APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase
Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang I...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Park, Won Sun [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Anmerkung: |
© The Pharmaceutical Society of Korea 2008 |
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| Übergeordnetes Werk: |
Enthalten in: Archives of pharmacal research - Berlin : Springer, 1978, 31(2008), 10 vom: Okt., Seite 1291-1301 |
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| Übergeordnetes Werk: |
volume:31 ; year:2008 ; number:10 ; month:10 ; pages:1291-1301 |
| Links: |
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| DOI / URN: |
10.1007/s12272-001-2109-y |
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| Katalog-ID: |
SPR024670480 |
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| 100 | 1 | |a Park, Won Sun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase |
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| 520 | |a Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. | ||
| 700 | 1 | |a Ko, Eun A. |4 aut | |
| 700 | 1 | |a Jung, In Duk |4 aut | |
| 700 | 1 | |a Son, Youn Kyoung |4 aut | |
| 700 | 1 | |a Kim, Hyoung Kyu |4 aut | |
| 700 | 1 | |a Kim, Nari |4 aut | |
| 700 | 1 | |a Park, So Youn |4 aut | |
| 700 | 1 | |a Hong, Ki Whan |4 aut | |
| 700 | 1 | |a Park, Yeong-Min |4 aut | |
| 700 | 1 | |a Choi, Tae-Hoon |4 aut | |
| 700 | 1 | |a Han, Jin |4 aut | |
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| 912 | |a GBV_ILN_2061 | ||
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10.1007/s12272-001-2109-y doi (DE-627)SPR024670480 (SPR)s12272-001-2109-y-e DE-627 ger DE-627 rakwb eng Park, Won Sun verfasserin aut APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Pharmaceutical Society of Korea 2008 Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. Ko, Eun A. aut Jung, In Duk aut Son, Youn Kyoung aut Kim, Hyoung Kyu aut Kim, Nari aut Park, So Youn aut Hong, Ki Whan aut Park, Yeong-Min aut Choi, Tae-Hoon aut Han, Jin aut Enthalten in Archives of pharmacal research Berlin : Springer, 1978 31(2008), 10 vom: Okt., Seite 1291-1301 (DE-627)363761802 (DE-600)2106388-6 1976-3786 nnns volume:31 year:2008 number:10 month:10 pages:1291-1301 https://dx.doi.org/10.1007/s12272-001-2109-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 31 2008 10 10 1291-1301 |
| spelling |
10.1007/s12272-001-2109-y doi (DE-627)SPR024670480 (SPR)s12272-001-2109-y-e DE-627 ger DE-627 rakwb eng Park, Won Sun verfasserin aut APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Pharmaceutical Society of Korea 2008 Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. Ko, Eun A. aut Jung, In Duk aut Son, Youn Kyoung aut Kim, Hyoung Kyu aut Kim, Nari aut Park, So Youn aut Hong, Ki Whan aut Park, Yeong-Min aut Choi, Tae-Hoon aut Han, Jin aut Enthalten in Archives of pharmacal research Berlin : Springer, 1978 31(2008), 10 vom: Okt., Seite 1291-1301 (DE-627)363761802 (DE-600)2106388-6 1976-3786 nnns volume:31 year:2008 number:10 month:10 pages:1291-1301 https://dx.doi.org/10.1007/s12272-001-2109-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 31 2008 10 10 1291-1301 |
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10.1007/s12272-001-2109-y doi (DE-627)SPR024670480 (SPR)s12272-001-2109-y-e DE-627 ger DE-627 rakwb eng Park, Won Sun verfasserin aut APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Pharmaceutical Society of Korea 2008 Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. Ko, Eun A. aut Jung, In Duk aut Son, Youn Kyoung aut Kim, Hyoung Kyu aut Kim, Nari aut Park, So Youn aut Hong, Ki Whan aut Park, Yeong-Min aut Choi, Tae-Hoon aut Han, Jin aut Enthalten in Archives of pharmacal research Berlin : Springer, 1978 31(2008), 10 vom: Okt., Seite 1291-1301 (DE-627)363761802 (DE-600)2106388-6 1976-3786 nnns volume:31 year:2008 number:10 month:10 pages:1291-1301 https://dx.doi.org/10.1007/s12272-001-2109-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 31 2008 10 10 1291-1301 |
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10.1007/s12272-001-2109-y doi (DE-627)SPR024670480 (SPR)s12272-001-2109-y-e DE-627 ger DE-627 rakwb eng Park, Won Sun verfasserin aut APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Pharmaceutical Society of Korea 2008 Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. Ko, Eun A. aut Jung, In Duk aut Son, Youn Kyoung aut Kim, Hyoung Kyu aut Kim, Nari aut Park, So Youn aut Hong, Ki Whan aut Park, Yeong-Min aut Choi, Tae-Hoon aut Han, Jin aut Enthalten in Archives of pharmacal research Berlin : Springer, 1978 31(2008), 10 vom: Okt., Seite 1291-1301 (DE-627)363761802 (DE-600)2106388-6 1976-3786 nnns volume:31 year:2008 number:10 month:10 pages:1291-1301 https://dx.doi.org/10.1007/s12272-001-2109-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 31 2008 10 10 1291-1301 |
