213 nodal cuttings (dpeaa)DE-He213 Murashige and Skoog (dpeaa)DE-He213 Kaloo, Z. A. verfasserin aut Enthalten in Frontiers of agriculture in China [S.l.] : Higher Education Press, 2007 4(2010), 3 vom: 30. Juni, Seite 358-361 (DE-627)538633018 (DE-600)2379672-8 1673-744X nnns volume:4 year:2010 number:3 day:30 month:06 pages:358-361 https://dx.doi.org/10.1007/s11703-010-1014-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_100 GBV_ILN_702 GBV_ILN_2009 GBV_ILN_2018 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4126 AR 4 2010 3 30 06 358-361
allfieldsSound	10.1007/s11703-010-1014-x doi (DE-627)SPR021931887 (SPR)s11703-010-1014-x-e DE-627 ger DE-627 rakwb eng 630 640 ASE Haq, Zafrul verfasserin aut In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v). micro propagation (dpeaa)DE-He213 shoot multiplication (dpeaa)DE-He213 nodal cuttings (dpeaa)DE-He213 Murashige and Skoog (dpeaa)DE-He213 Kaloo, Z. A. verfasserin aut Enthalten in Frontiers of agriculture in China [S.l.] : Higher Education Press, 2007 4(2010), 3 vom: 30. Juni, Seite 358-361 (DE-627)538633018 (DE-600)2379672-8 1673-744X nnns volume:4 year:2010 number:3 day:30 month:06 pages:358-361 https://dx.doi.org/10.1007/s11703-010-1014-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_100 GBV_ILN_702 GBV_ILN_2009 GBV_ILN_2018 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4126 AR 4 2010 3 30 06 358-361
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author	Haq, Zafrul
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topic_title	630 640 ASE In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai micro propagation (dpeaa)DE-He213 shoot multiplication (dpeaa)DE-He213 nodal cuttings (dpeaa)DE-He213 Murashige and Skoog (dpeaa)DE-He213
topic	ddc 630 misc micro propagation misc shoot multiplication misc nodal cuttings misc Murashige and Skoog
topic_unstemmed	ddc 630 misc micro propagation misc shoot multiplication misc nodal cuttings misc Murashige and Skoog
topic_browse	ddc 630 misc micro propagation misc shoot multiplication misc nodal cuttings misc Murashige and Skoog
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hierarchy_parent_title	Frontiers of agriculture in China
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dewey-tens	630 - Agriculture 640 - Home & family management
hierarchy_top_title	Frontiers of agriculture in China
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title	In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai
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title_full	In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai
author_sort	Haq, Zafrul
journal	Frontiers of agriculture in China
journalStr	Frontiers of agriculture in China
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author_browse	Haq, Zafrul Kaloo, Z. A.
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class	630 640 ASE
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author-letter	Haq, Zafrul
doi_str_mv	10.1007/s11703-010-1014-x
dewey-full	630 640
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title_sort	in vitro micro propagation of “sand pear” pyrus pyrifolia (burm. f.) nakai
title_auth	In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai
abstract	Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v).
abstractGer	Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v).
abstract_unstemmed	Abstract This study was undertaken to standardize protocols for in vitro explant establishment, shoot multiplication and in vitro rooting for Pyrus pyrifolia, growing in the Kashmir valley. The explant used was nodal cuttings of 2–3cm size with one or two axillary buds. The highest explant establishment (over 90%) was observed during spring while the lowest (60%) explant establishment frequency was observed during winter season. The shoots sprouted from the axillary buds were excised and subcultured on MS basal medium. The highest shoot multiplication rate (130 shoots from a single nodal cutting) was obtained on MS medium supplemented with BAP 2.2 mg·$ L^{−1} $ and kinetin 1 mg·$ L^{−1} $ after four weeks. The shoots were inoculated for rooting in root induction medium of half strength MS salts supplemented with plant growth regulators like IBA, IAA, NAA, and 2, 4-D. However, the best rooting response (8.50 roots per shoot) was obtained in combination of hormones (IBA + NAA) 0.25 mg·$ L^{−1} $ each. The rooted micro shoots were acclimatized under decreasing humidity regime (95% RH to 75% RH over a period of 5±1 weeks). The highest ex vitro plantlet survival percentage (56.50%) of Pyrus pyrifolia was observed on sand + soil (1:1 v/v).
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title_short	In vitro micro propagation of “sand pear” Pyrus pyrifolia (Burm. f.) Nakai
url	https://dx.doi.org/10.1007/s11703-010-1014-x
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up_date	2024-07-04T01:04:49.342Z
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