Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker
Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Singh, Mamta [verfasserIn] Kumar, Ravindra [verfasserIn] Nagpure, N. S. [verfasserIn] Kushwaha, Basdeo [verfasserIn] Mani, Indra [verfasserIn] Chauhan, U. K. [verfasserIn] Lakra, W. S. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of Genetics - Springer India, 1955, 88(2009), 3 vom: 18. Nov., Seite 315-320 |
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| Übergeordnetes Werk: |
volume:88 ; year:2009 ; number:3 ; day:18 ; month:11 ; pages:315-320 |
| Links: |
|---|
| DOI / URN: |
10.1007/s12041-009-0045-7 |
|---|
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SPR024085790 |
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| 520 | |a Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. | ||
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| 700 | 1 | |a Kushwaha, Basdeo |e verfasserin |4 aut | |
| 700 | 1 | |a Mani, Indra |e verfasserin |4 aut | |
| 700 | 1 | |a Chauhan, U. K. |e verfasserin |4 aut | |
| 700 | 1 | |a Lakra, W. S. |e verfasserin |4 aut | |
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10.1007/s12041-009-0045-7 doi (DE-627)SPR024085790 (SPR)s12041-009-0045-7-e DE-627 ger DE-627 rakwb eng Singh, Mamta verfasserin aut Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. 18S rDNA (dpeaa)DE-He213 5S rDNA (dpeaa)DE-He213 FISH (dpeaa)DE-He213 rDNA (dpeaa)DE-He213 Kumar, Ravindra verfasserin aut Nagpure, N. S. verfasserin aut Kushwaha, Basdeo verfasserin aut Mani, Indra verfasserin aut Chauhan, U. K. verfasserin aut Lakra, W. S. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 3 vom: 18. Nov., Seite 315-320 (DE-627)SPR024069582 nnns volume:88 year:2009 number:3 day:18 month:11 pages:315-320 https://dx.doi.org/10.1007/s12041-009-0045-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 3 18 11 315-320 |
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10.1007/s12041-009-0045-7 doi (DE-627)SPR024085790 (SPR)s12041-009-0045-7-e DE-627 ger DE-627 rakwb eng Singh, Mamta verfasserin aut Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. 18S rDNA (dpeaa)DE-He213 5S rDNA (dpeaa)DE-He213 FISH (dpeaa)DE-He213 rDNA (dpeaa)DE-He213 Kumar, Ravindra verfasserin aut Nagpure, N. S. verfasserin aut Kushwaha, Basdeo verfasserin aut Mani, Indra verfasserin aut Chauhan, U. K. verfasserin aut Lakra, W. S. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 3 vom: 18. Nov., Seite 315-320 (DE-627)SPR024069582 nnns volume:88 year:2009 number:3 day:18 month:11 pages:315-320 https://dx.doi.org/10.1007/s12041-009-0045-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 3 18 11 315-320 |
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10.1007/s12041-009-0045-7 doi (DE-627)SPR024085790 (SPR)s12041-009-0045-7-e DE-627 ger DE-627 rakwb eng Singh, Mamta verfasserin aut Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. 18S rDNA (dpeaa)DE-He213 5S rDNA (dpeaa)DE-He213 FISH (dpeaa)DE-He213 rDNA (dpeaa)DE-He213 Kumar, Ravindra verfasserin aut Nagpure, N. S. verfasserin aut Kushwaha, Basdeo verfasserin aut Mani, Indra verfasserin aut Chauhan, U. K. verfasserin aut Lakra, W. S. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 3 vom: 18. Nov., Seite 315-320 (DE-627)SPR024069582 nnns volume:88 year:2009 number:3 day:18 month:11 pages:315-320 https://dx.doi.org/10.1007/s12041-009-0045-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 3 18 11 315-320 |
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10.1007/s12041-009-0045-7 doi (DE-627)SPR024085790 (SPR)s12041-009-0045-7-e DE-627 ger DE-627 rakwb eng Singh, Mamta verfasserin aut Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. 18S rDNA (dpeaa)DE-He213 5S rDNA (dpeaa)DE-He213 FISH (dpeaa)DE-He213 rDNA (dpeaa)DE-He213 Kumar, Ravindra verfasserin aut Nagpure, N. S. verfasserin aut Kushwaha, Basdeo verfasserin aut Mani, Indra verfasserin aut Chauhan, U. K. verfasserin aut Lakra, W. S. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 3 vom: 18. Nov., Seite 315-320 (DE-627)SPR024069582 nnns volume:88 year:2009 number:3 day:18 month:11 pages:315-320 https://dx.doi.org/10.1007/s12041-009-0045-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 3 18 11 315-320 |
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10.1007/s12041-009-0045-7 doi (DE-627)SPR024085790 (SPR)s12041-009-0045-7-e DE-627 ger DE-627 rakwb eng Singh, Mamta verfasserin aut Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. 18S rDNA (dpeaa)DE-He213 5S rDNA (dpeaa)DE-He213 FISH (dpeaa)DE-He213 rDNA (dpeaa)DE-He213 Kumar, Ravindra verfasserin aut Nagpure, N. S. verfasserin aut Kushwaha, Basdeo verfasserin aut Mani, Indra verfasserin aut Chauhan, U. K. verfasserin aut Lakra, W. S. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 88(2009), 3 vom: 18. Nov., Seite 315-320 (DE-627)SPR024069582 nnns volume:88 year:2009 number:3 day:18 month:11 pages:315-320 https://dx.doi.org/10.1007/s12041-009-0045-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 88 2009 3 18 11 315-320 |
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population distribution of 45s and 5s rdna in golden mahseer, tor putitora: population-specific fish marker |
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Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: population-specific FISH marker |
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Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. |
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Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. |
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Abstract Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and organization of 5S rRNA genes were analysed in five different populations of golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) and Southern blot hybridization. All five populations of T. putitora (2n = 100) showed a similar type of macro-karyotype composed of 12 metacentric, 22 submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of active nucleolar organizer regions (NORs) by silver staining did not show any differences in number and chromosomal position in different populations. But FISH data showed significant difference between the populations, four of the five populations showed six 18S (three pairs) and two 5S (one pair) signals with positional polymorphism, while one population showed eight 18S and four 5S signals, respectively. Southern blot data confirms that 5S rDNA clusters present on two different chromosome pairs in Kosi river population contain non-transcribed spacers (NTS) of same length. In the present study, simultaneous localization of 45S and 5S rDNA by in situ hybridization helped us to develop the discrete population-specific markers in different geographically isolated populations of T. putitora. |
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