Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation
Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymph...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Blaszkowska, Joanna [verfasserIn] Bratkowska, Wanda [verfasserIn] Lopaczynska, Dobroslawa [verfasserIn] Ferenc, Tomasz [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of Genetics - Springer India, 1955, 88(2009), 1 vom: 01. Apr. |
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| Übergeordnetes Werk: |
volume:88 ; year:2009 ; number:1 ; day:01 ; month:04 |
| Links: |
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| DOI / URN: |
10.1007/s12041-009-0009-y |
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SPR024083011 |
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| 520 | |a Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. | ||
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| 650 | 4 | |a trypsin inhibitor |7 (dpeaa)DE-He213 | |
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10.1007/s12041-009-0009-y doi (DE-627)SPR024083011 (SPR)s12041-009-0009-y-e DE-627 ger DE-627 rakwb eng Blaszkowska, Joanna verfasserin aut Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. chromosome aberrations (dpeaa)DE-He213 mitotic index (dpeaa)DE-He213 replication index (dpeaa)DE-He213 sister chromatid exchanges (dpeaa)DE-He213 trypsin inhibitor (dpeaa)DE-He213 Bratkowska, Wanda verfasserin aut Lopaczynska, Dobroslawa verfasserin aut Ferenc, Tomasz verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 1 vom: 01. Apr. (DE-627)SPR024069582 nnns volume:88 year:2009 number:1 day:01 month:04 https://dx.doi.org/10.1007/s12041-009-0009-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 1 01 04 |
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10.1007/s12041-009-0009-y doi (DE-627)SPR024083011 (SPR)s12041-009-0009-y-e DE-627 ger DE-627 rakwb eng Blaszkowska, Joanna verfasserin aut Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. chromosome aberrations (dpeaa)DE-He213 mitotic index (dpeaa)DE-He213 replication index (dpeaa)DE-He213 sister chromatid exchanges (dpeaa)DE-He213 trypsin inhibitor (dpeaa)DE-He213 Bratkowska, Wanda verfasserin aut Lopaczynska, Dobroslawa verfasserin aut Ferenc, Tomasz verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 1 vom: 01. Apr. (DE-627)SPR024069582 nnns volume:88 year:2009 number:1 day:01 month:04 https://dx.doi.org/10.1007/s12041-009-0009-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 1 01 04 |
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10.1007/s12041-009-0009-y doi (DE-627)SPR024083011 (SPR)s12041-009-0009-y-e DE-627 ger DE-627 rakwb eng Blaszkowska, Joanna verfasserin aut Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. chromosome aberrations (dpeaa)DE-He213 mitotic index (dpeaa)DE-He213 replication index (dpeaa)DE-He213 sister chromatid exchanges (dpeaa)DE-He213 trypsin inhibitor (dpeaa)DE-He213 Bratkowska, Wanda verfasserin aut Lopaczynska, Dobroslawa verfasserin aut Ferenc, Tomasz verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 1 vom: 01. Apr. (DE-627)SPR024069582 nnns volume:88 year:2009 number:1 day:01 month:04 https://dx.doi.org/10.1007/s12041-009-0009-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 1 01 04 |
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10.1007/s12041-009-0009-y doi (DE-627)SPR024083011 (SPR)s12041-009-0009-y-e DE-627 ger DE-627 rakwb eng Blaszkowska, Joanna verfasserin aut Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. chromosome aberrations (dpeaa)DE-He213 mitotic index (dpeaa)DE-He213 replication index (dpeaa)DE-He213 sister chromatid exchanges (dpeaa)DE-He213 trypsin inhibitor (dpeaa)DE-He213 Bratkowska, Wanda verfasserin aut Lopaczynska, Dobroslawa verfasserin aut Ferenc, Tomasz verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 1 vom: 01. Apr. (DE-627)SPR024069582 nnns volume:88 year:2009 number:1 day:01 month:04 https://dx.doi.org/10.1007/s12041-009-0009-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 1 01 04 |
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10.1007/s12041-009-0009-y doi (DE-627)SPR024083011 (SPR)s12041-009-0009-y-e DE-627 ger DE-627 rakwb eng Blaszkowska, Joanna verfasserin aut Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. chromosome aberrations (dpeaa)DE-He213 mitotic index (dpeaa)DE-He213 replication index (dpeaa)DE-He213 sister chromatid exchanges (dpeaa)DE-He213 trypsin inhibitor (dpeaa)DE-He213 Bratkowska, Wanda verfasserin aut Lopaczynska, Dobroslawa verfasserin aut Ferenc, Tomasz verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 1 vom: 01. Apr. (DE-627)SPR024069582 nnns volume:88 year:2009 number:1 day:01 month:04 https://dx.doi.org/10.1007/s12041-009-0009-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 1 01 04 |
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Enthalten in Journal of Genetics 88(2009), 1 vom: 01. Apr. volume:88 year:2009 number:1 day:01 month:04 |
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Enthalten in Journal of Genetics 88(2009), 1 vom: 01. Apr. volume:88 year:2009 number:1 day:01 month:04 |
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Blaszkowska, Joanna @@aut@@ Bratkowska, Wanda @@aut@@ Lopaczynska, Dobroslawa @@aut@@ Ferenc, Tomasz @@aut@@ |
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cytogenetic study of ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation |
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Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation |
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Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. |
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Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. |
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Abstract The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. |
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