Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo
Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL)...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kumano, Isao [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Anmerkung: |
© Biomedical Engineering Society 2011 |
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| Übergeordnetes Werk: |
Enthalten in: Cellular and molecular bioengineering - New Yok, NY [u.a.] : Springer, 2008, 4(2011), 3 vom: 30. Apr., Seite 476-483 |
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| Übergeordnetes Werk: |
volume:4 ; year:2011 ; number:3 ; day:30 ; month:04 ; pages:476-483 |
| Links: |
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| DOI / URN: |
10.1007/s12195-011-0172-0 |
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| Katalog-ID: |
SPR02518332X |
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| 100 | 1 | |a Kumano, Isao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo |
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| 520 | |a Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. | ||
| 650 | 4 | |a Tumor cell transplantation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cell carrier |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cell/F/P MP aggregate |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Fragmin/protamine microparticles |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Tumor growth |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Kishimoto, Satoko |4 aut | |
| 700 | 1 | |a Nakamura, Shingo |4 aut | |
| 700 | 1 | |a Hattori, Hidemi |4 aut | |
| 700 | 1 | |a Tanaka, Yoshihiro |4 aut | |
| 700 | 1 | |a Nakata, Mitsuhiro |4 aut | |
| 700 | 1 | |a Sato, Toshinori |4 aut | |
| 700 | 1 | |a Fujita, Masanori |4 aut | |
| 700 | 1 | |a Maehara, Tadaaki |4 aut | |
| 700 | 1 | |a Ishihara, Masayuki |4 aut | |
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| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
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| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
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10.1007/s12195-011-0172-0 doi (DE-627)SPR02518332X (SPR)s12195-011-0172-0-e DE-627 ger DE-627 rakwb eng Kumano, Isao verfasserin aut Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Biomedical Engineering Society 2011 Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. Tumor cell transplantation (dpeaa)DE-He213 Cell carrier (dpeaa)DE-He213 Cell/F/P MP aggregate (dpeaa)DE-He213 Fragmin/protamine microparticles (dpeaa)DE-He213 Tumor growth (dpeaa)DE-He213 Kishimoto, Satoko aut Nakamura, Shingo aut Hattori, Hidemi aut Tanaka, Yoshihiro aut Nakata, Mitsuhiro aut Sato, Toshinori aut Fujita, Masanori aut Maehara, Tadaaki aut Ishihara, Masayuki aut Enthalten in Cellular and molecular bioengineering New Yok, NY [u.a.] : Springer, 2008 4(2011), 3 vom: 30. Apr., Seite 476-483 (DE-627)560179030 (DE-600)2416037-4 1865-5033 nnns volume:4 year:2011 number:3 day:30 month:04 pages:476-483 https://dx.doi.org/10.1007/s12195-011-0172-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 4 2011 3 30 04 476-483 |
| spelling |
10.1007/s12195-011-0172-0 doi (DE-627)SPR02518332X (SPR)s12195-011-0172-0-e DE-627 ger DE-627 rakwb eng Kumano, Isao verfasserin aut Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Biomedical Engineering Society 2011 Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. Tumor cell transplantation (dpeaa)DE-He213 Cell carrier (dpeaa)DE-He213 Cell/F/P MP aggregate (dpeaa)DE-He213 Fragmin/protamine microparticles (dpeaa)DE-He213 Tumor growth (dpeaa)DE-He213 Kishimoto, Satoko aut Nakamura, Shingo aut Hattori, Hidemi aut Tanaka, Yoshihiro aut Nakata, Mitsuhiro aut Sato, Toshinori aut Fujita, Masanori aut Maehara, Tadaaki aut Ishihara, Masayuki aut Enthalten in Cellular and molecular bioengineering New Yok, NY [u.a.] : Springer, 2008 4(2011), 3 vom: 30. Apr., Seite 476-483 (DE-627)560179030 (DE-600)2416037-4 1865-5033 nnns volume:4 year:2011 number:3 day:30 month:04 pages:476-483 https://dx.doi.org/10.1007/s12195-011-0172-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 4 2011 3 30 04 476-483 |
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10.1007/s12195-011-0172-0 doi (DE-627)SPR02518332X (SPR)s12195-011-0172-0-e DE-627 ger DE-627 rakwb eng Kumano, Isao verfasserin aut Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Biomedical Engineering Society 2011 Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. Tumor cell transplantation (dpeaa)DE-He213 Cell carrier (dpeaa)DE-He213 Cell/F/P MP aggregate (dpeaa)DE-He213 Fragmin/protamine microparticles (dpeaa)DE-He213 Tumor growth (dpeaa)DE-He213 Kishimoto, Satoko aut Nakamura, Shingo aut Hattori, Hidemi aut Tanaka, Yoshihiro aut Nakata, Mitsuhiro aut Sato, Toshinori aut Fujita, Masanori aut Maehara, Tadaaki aut Ishihara, Masayuki aut Enthalten in Cellular and molecular bioengineering New Yok, NY [u.a.] : Springer, 2008 4(2011), 3 vom: 30. Apr., Seite 476-483 (DE-627)560179030 (DE-600)2416037-4 1865-5033 nnns volume:4 year:2011 number:3 day:30 month:04 pages:476-483 https://dx.doi.org/10.1007/s12195-011-0172-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 4 2011 3 30 04 476-483 |
