Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada
Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Nanayakkara, Upeksha N. [verfasserIn] Singh, Mathuresh [verfasserIn] Pelletier, Yvan [verfasserIn] Nie, Xianzhou [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2012 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: American journal of potato research - New York, NY : Springer, 1923, 89(2012), 3 vom: 12. Mai, Seite 232-239 |
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| Übergeordnetes Werk: |
volume:89 ; year:2012 ; number:3 ; day:12 ; month:05 ; pages:232-239 |
| Links: |
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| DOI / URN: |
10.1007/s12230-012-9247-2 |
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| Katalog-ID: |
SPR025506218 |
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| 245 | 1 | 0 | |a Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada |
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| 520 | |a Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. | ||
| 650 | 4 | |a Potato |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Strain |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Population distribution |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Singh, Mathuresh |e verfasserin |4 aut | |
| 700 | 1 | |a Pelletier, Yvan |e verfasserin |4 aut | |
| 700 | 1 | |a Nie, Xianzhou |e verfasserin |4 aut | |
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10.1007/s12230-012-9247-2 doi (DE-627)SPR025506218 (SPR)s12230-012-9247-2-e DE-627 ger DE-627 rakwb eng 630 640 ASE Nanayakkara, Upeksha N. verfasserin aut Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. Potato (dpeaa)DE-He213 Strain (dpeaa)DE-He213 Population distribution (dpeaa)DE-He213 Singh, Mathuresh verfasserin aut Pelletier, Yvan verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in American journal of potato research New York, NY : Springer, 1923 89(2012), 3 vom: 12. Mai, Seite 232-239 (DE-627)54963410X (DE-600)2395546-6 1874-9380 nnns volume:89 year:2012 number:3 day:12 month:05 pages:232-239 https://dx.doi.org/10.1007/s12230-012-9247-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 89 2012 3 12 05 232-239 |
| spelling |
10.1007/s12230-012-9247-2 doi (DE-627)SPR025506218 (SPR)s12230-012-9247-2-e DE-627 ger DE-627 rakwb eng 630 640 ASE Nanayakkara, Upeksha N. verfasserin aut Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. Potato (dpeaa)DE-He213 Strain (dpeaa)DE-He213 Population distribution (dpeaa)DE-He213 Singh, Mathuresh verfasserin aut Pelletier, Yvan verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in American journal of potato research New York, NY : Springer, 1923 89(2012), 3 vom: 12. Mai, Seite 232-239 (DE-627)54963410X (DE-600)2395546-6 1874-9380 nnns volume:89 year:2012 number:3 day:12 month:05 pages:232-239 https://dx.doi.org/10.1007/s12230-012-9247-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 89 2012 3 12 05 232-239 |
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10.1007/s12230-012-9247-2 doi (DE-627)SPR025506218 (SPR)s12230-012-9247-2-e DE-627 ger DE-627 rakwb eng 630 640 ASE Nanayakkara, Upeksha N. verfasserin aut Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. Potato (dpeaa)DE-He213 Strain (dpeaa)DE-He213 Population distribution (dpeaa)DE-He213 Singh, Mathuresh verfasserin aut Pelletier, Yvan verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in American journal of potato research New York, NY : Springer, 1923 89(2012), 3 vom: 12. Mai, Seite 232-239 (DE-627)54963410X (DE-600)2395546-6 1874-9380 nnns volume:89 year:2012 number:3 day:12 month:05 pages:232-239 https://dx.doi.org/10.1007/s12230-012-9247-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 89 2012 3 12 05 232-239 |
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10.1007/s12230-012-9247-2 doi (DE-627)SPR025506218 (SPR)s12230-012-9247-2-e DE-627 ger DE-627 rakwb eng 630 640 ASE Nanayakkara, Upeksha N. verfasserin aut Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. Potato (dpeaa)DE-He213 Strain (dpeaa)DE-He213 Population distribution (dpeaa)DE-He213 Singh, Mathuresh verfasserin aut Pelletier, Yvan verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in American journal of potato research New York, NY : Springer, 1923 89(2012), 3 vom: 12. Mai, Seite 232-239 (DE-627)54963410X (DE-600)2395546-6 1874-9380 nnns volume:89 year:2012 number:3 day:12 month:05 pages:232-239 https://dx.doi.org/10.1007/s12230-012-9247-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 89 2012 3 12 05 232-239 |
