Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers
Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and inte...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
AMOSOVA, ALEXANDRA V. [verfasserIn] ZEMTSOVA, LYUDMILA V. [verfasserIn] GRUSHETSKAYA, ZOYA E. [verfasserIn] SAMATADZE, TATIANA E. [verfasserIn] MOZGOVA, GALINA V. [verfasserIn] PILYUK, YADVIGA E. [verfasserIn] VOLOVIK, VALENTINA T. [verfasserIn] MELNIKOVA, NATALIA V. [verfasserIn] ZELENIN, ALEXANDR V. [verfasserIn] LEMESH, VALENTINA A. [verfasserIn] MURAVENKO, OLGA V. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of Genetics - Springer India, 1955, 93(2014), 1 vom: Apr., Seite 133-143 |
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| Übergeordnetes Werk: |
volume:93 ; year:2014 ; number:1 ; month:04 ; pages:133-143 |
| Links: |
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| DOI / URN: |
10.1007/s12041-014-0351-6 |
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| Katalog-ID: |
SPR024284882 |
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| 245 | 1 | 0 | |a Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers |
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| 520 | |a Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. | ||
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10.1007/s12041-014-0351-6 doi (DE-627)SPR024284882 (SPR)s12041-014-0351-6-e DE-627 ger DE-627 rakwb eng AMOSOVA, ALEXANDRA V. verfasserin aut Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. C-banding (dpeaa)DE-He213 fluorescence (dpeaa)DE-He213 hybridization (dpeaa)DE-He213 gene (dpeaa)DE-He213 idiogram (dpeaa)DE-He213 rDNA sites (dpeaa)DE-He213 ZEMTSOVA, LYUDMILA V. verfasserin aut GRUSHETSKAYA, ZOYA E. verfasserin aut SAMATADZE, TATIANA E. verfasserin aut MOZGOVA, GALINA V. verfasserin aut PILYUK, YADVIGA E. verfasserin aut VOLOVIK, VALENTINA T. verfasserin aut MELNIKOVA, NATALIA V. verfasserin aut ZELENIN, ALEXANDR V. verfasserin aut LEMESH, VALENTINA A. verfasserin aut MURAVENKO, OLGA V. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 93(2014), 1 vom: Apr., Seite 133-143 (DE-627)SPR024069582 nnns volume:93 year:2014 number:1 month:04 pages:133-143 https://dx.doi.org/10.1007/s12041-014-0351-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 93 2014 1 04 133-143 |
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10.1007/s12041-014-0351-6 doi (DE-627)SPR024284882 (SPR)s12041-014-0351-6-e DE-627 ger DE-627 rakwb eng AMOSOVA, ALEXANDRA V. verfasserin aut Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. C-banding (dpeaa)DE-He213 fluorescence (dpeaa)DE-He213 hybridization (dpeaa)DE-He213 gene (dpeaa)DE-He213 idiogram (dpeaa)DE-He213 rDNA sites (dpeaa)DE-He213 ZEMTSOVA, LYUDMILA V. verfasserin aut GRUSHETSKAYA, ZOYA E. verfasserin aut SAMATADZE, TATIANA E. verfasserin aut MOZGOVA, GALINA V. verfasserin aut PILYUK, YADVIGA E. verfasserin aut VOLOVIK, VALENTINA T. verfasserin aut MELNIKOVA, NATALIA V. verfasserin aut ZELENIN, ALEXANDR V. verfasserin aut LEMESH, VALENTINA A. verfasserin aut MURAVENKO, OLGA V. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 93(2014), 1 vom: Apr., Seite 133-143 (DE-627)SPR024069582 nnns volume:93 year:2014 number:1 month:04 pages:133-143 https://dx.doi.org/10.1007/s12041-014-0351-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 93 2014 1 04 133-143 |
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10.1007/s12041-014-0351-6 doi (DE-627)SPR024284882 (SPR)s12041-014-0351-6-e DE-627 ger DE-627 rakwb eng AMOSOVA, ALEXANDRA V. verfasserin aut Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. C-banding (dpeaa)DE-He213 fluorescence (dpeaa)DE-He213 hybridization (dpeaa)DE-He213 gene (dpeaa)DE-He213 idiogram (dpeaa)DE-He213 rDNA sites (dpeaa)DE-He213 ZEMTSOVA, LYUDMILA V. verfasserin aut GRUSHETSKAYA, ZOYA E. verfasserin aut SAMATADZE, TATIANA E. verfasserin aut MOZGOVA, GALINA V. verfasserin aut PILYUK, YADVIGA E. verfasserin aut VOLOVIK, VALENTINA T. verfasserin aut MELNIKOVA, NATALIA V. verfasserin aut ZELENIN, ALEXANDR V. verfasserin aut LEMESH, VALENTINA A. verfasserin aut MURAVENKO, OLGA V. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 93(2014), 1 vom: Apr., Seite 133-143 (DE-627)SPR024069582 nnns volume:93 year:2014 number:1 month:04 pages:133-143 https://dx.doi.org/10.1007/s12041-014-0351-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 93 2014 1 04 133-143 |