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10.1007/s12272-001-2109-y doi (DE-627)SPR024670480 (SPR)s12272-001-2109-y-e DE-627 ger DE-627 rakwb eng Park, Won Sun verfasserin aut APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Pharmaceutical Society of Korea 2008 Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. Ko, Eun A. aut Jung, In Duk aut Son, Youn Kyoung aut Kim, Hyoung Kyu aut Kim, Nari aut Park, So Youn aut Hong, Ki Whan aut Park, Yeong-Min aut Choi, Tae-Hoon aut Han, Jin aut Enthalten in Archives of pharmacal research Berlin : Springer, 1978 31(2008), 10 vom: Okt., Seite 1291-1301 (DE-627)363761802 (DE-600)2106388-6 1976-3786 nnns volume:31 year:2008 number:10 month:10 pages:1291-1301 https://dx.doi.org/10.1007/s12272-001-2109-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 31 2008 10 10 1291-1301 |
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Park, Won Sun @@aut@@ Ko, Eun A. @@aut@@ Jung, In Duk @@aut@@ Son, Youn Kyoung @@aut@@ Kim, Hyoung Kyu @@aut@@ Kim, Nari @@aut@@ Park, So Youn @@aut@@ Hong, Ki Whan @@aut@@ Park, Yeong-Min @@aut@@ Choi, Tae-Hoon @@aut@@ Han, Jin @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR024670480</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519145003.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2008 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s12272-001-2109-y</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR024670480</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12272-001-2109-y-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Park, Won Sun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Pharmaceutical Society of Korea 2008</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. 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Park, Won Sun |
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Park, Won Sun APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase |
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APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase |
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APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase |
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(DE-627)SPR024670480 (SPR)s12272-001-2109-y-e |
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APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase |
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Park, Won Sun |
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Park, Won Sun Ko, Eun A. Jung, In Duk Son, Youn Kyoung Kim, Hyoung Kyu Kim, Nari Park, So Youn Hong, Ki Whan Park, Yeong-Min Choi, Tae-Hoon Han, Jin |
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Park, Won Sun |
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10.1007/s12272-001-2109-y |
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ape1/ref-1 promotes the effect of angiotensin ii on $ ca^{2+} $-activated $ k^{+} $ channel in human endothelial cells via suppression of nadph oxidase |
| title_auth |
APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase |
| abstract |
Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. © The Pharmaceutical Society of Korea 2008 |
| abstractGer |
Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. © The Pharmaceutical Society of Korea 2008 |
| abstract_unstemmed |
Abstract The effects of angiotensin II (Ang II) on whole-cell large conductance $ Ca^{2+} $-activated $ K^{+} $ ($ BK_{Ca} $) currents was investigated in control and Apurinic/apyrimidinic endonuclease1/redox factor 1 (APE1/Ref-1)-overexpressing human umbilical vein endothelial cells (HUVECs). Ang II blocked the $ BK_{Ca} $ current in a dose-dependent fashion, and this inhibition was greater in APE1/Ref-1-overexpressing HUVECs than in control HUVECs (half-inhibition values of 102.81 ± 9.54 nM and 11.34 ± 0.39 nM in control and APE1/Ref-1-overexpressing HUVECs, respectively). Pretreatment with the NADPH oxidase inhibitor diphenyleneiodonium (DPI) or knock down of NADPH oxidase (p22 phox) using siRNA increased the inhibitory effect of Ang II on the $ BK_{Ca} $ currents, similar to the effect of APE1/Ref-1 overexpression. In addition, application of Ang II increased the superoxide and hydrogen peroxide levels in the control HUVECs but not in APE1/Ref-1-overexpressing HUVECs. Furthermore, direct application of hydrogen peroxide increased $ BK_{Ca} $ channel activity. Finally, the inhibitory effect of Ang II on the $ BK_{Ca} $ current was blocked by an antagonist of the Ang II type 1 ($ AT_{1} $) receptor in both control and APE1/Ref-1-overexpressing HUVECs. From these results, we conclude that the inhibitory effect of Ang II on $ BK_{Ca} $ channel function is NADPH oxidase-dependent and may be promoted by APE1/Ref-1. © The Pharmaceutical Society of Korea 2008 |
| collection_details |
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| container_issue |
10 |
| title_short |
APE1/Ref-1 promotes the effect of Angiotensin II on $ Ca^{2+} $-activated $ K^{+} $ channel in human endothelial cells via suppression of NADPH Oxidase |
| url |
https://dx.doi.org/10.1007/s12272-001-2109-y |
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| author2 |
Ko, Eun A. Jung, In Duk Son, Youn Kyoung Kim, Hyoung Kyu Kim, Nari Park, So Youn Hong, Ki Whan Park, Yeong-Min Choi, Tae-Hoon Han, Jin |
| author2Str |
Ko, Eun A. Jung, In Duk Son, Youn Kyoung Kim, Hyoung Kyu Kim, Nari Park, So Youn Hong, Ki Whan Park, Yeong-Min Choi, Tae-Hoon Han, Jin |
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| doi_str |
10.1007/s12272-001-2109-y |
| up_date |
2024-07-04T01:53:27.657Z |
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| score |
7.398529 |