Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements

Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to...
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Autor*in:	da Silva, Danielle C. [verfasserIn] Visentainer, Jesuí V. de Souza, Nilson E. Oliveira, Cláudio C.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Water-soluble vitamins analysis Micellar electrokinetic chromatography Green chemistry

Anmerkung:	© Springer Science+Business Media New York 2013

Übergeordnetes Werk:	Enthalten in: Food analytical methods - New York, NY : Springer, 2008, 6(2013), 6 vom: 01. März, Seite 1592-1606
Übergeordnetes Werk:	volume:6 ; year:2013 ; number:6 ; day:01 ; month:03 ; pages:1592-1606

Links:	Volltext

DOI / URN:	10.1007/s12161-013-9576-x

Katalog-ID:	SPR025085271

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520			\|a Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods.
650		4	\|a Water-soluble vitamins analysis \|7 (dpeaa)DE-He213
650		4	\|a Micellar electrokinetic chromatography \|7 (dpeaa)DE-He213
650		4	\|a Green chemistry \|7 (dpeaa)DE-He213
700	1		\|a Visentainer, Jesuí V. \|4 aut
700	1		\|a de Souza, Nilson E. \|4 aut
700	1		\|a Oliveira, Cláudio C. \|4 aut
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912			\|a GBV_ILN_95
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912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
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912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
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912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4242
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912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
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952			\|d 6 \|j 2013 \|e 6 \|b 01 \|c 03 \|h 1592-1606