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10.1007/s12230-012-9247-2 doi (DE-627)SPR025506218 (SPR)s12230-012-9247-2-e DE-627 ger DE-627 rakwb eng 630 640 ASE Nanayakkara, Upeksha N. verfasserin aut Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. Potato (dpeaa)DE-He213 Strain (dpeaa)DE-He213 Population distribution (dpeaa)DE-He213 Singh, Mathuresh verfasserin aut Pelletier, Yvan verfasserin aut Nie, Xianzhou verfasserin aut Enthalten in American journal of potato research New York, NY : Springer, 1923 89(2012), 3 vom: 12. Mai, Seite 232-239 (DE-627)54963410X (DE-600)2395546-6 1874-9380 nnns volume:89 year:2012 number:3 day:12 month:05 pages:232-239 https://dx.doi.org/10.1007/s12230-012-9247-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 89 2012 3 12 05 232-239 |
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English |
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Enthalten in American journal of potato research 89(2012), 3 vom: 12. Mai, Seite 232-239 volume:89 year:2012 number:3 day:12 month:05 pages:232-239 |
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Nanayakkara, Upeksha N. @@aut@@ Singh, Mathuresh @@aut@@ Pelletier, Yvan @@aut@@ Nie, Xianzhou @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR025506218</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519230021.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2012 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s12230-012-9247-2</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR025506218</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12230-012-9247-2-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">630</subfield><subfield code="a">640</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Nanayakkara, Upeksha N.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. 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Nanayakkara, Upeksha N. |
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Nanayakkara, Upeksha N. ddc 630 misc Potato misc Strain misc Population distribution Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada |
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630 640 ASE Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada Potato (dpeaa)DE-He213 Strain (dpeaa)DE-He213 Population distribution (dpeaa)DE-He213 |
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Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada |
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Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada |
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Nanayakkara, Upeksha N. |
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American journal of potato research |
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Nanayakkara, Upeksha N. Singh, Mathuresh Pelletier, Yvan Nie, Xianzhou |
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investigation of potato virus y (pvy) strain status and variant population in potatoes in new brunswick, canada |
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Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada |
| abstract |
Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. |
| abstractGer |
Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. |
| abstract_unstemmed |
Abstract The strain diversity and variant population of Potato virus Y (PVY) was investigated in potato lots with high PVY incidence in 2009 in New Brunswick, Canada, using reverse transcription (RT)-PCR, enzyme-linked immunosorbent assay (ELISA) and biological assays. Twenty lots with PVY infection rates >3 % were chosen following post-harvest testing for further investigation. Assays using multiplex RT-PCR revealed the existence of the common ($ PVY^{O} $), the recombinant N:O ($ PVY^{N:O} $) and the recombinant (European) potato tuber necrosis (Eu-$ PVY^{NTN} $) strains. $ PVY^{O} $ was the predominant strain while $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $ were widespread in these potato lots. $ PVY^{N:O} $ and Eu-$ PVY^{NTN} $, either present alone or mixed with $ PVY^{O} $, were found in 19 and 13 of the 20 lots, respectively. Neither the tobacco veinal necrosis strain ($ PVY^{N} $) nor the non-recombinant (North American) $ PVY^{NTN} $ was detected in the potatoes. Among the $ PVY^{O} $ strain group, $ PVY^{O} $-Oz/-FL variant type was the predominant followed by the uncharacterized $ PVY^{O} $ and the $ PVY^{O} $-139/-RB types. Biological assays of representative PVY positive-samples using tobacco and Yukon Gold were consistent with the findings of RT-PCR and serological assays. |
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| title_short |
Investigation of Potato virus Y (PVY) Strain Status and Variant Population in Potatoes in New Brunswick, Canada |
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https://dx.doi.org/10.1007/s12230-012-9247-2 |
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Singh, Mathuresh Pelletier, Yvan Nie, Xianzhou |
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10.1007/s12230-012-9247-2 |
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2024-07-03T16:20:58.639Z |
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| score |
7.4006453 |