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10.1007/s12195-011-0172-0 doi (DE-627)SPR02518332X (SPR)s12195-011-0172-0-e DE-627 ger DE-627 rakwb eng Kumano, Isao verfasserin aut Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Biomedical Engineering Society 2011 Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. Tumor cell transplantation (dpeaa)DE-He213 Cell carrier (dpeaa)DE-He213 Cell/F/P MP aggregate (dpeaa)DE-He213 Fragmin/protamine microparticles (dpeaa)DE-He213 Tumor growth (dpeaa)DE-He213 Kishimoto, Satoko aut Nakamura, Shingo aut Hattori, Hidemi aut Tanaka, Yoshihiro aut Nakata, Mitsuhiro aut Sato, Toshinori aut Fujita, Masanori aut Maehara, Tadaaki aut Ishihara, Masayuki aut Enthalten in Cellular and molecular bioengineering New Yok, NY [u.a.] : Springer, 2008 4(2011), 3 vom: 30. Apr., Seite 476-483 (DE-627)560179030 (DE-600)2416037-4 1865-5033 nnns volume:4 year:2011 number:3 day:30 month:04 pages:476-483 https://dx.doi.org/10.1007/s12195-011-0172-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 4 2011 3 30 04 476-483 |
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Enthalten in Cellular and molecular bioengineering 4(2011), 3 vom: 30. Apr., Seite 476-483 volume:4 year:2011 number:3 day:30 month:04 pages:476-483 |
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Enthalten in Cellular and molecular bioengineering 4(2011), 3 vom: 30. Apr., Seite 476-483 volume:4 year:2011 number:3 day:30 month:04 pages:476-483 |
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Kumano, Isao @@aut@@ Kishimoto, Satoko @@aut@@ Nakamura, Shingo @@aut@@ Hattori, Hidemi @@aut@@ Tanaka, Yoshihiro @@aut@@ Nakata, Mitsuhiro @@aut@@ Sato, Toshinori @@aut@@ Fujita, Masanori @@aut@@ Maehara, Tadaaki @@aut@@ Ishihara, Masayuki @@aut@@ |
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Kumano, Isao |
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Kumano, Isao misc Tumor cell transplantation misc Cell carrier misc Cell/F/P MP aggregate misc Fragmin/protamine microparticles misc Tumor growth Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo |
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Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo Tumor cell transplantation (dpeaa)DE-He213 Cell carrier (dpeaa)DE-He213 Cell/F/P MP aggregate (dpeaa)DE-He213 Fragmin/protamine microparticles (dpeaa)DE-He213 Tumor growth (dpeaa)DE-He213 |
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misc Tumor cell transplantation misc Cell carrier misc Cell/F/P MP aggregate misc Fragmin/protamine microparticles misc Tumor growth |
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Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo |
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Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo |
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Kumano, Isao Kishimoto, Satoko Nakamura, Shingo Hattori, Hidemi Tanaka, Yoshihiro Nakata, Mitsuhiro Sato, Toshinori Fujita, Masanori Maehara, Tadaaki Ishihara, Masayuki |
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fragmin/protamine microparticles (f/p mps) as cell carriers enhance the formation and growth of tumors in vivo |
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Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo |
| abstract |
Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. © Biomedical Engineering Society 2011 |
| abstractGer |
Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. © Biomedical Engineering Society 2011 |
| abstract_unstemmed |
Abstract Mixing a low-molecular-weight heparin (e.g., fragmin) with protamine results in the formation of water-insoluble fragmin/protamine microparticles (F/P MPs) approximately 0.5–3 μm in diameter. In this study, we investigated the ability of F/P MPs to improve the viability of Lewis lung (3LL) cancer cells, B16 (B16) melanoma cells, and a human hepatoma (Huh7) cell line in a suspension culture, and the ability of F/P MPs to enhance tumor take rate and growth in vivo. F/P MPs rapidly bound to the surface of the cells. The interaction of the cells with F/P MPs induced formations of the tumor cell/F/P MP aggregates and maintained the viability of those cells in suspension for at least 3 days. The addition of F/P MPs with FGF-2 significantly enhanced the growth rate of the cells in a fetal bovine serum (FBS)-free medium. The tumor cell/F/P MP aggregates, which were subcutaneously injected into the backs of mice, significantly stimulated the formation and growth of subcutaneous tumors consisting of 3LL, B16, and Huh7 cells. Furthermore, the tumors produced by injection of 7.5 × $ 10^{5} $ Huh7 into nude mice did grow only with F/P MPs. Thus F/P MPs can utilize as cell carrier for tumor cell transplantation. © Biomedical Engineering Society 2011 |
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| container_issue |
3 |
| title_short |
Fragmin/Protamine Microparticles (F/P MPs) as Cell Carriers Enhance the Formation and Growth of Tumors In Vivo |
| url |
https://dx.doi.org/10.1007/s12195-011-0172-0 |
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| author2 |
Kishimoto, Satoko Nakamura, Shingo Hattori, Hidemi Tanaka, Yoshihiro Nakata, Mitsuhiro Sato, Toshinori Fujita, Masanori Maehara, Tadaaki Ishihara, Masayuki |
| author2Str |
Kishimoto, Satoko Nakamura, Shingo Hattori, Hidemi Tanaka, Yoshihiro Nakata, Mitsuhiro Sato, Toshinori Fujita, Masanori Maehara, Tadaaki Ishihara, Masayuki |
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2024-07-03T14:23:53.144Z |
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| score |
7.40176 |