Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures
Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide conta...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Valente, Gianpiero [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Anmerkung: |
© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Nano research - [S.l.] : Tsinghua Press, 2008, 9(2015), 3 vom: 28. Dez., Seite 644-662 |
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| Übergeordnetes Werk: |
volume:9 ; year:2015 ; number:3 ; day:28 ; month:12 ; pages:644-662 |
| Links: |
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| DOI / URN: |
10.1007/s12274-015-0944-2 |
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| Katalog-ID: |
SPR024717932 |
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| 100 | 1 | |a Valente, Gianpiero |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures |
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| 520 | |a Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. | ||
| 650 | 4 | |a nanocrystalline heterostructures |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a photoactive semiconductor |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a magnetic nanostructure |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a cyclic RGD peptide |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a αvβ3 integrin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a active targeting |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Depalo, Nicoletta |4 aut | |
| 700 | 1 | |a de Paola, Ivan |4 aut | |
| 700 | 1 | |a Iacobazzi, Rosa Maria |4 aut | |
| 700 | 1 | |a Denora, Nunzio |4 aut | |
| 700 | 1 | |a Laquintana, Valentino |4 aut | |
| 700 | 1 | |a Comparelli, Roberto |4 aut | |
| 700 | 1 | |a Altamura, Emiliano |4 aut | |
| 700 | 1 | |a Latronico, Tiziana |4 aut | |
| 700 | 1 | |a Altomare, Michele |4 aut | |
| 700 | 1 | |a Fanizza, Elisabetta |4 aut | |
| 700 | 1 | |a Striccoli, Marinella |4 aut | |
| 700 | 1 | |a Agostiano, Angela |4 aut | |
| 700 | 1 | |a Saviano, Michele |4 aut | |
| 700 | 1 | |a Del Gatto, Annarita |4 aut | |
| 700 | 1 | |a Zaccaro, Laura |4 aut | |
| 700 | 1 | |a Curri, Maria Lucia |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nano research |d [S.l.] : Tsinghua Press, 2008 |g 9(2015), 3 vom: 28. Dez., Seite 644-662 |w (DE-627)57375361X |w (DE-600)2442216-2 |x 1998-0000 |7 nnns |
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| 912 | |a GBV_ILN_151 | ||
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| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_171 | ||
| 912 | |a GBV_ILN_187 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_250 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
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| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
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10.1007/s12274-015-0944-2 doi (DE-627)SPR024717932 (SPR)s12274-015-0944-2-e DE-627 ger DE-627 rakwb eng Valente, Gianpiero verfasserin aut Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. nanocrystalline heterostructures (dpeaa)DE-He213 photoactive semiconductor (dpeaa)DE-He213 magnetic nanostructure (dpeaa)DE-He213 cyclic RGD peptide (dpeaa)DE-He213 αvβ3 integrin (dpeaa)DE-He213 active targeting (dpeaa)DE-He213 Depalo, Nicoletta aut de Paola, Ivan aut Iacobazzi, Rosa Maria aut Denora, Nunzio aut Laquintana, Valentino aut Comparelli, Roberto aut Altamura, Emiliano aut Latronico, Tiziana aut Altomare, Michele aut Fanizza, Elisabetta aut Striccoli, Marinella aut Agostiano, Angela aut Saviano, Michele aut Del Gatto, Annarita aut Zaccaro, Laura aut Curri, Maria Lucia aut Enthalten in Nano research [S.l.] : Tsinghua Press, 2008 9(2015), 3 vom: 28. Dez., Seite 644-662 (DE-627)57375361X (DE-600)2442216-2 1998-0000 nnns volume:9 year:2015 number:3 day:28 month:12 pages:644-662 https://dx.doi.org/10.1007/s12274-015-0944-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 3 28 12 644-662 |
| spelling |
10.1007/s12274-015-0944-2 doi (DE-627)SPR024717932 (SPR)s12274-015-0944-2-e DE-627 ger DE-627 rakwb eng Valente, Gianpiero verfasserin aut Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. nanocrystalline heterostructures (dpeaa)DE-He213 photoactive semiconductor (dpeaa)DE-He213 magnetic nanostructure (dpeaa)DE-He213 cyclic RGD peptide (dpeaa)DE-He213 αvβ3 integrin (dpeaa)DE-He213 active targeting (dpeaa)DE-He213 Depalo, Nicoletta aut de Paola, Ivan aut Iacobazzi, Rosa Maria aut Denora, Nunzio aut Laquintana, Valentino aut Comparelli, Roberto aut Altamura, Emiliano aut Latronico, Tiziana aut Altomare, Michele aut Fanizza, Elisabetta aut Striccoli, Marinella aut Agostiano, Angela aut Saviano, Michele aut Del Gatto, Annarita aut Zaccaro, Laura aut Curri, Maria Lucia aut Enthalten in Nano research [S.l.] : Tsinghua Press, 2008 9(2015), 3 vom: 28. Dez., Seite 644-662 (DE-627)57375361X (DE-600)2442216-2 1998-0000 nnns volume:9 year:2015 number:3 day:28 month:12 pages:644-662 https://dx.doi.org/10.1007/s12274-015-0944-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 3 28 12 644-662 |
