Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products
Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xue, Yu [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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| Übergeordnetes Werk: |
Enthalten in: Food analytical methods - New York, NY : Springer, 2008, 12(2019), 5 vom: 08. Feb., Seite 1197-1207 |
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| Übergeordnetes Werk: |
volume:12 ; year:2019 ; number:5 ; day:08 ; month:02 ; pages:1197-1207 |
| Links: |
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| DOI / URN: |
10.1007/s12161-019-01457-z |
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| Katalog-ID: |
SPR025260375 |
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| 245 | 1 | 0 | |a Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products |
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| 520 | |a Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. | ||
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| 650 | 4 | |a Immunomagnetic beads |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Electrochemical detection |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Dairy products |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Jiang, Dong-lei |4 aut | |
| 700 | 1 | |a Hu, Qin |4 aut | |
| 700 | 1 | |a Rao, Sheng-qi |4 aut | |
| 700 | 1 | |a Gao, Lu |4 aut | |
| 700 | 1 | |a Yang, Zhen-quan |4 aut | |
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| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
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10.1007/s12161-019-01457-z doi (DE-627)SPR025260375 (SPR)s12161-019-01457-z-e DE-627 ger DE-627 rakwb eng Xue, Yu verfasserin aut Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. GG (dpeaa)DE-He213 Immunomagnetic beads (dpeaa)DE-He213 Electrochemical detection (dpeaa)DE-He213 Dairy products (dpeaa)DE-He213 Jiang, Dong-lei aut Hu, Qin aut Rao, Sheng-qi aut Gao, Lu aut Yang, Zhen-quan aut Enthalten in Food analytical methods New York, NY : Springer, 2008 12(2019), 5 vom: 08. Feb., Seite 1197-1207 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:12 year:2019 number:5 day:08 month:02 pages:1197-1207 https://dx.doi.org/10.1007/s12161-019-01457-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2019 5 08 02 1197-1207 |
| spelling |
10.1007/s12161-019-01457-z doi (DE-627)SPR025260375 (SPR)s12161-019-01457-z-e DE-627 ger DE-627 rakwb eng Xue, Yu verfasserin aut Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. GG (dpeaa)DE-He213 Immunomagnetic beads (dpeaa)DE-He213 Electrochemical detection (dpeaa)DE-He213 Dairy products (dpeaa)DE-He213 Jiang, Dong-lei aut Hu, Qin aut Rao, Sheng-qi aut Gao, Lu aut Yang, Zhen-quan aut Enthalten in Food analytical methods New York, NY : Springer, 2008 12(2019), 5 vom: 08. Feb., Seite 1197-1207 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:12 year:2019 number:5 day:08 month:02 pages:1197-1207 https://dx.doi.org/10.1007/s12161-019-01457-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2019 5 08 02 1197-1207 |
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10.1007/s12161-019-01457-z doi (DE-627)SPR025260375 (SPR)s12161-019-01457-z-e DE-627 ger DE-627 rakwb eng Xue, Yu verfasserin aut Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. GG (dpeaa)DE-He213 Immunomagnetic beads (dpeaa)DE-He213 Electrochemical detection (dpeaa)DE-He213 Dairy products (dpeaa)DE-He213 Jiang, Dong-lei aut Hu, Qin aut Rao, Sheng-qi aut Gao, Lu aut Yang, Zhen-quan aut Enthalten in Food analytical methods New York, NY : Springer, 2008 12(2019), 5 vom: 08. Feb., Seite 1197-1207 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:12 year:2019 number:5 day:08 month:02 pages:1197-1207 https://dx.doi.org/10.1007/s12161-019-01457-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2019 5 08 02 1197-1207 |
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10.1007/s12161-019-01457-z doi (DE-627)SPR025260375 (SPR)s12161-019-01457-z-e DE-627 ger DE-627 rakwb eng Xue, Yu verfasserin aut Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. GG (dpeaa)DE-He213 Immunomagnetic beads (dpeaa)DE-He213 Electrochemical detection (dpeaa)DE-He213 Dairy products (dpeaa)DE-He213 Jiang, Dong-lei aut Hu, Qin aut Rao, Sheng-qi aut Gao, Lu aut Yang, Zhen-quan aut Enthalten in Food analytical methods New York, NY : Springer, 2008 12(2019), 5 vom: 08. Feb., Seite 1197-1207 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:12 year:2019 number:5 day:08 month:02 pages:1197-1207 https://dx.doi.org/10.1007/s12161-019-01457-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2019 5 08 02 1197-1207 |