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10.1007/s12041-014-0351-6 doi (DE-627)SPR024284882 (SPR)s12041-014-0351-6-e DE-627 ger DE-627 rakwb eng AMOSOVA, ALEXANDRA V. verfasserin aut Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. C-banding (dpeaa)DE-He213 fluorescence (dpeaa)DE-He213 hybridization (dpeaa)DE-He213 gene (dpeaa)DE-He213 idiogram (dpeaa)DE-He213 rDNA sites (dpeaa)DE-He213 ZEMTSOVA, LYUDMILA V. verfasserin aut GRUSHETSKAYA, ZOYA E. verfasserin aut SAMATADZE, TATIANA E. verfasserin aut MOZGOVA, GALINA V. verfasserin aut PILYUK, YADVIGA E. verfasserin aut VOLOVIK, VALENTINA T. verfasserin aut MELNIKOVA, NATALIA V. verfasserin aut ZELENIN, ALEXANDR V. verfasserin aut LEMESH, VALENTINA A. verfasserin aut MURAVENKO, OLGA V. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 93(2014), 1 vom: Apr., Seite 133-143 (DE-627)SPR024069582 nnns volume:93 year:2014 number:1 month:04 pages:133-143 https://dx.doi.org/10.1007/s12041-014-0351-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 93 2014 1 04 133-143 |
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10.1007/s12041-014-0351-6 doi (DE-627)SPR024284882 (SPR)s12041-014-0351-6-e DE-627 ger DE-627 rakwb eng AMOSOVA, ALEXANDRA V. verfasserin aut Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. C-banding (dpeaa)DE-He213 fluorescence (dpeaa)DE-He213 hybridization (dpeaa)DE-He213 gene (dpeaa)DE-He213 idiogram (dpeaa)DE-He213 rDNA sites (dpeaa)DE-He213 ZEMTSOVA, LYUDMILA V. verfasserin aut GRUSHETSKAYA, ZOYA E. verfasserin aut SAMATADZE, TATIANA E. verfasserin aut MOZGOVA, GALINA V. verfasserin aut PILYUK, YADVIGA E. verfasserin aut VOLOVIK, VALENTINA T. verfasserin aut MELNIKOVA, NATALIA V. verfasserin aut ZELENIN, ALEXANDR V. verfasserin aut LEMESH, VALENTINA A. verfasserin aut MURAVENKO, OLGA V. verfasserin aut Enthalten in Journal of Genetics Springer India, 1955 93(2014), 1 vom: Apr., Seite 133-143 (DE-627)SPR024069582 nnns volume:93 year:2014 number:1 month:04 pages:133-143 https://dx.doi.org/10.1007/s12041-014-0351-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2002 GBV_ILN_2004 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2012 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2088 GBV_ILN_2190 GBV_ILN_2244 AR 93 2014 1 04 133-143 |
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Enthalten in Journal of Genetics 93(2014), 1 vom: Apr., Seite 133-143 volume:93 year:2014 number:1 month:04 pages:133-143 |
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AMOSOVA, ALEXANDRA V. @@aut@@ ZEMTSOVA, LYUDMILA V. @@aut@@ GRUSHETSKAYA, ZOYA E. @@aut@@ SAMATADZE, TATIANA E. @@aut@@ MOZGOVA, GALINA V. @@aut@@ PILYUK, YADVIGA E. @@aut@@ VOLOVIK, VALENTINA T. @@aut@@ MELNIKOVA, NATALIA V. @@aut@@ ZELENIN, ALEXANDR V. @@aut@@ LEMESH, VALENTINA A. @@aut@@ MURAVENKO, OLGA V. @@aut@@ |
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AMOSOVA, ALEXANDRA V. |
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AMOSOVA, ALEXANDRA V. misc C-banding misc fluorescence misc hybridization misc gene misc idiogram misc rDNA sites Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers |
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Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers C-banding (dpeaa)DE-He213 fluorescence (dpeaa)DE-He213 hybridization (dpeaa)DE-He213 gene (dpeaa)DE-He213 idiogram (dpeaa)DE-He213 rDNA sites (dpeaa)DE-He213 |
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Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers |
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Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers |
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AMOSOVA, ALEXANDRA V. |
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AMOSOVA, ALEXANDRA V. ZEMTSOVA, LYUDMILA V. GRUSHETSKAYA, ZOYA E. SAMATADZE, TATIANA E. MOZGOVA, GALINA V. PILYUK, YADVIGA E. VOLOVIK, VALENTINA T. MELNIKOVA, NATALIA V. ZELENIN, ALEXANDR V. LEMESH, VALENTINA A. MURAVENKO, OLGA V. |
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intraspecific chromosomal and genetic polymorphism in brassica napus l. detected by cytogenetic and molecular markers |
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Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers |
| abstract |
Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. |
| abstractGer |
Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. |
| abstract_unstemmed |
Abstract The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape. |
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Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers |
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ZEMTSOVA, LYUDMILA V. GRUSHETSKAYA, ZOYA E. SAMATADZE, TATIANA E. MOZGOVA, GALINA V. PILYUK, YADVIGA E. VOLOVIK, VALENTINA T. MELNIKOVA, NATALIA V. ZELENIN, ALEXANDR V. LEMESH, VALENTINA A. MURAVENKO, OLGA V. |
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ZEMTSOVA, LYUDMILA V. GRUSHETSKAYA, ZOYA E. SAMATADZE, TATIANA E. MOZGOVA, GALINA V. PILYUK, YADVIGA E. VOLOVIK, VALENTINA T. MELNIKOVA, NATALIA V. ZELENIN, ALEXANDR V. LEMESH, VALENTINA A. MURAVENKO, OLGA V. |
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