Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells

Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Strating, Jeroen RPM [verfasserIn] Martens, Gerard JM

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Schlagwörter:	Golgi Apparatus Secretory Pathway Neuroendocrine Cell Hyperactive Cell Neuronal Lobe

Anmerkung:	© Strating and Martens; licensee BioMed Central Ltd. 2009

Übergeordnetes Werk:	Enthalten in: BMC cell biology - London : BioMed Central, 2000, 10(2009), 1 vom: 07. Mai
Übergeordnetes Werk:	volume:10 ; year:2009 ; number:1 ; day:07 ; month:05

Links:	Volltext

DOI / URN:	10.1186/1471-2121-10-35

Katalog-ID:	SPR026934744

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520			\|a Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals.
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matchkey_str	article:14712121:2009----::nopeeotrnltoapoomnmdfctosnyeat
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allfields	10.1186/1471-2121-10-35 doi (DE-627)SPR026934744 (SPR)1471-2121-10-35-e DE-627 ger DE-627 rakwb eng Strating, Jeroen RPM verfasserin aut Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Strating and Martens; licensee BioMed Central Ltd. 2009 Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. Golgi Apparatus (dpeaa)DE-He213 Secretory Pathway (dpeaa)DE-He213 Neuroendocrine Cell (dpeaa)DE-He213 Hyperactive Cell (dpeaa)DE-He213 Neuronal Lobe (dpeaa)DE-He213 Martens, Gerard JM aut Enthalten in BMC cell biology London : BioMed Central, 2000 10(2009), 1 vom: 07. Mai (DE-627)326644830 (DE-600)2041486-9 1471-2121 nnns volume:10 year:2009 number:1 day:07 month:05 https://dx.doi.org/10.1186/1471-2121-10-35 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1 07 05
spelling	10.1186/1471-2121-10-35 doi (DE-627)SPR026934744 (SPR)1471-2121-10-35-e DE-627 ger DE-627 rakwb eng Strating, Jeroen RPM verfasserin aut Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Strating and Martens; licensee BioMed Central Ltd. 2009 Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. Golgi Apparatus (dpeaa)DE-He213 Secretory Pathway (dpeaa)DE-He213 Neuroendocrine Cell (dpeaa)DE-He213 Hyperactive Cell (dpeaa)DE-He213 Neuronal Lobe (dpeaa)DE-He213 Martens, Gerard JM aut Enthalten in BMC cell biology London : BioMed Central, 2000 10(2009), 1 vom: 07. Mai (DE-627)326644830 (DE-600)2041486-9 1471-2121 nnns volume:10 year:2009 number:1 day:07 month:05 https://dx.doi.org/10.1186/1471-2121-10-35 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1 07 05
allfields_unstemmed	10.1186/1471-2121-10-35 doi (DE-627)SPR026934744 (SPR)1471-2121-10-35-e DE-627 ger DE-627 rakwb eng Strating, Jeroen RPM verfasserin aut Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Strating and Martens; licensee BioMed Central Ltd. 2009 Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. Golgi Apparatus (dpeaa)DE-He213 Secretory Pathway (dpeaa)DE-He213 Neuroendocrine Cell (dpeaa)DE-He213 Hyperactive Cell (dpeaa)DE-He213 Neuronal Lobe (dpeaa)DE-He213 Martens, Gerard JM aut Enthalten in BMC cell biology London : BioMed Central, 2000 10(2009), 1 vom: 07. Mai (DE-627)326644830 (DE-600)2041486-9 1471-2121 nnns volume:10 year:2009 number:1 day:07 month:05 https://dx.doi.org/10.1186/1471-2121-10-35 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1 07 05
allfieldsGer	10.1186/1471-2121-10-35 doi (DE-627)SPR026934744 (SPR)1471-2121-10-35-e DE-627 ger DE-627 rakwb eng Strating, Jeroen RPM verfasserin aut Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Strating and Martens; licensee BioMed Central Ltd. 2009 Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. Golgi Apparatus (dpeaa)DE-He213 Secretory Pathway (dpeaa)DE-He213 Neuroendocrine Cell (dpeaa)DE-He213 Hyperactive Cell (dpeaa)DE-He213 Neuronal Lobe (dpeaa)DE-He213 Martens, Gerard JM aut Enthalten in BMC cell biology London : BioMed Central, 2000 10(2009), 1 vom: 07. Mai (DE-627)326644830 (DE-600)2041486-9 1471-2121 nnns volume:10 year:2009 number:1 day:07 month:05 https://dx.doi.org/10.1186/1471-2121-10-35 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1 07 05
allfieldsSound	10.1186/1471-2121-10-35 doi (DE-627)SPR026934744 (SPR)1471-2121-10-35-e DE-627 ger DE-627 rakwb eng Strating, Jeroen RPM verfasserin aut Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Strating and Martens; licensee BioMed Central Ltd. 2009 Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. Golgi Apparatus (dpeaa)DE-He213 Secretory Pathway (dpeaa)DE-He213 Neuroendocrine Cell (dpeaa)DE-He213 Hyperactive Cell (dpeaa)DE-He213 Neuronal Lobe (dpeaa)DE-He213 Martens, Gerard JM aut Enthalten in BMC cell biology London : BioMed Central, 2000 10(2009), 1 vom: 07. Mai (DE-627)326644830 (DE-600)2041486-9 1471-2121 nnns volume:10 year:2009 number:1 day:07 month:05 https://dx.doi.org/10.1186/1471-2121-10-35 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1 07 05
language	English
source	Enthalten in BMC cell biology 10(2009), 1 vom: 07. Mai volume:10 year:2009 number:1 day:07 month:05
sourceStr	Enthalten in BMC cell biology 10(2009), 1 vom: 07. Mai volume:10 year:2009 number:1 day:07 month:05
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topic_facet	Golgi Apparatus Secretory Pathway Neuroendocrine Cell Hyperactive Cell Neuronal Lobe
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author	Strating, Jeroen RPM
spellingShingle	Strating, Jeroen RPM misc Golgi Apparatus misc Secretory Pathway misc Neuroendocrine Cell misc Hyperactive Cell misc Neuronal Lobe Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
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topic_title	Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells Golgi Apparatus (dpeaa)DE-He213 Secretory Pathway (dpeaa)DE-He213 Neuroendocrine Cell (dpeaa)DE-He213 Hyperactive Cell (dpeaa)DE-He213 Neuronal Lobe (dpeaa)DE-He213
topic	misc Golgi Apparatus misc Secretory Pathway misc Neuroendocrine Cell misc Hyperactive Cell misc Neuronal Lobe
topic_unstemmed	misc Golgi Apparatus misc Secretory Pathway misc Neuroendocrine Cell misc Hyperactive Cell misc Neuronal Lobe
topic_browse	misc Golgi Apparatus misc Secretory Pathway misc Neuroendocrine Cell misc Hyperactive Cell misc Neuronal Lobe
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title	Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
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title_full	Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
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title_sort	incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
title_auth	Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
abstract	Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. © Strating and Martens; licensee BioMed Central Ltd. 2009
abstractGer	Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. © Strating and Martens; licensee BioMed Central Ltd. 2009
abstract_unstemmed	Background In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals. © Strating and Martens; licensee BioMed Central Ltd. 2009
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title_short	Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
url	https://dx.doi.org/10.1186/1471-2121-10-35
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Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway. Results We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased. Conclusion Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. 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Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells

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