Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers
Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department o...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chandrasene, G. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2003 |
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| Schlagwörter: |
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| Anmerkung: |
© Society for Sugar Research & Promotion 1999 |
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| Übergeordnetes Werk: |
Enthalten in: Sugar tech - Neu Delhi : Springer India, 1999, 5(2003), 4 vom: 01. Dez., Seite 237-241 |
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| Übergeordnetes Werk: |
volume:5 ; year:2003 ; number:4 ; day:01 ; month:12 ; pages:237-241 |
| Links: |
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| DOI / URN: |
10.1007/BF02942479 |
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| Katalog-ID: |
SPR025001914 |
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| 100 | 1 | |a Chandrasene, G. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers |
| 264 | 1 | |c 2003 | |
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| 520 | |a Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. | ||
| 650 | 4 | |a DNA extraction |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a FTA™ paper |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a grassy shoot disease |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a phytoplasma |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a sugarcane |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a white leaf disease |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Brune, A. E. |4 aut | |
| 700 | 1 | |a Rutherford, R. S. |4 aut | |
| 700 | 1 | |a Dharmawardena, N. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Sugar tech |d Neu Delhi : Springer India, 1999 |g 5(2003), 4 vom: 01. Dez., Seite 237-241 |w (DE-627)570507685 |w (DE-600)2433394-3 |x 0974-0740 |7 nnns |
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| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/BF02942479 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a GBV_ILN_11 | ||
| 912 | |a GBV_ILN_20 | ||
| 912 | |a GBV_ILN_22 | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_65 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_100 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_171 | ||
| 912 | |a GBV_ILN_187 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_250 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 912 | |a GBV_ILN_4700 | ||
| 951 | |a AR | ||
| 952 | |d 5 |j 2003 |e 4 |b 01 |c 12 |h 237-241 | ||
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2003 |
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2003 |
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10.1007/BF02942479 doi (DE-627)SPR025001914 (SPR)BF02942479-e DE-627 ger DE-627 rakwb eng Chandrasene, G. verfasserin aut Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 1999 Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. DNA extraction (dpeaa)DE-He213 FTA™ paper (dpeaa)DE-He213 grassy shoot disease (dpeaa)DE-He213 phytoplasma (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 white leaf disease (dpeaa)DE-He213 Brune, A. E. aut Rutherford, R. S. aut Dharmawardena, N. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 5(2003), 4 vom: 01. Dez., Seite 237-241 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:5 year:2003 number:4 day:01 month:12 pages:237-241 https://dx.doi.org/10.1007/BF02942479 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2003 4 01 12 237-241 |
| spelling |
10.1007/BF02942479 doi (DE-627)SPR025001914 (SPR)BF02942479-e DE-627 ger DE-627 rakwb eng Chandrasene, G. verfasserin aut Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 1999 Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. DNA extraction (dpeaa)DE-He213 FTA™ paper (dpeaa)DE-He213 grassy shoot disease (dpeaa)DE-He213 phytoplasma (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 white leaf disease (dpeaa)DE-He213 Brune, A. E. aut Rutherford, R. S. aut Dharmawardena, N. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 5(2003), 4 vom: 01. Dez., Seite 237-241 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:5 year:2003 number:4 day:01 month:12 pages:237-241 https://dx.doi.org/10.1007/BF02942479 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2003 4 01 12 237-241 |
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10.1007/BF02942479 doi (DE-627)SPR025001914 (SPR)BF02942479-e DE-627 ger DE-627 rakwb eng Chandrasene, G. verfasserin aut Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 1999 Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. DNA extraction (dpeaa)DE-He213 FTA™ paper (dpeaa)DE-He213 grassy shoot disease (dpeaa)DE-He213 phytoplasma (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 white leaf disease (dpeaa)DE-He213 Brune, A. E. aut Rutherford, R. S. aut Dharmawardena, N. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 5(2003), 4 vom: 01. Dez., Seite 237-241 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:5 year:2003 number:4 day:01 month:12 pages:237-241 https://dx.doi.org/10.1007/BF02942479 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2003 4 01 12 237-241 |