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10.1007/s12274-015-0944-2 doi (DE-627)SPR024717932 (SPR)s12274-015-0944-2-e DE-627 ger DE-627 rakwb eng Valente, Gianpiero verfasserin aut Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. nanocrystalline heterostructures (dpeaa)DE-He213 photoactive semiconductor (dpeaa)DE-He213 magnetic nanostructure (dpeaa)DE-He213 cyclic RGD peptide (dpeaa)DE-He213 αvβ3 integrin (dpeaa)DE-He213 active targeting (dpeaa)DE-He213 Depalo, Nicoletta aut de Paola, Ivan aut Iacobazzi, Rosa Maria aut Denora, Nunzio aut Laquintana, Valentino aut Comparelli, Roberto aut Altamura, Emiliano aut Latronico, Tiziana aut Altomare, Michele aut Fanizza, Elisabetta aut Striccoli, Marinella aut Agostiano, Angela aut Saviano, Michele aut Del Gatto, Annarita aut Zaccaro, Laura aut Curri, Maria Lucia aut Enthalten in Nano research [S.l.] : Tsinghua Press, 2008 9(2015), 3 vom: 28. Dez., Seite 644-662 (DE-627)57375361X (DE-600)2442216-2 1998-0000 nnns volume:9 year:2015 number:3 day:28 month:12 pages:644-662 https://dx.doi.org/10.1007/s12274-015-0944-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 3 28 12 644-662 |
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10.1007/s12274-015-0944-2 doi (DE-627)SPR024717932 (SPR)s12274-015-0944-2-e DE-627 ger DE-627 rakwb eng Valente, Gianpiero verfasserin aut Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. nanocrystalline heterostructures (dpeaa)DE-He213 photoactive semiconductor (dpeaa)DE-He213 magnetic nanostructure (dpeaa)DE-He213 cyclic RGD peptide (dpeaa)DE-He213 αvβ3 integrin (dpeaa)DE-He213 active targeting (dpeaa)DE-He213 Depalo, Nicoletta aut de Paola, Ivan aut Iacobazzi, Rosa Maria aut Denora, Nunzio aut Laquintana, Valentino aut Comparelli, Roberto aut Altamura, Emiliano aut Latronico, Tiziana aut Altomare, Michele aut Fanizza, Elisabetta aut Striccoli, Marinella aut Agostiano, Angela aut Saviano, Michele aut Del Gatto, Annarita aut Zaccaro, Laura aut Curri, Maria Lucia aut Enthalten in Nano research [S.l.] : Tsinghua Press, 2008 9(2015), 3 vom: 28. Dez., Seite 644-662 (DE-627)57375361X (DE-600)2442216-2 1998-0000 nnns volume:9 year:2015 number:3 day:28 month:12 pages:644-662 https://dx.doi.org/10.1007/s12274-015-0944-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 3 28 12 644-662 |
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10.1007/s12274-015-0944-2 doi (DE-627)SPR024717932 (SPR)s12274-015-0944-2-e DE-627 ger DE-627 rakwb eng Valente, Gianpiero verfasserin aut Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. nanocrystalline heterostructures (dpeaa)DE-He213 photoactive semiconductor (dpeaa)DE-He213 magnetic nanostructure (dpeaa)DE-He213 cyclic RGD peptide (dpeaa)DE-He213 αvβ3 integrin (dpeaa)DE-He213 active targeting (dpeaa)DE-He213 Depalo, Nicoletta aut de Paola, Ivan aut Iacobazzi, Rosa Maria aut Denora, Nunzio aut Laquintana, Valentino aut Comparelli, Roberto aut Altamura, Emiliano aut Latronico, Tiziana aut Altomare, Michele aut Fanizza, Elisabetta aut Striccoli, Marinella aut Agostiano, Angela aut Saviano, Michele aut Del Gatto, Annarita aut Zaccaro, Laura aut Curri, Maria Lucia aut Enthalten in Nano research [S.l.] : Tsinghua Press, 2008 9(2015), 3 vom: 28. Dez., Seite 644-662 (DE-627)57375361X (DE-600)2442216-2 1998-0000 nnns volume:9 year:2015 number:3 day:28 month:12 pages:644-662 https://dx.doi.org/10.1007/s12274-015-0944-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 9 2015 3 28 12 644-662 |
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Enthalten in Nano research 9(2015), 3 vom: 28. Dez., Seite 644-662 volume:9 year:2015 number:3 day:28 month:12 pages:644-662 |
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Enthalten in Nano research 9(2015), 3 vom: 28. Dez., Seite 644-662 volume:9 year:2015 number:3 day:28 month:12 pages:644-662 |
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nanocrystalline heterostructures photoactive semiconductor magnetic nanostructure cyclic RGD peptide αvβ3 integrin active targeting |
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Valente, Gianpiero @@aut@@ Depalo, Nicoletta @@aut@@ de Paola, Ivan @@aut@@ Iacobazzi, Rosa Maria @@aut@@ Denora, Nunzio @@aut@@ Laquintana, Valentino @@aut@@ Comparelli, Roberto @@aut@@ Altamura, Emiliano @@aut@@ Latronico, Tiziana @@aut@@ Altomare, Michele @@aut@@ Fanizza, Elisabetta @@aut@@ Striccoli, Marinella @@aut@@ Agostiano, Angela @@aut@@ Saviano, Michele @@aut@@ Del Gatto, Annarita @@aut@@ Zaccaro, Laura @@aut@@ Curri, Maria Lucia @@aut@@ |
