$ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves
Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zhai, Jinling [verfasserIn] Xu, Haixia [verfasserIn] Cong, Xinli [verfasserIn] Deng, Yongchuan [verfasserIn] Xia, Zhihui [verfasserIn] Huang, Xi [verfasserIn] Hao, Gangping [verfasserIn] Jiang, Xingyu [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2012 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Acta physiologiae plantarum - Berlin : Springer, 1997, 35(2012), 1 vom: 25. Juli, Seite 161-173 |
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| Übergeordnetes Werk: |
volume:35 ; year:2012 ; number:1 ; day:25 ; month:07 ; pages:161-173 |
| Links: |
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| DOI / URN: |
10.1007/s11738-012-1059-y |
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| Katalog-ID: |
SPR022091033 |
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| 100 | 1 | |a Zhai, Jinling |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves |
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| 520 | |a Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. | ||
| 650 | 4 | |a Arabidopsis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Ca |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a /H |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a exchange |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Ca |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a -ATPase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a H |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a -ATPase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Plasma membrane |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Xu, Haixia |e verfasserin |4 aut | |
| 700 | 1 | |a Cong, Xinli |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Yongchuan |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Zhihui |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Gangping |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Xingyu |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Acta physiologiae plantarum |d Berlin : Springer, 1997 |g 35(2012), 1 vom: 25. Juli, Seite 161-173 |w (DE-627)516024906 |w (DE-600)2245807-4 |x 1861-1664 |7 nnns |
| 773 | 1 | 8 | |g volume:35 |g year:2012 |g number:1 |g day:25 |g month:07 |g pages:161-173 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s11738-012-1059-y |z lizenzpflichtig |3 Volltext |
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10.1007/s11738-012-1059-y doi (DE-627)SPR022091033 (SPR)s11738-012-1059-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.41 bkl Zhai, Jinling verfasserin aut $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. Arabidopsis (dpeaa)DE-He213 Ca (dpeaa)DE-He213 /H (dpeaa)DE-He213 exchange (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 H (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 Plasma membrane (dpeaa)DE-He213 Xu, Haixia verfasserin aut Cong, Xinli verfasserin aut Deng, Yongchuan verfasserin aut Xia, Zhihui verfasserin aut Huang, Xi verfasserin aut Hao, Gangping verfasserin aut Jiang, Xingyu verfasserin aut Enthalten in Acta physiologiae plantarum Berlin : Springer, 1997 35(2012), 1 vom: 25. Juli, Seite 161-173 (DE-627)516024906 (DE-600)2245807-4 1861-1664 nnns volume:35 year:2012 number:1 day:25 month:07 pages:161-173 https://dx.doi.org/10.1007/s11738-012-1059-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE AR 35 2012 1 25 07 161-173 |
| spelling |
10.1007/s11738-012-1059-y doi (DE-627)SPR022091033 (SPR)s11738-012-1059-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.41 bkl Zhai, Jinling verfasserin aut $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. Arabidopsis (dpeaa)DE-He213 Ca (dpeaa)DE-He213 /H (dpeaa)DE-He213 exchange (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 H (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 Plasma membrane (dpeaa)DE-He213 Xu, Haixia verfasserin aut Cong, Xinli verfasserin aut Deng, Yongchuan verfasserin aut Xia, Zhihui verfasserin aut Huang, Xi verfasserin aut Hao, Gangping verfasserin aut Jiang, Xingyu verfasserin aut Enthalten in Acta physiologiae plantarum Berlin : Springer, 1997 35(2012), 1 vom: 25. Juli, Seite 161-173 (DE-627)516024906 (DE-600)2245807-4 1861-1664 nnns volume:35 year:2012 number:1 day:25 month:07 pages:161-173 https://dx.doi.org/10.1007/s11738-012-1059-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE AR 35 2012 1 25 07 161-173 |
| allfields_unstemmed |
10.1007/s11738-012-1059-y doi (DE-627)SPR022091033 (SPR)s11738-012-1059-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.41 bkl Zhai, Jinling verfasserin aut $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. Arabidopsis (dpeaa)DE-He213 Ca (dpeaa)DE-He213 /H (dpeaa)DE-He213 exchange (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 H (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 Plasma membrane (dpeaa)DE-He213 Xu, Haixia verfasserin aut Cong, Xinli verfasserin aut Deng, Yongchuan verfasserin aut Xia, Zhihui verfasserin aut Huang, Xi verfasserin aut Hao, Gangping verfasserin aut Jiang, Xingyu verfasserin aut Enthalten in Acta physiologiae plantarum Berlin : Springer, 1997 35(2012), 1 vom: 25. Juli, Seite 161-173 (DE-627)516024906 (DE-600)2245807-4 1861-1664 nnns volume:35 year:2012 number:1 day:25 month:07 pages:161-173 https://dx.doi.org/10.1007/s11738-012-1059-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE AR 35 2012 1 25 07 161-173 |
| allfieldsGer |