Indexfelder

author_variant	s d c d sdc sdcd j v v jv jvv s n e d sne sned c c o cc cco
matchkey_str	article:1936976X:2013----::ielrlcrkntchoaorpyehdodtriainfhtnaesl
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publishDate	2013
allfields	10.1007/s12161-013-9576-x doi (DE-627)SPR025085271 (SPR)s12161-013-9576-x-e DE-627 ger DE-627 rakwb eng da Silva, Danielle C. verfasserin aut Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2013 Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. Water-soluble vitamins analysis (dpeaa)DE-He213 Micellar electrokinetic chromatography (dpeaa)DE-He213 Green chemistry (dpeaa)DE-He213 Visentainer, Jesuí V. aut de Souza, Nilson E. aut Oliveira, Cláudio C. aut Enthalten in Food analytical methods New York, NY : Springer, 2008 6(2013), 6 vom: 01. März, Seite 1592-1606 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:6 year:2013 number:6 day:01 month:03 pages:1592-1606 https://dx.doi.org/10.1007/s12161-013-9576-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 6 01 03 1592-1606
spelling	10.1007/s12161-013-9576-x doi (DE-627)SPR025085271 (SPR)s12161-013-9576-x-e DE-627 ger DE-627 rakwb eng da Silva, Danielle C. verfasserin aut Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2013 Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. Water-soluble vitamins analysis (dpeaa)DE-He213 Micellar electrokinetic chromatography (dpeaa)DE-He213 Green chemistry (dpeaa)DE-He213 Visentainer, Jesuí V. aut de Souza, Nilson E. aut Oliveira, Cláudio C. aut Enthalten in Food analytical methods New York, NY : Springer, 2008 6(2013), 6 vom: 01. März, Seite 1592-1606 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:6 year:2013 number:6 day:01 month:03 pages:1592-1606 https://dx.doi.org/10.1007/s12161-013-9576-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 6 01 03 1592-1606
allfields_unstemmed	10.1007/s12161-013-9576-x doi (DE-627)SPR025085271 (SPR)s12161-013-9576-x-e DE-627 ger DE-627 rakwb eng da Silva, Danielle C. verfasserin aut Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2013 Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. Water-soluble vitamins analysis (dpeaa)DE-He213 Micellar electrokinetic chromatography (dpeaa)DE-He213 Green chemistry (dpeaa)DE-He213 Visentainer, Jesuí V. aut de Souza, Nilson E. aut Oliveira, Cláudio C. aut Enthalten in Food analytical methods New York, NY : Springer, 2008 6(2013), 6 vom: 01. März, Seite 1592-1606 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:6 year:2013 number:6 day:01 month:03 pages:1592-1606 https://dx.doi.org/10.1007/s12161-013-9576-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 6 01 03 1592-1606
allfieldsGer	10.1007/s12161-013-9576-x doi (DE-627)SPR025085271 (SPR)s12161-013-9576-x-e DE-627 ger DE-627 rakwb eng da Silva, Danielle C. verfasserin aut Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2013 Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. Water-soluble vitamins analysis (dpeaa)DE-He213 Micellar electrokinetic chromatography (dpeaa)DE-He213 Green chemistry (dpeaa)DE-He213 Visentainer, Jesuí V. aut de Souza, Nilson E. aut Oliveira, Cláudio C. aut Enthalten in Food analytical methods New York, NY : Springer, 2008 6(2013), 6 vom: 01. März, Seite 1592-1606 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:6 year:2013 number:6 day:01 month:03 pages:1592-1606 https://dx.doi.org/10.1007/s12161-013-9576-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 6 01 03 1592-1606
allfieldsSound	10.1007/s12161-013-9576-x doi (DE-627)SPR025085271 (SPR)s12161-013-9576-x-e DE-627 ger DE-627 rakwb eng da Silva, Danielle C. verfasserin aut Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2013 Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. Water-soluble vitamins analysis (dpeaa)DE-He213 Micellar electrokinetic chromatography (dpeaa)DE-He213 Green chemistry (dpeaa)DE-He213 Visentainer, Jesuí V. aut de Souza, Nilson E. aut Oliveira, Cláudio C. aut Enthalten in Food analytical methods New York, NY : Springer, 2008 6(2013), 6 vom: 01. März, Seite 1592-1606 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:6 year:2013 number:6 day:01 month:03 pages:1592-1606 https://dx.doi.org/10.1007/s12161-013-9576-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 6 01 03 1592-1606
language	English
source	Enthalten in Food analytical methods 6(2013), 6 vom: 01. März, Seite 1592-1606 volume:6 year:2013 number:6 day:01 month:03 pages:1592-1606
sourceStr	Enthalten in Food analytical methods 6(2013), 6 vom: 01. März, Seite 1592-1606 volume:6 year:2013 number:6 day:01 month:03 pages:1592-1606
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Water-soluble vitamins analysis Micellar electrokinetic chromatography Green chemistry
isfreeaccess_bool	false
container_title	Food analytical methods
authorswithroles_txt_mv	da Silva, Danielle C. @@aut@@ Visentainer, Jesuí V. @@aut@@ de Souza, Nilson E. @@aut@@ Oliveira, Cláudio C. @@aut@@
publishDateDaySort_date	2013-03-01T00:00:00Z
hierarchy_top_id	566007320
id	SPR025085271
language_de	englisch
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The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. 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author	da Silva, Danielle C.
spellingShingle	da Silva, Danielle C. misc Water-soluble vitamins analysis misc Micellar electrokinetic chromatography misc Green chemistry Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements
authorStr	da Silva, Danielle C.
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topic_title	Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements Water-soluble vitamins analysis (dpeaa)DE-He213 Micellar electrokinetic chromatography (dpeaa)DE-He213 Green chemistry (dpeaa)DE-He213
topic	misc Water-soluble vitamins analysis misc Micellar electrokinetic chromatography misc Green chemistry
topic_unstemmed	misc Water-soluble vitamins analysis misc Micellar electrokinetic chromatography misc Green chemistry
topic_browse	misc Water-soluble vitamins analysis misc Micellar electrokinetic chromatography misc Green chemistry
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title	Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements
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title_full	Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements
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author_browse	da Silva, Danielle C. Visentainer, Jesuí V. de Souza, Nilson E. Oliveira, Cláudio C.
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author-letter	da Silva, Danielle C.
doi_str_mv	10.1007/s12161-013-9576-x
title_sort	micellar electrokinetic chromatography method for determination of the ten water-soluble vitamins in food supplements
title_auth	Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements
abstract	Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. © Springer Science+Business Media New York 2013
abstractGer	Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. © Springer Science+Business Media New York 2013
abstract_unstemmed	Abstract The separation and determination of the ten water-soluble vitamins by using capillary electrophoresis in the micellar electrokinetic chromatography in a single run are proposed. The method uses low toxicity and cost solvent (ethanol) as modifier of background electrolyte (BGE) attending to the Green Chemistry principles. The electrophoretic method uses 10.0 % (v/v) ethanol, 2.0 % (w/v) SDS, 0.02 mol $ L^{−1} $ borate at pH 8.70 as BGE. The standard and real sample solutions were injected in the eletrophoretic system by hydrodynamic injection under pressure of 0.80 psi for 8 s, and the separation was carried out in a fused silica capillary under a potential of 28 kV at 25 °C; the analytical signals were monitored at 214 nm. The analytical method is precise (r.s.d. < 6 %), accurate (better than 9 %), selective, sensitive, robust, simple, and presents high analytical frequency as ten water-soluble vitamins were separated in only 18 min, with migration times of 5.75 ± 0.02, 6.81 ± 0.02, 8.13 ± 0.04, 8.80 ± 0.07, 8.98 ± 0.06, 11.10 ± 0.08, 11.34 ± 0.05, 13.85 ± 0.15, 14.82 ± 0.04, and 17.85 ± 0.30 min. Detection and quantification limits of 0.34, 0.32, 0.27, 0.20, 2.50, 4.98, 4.92, 0.30, 0.86 and 0.28 mg $ L^{−1} $ and 1.02, 0.97, 0.83, 0.62, 7.56, 15.09, 14.91, 0.90, 2.59 and 0.83 mg $ L^{−1} $, for vitamins PP (nicotinamide), $ B_{12} $ (cyanocobalamin), $ B_{2} $ (riboflavin), $ B_{6} $ (pyridoxine), $ B_{8} $ (biotin), C (ascorbic acid), $ B_{5} $ (pantothenic acid), $ B_{3} $ (nicotinic acid), $ B_{1} $ (thiamine), and $ B_{9} $ (folic acid), respectively. Excellent recoveries (intra and inter-day) were obtained and, when the method was applied to food supplement analyses the results were in agreement with the conventional HPLC methods. © Springer Science+Business Media New York 2013
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container_issue	6
title_short	Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements
url	https://dx.doi.org/10.1007/s12161-013-9576-x
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author2	Visentainer, Jesuí V. de Souza, Nilson E. Oliveira, Cláudio C.
author2Str	Visentainer, Jesuí V. de Souza, Nilson E. Oliveira, Cláudio C.
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doi_str	10.1007/s12161-013-9576-x
up_date	2024-07-03T13:45:14.498Z
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Micellar Electrokinetic Chromatography Method for Determination of the Ten Water-Soluble Vitamins in Food Supplements

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