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2015-12-28T00:00:00Z |
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BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. 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Valente, Gianpiero |
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Valente, Gianpiero misc nanocrystalline heterostructures misc photoactive semiconductor misc magnetic nanostructure misc cyclic RGD peptide misc αvβ3 integrin misc active targeting Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures |
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Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures nanocrystalline heterostructures (dpeaa)DE-He213 photoactive semiconductor (dpeaa)DE-He213 magnetic nanostructure (dpeaa)DE-He213 cyclic RGD peptide (dpeaa)DE-He213 αvβ3 integrin (dpeaa)DE-He213 active targeting (dpeaa)DE-He213 |
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misc nanocrystalline heterostructures misc photoactive semiconductor misc magnetic nanostructure misc cyclic RGD peptide misc αvβ3 integrin misc active targeting |
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misc nanocrystalline heterostructures misc photoactive semiconductor misc magnetic nanostructure misc cyclic RGD peptide misc αvβ3 integrin misc active targeting |
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Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures |
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Valente, Gianpiero Depalo, Nicoletta de Paola, Ivan Iacobazzi, Rosa Maria Denora, Nunzio Laquintana, Valentino Comparelli, Roberto Altamura, Emiliano Latronico, Tiziana Altomare, Michele Fanizza, Elisabetta Striccoli, Marinella Agostiano, Angela Saviano, Michele Del Gatto, Annarita Zaccaro, Laura Curri, Maria Lucia |
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Elektronische Aufsätze |
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Valente, Gianpiero |
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10.1007/s12274-015-0944-2 |
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integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures |
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Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures |
| abstract |
Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 |
| abstractGer |
Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 |
| abstract_unstemmed |
Abstract Binary asymmetric nanocrystals (BNCs), composed of a photoactive $ TiO_{2} $ nanorod joined with a superparamagnetic γ-$ Fe_{2} %$ O_{3} $ spherical domain, were embedded in polyethylene glycol modified phospholipid micelle and successfully bioconjugated to a suitably designed peptide containing an RGD motif. BNCs represent a relevant multifunctional nanomaterial, owing to the coexistence of two distinct domains in one particle, characterized by high photoactivity and magnetic properties, that is particularly suited for use as a phototherapy and hyperthermia agent as well as a magnetic probe in biological imaging. We selected the RGD motif in order to target integrin expressed on activated endothelial cells and several types of cancer cells. The prepared RGD-peptide/BNC conjugates, comprehensively monitored by using complementary optical and structural techniques, demonstrated a high stability and uniform dispersibility in biological media. The cytotoxicity of the RGD-peptide/BNC conjugates was studied in vitro. The cellular uptake of RGD-peptide conjugates in the cells, assessed by means of two distinct approaches, namely confocal microscopy analysis and emission spectroscopy determination in cell lysates, displayed selectivity of the RGD-peptide-BNC conjugate for the αvβ3 integrin. These RGD-peptide-BNC conjugates have a high potential for theranostic treatment of cancer. © Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016 |
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Integrin-targeting with peptide-bioconjugated semiconductor-magnetic nanocrystalline heterostructures |
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Depalo, Nicoletta de Paola, Ivan Iacobazzi, Rosa Maria Denora, Nunzio Laquintana, Valentino Comparelli, Roberto Altamura, Emiliano Latronico, Tiziana Altomare, Michele Fanizza, Elisabetta Striccoli, Marinella Agostiano, Angela Saviano, Michele Del Gatto, Annarita Zaccaro, Laura Curri, Maria Lucia |
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| score |
7.4011717 |