DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective
Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Anantha Narayana, D. B. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Anmerkung: |
© Korean Society for Plant Biotechnology 2019 |
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| Übergeordnetes Werk: |
Enthalten in: Plant biotechnology reports - Tokyo : Springer Japan, 2007, 13(2019), 3 vom: 17. Mai, Seite 201-210 |
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| Übergeordnetes Werk: |
volume:13 ; year:2019 ; number:3 ; day:17 ; month:05 ; pages:201-210 |
| Links: |
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| DOI / URN: |
10.1007/s11816-019-00538-z |
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| Katalog-ID: |
SPR022533524 |
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| 520 | |a Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. | ||
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| 650 | 4 | |a Herbal raw material |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Regulatory status |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Johnson, Sudhakar T. |4 aut | |
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10.1007/s11816-019-00538-z doi (DE-627)SPR022533524 (SPR)s11816-019-00538-z-e DE-627 ger DE-627 rakwb eng Anantha Narayana, D. B. verfasserin (orcid)0000-0003-3381-8127 aut DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society for Plant Biotechnology 2019 Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. DNA barcode (dpeaa)DE-He213 Botanicals authentication (dpeaa)DE-He213 Herbal extracts (dpeaa)DE-He213 Herbal raw material (dpeaa)DE-He213 Regulatory status (dpeaa)DE-He213 Johnson, Sudhakar T. aut Enthalten in Plant biotechnology reports Tokyo : Springer Japan, 2007 13(2019), 3 vom: 17. Mai, Seite 201-210 (DE-627)534054390 (DE-600)2364226-9 1863-5474 nnns volume:13 year:2019 number:3 day:17 month:05 pages:201-210 https://dx.doi.org/10.1007/s11816-019-00538-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2019 3 17 05 201-210 |
| spelling |
10.1007/s11816-019-00538-z doi (DE-627)SPR022533524 (SPR)s11816-019-00538-z-e DE-627 ger DE-627 rakwb eng Anantha Narayana, D. B. verfasserin (orcid)0000-0003-3381-8127 aut DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society for Plant Biotechnology 2019 Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. DNA barcode (dpeaa)DE-He213 Botanicals authentication (dpeaa)DE-He213 Herbal extracts (dpeaa)DE-He213 Herbal raw material (dpeaa)DE-He213 Regulatory status (dpeaa)DE-He213 Johnson, Sudhakar T. aut Enthalten in Plant biotechnology reports Tokyo : Springer Japan, 2007 13(2019), 3 vom: 17. Mai, Seite 201-210 (DE-627)534054390 (DE-600)2364226-9 1863-5474 nnns volume:13 year:2019 number:3 day:17 month:05 pages:201-210 https://dx.doi.org/10.1007/s11816-019-00538-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2019 3 17 05 201-210 |
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10.1007/s11816-019-00538-z doi (DE-627)SPR022533524 (SPR)s11816-019-00538-z-e DE-627 ger DE-627 rakwb eng Anantha Narayana, D. B. verfasserin (orcid)0000-0003-3381-8127 aut DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society for Plant Biotechnology 2019 Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. DNA barcode (dpeaa)DE-He213 Botanicals authentication (dpeaa)DE-He213 Herbal extracts (dpeaa)DE-He213 Herbal raw material (dpeaa)DE-He213 Regulatory status (dpeaa)DE-He213 Johnson, Sudhakar T. aut Enthalten in Plant biotechnology reports Tokyo : Springer Japan, 2007 13(2019), 3 vom: 17. Mai, Seite 201-210 (DE-627)534054390 (DE-600)2364226-9 1863-5474 nnns volume:13 year:2019 number:3 day:17 month:05 pages:201-210 https://dx.doi.org/10.1007/s11816-019-00538-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2019 3 17 05 201-210 |
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10.1007/s11816-019-00538-z doi (DE-627)SPR022533524 (SPR)s11816-019-00538-z-e DE-627 ger DE-627 rakwb eng Anantha Narayana, D. B. verfasserin (orcid)0000-0003-3381-8127 aut DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society for Plant Biotechnology 2019 Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. DNA barcode (dpeaa)DE-He213 Botanicals authentication (dpeaa)DE-He213 Herbal extracts (dpeaa)DE-He213 Herbal raw material (dpeaa)DE-He213 Regulatory status (dpeaa)DE-He213 Johnson, Sudhakar T. aut Enthalten in Plant biotechnology reports Tokyo : Springer Japan, 2007 13(2019), 3 vom: 17. Mai, Seite 201-210 (DE-627)534054390 (DE-600)2364226-9 1863-5474 nnns volume:13 year:2019 number:3 day:17 month:05 pages:201-210 https://dx.doi.org/10.1007/s11816-019-00538-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2019 3 17 05 201-210 |
