A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan
Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Van Gansbeke, Bart [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC 2017 |
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| Übergeordnetes Werk: |
Enthalten in: Food analytical methods - New York, NY : Springer, 2008, 11(2017), 1 vom: 08. Juli, Seite 62-68 |
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| Übergeordnetes Werk: |
volume:11 ; year:2017 ; number:1 ; day:08 ; month:07 ; pages:62-68 |
| Links: |
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| DOI / URN: |
10.1007/s12161-017-0964-5 |
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| Katalog-ID: |
SPR025203584 |
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| 100 | 1 | |a Van Gansbeke, Bart |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan |
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| 520 | |a Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. | ||
| 650 | 4 | |a Marzipan |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Persipan |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Real-time PCR |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Apricot |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Adulteration |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Bény, Guido |4 aut | |
| 700 | 1 | |a De Loose, Marc |4 aut | |
| 700 | 1 | |a Taverniers, Isabel |4 aut | |
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10.1007/s12161-017-0964-5 doi (DE-627)SPR025203584 (SPR)s12161-017-0964-5-e DE-627 ger DE-627 rakwb eng Van Gansbeke, Bart verfasserin aut A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2017 Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. Marzipan (dpeaa)DE-He213 Persipan (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 Apricot (dpeaa)DE-He213 Adulteration (dpeaa)DE-He213 Bény, Guido aut De Loose, Marc aut Taverniers, Isabel aut Enthalten in Food analytical methods New York, NY : Springer, 2008 11(2017), 1 vom: 08. Juli, Seite 62-68 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:11 year:2017 number:1 day:08 month:07 pages:62-68 https://dx.doi.org/10.1007/s12161-017-0964-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2017 1 08 07 62-68 |
| spelling |
10.1007/s12161-017-0964-5 doi (DE-627)SPR025203584 (SPR)s12161-017-0964-5-e DE-627 ger DE-627 rakwb eng Van Gansbeke, Bart verfasserin aut A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2017 Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. Marzipan (dpeaa)DE-He213 Persipan (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 Apricot (dpeaa)DE-He213 Adulteration (dpeaa)DE-He213 Bény, Guido aut De Loose, Marc aut Taverniers, Isabel aut Enthalten in Food analytical methods New York, NY : Springer, 2008 11(2017), 1 vom: 08. Juli, Seite 62-68 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:11 year:2017 number:1 day:08 month:07 pages:62-68 https://dx.doi.org/10.1007/s12161-017-0964-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2017 1 08 07 62-68 |
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10.1007/s12161-017-0964-5 doi (DE-627)SPR025203584 (SPR)s12161-017-0964-5-e DE-627 ger DE-627 rakwb eng Van Gansbeke, Bart verfasserin aut A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2017 Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. Marzipan (dpeaa)DE-He213 Persipan (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 Apricot (dpeaa)DE-He213 Adulteration (dpeaa)DE-He213 Bény, Guido aut De Loose, Marc aut Taverniers, Isabel aut Enthalten in Food analytical methods New York, NY : Springer, 2008 11(2017), 1 vom: 08. Juli, Seite 62-68 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:11 year:2017 number:1 day:08 month:07 pages:62-68 https://dx.doi.org/10.1007/s12161-017-0964-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2017 1 08 07 62-68 |
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10.1007/s12161-017-0964-5 doi (DE-627)SPR025203584 (SPR)s12161-017-0964-5-e DE-627 ger DE-627 rakwb eng Van Gansbeke, Bart verfasserin aut A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2017 Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. Marzipan (dpeaa)DE-He213 Persipan (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 Apricot (dpeaa)DE-He213 Adulteration (dpeaa)DE-He213 Bény, Guido aut De Loose, Marc aut Taverniers, Isabel aut Enthalten in Food analytical methods New York, NY : Springer, 2008 11(2017), 1 vom: 08. Juli, Seite 62-68 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:11 year:2017 number:1 day:08 month:07 pages:62-68 https://dx.doi.org/10.1007/s12161-017-0964-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2017 1 08 07 62-68 |
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10.1007/s12161-017-0964-5 doi (DE-627)SPR025203584 (SPR)s12161-017-0964-5-e DE-627 ger DE-627 rakwb eng Van Gansbeke, Bart verfasserin aut A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2017 Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. Marzipan (dpeaa)DE-He213 Persipan (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 Apricot (dpeaa)DE-He213 Adulteration (dpeaa)DE-He213 Bény, Guido aut De Loose, Marc aut Taverniers, Isabel aut Enthalten in Food analytical methods New York, NY : Springer, 2008 11(2017), 1 vom: 08. Juli, Seite 62-68 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:11 year:2017 number:1 day:08 month:07 pages:62-68 https://dx.doi.org/10.1007/s12161-017-0964-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2017 1 08 07 62-68 |
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| author |
Van Gansbeke, Bart |
| spellingShingle |
Van Gansbeke, Bart misc Marzipan misc Persipan misc Real-time PCR misc Apricot misc Adulteration A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan |
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A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan Marzipan (dpeaa)DE-He213 Persipan (dpeaa)DE-He213 Real-time PCR (dpeaa)DE-He213 Apricot (dpeaa)DE-He213 Adulteration (dpeaa)DE-He213 |
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misc Marzipan misc Persipan misc Real-time PCR misc Apricot misc Adulteration |
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misc Marzipan misc Persipan misc Real-time PCR misc Apricot misc Adulteration |
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A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan |
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A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan |
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Van Gansbeke, Bart |
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Van Gansbeke, Bart Bény, Guido De Loose, Marc Taverniers, Isabel |
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taqman real-time pcr assay for apricot (prunus armeniaca) as an authenticity test for detection of traces of persipan in marzipan |
| title_auth |
A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan |
| abstract |
Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. © Springer Science+Business Media, LLC 2017 |
| abstractGer |
Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. © Springer Science+Business Media, LLC 2017 |
| abstract_unstemmed |
Abstract Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipid transfer protein (LTP), used as target sequence for a qPCR test, can be noticed. In this study, a TaqMan qPCR method for specific detection of apricot in marzipan was developed. After optimization, specificity and sensitivity tests were carried out on real samples and the limit of detection was determined. The TaqMan qPCR method showed high specificity for apricot and was able to detect as low as 1% persipan in marzipan. © Springer Science+Business Media, LLC 2017 |
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| title_short |
A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan |
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https://dx.doi.org/10.1007/s12161-017-0964-5 |
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Bény, Guido De Loose, Marc Taverniers, Isabel |
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| doi_str |
10.1007/s12161-017-0964-5 |
| up_date |
2024-07-03T14:32:13.982Z |
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| score |
7.4006405 |