The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells
Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bod...
Ausführliche Beschreibung
Autor*in: |
Liu, Junfeng [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2009 |
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Anmerkung: |
© Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
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Übergeordnetes Werk: |
Enthalten in: Chinese journal of clinical oncology - Beijing : Chinese Anti-Cancer Association, 2004, 6(2009), 1 vom: 30. Mai |
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Übergeordnetes Werk: |
volume:6 ; year:2009 ; number:1 ; day:30 ; month:05 |
Links: |
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DOI / URN: |
10.1007/s11805-009-0051-9 |
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SPR022477365 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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10.1007/s11805-009-0051-9 doi (DE-627)SPR022477365 (SPR)s11805-009-0051-9-e DE-627 ger DE-627 rakwb eng Liu, Junfeng verfasserin aut The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. He, Zhixu aut Shen, Dong aut Huang, Jin aut Wang, Haowen aut Enthalten in Chinese journal of clinical oncology Beijing : Chinese Anti-Cancer Association, 2004 6(2009), 1 vom: 30. Mai (DE-627)545783674 (DE-600)2388203-7 1993-5145 nnns volume:6 year:2009 number:1 day:30 month:05 https://dx.doi.org/10.1007/s11805-009-0051-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_40 GBV_ILN_702 AR 6 2009 1 30 05 |
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Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
abstractGer |
Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
abstract_unstemmed |
Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine. © Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR022477365</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519074259.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201006s2009 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s11805-009-0051-9</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR022477365</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s11805-009-0051-9-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Liu, Junfeng</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The comparison of biologic characteristics between mice embryonic stem cells and bone marrow derived dendritic cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2009</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Tianjin Medical University Cancer Institute and Hospital and Springer-Verlag GmbH 2009</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objective This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. Methods Embryonic stem cells (ESCs) suspending cultured in petri dishes were induced to generate embryonic bodies (EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented 25 ng/ml GMCSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerous immature DCs outgrown. Meantime, mononuclear cells isolated from mice bone marrow were induced to derive dendritic cells by supplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then the morphology, phenotype and function of both dendritic cells from different origins were examined. Results Growing mature through exposure to lipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited dramatic veils of cytoplasm and extensive dendrites on their surfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reaction (MLR). Conclusion ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumor vaccine.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Zhixu</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Shen, Dong</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Huang, Jin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Haowen</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Chinese journal of clinical oncology</subfield><subfield code="d">Beijing : Chinese Anti-Cancer Association, 2004</subfield><subfield code="g">6(2009), 1 vom: 30. 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