Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing
Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios o...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Varshosaz, Jaleh [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Schlagwörter: |
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| Anmerkung: |
© The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 |
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| Übergeordnetes Werk: |
Enthalten in: Fibers and polymers - Seoul : The Korean Fiber Society, 2000, 18(2017), 11 vom: Nov., Seite 2125-2134 |
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| Übergeordnetes Werk: |
volume:18 ; year:2017 ; number:11 ; month:11 ; pages:2125-2134 |
| Links: |
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| DOI / URN: |
10.1007/s12221-017-7438-7 |
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| Katalog-ID: |
SPR025437968 |
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| 520 | |a Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). | ||
| 650 | 4 | |a Montelukast |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Nanofibers |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Poly(methyl vinyl ether-co-maleic acid) |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Wound dressing |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PLGA |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Jahanian, Ali |4 aut | |
| 700 | 1 | |a Maktoobian, Masoud |4 aut | |
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10.1007/s12221-017-7438-7 doi (DE-627)SPR025437968 (SPR)s12221-017-7438-7-e DE-627 ger DE-627 rakwb eng Varshosaz, Jaleh verfasserin aut Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). Montelukast (dpeaa)DE-He213 Nanofibers (dpeaa)DE-He213 Poly(methyl vinyl ether-co-maleic acid) (dpeaa)DE-He213 Wound dressing (dpeaa)DE-He213 PLGA (dpeaa)DE-He213 Jahanian, Ali aut Maktoobian, Masoud aut Enthalten in Fibers and polymers Seoul : The Korean Fiber Society, 2000 18(2017), 11 vom: Nov., Seite 2125-2134 (DE-627)565516485 (DE-600)2424081-3 1875-0052 nnns volume:18 year:2017 number:11 month:11 pages:2125-2134 https://dx.doi.org/10.1007/s12221-017-7438-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 18 2017 11 11 2125-2134 |
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10.1007/s12221-017-7438-7 doi (DE-627)SPR025437968 (SPR)s12221-017-7438-7-e DE-627 ger DE-627 rakwb eng Varshosaz, Jaleh verfasserin aut Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). Montelukast (dpeaa)DE-He213 Nanofibers (dpeaa)DE-He213 Poly(methyl vinyl ether-co-maleic acid) (dpeaa)DE-He213 Wound dressing (dpeaa)DE-He213 PLGA (dpeaa)DE-He213 Jahanian, Ali aut Maktoobian, Masoud aut Enthalten in Fibers and polymers Seoul : The Korean Fiber Society, 2000 18(2017), 11 vom: Nov., Seite 2125-2134 (DE-627)565516485 (DE-600)2424081-3 1875-0052 nnns volume:18 year:2017 number:11 month:11 pages:2125-2134 https://dx.doi.org/10.1007/s12221-017-7438-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 18 2017 11 11 2125-2134 |
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10.1007/s12221-017-7438-7 doi (DE-627)SPR025437968 (SPR)s12221-017-7438-7-e DE-627 ger DE-627 rakwb eng Varshosaz, Jaleh verfasserin aut Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). Montelukast (dpeaa)DE-He213 Nanofibers (dpeaa)DE-He213 Poly(methyl vinyl ether-co-maleic acid) (dpeaa)DE-He213 Wound dressing (dpeaa)DE-He213 PLGA (dpeaa)DE-He213 Jahanian, Ali aut Maktoobian, Masoud aut Enthalten in Fibers and polymers Seoul : The Korean Fiber Society, 2000 18(2017), 11 vom: Nov., Seite 2125-2134 (DE-627)565516485 (DE-600)2424081-3 1875-0052 nnns volume:18 year:2017 number:11 month:11 pages:2125-2134 https://dx.doi.org/10.1007/s12221-017-7438-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 18 2017 11 11 2125-2134 |
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10.1007/s12221-017-7438-7 doi (DE-627)SPR025437968 (SPR)s12221-017-7438-7-e DE-627 ger DE-627 rakwb eng Varshosaz, Jaleh verfasserin aut Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). Montelukast (dpeaa)DE-He213 Nanofibers (dpeaa)DE-He213 Poly(methyl vinyl ether-co-maleic acid) (dpeaa)DE-He213 Wound dressing (dpeaa)DE-He213 PLGA (dpeaa)DE-He213 Jahanian, Ali aut Maktoobian, Masoud aut Enthalten in Fibers and polymers Seoul : The Korean Fiber Society, 2000 18(2017), 11 vom: Nov., Seite 2125-2134 (DE-627)565516485 (DE-600)2424081-3 1875-0052 nnns volume:18 year:2017 number:11 month:11 pages:2125-2134 https://dx.doi.org/10.1007/s12221-017-7438-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 18 2017 11 11 2125-2134 |
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10.1007/s12221-017-7438-7 doi (DE-627)SPR025437968 (SPR)s12221-017-7438-7-e DE-627 ger DE-627 rakwb eng Varshosaz, Jaleh verfasserin aut Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). Montelukast (dpeaa)DE-He213 Nanofibers (dpeaa)DE-He213 Poly(methyl vinyl ether-co-maleic acid) (dpeaa)DE-He213 Wound dressing (dpeaa)DE-He213 PLGA (dpeaa)DE-He213 Jahanian, Ali aut Maktoobian, Masoud aut Enthalten in Fibers and polymers Seoul : The Korean Fiber Society, 2000 18(2017), 11 vom: Nov., Seite 2125-2134 (DE-627)565516485 (DE-600)2424081-3 1875-0052 nnns volume:18 year:2017 number:11 month:11 pages:2125-2134 https://dx.doi.org/10.1007/s12221-017-7438-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 18 2017 11 11 2125-2134 |
