Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection
Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (C...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Muenchen, Daniela Kunkel [verfasserIn] Martinazzo, Janine [verfasserIn] Brezolin, Alexandra Nava [verfasserIn] de Cezaro, Alana Marie [verfasserIn] Rigo, Aline Andressa [verfasserIn] Mezarroba, Mateus Nava [verfasserIn] Manzoli, Alexandra [verfasserIn] de Lima Leite, Fábio [verfasserIn] Steffens, Juliana [verfasserIn] Steffens, Clarice [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Applied biochemistry and biotechnology - Berlin : Springer, 1976, 186(2018), 4 vom: 04. Juni, Seite 1061-1073 |
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| Übergeordnetes Werk: |
volume:186 ; year:2018 ; number:4 ; day:04 ; month:06 ; pages:1061-1073 |
| Links: |
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| DOI / URN: |
10.1007/s12010-018-2799-y |
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| Katalog-ID: |
SPR023599448 |
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| 520 | |a Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. | ||
| 650 | 4 | |a Zucchini |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Enzymatic activity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Functionalization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Self-assembled monolayers (SAM) |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Nanobiosensor |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Atomic force microscopy (AFM) |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Martinazzo, Janine |e verfasserin |4 aut | |
| 700 | 1 | |a Brezolin, Alexandra Nava |e verfasserin |4 aut | |
| 700 | 1 | |a de Cezaro, Alana Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Rigo, Aline Andressa |e verfasserin |4 aut | |
| 700 | 1 | |a Mezarroba, Mateus Nava |e verfasserin |4 aut | |
| 700 | 1 | |a Manzoli, Alexandra |e verfasserin |4 aut | |
| 700 | 1 | |a de Lima Leite, Fábio |e verfasserin |4 aut | |
| 700 | 1 | |a Steffens, Juliana |e verfasserin |4 aut | |
| 700 | 1 | |a Steffens, Clarice |e verfasserin |4 aut | |
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10.1007/s12010-018-2799-y doi (DE-627)SPR023599448 (SPR)s12010-018-2799-y-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Muenchen, Daniela Kunkel verfasserin aut Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. Zucchini (dpeaa)DE-He213 Enzymatic activity (dpeaa)DE-He213 Functionalization (dpeaa)DE-He213 Self-assembled monolayers (SAM) (dpeaa)DE-He213 Nanobiosensor (dpeaa)DE-He213 Atomic force microscopy (AFM) (dpeaa)DE-He213 Martinazzo, Janine verfasserin aut Brezolin, Alexandra Nava verfasserin aut de Cezaro, Alana Marie verfasserin aut Rigo, Aline Andressa verfasserin aut Mezarroba, Mateus Nava verfasserin aut Manzoli, Alexandra verfasserin aut de Lima Leite, Fábio verfasserin aut Steffens, Juliana verfasserin aut Steffens, Clarice verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 186(2018), 4 vom: 04. Juni, Seite 1061-1073 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:186 year:2018 number:4 day:04 month:06 pages:1061-1073 https://dx.doi.org/10.1007/s12010-018-2799-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 186 2018 4 04 06 1061-1073 |
| spelling |
10.1007/s12010-018-2799-y doi (DE-627)SPR023599448 (SPR)s12010-018-2799-y-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Muenchen, Daniela Kunkel verfasserin aut Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. Zucchini (dpeaa)DE-He213 Enzymatic activity (dpeaa)DE-He213 Functionalization (dpeaa)DE-He213 Self-assembled monolayers (SAM) (dpeaa)DE-He213 Nanobiosensor (dpeaa)DE-He213 Atomic force microscopy (AFM) (dpeaa)DE-He213 Martinazzo, Janine verfasserin aut Brezolin, Alexandra Nava verfasserin aut de Cezaro, Alana Marie verfasserin aut Rigo, Aline Andressa verfasserin aut Mezarroba, Mateus Nava verfasserin aut Manzoli, Alexandra verfasserin aut de Lima Leite, Fábio verfasserin aut Steffens, Juliana verfasserin aut Steffens, Clarice verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 186(2018), 4 vom: 04. Juni, Seite 1061-1073 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:186 year:2018 number:4 day:04 month:06 pages:1061-1073 https://dx.doi.org/10.1007/s12010-018-2799-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 186 2018 4 04 06 1061-1073 |
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10.1007/s12010-018-2799-y doi (DE-627)SPR023599448 (SPR)s12010-018-2799-y-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Muenchen, Daniela Kunkel verfasserin aut Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. Zucchini (dpeaa)DE-He213 Enzymatic activity (dpeaa)DE-He213 Functionalization (dpeaa)DE-He213 Self-assembled monolayers (SAM) (dpeaa)DE-He213 Nanobiosensor (dpeaa)DE-He213 Atomic force microscopy (AFM) (dpeaa)DE-He213 Martinazzo, Janine verfasserin aut Brezolin, Alexandra Nava verfasserin aut de Cezaro, Alana Marie verfasserin aut Rigo, Aline Andressa verfasserin aut Mezarroba, Mateus Nava verfasserin aut Manzoli, Alexandra verfasserin aut de Lima Leite, Fábio verfasserin aut Steffens, Juliana verfasserin aut Steffens, Clarice verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 186(2018), 4 vom: 04. Juni, Seite 1061-1073 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:186 year:2018 number:4 day:04 month:06 pages:1061-1073 https://dx.doi.org/10.1007/s12010-018-2799-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 186 2018 4 04 06 1061-1073 |
