The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells
Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bod...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Liu, Junfeng [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Anmerkung: |
© Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
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| Übergeordnetes Werk: |
Enthalten in: Chinese journal of clinical oncology - Beijing : Chinese Anti-Cancer Association, 2004, 6(2009), 1 vom: 30. Mai |
|---|---|
| Übergeordnetes Werk: |
volume:6 ; year:2009 ; number:1 ; day:30 ; month:05 |
| Links: |
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| DOI / URN: |
10.1007/s11805-009-0051-9 |
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| 520 | |a Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. | ||
| 700 | 1 | |a He, Zhixu |4 aut | |
| 700 | 1 | |a Shen, Dong |4 aut | |
| 700 | 1 | |a Huang, Jin |4 aut | |
| 700 | 1 | |a Wang, Haowen |4 aut | |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells |
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The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells |
| abstract |
Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
| abstractGer |
Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
| abstract_unstemmed |
Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
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He, Zhixu Shen, Dong Huang, Jin Wang, Haowen |
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| doi_str |
10.1007/s11805-009-0051-9 |
| up_date |
2024-07-04T03:11:56.386Z |
| _version_ |
1803616482907652096 |
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Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). 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