A DNA tracer used in column tests for hydrogeology applications
Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Aquilanti, Lucia [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag Berlin Heidelberg 2013 |
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| Übergeordnetes Werk: |
Enthalten in: Environmental earth sciences - Berlin : Springer, 2009, 70(2013), 7 vom: 17. März, Seite 3143-3154 |
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| Übergeordnetes Werk: |
volume:70 ; year:2013 ; number:7 ; day:17 ; month:03 ; pages:3143-3154 |
| Links: |
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| DOI / URN: |
10.1007/s12665-013-2379-y |
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SPR026690063 |
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| 245 | 1 | 2 | |a A DNA tracer used in column tests for hydrogeology applications |
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| 520 | |a Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. | ||
| 650 | 4 | |a Column test |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Hydrogeology tracer |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Quantitative real-time PCR |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Synthetic DNA tracer |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Pore water velocity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Karst hydrogeology |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Clementi, Francesca |4 aut | |
| 700 | 1 | |a Landolfo, Sara |4 aut | |
| 700 | 1 | |a Nanni, Torquato |4 aut | |
| 700 | 1 | |a Palpacelli, Stefano |4 aut | |
| 700 | 1 | |a Tazioli, Alberto |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Environmental earth sciences |d Berlin : Springer, 2009 |g 70(2013), 7 vom: 17. März, Seite 3143-3154 |w (DE-627)599673451 |w (DE-600)2493699-6 |x 1866-6299 |7 nnns |
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10.1007/s12665-013-2379-y doi (DE-627)SPR026690063 (SPR)s12665-013-2379-y-e DE-627 ger DE-627 rakwb eng Aquilanti, Lucia verfasserin aut A DNA tracer used in column tests for hydrogeology applications 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. Column test (dpeaa)DE-He213 Hydrogeology tracer (dpeaa)DE-He213 Quantitative real-time PCR (dpeaa)DE-He213 Synthetic DNA tracer (dpeaa)DE-He213 Pore water velocity (dpeaa)DE-He213 Karst hydrogeology (dpeaa)DE-He213 Clementi, Francesca aut Landolfo, Sara aut Nanni, Torquato aut Palpacelli, Stefano aut Tazioli, Alberto aut Enthalten in Environmental earth sciences Berlin : Springer, 2009 70(2013), 7 vom: 17. März, Seite 3143-3154 (DE-627)599673451 (DE-600)2493699-6 1866-6299 nnns volume:70 year:2013 number:7 day:17 month:03 pages:3143-3154 https://dx.doi.org/10.1007/s12665-013-2379-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2013 7 17 03 3143-3154 |
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10.1007/s12665-013-2379-y doi (DE-627)SPR026690063 (SPR)s12665-013-2379-y-e DE-627 ger DE-627 rakwb eng Aquilanti, Lucia verfasserin aut A DNA tracer used in column tests for hydrogeology applications 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. Column test (dpeaa)DE-He213 Hydrogeology tracer (dpeaa)DE-He213 Quantitative real-time PCR (dpeaa)DE-He213 Synthetic DNA tracer (dpeaa)DE-He213 Pore water velocity (dpeaa)DE-He213 Karst hydrogeology (dpeaa)DE-He213 Clementi, Francesca aut Landolfo, Sara aut Nanni, Torquato aut Palpacelli, Stefano aut Tazioli, Alberto aut Enthalten in Environmental earth sciences Berlin : Springer, 2009 70(2013), 7 vom: 17. März, Seite 3143-3154 (DE-627)599673451 (DE-600)2493699-6 1866-6299 nnns volume:70 year:2013 number:7 day:17 month:03 pages:3143-3154 https://dx.doi.org/10.1007/s12665-013-2379-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2013 7 17 03 3143-3154 |
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10.1007/s12665-013-2379-y doi (DE-627)SPR026690063 (SPR)s12665-013-2379-y-e DE-627 ger DE-627 rakwb eng Aquilanti, Lucia verfasserin aut A DNA tracer used in column tests for hydrogeology applications 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. Column test (dpeaa)DE-He213 Hydrogeology tracer (dpeaa)DE-He213 Quantitative real-time PCR (dpeaa)DE-He213 Synthetic DNA tracer (dpeaa)DE-He213 Pore water velocity (dpeaa)DE-He213 Karst hydrogeology (dpeaa)DE-He213 Clementi, Francesca aut Landolfo, Sara aut Nanni, Torquato aut Palpacelli, Stefano aut Tazioli, Alberto aut Enthalten in Environmental earth sciences Berlin : Springer, 2009 70(2013), 7 vom: 17. März, Seite 3143-3154 (DE-627)599673451 (DE-600)2493699-6 1866-6299 nnns volume:70 year:2013 number:7 day:17 month:03 pages:3143-3154 https://dx.doi.org/10.1007/s12665-013-2379-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2013 7 17 03 3143-3154 |