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10.1007/s12010-018-2799-y doi (DE-627)SPR023599448 (SPR)s12010-018-2799-y-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Muenchen, Daniela Kunkel verfasserin aut Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. Zucchini (dpeaa)DE-He213 Enzymatic activity (dpeaa)DE-He213 Functionalization (dpeaa)DE-He213 Self-assembled monolayers (SAM) (dpeaa)DE-He213 Nanobiosensor (dpeaa)DE-He213 Atomic force microscopy (AFM) (dpeaa)DE-He213 Martinazzo, Janine verfasserin aut Brezolin, Alexandra Nava verfasserin aut de Cezaro, Alana Marie verfasserin aut Rigo, Aline Andressa verfasserin aut Mezarroba, Mateus Nava verfasserin aut Manzoli, Alexandra verfasserin aut de Lima Leite, Fábio verfasserin aut Steffens, Juliana verfasserin aut Steffens, Clarice verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 186(2018), 4 vom: 04. Juni, Seite 1061-1073 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:186 year:2018 number:4 day:04 month:06 pages:1061-1073 https://dx.doi.org/10.1007/s12010-018-2799-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 186 2018 4 04 06 1061-1073 |
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10.1007/s12010-018-2799-y doi (DE-627)SPR023599448 (SPR)s12010-018-2799-y-e DE-627 ger DE-627 rakwb eng 570 660 ASE 540 660 ASE Muenchen, Daniela Kunkel verfasserin aut Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. Zucchini (dpeaa)DE-He213 Enzymatic activity (dpeaa)DE-He213 Functionalization (dpeaa)DE-He213 Self-assembled monolayers (SAM) (dpeaa)DE-He213 Nanobiosensor (dpeaa)DE-He213 Atomic force microscopy (AFM) (dpeaa)DE-He213 Martinazzo, Janine verfasserin aut Brezolin, Alexandra Nava verfasserin aut de Cezaro, Alana Marie verfasserin aut Rigo, Aline Andressa verfasserin aut Mezarroba, Mateus Nava verfasserin aut Manzoli, Alexandra verfasserin aut de Lima Leite, Fábio verfasserin aut Steffens, Juliana verfasserin aut Steffens, Clarice verfasserin aut Enthalten in Applied biochemistry and biotechnology Berlin : Springer, 1976 186(2018), 4 vom: 04. Juni, Seite 1061-1073 (DE-627)342894846 (DE-600)2072711-2 1559-0291 nnns volume:186 year:2018 number:4 day:04 month:06 pages:1061-1073 https://dx.doi.org/10.1007/s12010-018-2799-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 186 2018 4 04 06 1061-1073 |
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Enthalten in Applied biochemistry and biotechnology 186(2018), 4 vom: 04. Juni, Seite 1061-1073 volume:186 year:2018 number:4 day:04 month:06 pages:1061-1073 |
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Enthalten in Applied biochemistry and biotechnology 186(2018), 4 vom: 04. Juni, Seite 1061-1073 volume:186 year:2018 number:4 day:04 month:06 pages:1061-1073 |
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Zucchini Enzymatic activity Functionalization Self-assembled monolayers (SAM) Nanobiosensor Atomic force microscopy (AFM) |
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Muenchen, Daniela Kunkel @@aut@@ Martinazzo, Janine @@aut@@ Brezolin, Alexandra Nava @@aut@@ de Cezaro, Alana Marie @@aut@@ Rigo, Aline Andressa @@aut@@ Mezarroba, Mateus Nava @@aut@@ Manzoli, Alexandra @@aut@@ de Lima Leite, Fábio @@aut@@ Steffens, Juliana @@aut@@ Steffens, Clarice @@aut@@ |
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The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. 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Muenchen, Daniela Kunkel Martinazzo, Janine Brezolin, Alexandra Nava de Cezaro, Alana Marie Rigo, Aline Andressa Mezarroba, Mateus Nava Manzoli, Alexandra de Lima Leite, Fábio Steffens, Juliana Steffens, Clarice |
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Muenchen, Daniela Kunkel |
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cantilever functionalization using peroxidase extract of low cost for glyphosate detection |
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Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection |
| abstract |
Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. |
| abstractGer |
Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. |
| abstract_unstemmed |
Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg $ L^{−1} $) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg $ L^{−1} $. |
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Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection |
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Martinazzo, Janine Brezolin, Alexandra Nava de Cezaro, Alana Marie Rigo, Aline Andressa Mezarroba, Mateus Nava Manzoli, Alexandra de Lima Leite, Fábio Steffens, Juliana Steffens, Clarice |
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| score |
7.4000187 |