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Enthalten in Fibers and polymers 18(2017), 11 vom: Nov., Seite 2125-2134 volume:18 year:2017 number:11 month:11 pages:2125-2134 |
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Enthalten in Fibers and polymers 18(2017), 11 vom: Nov., Seite 2125-2134 volume:18 year:2017 number:11 month:11 pages:2125-2134 |
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Montelukast Nanofibers Poly(methyl vinyl ether-co-maleic acid) Wound dressing PLGA |
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Fibers and polymers |
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Varshosaz, Jaleh @@aut@@ Jahanian, Ali @@aut@@ Maktoobian, Masoud @@aut@@ |
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2017-11-01T00:00:00Z |
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Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. 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Varshosaz, Jaleh |
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Varshosaz, Jaleh misc Montelukast misc Nanofibers misc Poly(methyl vinyl ether-co-maleic acid) misc Wound dressing misc PLGA Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing |
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Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing Montelukast (dpeaa)DE-He213 Nanofibers (dpeaa)DE-He213 Poly(methyl vinyl ether-co-maleic acid) (dpeaa)DE-He213 Wound dressing (dpeaa)DE-He213 PLGA (dpeaa)DE-He213 |
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misc Montelukast misc Nanofibers misc Poly(methyl vinyl ether-co-maleic acid) misc Wound dressing misc PLGA |
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Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing |
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Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing |
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Varshosaz, Jaleh Jahanian, Ali Maktoobian, Masoud |
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montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing |
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Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing |
| abstract |
Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 |
| abstractGer |
Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 |
| abstract_unstemmed |
Abstract The aim of the present study was to prepare nanofibers loaded with montelukast, a cysteinyl leukotrienes (CysLTs) inhibitor, with anti-inflammatory properties effective on wound healing. Polymeric nanofibers containing montelukast were spun by electrospinning method using different ratios of the blend of two biodegradable polymers of poly(methyl vinyl etherco-maleic acid) (PMVEMA) and poly(lactic-co-glycolic acid) (PLGA) at the total polymer concentration of 37 %, the distance of the needle to rotating screen of 19 cm, the voltage of 12 Kv and the rate of injection of 0.2 ml/h. The ratio of two polymers in the blend and the concentration of montelukast were optimized based on the diameter of the nanofibers, drug loading percent and release efficiency by a full factorial design. The morphology, diameter and diameter distribution of the nanofibers were studied by scanning electron microscopy (SEM). Drug loading percent in the nanofibers was determined by extracting the loaded drug from a specific surface of the nanofibers which was subsequently analyzed spectrophotometrically. The drug release rate from the nanofibers was studied in phosphate buffer solution (pH 7.4) containing 0.5 % Tween 20 at predetermined time intervals until 10 days. The cytotoxicity of the designed nanofibers was evaluated on mouse fibroblast cells using trypan blue method, their platelet adherence property was quantified by measuring the lactate dehydrogenase (LDH) activity and confirmed by SEM micrographs. The optimized ratio of PLGA/PMVEMA was 3:1 with the total concentration of polymers as 37 % loaded with 30 % of montelukast produced nanofibers with a diameter of 157.6 nm, drug loading percent of 43.7 % and release efficiency of 75 % after 10 days. The cell viability was similar in nanofibers and the negative control group. The platelets adhesion to the nanofibers was more than the negative control group (p<0.05). © The Korean Fiber Society and Springer Science+Business Media B.V., part of Springer Nature 2017 |
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11 |
| title_short |
Montelukast incorporated poly(methyl vinyl ether-co-maleic acid)/poly(lactic-co-glycolic acid) electrospun nanofibers for wound dressing |
| url |
https://dx.doi.org/10.1007/s12221-017-7438-7 |
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Jahanian, Ali Maktoobian, Masoud |
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| doi_str |
10.1007/s12221-017-7438-7 |
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2024-07-03T15:59:44.809Z |
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| score |
7.3990545 |