Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture
Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultur...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Abushik, P. A. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Anmerkung: |
© Pleiades Publishing, Ltd. 2011 |
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| Übergeordnetes Werk: |
Enthalten in: Biochemistry (Moscow) - Dordrecht [u.a.] : Springer Science + Business Media B.V, 2007, 5(2011), 1 vom: März, Seite 92-100 |
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| Übergeordnetes Werk: |
volume:5 ; year:2011 ; number:1 ; month:03 ; pages:92-100 |
| Links: |
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| DOI / URN: |
10.1134/S1990747810061017 |
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SPR02259289X |
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| 245 | 1 | 0 | |a Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture |
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| 520 | |a Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. | ||
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| 700 | 1 | |a Sibarov, D. A. |4 aut | |
| 700 | 1 | |a Antonov, S. M. |4 aut | |
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10.1134/S1990747810061017 doi (DE-627)SPR02259289X (SPR)S1990747810061017-e DE-627 ger DE-627 rakwb eng Abushik, P. A. verfasserin aut Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2011 Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. kainite (dpeaa)DE-He213 intracellular calcium (dpeaa)DE-He213 neurons (dpeaa)DE-He213 ion channels (dpeaa)DE-He213 glutamate receptors (dpeaa)DE-He213 Bolshakov, A. E. aut Sibarov, D. A. aut Antonov, S. M. aut Enthalten in Biochemistry (Moscow) Dordrecht [u.a.] : Springer Science + Business Media B.V, 2007 5(2011), 1 vom: März, Seite 92-100 (DE-627)546011217 (DE-600)2390072-6 1990-7494 nnns volume:5 year:2011 number:1 month:03 pages:92-100 https://dx.doi.org/10.1134/S1990747810061017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2011 1 03 92-100 |
| spelling |
10.1134/S1990747810061017 doi (DE-627)SPR02259289X (SPR)S1990747810061017-e DE-627 ger DE-627 rakwb eng Abushik, P. A. verfasserin aut Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2011 Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. kainite (dpeaa)DE-He213 intracellular calcium (dpeaa)DE-He213 neurons (dpeaa)DE-He213 ion channels (dpeaa)DE-He213 glutamate receptors (dpeaa)DE-He213 Bolshakov, A. E. aut Sibarov, D. A. aut Antonov, S. M. aut Enthalten in Biochemistry (Moscow) Dordrecht [u.a.] : Springer Science + Business Media B.V, 2007 5(2011), 1 vom: März, Seite 92-100 (DE-627)546011217 (DE-600)2390072-6 1990-7494 nnns volume:5 year:2011 number:1 month:03 pages:92-100 https://dx.doi.org/10.1134/S1990747810061017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2011 1 03 92-100 |
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10.1134/S1990747810061017 doi (DE-627)SPR02259289X (SPR)S1990747810061017-e DE-627 ger DE-627 rakwb eng Abushik, P. A. verfasserin aut Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2011 Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. kainite (dpeaa)DE-He213 intracellular calcium (dpeaa)DE-He213 neurons (dpeaa)DE-He213 ion channels (dpeaa)DE-He213 glutamate receptors (dpeaa)DE-He213 Bolshakov, A. E. aut Sibarov, D. A. aut Antonov, S. M. aut Enthalten in Biochemistry (Moscow) Dordrecht [u.a.] : Springer Science + Business Media B.V, 2007 5(2011), 1 vom: März, Seite 92-100 (DE-627)546011217 (DE-600)2390072-6 1990-7494 nnns volume:5 year:2011 number:1 month:03 pages:92-100 https://dx.doi.org/10.1134/S1990747810061017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2011 1 03 92-100 |
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10.1134/S1990747810061017 doi (DE-627)SPR02259289X (SPR)S1990747810061017-e DE-627 ger DE-627 rakwb eng Abushik, P. A. verfasserin aut Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2011 Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. kainite (dpeaa)DE-He213 intracellular calcium (dpeaa)DE-He213 neurons (dpeaa)DE-He213 ion channels (dpeaa)DE-He213 glutamate receptors (dpeaa)DE-He213 Bolshakov, A. E. aut Sibarov, D. A. aut Antonov, S. M. aut Enthalten in Biochemistry (Moscow) Dordrecht [u.a.] : Springer Science + Business Media B.V, 2007 5(2011), 1 vom: März, Seite 92-100 (DE-627)546011217 (DE-600)2390072-6 1990-7494 nnns volume:5 year:2011 number:1 month:03 pages:92-100 https://dx.doi.org/10.1134/S1990747810061017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2011 1 03 92-100 |
