Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol
Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yang, Zhou-Xin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Anmerkung: |
© Institute of Molecular Biology, Slovak Academy of Sciences 2019 |
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| Übergeordnetes Werk: |
Enthalten in: Biologia - Berlin : De Gruyter, 1996, 74(2019), 10 vom: 12. Juni, Seite 1385-1394 |
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| Übergeordnetes Werk: |
volume:74 ; year:2019 ; number:10 ; day:12 ; month:06 ; pages:1385-1394 |
| Links: |
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| DOI / URN: |
10.2478/s11756-019-00288-x |
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| Katalog-ID: |
SPR022251855 |
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| 100 | 1 | |a Yang, Zhou-Xin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol |
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| 520 | |a Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. | ||
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| 650 | 4 | |a Poly (I:C) |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Inflammatory |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Transcriptome |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Dai, Ji-Huan |4 aut | |
| 700 | 1 | |a Chen, Sha-Sha |4 aut | |
| 700 | 1 | |a Yan, Jing |4 aut | |
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10.2478/s11756-019-00288-x doi (DE-627)SPR022251855 (SPR)s11756-019-00288-x-e DE-627 ger DE-627 rakwb eng Yang, Zhou-Xin verfasserin aut Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Institute of Molecular Biology, Slovak Academy of Sciences 2019 Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. Panaxadiol (dpeaa)DE-He213 Poly (I:C) (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 Transcriptome (dpeaa)DE-He213 Guo, Dong-Yang aut Shen, Ling-Zhi aut Mao, Gen-Xiang aut Dai, Ji-Huan aut Chen, Sha-Sha aut Yan, Jing aut Enthalten in Biologia Berlin : De Gruyter, 1996 74(2019), 10 vom: 12. Juni, Seite 1385-1394 (DE-627)518346137 (DE-600)2252542-7 1336-9563 nnns volume:74 year:2019 number:10 day:12 month:06 pages:1385-1394 https://dx.doi.org/10.2478/s11756-019-00288-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 74 2019 10 12 06 1385-1394 |
| spelling |
10.2478/s11756-019-00288-x doi (DE-627)SPR022251855 (SPR)s11756-019-00288-x-e DE-627 ger DE-627 rakwb eng Yang, Zhou-Xin verfasserin aut Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Institute of Molecular Biology, Slovak Academy of Sciences 2019 Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. Panaxadiol (dpeaa)DE-He213 Poly (I:C) (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 Transcriptome (dpeaa)DE-He213 Guo, Dong-Yang aut Shen, Ling-Zhi aut Mao, Gen-Xiang aut Dai, Ji-Huan aut Chen, Sha-Sha aut Yan, Jing aut Enthalten in Biologia Berlin : De Gruyter, 1996 74(2019), 10 vom: 12. Juni, Seite 1385-1394 (DE-627)518346137 (DE-600)2252542-7 1336-9563 nnns volume:74 year:2019 number:10 day:12 month:06 pages:1385-1394 https://dx.doi.org/10.2478/s11756-019-00288-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 74 2019 10 12 06 1385-1394 |
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10.2478/s11756-019-00288-x doi (DE-627)SPR022251855 (SPR)s11756-019-00288-x-e DE-627 ger DE-627 rakwb eng Yang, Zhou-Xin verfasserin aut Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Institute of Molecular Biology, Slovak Academy of Sciences 2019 Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. Panaxadiol (dpeaa)DE-He213 Poly (I:C) (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 Transcriptome (dpeaa)DE-He213 Guo, Dong-Yang aut Shen, Ling-Zhi aut Mao, Gen-Xiang aut Dai, Ji-Huan aut Chen, Sha-Sha aut Yan, Jing aut Enthalten in Biologia Berlin : De Gruyter, 1996 74(2019), 10 vom: 12. Juni, Seite 1385-1394 (DE-627)518346137 (DE-600)2252542-7 1336-9563 nnns volume:74 year:2019 number:10 day:12 month:06 pages:1385-1394 https://dx.doi.org/10.2478/s11756-019-00288-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 74 2019 10 12 06 1385-1394 |
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10.2478/s11756-019-00288-x doi (DE-627)SPR022251855 (SPR)s11756-019-00288-x-e DE-627 ger DE-627 rakwb eng Yang, Zhou-Xin verfasserin aut Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Institute of Molecular Biology, Slovak Academy of Sciences 2019 Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. Panaxadiol (dpeaa)DE-He213 Poly (I:C) (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 Transcriptome (dpeaa)DE-He213 Guo, Dong-Yang aut Shen, Ling-Zhi aut Mao, Gen-Xiang aut Dai, Ji-Huan aut Chen, Sha-Sha aut Yan, Jing aut Enthalten in Biologia Berlin : De Gruyter, 1996 74(2019), 10 vom: 12. Juni, Seite 1385-1394 (DE-627)518346137 (DE-600)2252542-7 1336-9563 nnns volume:74 year:2019 number:10 day:12 month:06 pages:1385-1394 https://dx.doi.org/10.2478/s11756-019-00288-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 74 2019 10 12 06 1385-1394 |
