Alkaline Phosphatase Expression/Activity and Multilineage Differentiation Potential are the Differences Between Fibroblasts and Orbital Fat-Derived Stem Cells – A Study in Animal Serum-Free Culture Conditions
Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Martins, Thaís Maria da Mata [verfasserIn] de Paula, Ana Cláudia Chagas [verfasserIn] Gomes, Dawidson Assis [verfasserIn] Goes, Alfredo Miranda [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Stem cell reviews - New York, NY : Springer, 2005, 10(2014), 5 vom: 10. Juni, Seite 697-711 |
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| Übergeordnetes Werk: |
volume:10 ; year:2014 ; number:5 ; day:10 ; month:06 ; pages:697-711 |
| Links: |
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| DOI / URN: |
10.1007/s12015-014-9529-9 |
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| 520 | |a Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. | ||
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10.1007/s12015-014-9529-9 doi (DE-627)SPR023688556 (SPR)s12015-014-9529-9-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.15 bkl Martins, Thaís Maria da Mata verfasserin aut Alkaline Phosphatase Expression/Activity and Multilineage Differentiation Potential are the Differences Between Fibroblasts and Orbital Fat-Derived Stem Cells – A Study in Animal Serum-Free Culture Conditions 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. Cell culture medium (dpeaa)DE-He213 Human serum (dpeaa)DE-He213 Orbital fat-derived stem cells (dpeaa)DE-He213 Fibroblasts (dpeaa)DE-He213 Differentiation (dpeaa)DE-He213 Alkaline phosphatase (dpeaa)DE-He213 de Paula, Ana Cláudia Chagas verfasserin aut Gomes, Dawidson Assis verfasserin aut Goes, Alfredo Miranda verfasserin aut Enthalten in Stem cell reviews New York, NY : Springer, 2005 10(2014), 5 vom: 10. Juni, Seite 697-711 (DE-627)494833777 (DE-600)2197218-7 1558-6804 nnns volume:10 year:2014 number:5 day:10 month:06 pages:697-711 https://dx.doi.org/10.1007/s12015-014-9529-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 42.15 ASE AR 10 2014 5 10 06 697-711 |
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10.1007/s12015-014-9529-9 doi (DE-627)SPR023688556 (SPR)s12015-014-9529-9-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.15 bkl Martins, Thaís Maria da Mata verfasserin aut Alkaline Phosphatase Expression/Activity and Multilineage Differentiation Potential are the Differences Between Fibroblasts and Orbital Fat-Derived Stem Cells – A Study in Animal Serum-Free Culture Conditions 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. Cell culture medium (dpeaa)DE-He213 Human serum (dpeaa)DE-He213 Orbital fat-derived stem cells (dpeaa)DE-He213 Fibroblasts (dpeaa)DE-He213 Differentiation (dpeaa)DE-He213 Alkaline phosphatase (dpeaa)DE-He213 de Paula, Ana Cláudia Chagas verfasserin aut Gomes, Dawidson Assis verfasserin aut Goes, Alfredo Miranda verfasserin aut Enthalten in Stem cell reviews New York, NY : Springer, 2005 10(2014), 5 vom: 10. Juni, Seite 697-711 (DE-627)494833777 (DE-600)2197218-7 1558-6804 nnns volume:10 year:2014 number:5 day:10 month:06 pages:697-711 https://dx.doi.org/10.1007/s12015-014-9529-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 42.15 ASE AR 10 2014 5 10 06 697-711 |
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10.1007/s12015-014-9529-9 doi (DE-627)SPR023688556 (SPR)s12015-014-9529-9-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.15 bkl Martins, Thaís Maria da Mata verfasserin aut Alkaline Phosphatase Expression/Activity and Multilineage Differentiation Potential are the Differences Between Fibroblasts and Orbital Fat-Derived Stem Cells – A Study in Animal Serum-Free Culture Conditions 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. Cell culture medium (dpeaa)DE-He213 Human serum (dpeaa)DE-He213 Orbital fat-derived stem cells (dpeaa)DE-He213 Fibroblasts (dpeaa)DE-He213 Differentiation (dpeaa)DE-He213 Alkaline phosphatase (dpeaa)DE-He213 de Paula, Ana Cláudia Chagas verfasserin aut Gomes, Dawidson Assis verfasserin aut Goes, Alfredo Miranda verfasserin aut Enthalten in Stem cell reviews New York, NY : Springer, 2005 10(2014), 5 vom: 10. Juni, Seite 697-711 (DE-627)494833777 (DE-600)2197218-7 1558-6804 nnns volume:10 year:2014 number:5 day:10 month:06 pages:697-711 https://dx.doi.org/10.1007/s12015-014-9529-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 42.15 ASE AR 10 2014 5 10 06 697-711 |
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10.1007/s12015-014-9529-9 doi (DE-627)SPR023688556 (SPR)s12015-014-9529-9-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.15 bkl Martins, Thaís Maria da Mata verfasserin aut Alkaline Phosphatase Expression/Activity and Multilineage Differentiation Potential are the Differences Between Fibroblasts and Orbital Fat-Derived Stem Cells – A Study in Animal Serum-Free Culture Conditions 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. Cell culture medium (dpeaa)DE-He213 Human serum (dpeaa)DE-He213 Orbital fat-derived stem cells (dpeaa)DE-He213 Fibroblasts (dpeaa)DE-He213 Differentiation (dpeaa)DE-He213 Alkaline phosphatase (dpeaa)DE-He213 de Paula, Ana Cláudia Chagas verfasserin aut Gomes, Dawidson Assis verfasserin aut Goes, Alfredo Miranda verfasserin aut Enthalten in Stem cell reviews New York, NY : Springer, 2005 10(2014), 5 vom: 10. Juni, Seite 697-711 (DE-627)494833777 (DE-600)2197218-7 1558-6804 nnns volume:10 year:2014 number:5 day:10 month:06 pages:697-711 https://dx.doi.org/10.1007/s12015-014-9529-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 42.15 ASE AR 10 2014 5 10 06 697-711 |
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10.1007/s12015-014-9529-9 doi (DE-627)SPR023688556 (SPR)s12015-014-9529-9-e DE-627 ger DE-627 rakwb eng 570 610 ASE 42.15 bkl Martins, Thaís Maria da Mata verfasserin aut Alkaline Phosphatase Expression/Activity and Multilineage Differentiation Potential are the Differences Between Fibroblasts and Orbital Fat-Derived Stem Cells – A Study in Animal Serum-Free Culture Conditions 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. Cell culture medium (dpeaa)DE-He213 Human serum (dpeaa)DE-He213 Orbital fat-derived stem cells (dpeaa)DE-He213 Fibroblasts (dpeaa)DE-He213 Differentiation (dpeaa)DE-He213 Alkaline phosphatase (dpeaa)DE-He213 de Paula, Ana Cláudia Chagas verfasserin aut Gomes, Dawidson Assis verfasserin aut Goes, Alfredo Miranda verfasserin aut Enthalten in Stem cell reviews New York, NY : Springer, 2005 10(2014), 5 vom: 10. Juni, Seite 697-711 (DE-627)494833777 (DE-600)2197218-7 1558-6804 nnns volume:10 year:2014 number:5 day:10 month:06 pages:697-711 https://dx.doi.org/10.1007/s12015-014-9529-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 42.15 ASE AR 10 2014 5 10 06 697-711 |
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Alkaline Phosphatase Expression/Activity and Multilineage Differentiation Potential are the Differences Between Fibroblasts and Orbital Fat-Derived Stem Cells – A Study in Animal Serum-Free Culture Conditions |
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Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. |
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Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. |
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Abstract Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications. |
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