Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells

Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibili...
Ausführliche Beschreibung

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Autor*in:	Huang, Jin [verfasserIn] Xie, Songlin [verfasserIn] He, Junyao [verfasserIn] Wang, Pu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	Ionic liquids Biodehydrogenation Immobilized cells Growth

Übergeordnetes Werk:	Enthalten in: Applied biochemistry and biotechnology - Berlin : Springer, 1976, 167(2012), 8 vom: 09. Juni, Seite 2131-2143
Übergeordnetes Werk:	volume:167 ; year:2012 ; number:8 ; day:09 ; month:06 ; pages:2131-2143

Links:	Volltext

DOI / URN:	10.1007/s12010-012-9762-0

Katalog-ID:	SPR023565977

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520			\|a Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported.
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700	1		\|a Wang, Pu \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Applied biochemistry and biotechnology \|d Berlin : Springer, 1976 \|g 167(2012), 8 vom: 09. Juni, Seite 2131-2143 \|w (DE-627)342894846 \|w (DE-600)2072711-2 \|x 1559-0291 \|7 nnns
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912			\|a GBV_ILN_70
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912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
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912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
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912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
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allfields	10.1007/s12010-012-9762-0 doi (DE-627)SPR023565977 (SPR)s12010-012-9762-0-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Huang, Jin verfasserin aut Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported. Ionic liquids (dpeaa)DE-He213 Biodehydrogenation (dpeaa)DE-He213 Immobilized cells (dpeaa)DE-He213 Growth (dpeaa)DE-He213 Xie, Songlin verfasserin aut He, Junyao verfasserin aut Wang, Pu verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 167(2012), 8 vom: 09. Juni, Seite 2131-2143 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:167 year:2012 number:8 day:09 month:06 pages:2131-2143 https://dx.doi.org/10.1007/s12010-012-9762-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 167 2012 8 09 06 2131-2143
spelling	10.1007/s12010-012-9762-0 doi (DE-627)SPR023565977 (SPR)s12010-012-9762-0-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Huang, Jin verfasserin aut Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported. Ionic liquids (dpeaa)DE-He213 Biodehydrogenation (dpeaa)DE-He213 Immobilized cells (dpeaa)DE-He213 Growth (dpeaa)DE-He213 Xie, Songlin verfasserin aut He, Junyao verfasserin aut Wang, Pu verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 167(2012), 8 vom: 09. Juni, Seite 2131-2143 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:167 year:2012 number:8 day:09 month:06 pages:2131-2143 https://dx.doi.org/10.1007/s12010-012-9762-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 167 2012 8 09 06 2131-2143
allfields_unstemmed	10.1007/s12010-012-9762-0 doi (DE-627)SPR023565977 (SPR)s12010-012-9762-0-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Huang, Jin verfasserin aut Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported. Ionic liquids (dpeaa)DE-He213 Biodehydrogenation (dpeaa)DE-He213 Immobilized cells (dpeaa)DE-He213 Growth (dpeaa)DE-He213 Xie, Songlin verfasserin aut He, Junyao verfasserin aut Wang, Pu verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 167(2012), 8 vom: 09. Juni, Seite 2131-2143 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:167 year:2012 number:8 day:09 month:06 pages:2131-2143 https://dx.doi.org/10.1007/s12010-012-9762-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 167 2012 8 09 06 2131-2143
allfieldsGer	10.1007/s12010-012-9762-0 doi (DE-627)SPR023565977 (SPR)s12010-012-9762-0-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Huang, Jin verfasserin aut Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported. Ionic liquids (dpeaa)DE-He213 Biodehydrogenation (dpeaa)DE-He213 Immobilized cells (dpeaa)DE-He213 Growth (dpeaa)DE-He213 Xie, Songlin verfasserin aut He, Junyao verfasserin aut Wang, Pu verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 167(2012), 8 vom: 09. Juni, Seite 2131-2143 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:167 year:2012 number:8 day:09 month:06 pages:2131-2143 https://dx.doi.org/10.1007/s12010-012-9762-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 167 2012 8 09 06 2131-2143
