Microfluidics in biology and genosensor construction
Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Peltek, S. E. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Schlagwörter: |
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| Anmerkung: |
© Pleiades Publishing, Ltd. 2008 |
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| Übergeordnetes Werk: |
Enthalten in: Nanotechnologies in Russia - [Moskau] : Pleiades Publishing, 2008, 3(2008), 9-10 vom: Okt., Seite 622-632 |
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| Übergeordnetes Werk: |
volume:3 ; year:2008 ; number:9-10 ; month:10 ; pages:622-632 |
| Links: |
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| DOI / URN: |
10.1134/S1995078008090127 |
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| Katalog-ID: |
SPR025214136 |
|---|
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| 100 | 1 | |a Peltek, S. E. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Microfluidics in biology and genosensor construction |
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| 520 | |a Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. | ||
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| 700 | 1 | |a Goryachkovskaya, T. N. |4 aut | |
| 700 | 1 | |a Popik, V. M. |4 aut | |
| 700 | 1 | |a Pindyurin, V. F. |4 aut | |
| 700 | 1 | |a Eliseev, V. S. |4 aut | |
| 700 | 1 | |a Gol’denberg, B. G. |4 aut | |
| 700 | 1 | |a Shcheglov, M. A. |4 aut | |
| 700 | 1 | |a Tikunova, N. V. |4 aut | |
| 700 | 1 | |a Khlebodarova, T. M. |4 aut | |
| 700 | 1 | |a Rubtsov, N. B. |4 aut | |
| 700 | 1 | |a Kulipanov, G. N. |4 aut | |
| 700 | 1 | |a Kolchanov, N. A. |4 aut | |
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10.1134/S1995078008090127 doi (DE-627)SPR025214136 (SPR)S1995078008090127-e DE-627 ger DE-627 rakwb eng Peltek, S. E. verfasserin aut Microfluidics in biology and genosensor construction 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2008 Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. PMMA (dpeaa)DE-He213 Damage Factor (dpeaa)DE-He213 Bioanalytical System (dpeaa)DE-He213 Synchrotron Radiation Beam (dpeaa)DE-He213 katG Gene (dpeaa)DE-He213 Goryachkovskaya, T. N. aut Popik, V. M. aut Pindyurin, V. F. aut Eliseev, V. S. aut Gol’denberg, B. G. aut Shcheglov, M. A. aut Tikunova, N. V. aut Khlebodarova, T. M. aut Rubtsov, N. B. aut Kulipanov, G. N. aut Kolchanov, N. A. aut Enthalten in Nanotechnologies in Russia [Moskau] : Pleiades Publishing, 2008 3(2008), 9-10 vom: Okt., Seite 622-632 (DE-627)565518887 (DE-600)2424432-6 1995-0799 nnns volume:3 year:2008 number:9-10 month:10 pages:622-632 https://dx.doi.org/10.1134/S1995078008090127 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 3 2008 9-10 10 622-632 |
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10.1134/S1995078008090127 doi (DE-627)SPR025214136 (SPR)S1995078008090127-e DE-627 ger DE-627 rakwb eng Peltek, S. E. verfasserin aut Microfluidics in biology and genosensor construction 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2008 Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. PMMA (dpeaa)DE-He213 Damage Factor (dpeaa)DE-He213 Bioanalytical System (dpeaa)DE-He213 Synchrotron Radiation Beam (dpeaa)DE-He213 katG Gene (dpeaa)DE-He213 Goryachkovskaya, T. N. aut Popik, V. M. aut Pindyurin, V. F. aut Eliseev, V. S. aut Gol’denberg, B. G. aut Shcheglov, M. A. aut Tikunova, N. V. aut Khlebodarova, T. M. aut Rubtsov, N. B. aut Kulipanov, G. N. aut Kolchanov, N. A. aut Enthalten in Nanotechnologies in Russia [Moskau] : Pleiades Publishing, 2008 3(2008), 9-10 vom: Okt., Seite 622-632 (DE-627)565518887 (DE-600)2424432-6 1995-0799 nnns volume:3 year:2008 number:9-10 month:10 pages:622-632 https://dx.doi.org/10.1134/S1995078008090127 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 3 2008 9-10 10 622-632 |
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10.1134/S1995078008090127 doi (DE-627)SPR025214136 (SPR)S1995078008090127-e DE-627 ger DE-627 rakwb eng Peltek, S. E. verfasserin aut Microfluidics in biology and genosensor construction 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2008 Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. PMMA (dpeaa)DE-He213 Damage Factor (dpeaa)DE-He213 Bioanalytical System (dpeaa)DE-He213 Synchrotron Radiation Beam (dpeaa)DE-He213 katG Gene (dpeaa)DE-He213 Goryachkovskaya, T. N. aut Popik, V. M. aut Pindyurin, V. F. aut Eliseev, V. S. aut Gol’denberg, B. G. aut Shcheglov, M. A. aut Tikunova, N. V. aut Khlebodarova, T. M. aut Rubtsov, N. B. aut Kulipanov, G. N. aut Kolchanov, N. A. aut Enthalten in Nanotechnologies in Russia [Moskau] : Pleiades Publishing, 2008 3(2008), 9-10 vom: Okt., Seite 622-632 (DE-627)565518887 (DE-600)2424432-6 1995-0799 nnns volume:3 year:2008 number:9-10 month:10 pages:622-632 https://dx.doi.org/10.1134/S1995078008090127 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 3 2008 9-10 10 622-632 |