10.1007/s11738-012-1059-y doi (DE-627)SPR022091033 (SPR)s11738-012-1059-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.41 bkl Zhai, Jinling verfasserin aut $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. Arabidopsis (dpeaa)DE-He213 Ca (dpeaa)DE-He213 /H (dpeaa)DE-He213 exchange (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 H (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 Plasma membrane (dpeaa)DE-He213 Xu, Haixia verfasserin aut Cong, Xinli verfasserin aut Deng, Yongchuan verfasserin aut Xia, Zhihui verfasserin aut Huang, Xi verfasserin aut Hao, Gangping verfasserin aut Jiang, Xingyu verfasserin aut Enthalten in Acta physiologiae plantarum Berlin : Springer, 1997 35(2012), 1 vom: 25. Juli, Seite 161-173 (DE-627)516024906 (DE-600)2245807-4 1861-1664 nnns volume:35 year:2012 number:1 day:25 month:07 pages:161-173 https://dx.doi.org/10.1007/s11738-012-1059-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE AR 35 2012 1 25 07 161-173 |
| allfieldsSound |
10.1007/s11738-012-1059-y doi (DE-627)SPR022091033 (SPR)s11738-012-1059-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.41 bkl Zhai, Jinling verfasserin aut $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. Arabidopsis (dpeaa)DE-He213 Ca (dpeaa)DE-He213 /H (dpeaa)DE-He213 exchange (dpeaa)DE-He213 Ca (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 H (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 Plasma membrane (dpeaa)DE-He213 Xu, Haixia verfasserin aut Cong, Xinli verfasserin aut Deng, Yongchuan verfasserin aut Xia, Zhihui verfasserin aut Huang, Xi verfasserin aut Hao, Gangping verfasserin aut Jiang, Xingyu verfasserin aut Enthalten in Acta physiologiae plantarum Berlin : Springer, 1997 35(2012), 1 vom: 25. Juli, Seite 161-173 (DE-627)516024906 (DE-600)2245807-4 1861-1664 nnns volume:35 year:2012 number:1 day:25 month:07 pages:161-173 https://dx.doi.org/10.1007/s11738-012-1059-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE AR 35 2012 1 25 07 161-173 |
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Enthalten in Acta physiologiae plantarum 35(2012), 1 vom: 25. Juli, Seite 161-173 volume:35 year:2012 number:1 day:25 month:07 pages:161-173 |
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Enthalten in Acta physiologiae plantarum 35(2012), 1 vom: 25. Juli, Seite 161-173 volume:35 year:2012 number:1 day:25 month:07 pages:161-173 |
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Arabidopsis Ca /H exchange -ATPase H Plasma membrane |
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Acta physiologiae plantarum |
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Zhai, Jinling @@aut@@ Xu, Haixia @@aut@@ Cong, Xinli @@aut@@ Deng, Yongchuan @@aut@@ Xia, Zhihui @@aut@@ Huang, Xi @@aut@@ Hao, Gangping @@aut@@ Jiang, Xingyu @@aut@@ |
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2012-07-25T00:00:00Z |
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Zhai, Jinling |
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Zhai, Jinling ddc 580 bkl 42.41 misc Arabidopsis misc Ca misc /H misc exchange misc -ATPase misc H misc Plasma membrane $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves |
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580 ASE 42.41 bkl $ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves Arabidopsis (dpeaa)DE-He213 Ca (dpeaa)DE-He213 /H (dpeaa)DE-He213 exchange (dpeaa)DE-He213 -ATPase (dpeaa)DE-He213 H (dpeaa)DE-He213 Plasma membrane (dpeaa)DE-He213 |
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ddc 580 bkl 42.41 misc Arabidopsis misc Ca misc /H misc exchange misc -ATPase misc H misc Plasma membrane |
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ddc 580 bkl 42.41 misc Arabidopsis misc Ca misc /H misc exchange misc -ATPase misc H misc Plasma membrane |
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$ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves |
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(DE-627)SPR022091033 (SPR)s11738-012-1059-y-e |
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$ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves |
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Zhai, Jinling |
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Acta physiologiae plantarum |
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Zhai, Jinling Xu, Haixia Cong, Xinli Deng, Yongchuan Xia, Zhihui Huang, Xi Hao, Gangping Jiang, Xingyu |
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$ ca^{2+} $/$ h^{+} $ exchange in the plasma membrane of arabidopsis thaliana leaves |
| title_auth |
$ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves |
| abstract |
Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. |
| abstractGer |
Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. |
| abstract_unstemmed |
Abstract A large number of plant $ Ca^{2+} $/$ H^{+} $ exchangers have been identified in endomembranes, but far fewer have been studied for $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane so far. To investigate the $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane vesicles was measured by $ Ca^{2+} $-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the $ Ca^{2+} $/$ H^{+} $ exchange was optimal at pH 7.0, and displayed transport specificity for $ Ca^{2+} $ with saturation kinetics at Km = 47 μM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the $ Ca^{2+} $-dependent transport of $ H^{+} $ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of $ Ca^{2+} $/$ H^{+} $ exchange increased comparing to control without valinomycin effect, suggesting that the $ Ca^{2+} $/$ H^{+} $ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than $ 2H^{+} $:$ Ca^{2+} $. Eosin Y, a $ Ca^{2+} $-ATPase inhibitor, drastically inhibited $ Ca^{2+} $/$ H^{+} $ exchange in plasma membrane as it does for the purified $ Ca^{2+} $-ATPase in proteoliposomes, indicating that measured $ Ca^{2+} $/$ H^{+} $ exchange activity is mainly due to a plasma membrane $ Ca^{2+} $ pump. These suggest that calcium ($ Ca^{2+} $) is transported out of Arabidopsis cells mainly through a $ Ca^{2+} $-ATPase-mediated $ Ca^{2+} $/$ H^{+} $ exchange system that is driven by the proton-motive force from the plasma membrane $ H^{+} $-ATPase. |
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$ Ca^{2+} $/$ H^{+} $ exchange in the plasma membrane of Arabidopsis thaliana leaves |
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