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10.1134/S1990747810061017 doi (DE-627)SPR02259289X (SPR)S1990747810061017-e DE-627 ger DE-627 rakwb eng Abushik, P. A. verfasserin aut Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2011 Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. kainite (dpeaa)DE-He213 intracellular calcium (dpeaa)DE-He213 neurons (dpeaa)DE-He213 ion channels (dpeaa)DE-He213 glutamate receptors (dpeaa)DE-He213 Bolshakov, A. E. aut Sibarov, D. A. aut Antonov, S. M. aut Enthalten in Biochemistry (Moscow) Dordrecht [u.a.] : Springer Science + Business Media B.V, 2007 5(2011), 1 vom: März, Seite 92-100 (DE-627)546011217 (DE-600)2390072-6 1990-7494 nnns volume:5 year:2011 number:1 month:03 pages:92-100 https://dx.doi.org/10.1134/S1990747810061017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2011 1 03 92-100 |
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Enthalten in Biochemistry (Moscow) 5(2011), 1 vom: März, Seite 92-100 volume:5 year:2011 number:1 month:03 pages:92-100 |
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Enthalten in Biochemistry (Moscow) 5(2011), 1 vom: März, Seite 92-100 volume:5 year:2011 number:1 month:03 pages:92-100 |
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Abushik, P. A. @@aut@@ Bolshakov, A. E. @@aut@@ Sibarov, D. A. @@aut@@ Antonov, S. M. @@aut@@ |
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A.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Pleiades Publishing, Ltd. 2011</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. 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Abushik, P. A. |
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Abushik, P. A. misc kainite misc intracellular calcium misc neurons misc ion channels misc glutamate receptors Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture |
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Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture kainite (dpeaa)DE-He213 intracellular calcium (dpeaa)DE-He213 neurons (dpeaa)DE-He213 ion channels (dpeaa)DE-He213 glutamate receptors (dpeaa)DE-He213 |
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misc kainite misc intracellular calcium misc neurons misc ion channels misc glutamate receptors |
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Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture |
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Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture |
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Abushik, P. A. |
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Abushik, P. A. |
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mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture |
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Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture |
| abstract |
Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. © Pleiades Publishing, Ltd. 2011 |
| abstractGer |
Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. © Pleiades Publishing, Ltd. 2011 |
| abstract_unstemmed |
Abstract The dynamics of intracellular $ Ca^{2+} $ signal in response to NMDA (N-methyl-D-aspartate, 30 μM) or KA (kainite, 30 μM), its dependence on extracellular $ Ca^{2+} $ and the mechanisms of KA-triggered $ Ca^{2+} $ entry into neurons have been tested in neurons of rat cortical primary cultures. The level of intracellular free $ Ca^{2+} $ concentrations ([$ Ca^{2+} $]i) was evaluated on Leica SP5 MF confocal microscope using Fluo-3 fluorescent dye, which resolves changes in [$ Ca^{2+} $]i in the micromolar range. The dynamics of [$ Ca^{2+} $]i increase in response to NMDA and KA was different but in both cases the [$ Ca^{2+} $]i increase required the presence of $ Ca^{2+} $ in the extracellular solution. The neuronal population was found to be heterogeneous, based on the response to KA applied together with either L-type calcium channel blocker nifedipine (3 μM) or IEM-1460 (3 μM), a blocker of $ Ca^{2+} $-permeable AMPAR (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) lacking GluR2 subunit. Experiments exhibited three types of calcium responses, characteristically belonging to interneurons (expressing $ Ca^{2+} $-permeable AMPAR), pyramidal neurons (with AMPAR containing GluR2, making them impermeable to $ Ca^{2+} $), and intermediate type of cells expressing both AMPAR types. Thus, we have demonstrated the role of AMPAR and L-type calcium channels in KA-triggered $ Ca^{2+} $ entry into neurons. The dynamics of [$ Ca^{2+} $]i during the KA treatment was shown to depend on subunit composition of particular AMPAR subtype expressed in neurons. The data suggest that neuronal types existing in adult cortical tissue are probably presented in primary culture, too. © Pleiades Publishing, Ltd. 2011 |
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Mechanisms of heterogeneity of calcium response to kainate and neuronal types in rat cortical primary culture |
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https://dx.doi.org/10.1134/S1990747810061017 |
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Bolshakov, A. E. Sibarov, D. A. Antonov, S. M. |
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| score |
7.4019556 |