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10.2478/s11756-019-00288-x doi (DE-627)SPR022251855 (SPR)s11756-019-00288-x-e DE-627 ger DE-627 rakwb eng Yang, Zhou-Xin verfasserin aut Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Institute of Molecular Biology, Slovak Academy of Sciences 2019 Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. Panaxadiol (dpeaa)DE-He213 Poly (I:C) (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 Transcriptome (dpeaa)DE-He213 Guo, Dong-Yang aut Shen, Ling-Zhi aut Mao, Gen-Xiang aut Dai, Ji-Huan aut Chen, Sha-Sha aut Yan, Jing aut Enthalten in Biologia Berlin : De Gruyter, 1996 74(2019), 10 vom: 12. Juni, Seite 1385-1394 (DE-627)518346137 (DE-600)2252542-7 1336-9563 nnns volume:74 year:2019 number:10 day:12 month:06 pages:1385-1394 https://dx.doi.org/10.2478/s11756-019-00288-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 74 2019 10 12 06 1385-1394 |
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Enthalten in Biologia 74(2019), 10 vom: 12. Juni, Seite 1385-1394 volume:74 year:2019 number:10 day:12 month:06 pages:1385-1394 |
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Panaxadiol Poly (I:C) Inflammatory Transcriptome |
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Yang, Zhou-Xin @@aut@@ Guo, Dong-Yang @@aut@@ Shen, Ling-Zhi @@aut@@ Mao, Gen-Xiang @@aut@@ Dai, Ji-Huan @@aut@@ Chen, Sha-Sha @@aut@@ Yan, Jing @@aut@@ |
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Yang, Zhou-Xin |
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Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol Panaxadiol (dpeaa)DE-He213 Poly (I:C) (dpeaa)DE-He213 Inflammatory (dpeaa)DE-He213 Transcriptome (dpeaa)DE-He213 |
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transcriptome profiling of poly(i:c)-induced raw 264.7 mouse macrophages in response to panaxadiol |
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Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol |
| abstract |
Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. © Institute of Molecular Biology, Slovak Academy of Sciences 2019 |
| abstractGer |
Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. © Institute of Molecular Biology, Slovak Academy of Sciences 2019 |
| abstract_unstemmed |
Abstract Panaxadiol is an active compound in steamed ginseng that possesses anti-cancer properties. Nonetheless, the effect of panaxadiol on inflammation remains unclear. Double-stranded RNA (dsRNA) acts as a molecular mimic associated with viral infection. It is recognized by Toll-like receptor-3 (TLR3), and it induces pro-inflammatory cytokines production of immune cells-like macrophages. Synthetic dsRNA polyriboinosinic polyribocytidylic acid [Poly(I:C)] is structurally similar to double-stranded RNA, which is used to simulate dsRNA in inducing immune response. In this study, we used an RNA-Seq based approach to analyze changes in mRNA level of poly (I:C)-induced RAW264.7 treated by panaxadiol. First, our data suggested that 20 μM and 40 μM panaxadiol may reduce poly(I:C)-induced RAW264.7 cell viability, while the concertation of 10 μM panaxadiol was enough to inhibit the cell production of TNF-α and IL-6. Next, 10 μM panaxadiol was applied to investigate the transcriptome profile change of Poly(I:C)-induced RAW264.7 cells. Transcriptome profile showed that inflammatory related genes (such as Cxcl2, Csf3, Ptgs2, Acod1, Lif and Il1b) could be downregulated by panaxadiol, which was confirmed by real-time PCR. Moreover, Gene Ontology enrichment analysis showed that downregulated genes were associated with immune responses, while many upregulated genes were related to DNA replication. In addition, KEGG enrichment showed changes of pathways like NF-kappa B signaling pathway and DNA replication after treatment with panaxadiol. To sum up, our results suggested that panaxadiol may inhibit inflammatory associated genes in macrophages induced by poly(I:C). Considering its anti-virus effects, panaxadiol could be used to treat a variety of virus related diseases. © Institute of Molecular Biology, Slovak Academy of Sciences 2019 |
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Transcriptome profiling of poly(I:C)-induced RAW 264.7 mouse macrophages in response to panaxadiol |
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Guo, Dong-Yang Shen, Ling-Zhi Mao, Gen-Xiang Dai, Ji-Huan Chen, Sha-Sha Yan, Jing |
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| score |
7.399168 |