allfieldsSound	10.1007/s12010-012-9762-0 doi (DE-627)SPR023565977 (SPR)s12010-012-9762-0-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Huang, Jin verfasserin aut Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported. Ionic liquids (dpeaa)DE-He213 Biodehydrogenation (dpeaa)DE-He213 Immobilized cells (dpeaa)DE-He213 Growth (dpeaa)DE-He213 Xie, Songlin verfasserin aut He, Junyao verfasserin aut Wang, Pu verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 167(2012), 8 vom: 09. Juni, Seite 2131-2143 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:167 year:2012 number:8 day:09 month:06 pages:2131-2143 https://dx.doi.org/10.1007/s12010-012-9762-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 167 2012 8 09 06 2131-2143
language	English
source	Enthalten in Applied biochemistry and biotechnology 167(2012), 8 vom: 09. Juni, Seite 2131-2143 volume:167 year:2012 number:8 day:09 month:06 pages:2131-2143
sourceStr	Enthalten in Applied biochemistry and biotechnology 167(2012), 8 vom: 09. Juni, Seite 2131-2143 volume:167 year:2012 number:8 day:09 month:06 pages:2131-2143
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Ionic liquids Biodehydrogenation Immobilized cells Growth
dewey-raw	570
isfreeaccess_bool	false
container_title	Applied biochemistry and biotechnology
authorswithroles_txt_mv	Huang, Jin @@aut@@ Xie, Songlin @@aut@@ He, Junyao @@aut@@ Wang, Pu @@aut@@
publishDateDaySort_date	2012-06-09T00:00:00Z
hierarchy_top_id	342894846
dewey-sort	3570
id	SPR023565977
language_de	englisch
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author	Huang, Jin
spellingShingle	Huang, Jin ddc 570 ddc 540 misc Ionic liquids misc Biodehydrogenation misc Immobilized cells misc Growth Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells
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topic_title	570 660 ASE 540 660 ASE Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells Ionic liquids (dpeaa)DE-He213 Biodehydrogenation (dpeaa)DE-He213 Immobilized cells (dpeaa)DE-He213 Growth (dpeaa)DE-He213
topic	ddc 570 ddc 540 misc Ionic liquids misc Biodehydrogenation misc Immobilized cells misc Growth
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title	Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells
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title_full	Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells
author_sort	Huang, Jin
journal	Applied biochemistry and biotechnology
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author_browse	Huang, Jin Xie, Songlin He, Junyao Wang, Pu
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title_sort	effects of ionic liquids on the growth of arthrobacter simplex and improved biodehydrogenation in an ionic liquid-containing system with immobilized cells
title_auth	Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells
abstract	Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported.
abstractGer	Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported.
abstract_unstemmed	Abstract Dehydrogenated derivatives of corticosteroids are usually more effective than their precursors in treating diseases. In this study, the toxicity of seven water-miscible ionic liquid and three organic solvents to the biocatalyst Arthrobacter simplex UR016 was tested to evaluate the possibility of biodehydrogenation from 17α-hydroxy-16β-methyl-pregna-4,9(11)-diene-3,20-dione (HMPDD) to 17α-hydroxy-16β-methyl-pregna-1,4,9(11)-triene-3,20-dione (HMPTD) in an ionic liquid-containing system. Although most tested room temperature ionic liquids (RTILs) showed higher toxicities to A. simplex UR016 than organic solvents, bacterial growth was promoted in the presence of [EMIM](l)-Lac or [BMIM](l)-Lac at concentrations below 2.5 mmol/l, especially [EMIM](l)-Lac, presented the lowest toxicity to A. simplex. Following immobilization investigations, a conversion ratio of 89.9 % was achieved with a cell biomass of 10 g/l (dry cell weight/reaction mixture volume) in the polyethylene glycol (PEG)-modified calcium alginate gel bead, a suitable matrix for cell immobilization. Further studies indicated that the conversion ratio can be improved by increasing cell loading to 60 beads per flask (containing 30 ml reaction mixture). Under optimal conditions with a [EMIM](l)-Lac content of 0.3 %, the conversion ratio reached 93.4 %, the highest value ever reported.
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title_short	Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells
url	https://dx.doi.org/10.1007/s12010-012-9762-0
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up_date	2024-07-03T19:44:56.566Z
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Effects of Ionic Liquids on the Growth of Arthrobacter simplex and Improved Biodehydrogenation in an Ionic Liquid-Containing System with Immobilized Cells

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