Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression
Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Watt, D. A. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© Society for Sugar Research & Promotion 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Sugar tech - Neu Delhi : Springer India, 1999, 12(2010), 2 vom: Juni, Seite 85-90 |
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| Übergeordnetes Werk: |
volume:12 ; year:2010 ; number:2 ; month:06 ; pages:85-90 |
| Links: |
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| DOI / URN: |
10.1007/s12355-010-0018-4 |
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| Katalog-ID: |
SPR025006215 |
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| 520 | |a Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. | ||
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| 700 | 1 | |a Sweby, D. L. |4 aut | |
| 700 | 1 | |a Potier, B. A. M. |4 aut | |
| 700 | 1 | |a Snyman, S. J. |4 aut | |
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10.1007/s12355-010-0018-4 doi (DE-627)SPR025006215 (SPR)s12355-010-0018-4-e DE-627 ger DE-627 rakwb eng Watt, D. A. verfasserin aut Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 2010 Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. micro-propagation (dpeaa)DE-He213 genetic transformation (dpeaa)DE-He213 gene promoters (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 Sweby, D. L. aut Potier, B. A. M. aut Snyman, S. J. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 12(2010), 2 vom: Juni, Seite 85-90 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:12 year:2010 number:2 month:06 pages:85-90 https://dx.doi.org/10.1007/s12355-010-0018-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2010 2 06 85-90 |
| spelling |
10.1007/s12355-010-0018-4 doi (DE-627)SPR025006215 (SPR)s12355-010-0018-4-e DE-627 ger DE-627 rakwb eng Watt, D. A. verfasserin aut Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 2010 Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. micro-propagation (dpeaa)DE-He213 genetic transformation (dpeaa)DE-He213 gene promoters (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 Sweby, D. L. aut Potier, B. A. M. aut Snyman, S. J. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 12(2010), 2 vom: Juni, Seite 85-90 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:12 year:2010 number:2 month:06 pages:85-90 https://dx.doi.org/10.1007/s12355-010-0018-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2010 2 06 85-90 |
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10.1007/s12355-010-0018-4 doi (DE-627)SPR025006215 (SPR)s12355-010-0018-4-e DE-627 ger DE-627 rakwb eng Watt, D. A. verfasserin aut Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 2010 Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. micro-propagation (dpeaa)DE-He213 genetic transformation (dpeaa)DE-He213 gene promoters (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 Sweby, D. L. aut Potier, B. A. M. aut Snyman, S. J. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 12(2010), 2 vom: Juni, Seite 85-90 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:12 year:2010 number:2 month:06 pages:85-90 https://dx.doi.org/10.1007/s12355-010-0018-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2010 2 06 85-90 |
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10.1007/s12355-010-0018-4 doi (DE-627)SPR025006215 (SPR)s12355-010-0018-4-e DE-627 ger DE-627 rakwb eng Watt, D. A. verfasserin aut Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 2010 Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. micro-propagation (dpeaa)DE-He213 genetic transformation (dpeaa)DE-He213 gene promoters (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 Sweby, D. L. aut Potier, B. A. M. aut Snyman, S. J. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 12(2010), 2 vom: Juni, Seite 85-90 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:12 year:2010 number:2 month:06 pages:85-90 https://dx.doi.org/10.1007/s12355-010-0018-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2010 2 06 85-90 |
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10.1007/s12355-010-0018-4 doi (DE-627)SPR025006215 (SPR)s12355-010-0018-4-e DE-627 ger DE-627 rakwb eng Watt, D. A. verfasserin aut Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Society for Sugar Research & Promotion 2010 Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. micro-propagation (dpeaa)DE-He213 genetic transformation (dpeaa)DE-He213 gene promoters (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 Sweby, D. L. aut Potier, B. A. M. aut Snyman, S. J. aut Enthalten in Sugar tech Neu Delhi : Springer India, 1999 12(2010), 2 vom: Juni, Seite 85-90 (DE-627)570507685 (DE-600)2433394-3 0974-0740 nnns volume:12 year:2010 number:2 month:06 pages:85-90 https://dx.doi.org/10.1007/s12355-010-0018-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2010 2 06 85-90 |
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Watt, D. A. misc micro-propagation misc genetic transformation misc gene promoters misc sugarcane Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression |
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Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression micro-propagation (dpeaa)DE-He213 genetic transformation (dpeaa)DE-He213 gene promoters (dpeaa)DE-He213 sugarcane (dpeaa)DE-He213 |
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sugarcane genetic engineering research in south africa: from gene discovery to transgene expression |
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Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression |
| abstract |
Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. © Society for Sugar Research & Promotion 2010 |
| abstractGer |
Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. © Society for Sugar Research & Promotion 2010 |
| abstract_unstemmed |
Abstract Over the past 15 years, recombinant DNA and in vitro culture technologies have been used in concert at the South African Sugarcane Research Institute (SASRI) to genetically engineer sugarcane. The purpose of such research has been two-fold, viz. to establish proof-of-principle regarding the delivery of novel input and resistance traits to sugarcane and to investigate the genetic basis of sucrose accumulation. Underpinning these goals has been the in-house development of essential genetic resources, including the isolation of appropriate transgenes and gene promoter elements, and the optimisation of transformation and tissue culture technologies. With regard to the latter, emphasis has been placed on the development of tissue culture strategies that minimise the potential for somaclonal variation, while maximising the efficiency of germplasm transformation, selection, regeneration and acclimation. The isolation of promoters to drive high-level and targeted transgene expression, as well as the identification of DNA sequences with the potential to deliver the desired phenotype, have been strongly driven by advances in gene discovery and expression technologies. Early approaches relied on the analysis of Expressed Sequence Tag libraries constructed from sugarcane tissues at different developmental stages or challenged with selected abiotic and biotic stress agents. Subsequent improvements in DNA subtractive hybridisation technologies (e.g. suppression subtractive hybridisation) and gene expression analysis (e.g. Affymetrix® Sugarcane Genome GeneChip) provided new and powerful means for gene and promoter discovery. Reviewed here are the advances in in vitro culture, gene discovery and transgene expression research, which have formed the foundation of the genetic engineering program at SASRI. Also discussed are the opportunities and challenges in these areas of research that are presented by the availability of ultra-high throughput DNA sequencing (e.g. the 454 Sequencing System) for transcriptome analysis and technologies for rapid and accurate profiling of the sugarcane metabolomes. © Society for Sugar Research & Promotion 2010 |
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Sugarcane genetic engineering research in South Africa: From gene discovery to transgene expression |
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Sweby, D. L. Potier, B. A. M. Snyman, S. J. |
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2024-07-04T03:08:51.323Z |
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| score |
7.3993597 |