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10.1007/s12161-019-01457-z doi (DE-627)SPR025260375 (SPR)s12161-019-01457-z-e DE-627 ger DE-627 rakwb eng Xue, Yu verfasserin aut Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. GG (dpeaa)DE-He213 Immunomagnetic beads (dpeaa)DE-He213 Electrochemical detection (dpeaa)DE-He213 Dairy products (dpeaa)DE-He213 Jiang, Dong-lei aut Hu, Qin aut Rao, Sheng-qi aut Gao, Lu aut Yang, Zhen-quan aut Enthalten in Food analytical methods New York, NY : Springer, 2008 12(2019), 5 vom: 08. Feb., Seite 1197-1207 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:12 year:2019 number:5 day:08 month:02 pages:1197-1207 https://dx.doi.org/10.1007/s12161-019-01457-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 12 2019 5 08 02 1197-1207 |
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Xue, Yu @@aut@@ Jiang, Dong-lei @@aut@@ Hu, Qin @@aut@@ Rao, Sheng-qi @@aut@@ Gao, Lu @@aut@@ Yang, Zhen-quan @@aut@@ |
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In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. 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Xue, Yu |
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Xue, Yu misc GG misc Immunomagnetic beads misc Electrochemical detection misc Dairy products Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products |
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Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products GG (dpeaa)DE-He213 Immunomagnetic beads (dpeaa)DE-He213 Electrochemical detection (dpeaa)DE-He213 Dairy products (dpeaa)DE-He213 |
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misc GG misc Immunomagnetic beads misc Electrochemical detection misc Dairy products |
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misc GG misc Immunomagnetic beads misc Electrochemical detection misc Dairy products |
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Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products |
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Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products |
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Xue, Yu Jiang, Dong-lei Hu, Qin Rao, Sheng-qi Gao, Lu Yang, Zhen-quan |
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electrochemical magnetic bead-based immunosensor for rapid and quantitative detection of probiotic lactobacillus rhamnosus in dairy products |
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Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products |
| abstract |
Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. © Springer Science+Business Media, LLC, part of Springer Nature 2019 |
| abstractGer |
Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. © Springer Science+Business Media, LLC, part of Springer Nature 2019 |
| abstract_unstemmed |
Abstract Rapid and quantitative detection of probiotic Lactobacillus rhamnosus at the strain level is important for quality control of probiotic products. In this study, an electrochemical magnetic bead-based immunosensor (EMBI) was developed for the specific quantification of probiotic L. rhamnosus strain GG (LGG) in dairy products. Magnetic beads coupled with a specific antibody against the pilus subunit SpaA of LGG (Ab-SpaA) were prepared to selectively capture LGG from the background, which were then detected using horseradish peroxidase-labeled Ab-SpaA. The resultant sandwich-type immunocomplexes were separated by magnetic force and detected by measuring current signals using a magnetic glassy carbon electrode (MGCE) and the hydroquinone (HQ)/$ H_{2} %$ O_{2} $ system. Under optimal experimental conditions, the developed EMBI showed a linear relationship between the peak current and the logarithmic value of LGG concentration ranging from 2.56 × $ 10^{3} $ to 2.56 × $ 10^{7} $ CFU $ mL^{−1} $ with a detection limit of 22 CFU $ mL^{−1} $. EMBI detection is LGG specific, and no cross-reaction was observed for tested strains of other lactic acid bacterial species. The EMBI was successfully applied for LGG determination in commercial milk, yogurt, milk beverage products, and spiked dairy samples, with a recovery rate in the range of 91.74–108.67%. The entire detection process could be completed within 3 h. The proposed biosensor shows low-cost, rapid response, and high sensitivity and specificity and could be a promising technique for quality detection and functional evaluation of probiotic products containing LGG. © Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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5 |
| title_short |
Electrochemical Magnetic Bead-Based Immunosensor for Rapid and Quantitative Detection of Probiotic Lactobacillus rhamnosus in Dairy Products |
| url |
https://dx.doi.org/10.1007/s12161-019-01457-z |
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| author2 |
Jiang, Dong-lei Hu, Qin Rao, Sheng-qi Gao, Lu Yang, Zhen-quan |
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| doi_str |
10.1007/s12161-019-01457-z |
| up_date |
2024-07-03T14:52:54.214Z |
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| score |
7.401781 |