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10.1134/S1995078008090127 doi (DE-627)SPR025214136 (SPR)S1995078008090127-e DE-627 ger DE-627 rakwb eng Peltek, S. E. verfasserin aut Microfluidics in biology and genosensor construction 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2008 Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. PMMA (dpeaa)DE-He213 Damage Factor (dpeaa)DE-He213 Bioanalytical System (dpeaa)DE-He213 Synchrotron Radiation Beam (dpeaa)DE-He213 katG Gene (dpeaa)DE-He213 Goryachkovskaya, T. N. aut Popik, V. M. aut Pindyurin, V. F. aut Eliseev, V. S. aut Gol’denberg, B. G. aut Shcheglov, M. A. aut Tikunova, N. V. aut Khlebodarova, T. M. aut Rubtsov, N. B. aut Kulipanov, G. N. aut Kolchanov, N. A. aut Enthalten in Nanotechnologies in Russia [Moskau] : Pleiades Publishing, 2008 3(2008), 9-10 vom: Okt., Seite 622-632 (DE-627)565518887 (DE-600)2424432-6 1995-0799 nnns volume:3 year:2008 number:9-10 month:10 pages:622-632 https://dx.doi.org/10.1134/S1995078008090127 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 3 2008 9-10 10 622-632 |
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10.1134/S1995078008090127 doi (DE-627)SPR025214136 (SPR)S1995078008090127-e DE-627 ger DE-627 rakwb eng Peltek, S. E. verfasserin aut Microfluidics in biology and genosensor construction 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Pleiades Publishing, Ltd. 2008 Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. PMMA (dpeaa)DE-He213 Damage Factor (dpeaa)DE-He213 Bioanalytical System (dpeaa)DE-He213 Synchrotron Radiation Beam (dpeaa)DE-He213 katG Gene (dpeaa)DE-He213 Goryachkovskaya, T. N. aut Popik, V. M. aut Pindyurin, V. F. aut Eliseev, V. S. aut Gol’denberg, B. G. aut Shcheglov, M. A. aut Tikunova, N. V. aut Khlebodarova, T. M. aut Rubtsov, N. B. aut Kulipanov, G. N. aut Kolchanov, N. A. aut Enthalten in Nanotechnologies in Russia [Moskau] : Pleiades Publishing, 2008 3(2008), 9-10 vom: Okt., Seite 622-632 (DE-627)565518887 (DE-600)2424432-6 1995-0799 nnns volume:3 year:2008 number:9-10 month:10 pages:622-632 https://dx.doi.org/10.1134/S1995078008090127 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 3 2008 9-10 10 622-632 |
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Enthalten in Nanotechnologies in Russia 3(2008), 9-10 vom: Okt., Seite 622-632 volume:3 year:2008 number:9-10 month:10 pages:622-632 |
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Peltek, S. E. @@aut@@ Goryachkovskaya, T. N. @@aut@@ Popik, V. M. @@aut@@ Pindyurin, V. F. @@aut@@ Eliseev, V. S. @@aut@@ Gol’denberg, B. G. @@aut@@ Shcheglov, M. A. @@aut@@ Tikunova, N. V. @@aut@@ Khlebodarova, T. M. @@aut@@ Rubtsov, N. B. @@aut@@ Kulipanov, G. N. @@aut@@ Kolchanov, N. A. @@aut@@ |
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Peltek, S. E. |
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Peltek, S. E. Goryachkovskaya, T. N. Popik, V. M. Pindyurin, V. F. Eliseev, V. S. Gol’denberg, B. G. Shcheglov, M. A. Tikunova, N. V. Khlebodarova, T. M. Rubtsov, N. B. Kulipanov, G. N. Kolchanov, N. A. |
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microfluidics in biology and genosensor construction |
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Microfluidics in biology and genosensor construction |
| abstract |
Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. © Pleiades Publishing, Ltd. 2008 |
| abstractGer |
Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. © Pleiades Publishing, Ltd. 2008 |
| abstract_unstemmed |
Abstract We describe key stages in the development of a genosensor-based bioanalytical complex capable of functioning in a micro/nanofluidic system (MNFS), which is intended for detecting damaging agents in liquid media. Using the LIGA technology, we have developed an MNFS with a channel width of a few microns or more. Methods of (i) processing microfluid channels for positioning bacterial cells-genosensors and (ii) detecting fluorescent signals in a microfluid module are developed. A search for promoters ensuring the response of genosensor structures to a broad range of damaging factors of the external environment is carried out using a bioinformatics approach (based on GenSensor database). An experimental genosensor has been constructed based on the dps gene promoter of E. coli and the pRS-GFPvaa vector plasmid. It is shown that an E. coli/pDps-gfp genosensor in a microfluid module responds by GFPvaa protein synthesis to the presence of oxidative stress agents and agents capable of penetrating membranes in the same range of concentrations as those outside the module. The results of our investigation show that the proposed interdisciplinary approach can be used for obtaining bacterial cells-genosensors capable of functioning in MNFSs and detecting damaging factors of the external environment. © Pleiades Publishing, Ltd. 2008 |
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| title_short |
Microfluidics in biology and genosensor construction |
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| author2 |
Goryachkovskaya, T. N. Popik, V. M. Pindyurin, V. F. Eliseev, V. S. Gol’denberg, B. G. Shcheglov, M. A. Tikunova, N. V. Khlebodarova, T. M. Rubtsov, N. B. Kulipanov, G. N. Kolchanov, N. A. |
| author2Str |
Goryachkovskaya, T. N. Popik, V. M. Pindyurin, V. F. Eliseev, V. S. Gol’denberg, B. G. Shcheglov, M. A. Tikunova, N. V. Khlebodarova, T. M. Rubtsov, N. B. Kulipanov, G. N. Kolchanov, N. A. |
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| up_date |
2024-07-03T14:36:03.595Z |
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| score |
7.400193 |