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10.1007/BF02942479 doi (DE-627)SPR025001914 (SPR)BF02942479-e DE-627 ger DE-627 rakwb eng Chandrasene, G. verfasserin aut Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 1999 Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. DNA extraction (dpeaa)DE-He213 FTA™ paper (dpeaa)DE-He213 grassy shoot disease (dpeaa)DE-He213 phytoplasma (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 white leaf disease (dpeaa)DE-He213 Brune, A. E. aut Rutherford, R. S. aut Dharmawardena, N. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 5(2003), 4 vom: 01. Dez., Seite 237-241 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:5 year:2003 number:4 day:01 month:12 pages:237-241 https://dx.doi.org/10.1007/BF02942479 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2003 4 01 12 237-241 |
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10.1007/BF02942479 doi (DE-627)SPR025001914 (SPR)BF02942479-e DE-627 ger DE-627 rakwb eng Chandrasene, G. verfasserin aut Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 1999 Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. DNA extraction (dpeaa)DE-He213 FTA™ paper (dpeaa)DE-He213 grassy shoot disease (dpeaa)DE-He213 phytoplasma (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 white leaf disease (dpeaa)DE-He213 Brune, A. E. aut Rutherford, R. S. aut Dharmawardena, N. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 5(2003), 4 vom: 01. Dez., Seite 237-241 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:5 year:2003 number:4 day:01 month:12 pages:237-241 https://dx.doi.org/10.1007/BF02942479 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 5 2003 4 01 12 237-241 |
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| author |
Chandrasene, G. |
| spellingShingle |
Chandrasene, G. misc DNA extraction misc FTA™ paper misc grassy shoot disease misc phytoplasma misc sugarcane misc white leaf disease Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers |
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Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers DNA extraction (dpeaa)DE-He213 FTA™ paper (dpeaa)DE-He213 grassy shoot disease (dpeaa)DE-He213 phytoplasma (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 white leaf disease (dpeaa)DE-He213 |
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misc DNA extraction misc FTA™ paper misc grassy shoot disease misc phytoplasma misc sugarcane misc white leaf disease |
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Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers |
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(DE-627)SPR025001914 (SPR)BF02942479-e |
| title_full |
Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers |
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Chandrasene, G. |
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Sugar tech |
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Chandrasene, G. Brune, A. E. Rutherford, R. S. Dharmawardena, N. |
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Chandrasene, G. |
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10.1007/BF02942479 |
| title_sort |
detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers |
| title_auth |
Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers |
| abstract |
Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. © Society for Sugar Research & Promotion 1999 |
| abstractGer |
Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. © Society for Sugar Research & Promotion 1999 |
| abstract_unstemmed |
Abstract The most reliable and rapid detection method has been the use of nested PCR directed to conserved sequences in the 16S rRNA gene. DNA isolation follows a phytoplasmal enrichment procedure. This process is both expensive and time-consuming. The present study was conducted at the Department of Pathology, South African Sugar Association Experiment Station, using sugarcane leaves and grass samples with GS, WL and Bermuda Grass White Leaf (BGWL) symptoms collected in Sri Lanka to investigate the use of FTA™ paper (Whatman BioScience) for DNA extraction and the use of a radioactive probe in conjunction with a tissue blot procedure to detect phytoplasmas in sugarcane leaf samples exhibiting symptoms of GS and SCWL. Bermuda grass leaf samples showing symptoms of Bermuda Grass White Leaf (BGWL) were also included in the study. It was found that the FTA™ paper method was a rapid and cost effective in detecting sugarcane phytoplasma compared to the enrichment method. © Society for Sugar Research & Promotion 1999 |
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Detection of phytoplasmas associated with grassy shoot and white leaf diseases of sugarcane in sri lanka using fta™ papers |
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Brune, A. E. Rutherford, R. S. Dharmawardena, N. |
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2024-07-04T03:07:59.125Z |
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