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10.1007/s12665-013-2379-y doi (DE-627)SPR026690063 (SPR)s12665-013-2379-y-e DE-627 ger DE-627 rakwb eng Aquilanti, Lucia verfasserin aut A DNA tracer used in column tests for hydrogeology applications 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. Column test (dpeaa)DE-He213 Hydrogeology tracer (dpeaa)DE-He213 Quantitative real-time PCR (dpeaa)DE-He213 Synthetic DNA tracer (dpeaa)DE-He213 Pore water velocity (dpeaa)DE-He213 Karst hydrogeology (dpeaa)DE-He213 Clementi, Francesca aut Landolfo, Sara aut Nanni, Torquato aut Palpacelli, Stefano aut Tazioli, Alberto aut Enthalten in Environmental earth sciences Berlin : Springer, 2009 70(2013), 7 vom: 17. März, Seite 3143-3154 (DE-627)599673451 (DE-600)2493699-6 1866-6299 nnns volume:70 year:2013 number:7 day:17 month:03 pages:3143-3154 https://dx.doi.org/10.1007/s12665-013-2379-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2013 7 17 03 3143-3154 |
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10.1007/s12665-013-2379-y doi (DE-627)SPR026690063 (SPR)s12665-013-2379-y-e DE-627 ger DE-627 rakwb eng Aquilanti, Lucia verfasserin aut A DNA tracer used in column tests for hydrogeology applications 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. Column test (dpeaa)DE-He213 Hydrogeology tracer (dpeaa)DE-He213 Quantitative real-time PCR (dpeaa)DE-He213 Synthetic DNA tracer (dpeaa)DE-He213 Pore water velocity (dpeaa)DE-He213 Karst hydrogeology (dpeaa)DE-He213 Clementi, Francesca aut Landolfo, Sara aut Nanni, Torquato aut Palpacelli, Stefano aut Tazioli, Alberto aut Enthalten in Environmental earth sciences Berlin : Springer, 2009 70(2013), 7 vom: 17. März, Seite 3143-3154 (DE-627)599673451 (DE-600)2493699-6 1866-6299 nnns volume:70 year:2013 number:7 day:17 month:03 pages:3143-3154 https://dx.doi.org/10.1007/s12665-013-2379-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2013 7 17 03 3143-3154 |
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Enthalten in Environmental earth sciences 70(2013), 7 vom: 17. März, Seite 3143-3154 volume:70 year:2013 number:7 day:17 month:03 pages:3143-3154 |
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Column test Hydrogeology tracer Quantitative real-time PCR Synthetic DNA tracer Pore water velocity Karst hydrogeology |
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Environmental earth sciences |
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Aquilanti, Lucia @@aut@@ Clementi, Francesca @@aut@@ Landolfo, Sara @@aut@@ Nanni, Torquato @@aut@@ Palpacelli, Stefano @@aut@@ Tazioli, Alberto @@aut@@ |
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Aquilanti, Lucia |
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Aquilanti, Lucia misc Column test misc Hydrogeology tracer misc Quantitative real-time PCR misc Synthetic DNA tracer misc Pore water velocity misc Karst hydrogeology A DNA tracer used in column tests for hydrogeology applications |
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A DNA tracer used in column tests for hydrogeology applications Column test (dpeaa)DE-He213 Hydrogeology tracer (dpeaa)DE-He213 Quantitative real-time PCR (dpeaa)DE-He213 Synthetic DNA tracer (dpeaa)DE-He213 Pore water velocity (dpeaa)DE-He213 Karst hydrogeology (dpeaa)DE-He213 |
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dna tracer used in column tests for hydrogeology applications |
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A DNA tracer used in column tests for hydrogeology applications |
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Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. © Springer-Verlag Berlin Heidelberg 2013 |
| abstractGer |
Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. © Springer-Verlag Berlin Heidelberg 2013 |
| abstract_unstemmed |
Abstract Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field. © Springer-Verlag Berlin Heidelberg 2013 |
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A DNA tracer used in column tests for hydrogeology applications |
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Clementi, Francesca Landolfo, Sara Nanni, Torquato Palpacelli, Stefano Tazioli, Alberto |
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Clementi, Francesca Landolfo, Sara Nanni, Torquato Palpacelli, Stefano Tazioli, Alberto |
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2024-07-03T22:10:45.011Z |
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| score |
7.397662 |