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10.1007/s11816-019-00538-z doi (DE-627)SPR022533524 (SPR)s11816-019-00538-z-e DE-627 ger DE-627 rakwb eng Anantha Narayana, D. B. verfasserin (orcid)0000-0003-3381-8127 aut DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society for Plant Biotechnology 2019 Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. DNA barcode (dpeaa)DE-He213 Botanicals authentication (dpeaa)DE-He213 Herbal extracts (dpeaa)DE-He213 Herbal raw material (dpeaa)DE-He213 Regulatory status (dpeaa)DE-He213 Johnson, Sudhakar T. aut Enthalten in Plant biotechnology reports Tokyo : Springer Japan, 2007 13(2019), 3 vom: 17. Mai, Seite 201-210 (DE-627)534054390 (DE-600)2364226-9 1863-5474 nnns volume:13 year:2019 number:3 day:17 month:05 pages:201-210 https://dx.doi.org/10.1007/s11816-019-00538-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2019 3 17 05 201-210 |
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Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. 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Anantha Narayana, D. B. |
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Anantha Narayana, D. B. misc DNA barcode misc Botanicals authentication misc Herbal extracts misc Herbal raw material misc Regulatory status DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective |
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DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective DNA barcode (dpeaa)DE-He213 Botanicals authentication (dpeaa)DE-He213 Herbal extracts (dpeaa)DE-He213 Herbal raw material (dpeaa)DE-He213 Regulatory status (dpeaa)DE-He213 |
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DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective |
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dna barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective |
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DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective |
| abstract |
Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. © Korean Society for Plant Biotechnology 2019 |
| abstractGer |
Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. © Korean Society for Plant Biotechnology 2019 |
| abstract_unstemmed |
Abstract Cultivation, supply, and availability of unadulterated plant raw material in botanical or herbal industry are a challenging task. Plant species that are either in short supply or in high demand are prone to adulteration and substitution. Such unscrupulous activity results in availability of substandard products with declining quality standards ultimately posing safety concerns and health of consumers. Raw materials which are in the form of dried, fragmented or powdered forms are major victims of adulteration. We present here DNA-based marker approach to authenticate botanicals. Recent research advances such as 2D-electrophoresis, microarray, next generation sequencing, high-resolution melting analysis, and PCR-based methods have been reviewed and their role in authentication of raw material, botanicals in herbal extracts, finished formulations, and ingredients in dietary supplements has been presented. Due to extraction processes such as steaming, extraction at extreme conditions and presence of secondary metabolites original DNA integrity are not preserved which lead to interference with DNA extraction and PCR amplification, thus making DNA unsuitable for further molecular analysis. There is a scope to develop and validate DNA mini-barcodes for finished formulations. DNA barcode along with chromatographic methods, metabolomics, proteomics, and genomics has to be used to resolve substitution and quantity of raw material in ambiguity. Emerging regulatory scenario on DNA barcode in various Pharmacopoeia is presented. Though several advanced methods of botanical authentication are available, an industry always expects simple, cost efficient, and quick method to resolve authentication issues. © Korean Society for Plant Biotechnology 2019 |
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DNA barcoding in authentication of herbal raw materials, extracts and dietary supplements: a perspective |
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https://dx.doi.org/10.1007/s11816-019-00538-z |
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Johnson, Sudhakar T. |
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10.1007/s11816-019-00538-z |
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2024-07-03T13:30:54.742Z |
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| score |
7.400881 |