Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing

Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiolog...
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Autor*in:	Dussmann, Philipp [verfasserIn] Pagel, Judith I Vogel, Sabina Magnusson, Terese Zimmermann, Rene Wagner, Ernst Schaper, Wolfgang Ogris, Manfred Deindl, Elisabeth

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	Vascular Endothelial Growth Factor Luciferase Activity Liver Regeneration Cell Vascular Endothelial Growth Factor Vascular Endothelial Growth Factor Promoter

Anmerkung:	© Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC developmental biology - London : BioMed Central, 2001, 11(2011), 1 vom: 20. Mai
Übergeordnetes Werk:	volume:11 ; year:2011 ; number:1 ; day:20 ; month:05

Links:	Volltext

DOI / URN:	10.1186/1471-213X-11-28

Katalog-ID:	SPR026947978

Internformat


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520			\|a Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo.
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allfields	10.1186/1471-213X-11-28 doi (DE-627)SPR026947978 (SPR)1471-213X-11-28-e DE-627 ger DE-627 rakwb eng Dussmann, Philipp verfasserin aut Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. Vascular Endothelial Growth Factor (dpeaa)DE-He213 Luciferase Activity (dpeaa)DE-He213 Liver Regeneration (dpeaa)DE-He213 Cell Vascular Endothelial Growth Factor (dpeaa)DE-He213 Vascular Endothelial Growth Factor Promoter (dpeaa)DE-He213 Pagel, Judith I aut Vogel, Sabina aut Magnusson, Terese aut Zimmermann, Rene aut Wagner, Ernst aut Schaper, Wolfgang aut Ogris, Manfred aut Deindl, Elisabeth aut Enthalten in BMC developmental biology London : BioMed Central, 2001 11(2011), 1 vom: 20. Mai (DE-627)326644881 (DE-600)2041492-4 1471-213X nnns volume:11 year:2011 number:1 day:20 month:05 https://dx.doi.org/10.1186/1471-213X-11-28 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1 20 05
spelling	10.1186/1471-213X-11-28 doi (DE-627)SPR026947978 (SPR)1471-213X-11-28-e DE-627 ger DE-627 rakwb eng Dussmann, Philipp verfasserin aut Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. Vascular Endothelial Growth Factor (dpeaa)DE-He213 Luciferase Activity (dpeaa)DE-He213 Liver Regeneration (dpeaa)DE-He213 Cell Vascular Endothelial Growth Factor (dpeaa)DE-He213 Vascular Endothelial Growth Factor Promoter (dpeaa)DE-He213 Pagel, Judith I aut Vogel, Sabina aut Magnusson, Terese aut Zimmermann, Rene aut Wagner, Ernst aut Schaper, Wolfgang aut Ogris, Manfred aut Deindl, Elisabeth aut Enthalten in BMC developmental biology London : BioMed Central, 2001 11(2011), 1 vom: 20. Mai (DE-627)326644881 (DE-600)2041492-4 1471-213X nnns volume:11 year:2011 number:1 day:20 month:05 https://dx.doi.org/10.1186/1471-213X-11-28 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1 20 05
allfields_unstemmed	10.1186/1471-213X-11-28 doi (DE-627)SPR026947978 (SPR)1471-213X-11-28-e DE-627 ger DE-627 rakwb eng Dussmann, Philipp verfasserin aut Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. Vascular Endothelial Growth Factor (dpeaa)DE-He213 Luciferase Activity (dpeaa)DE-He213 Liver Regeneration (dpeaa)DE-He213 Cell Vascular Endothelial Growth Factor (dpeaa)DE-He213 Vascular Endothelial Growth Factor Promoter (dpeaa)DE-He213 Pagel, Judith I aut Vogel, Sabina aut Magnusson, Terese aut Zimmermann, Rene aut Wagner, Ernst aut Schaper, Wolfgang aut Ogris, Manfred aut Deindl, Elisabeth aut Enthalten in BMC developmental biology London : BioMed Central, 2001 11(2011), 1 vom: 20. Mai (DE-627)326644881 (DE-600)2041492-4 1471-213X nnns volume:11 year:2011 number:1 day:20 month:05 https://dx.doi.org/10.1186/1471-213X-11-28 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1 20 05
allfieldsGer	10.1186/1471-213X-11-28 doi (DE-627)SPR026947978 (SPR)1471-213X-11-28-e DE-627 ger DE-627 rakwb eng Dussmann, Philipp verfasserin aut Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. Vascular Endothelial Growth Factor (dpeaa)DE-He213 Luciferase Activity (dpeaa)DE-He213 Liver Regeneration (dpeaa)DE-He213 Cell Vascular Endothelial Growth Factor (dpeaa)DE-He213 Vascular Endothelial Growth Factor Promoter (dpeaa)DE-He213 Pagel, Judith I aut Vogel, Sabina aut Magnusson, Terese aut Zimmermann, Rene aut Wagner, Ernst aut Schaper, Wolfgang aut Ogris, Manfred aut Deindl, Elisabeth aut Enthalten in BMC developmental biology London : BioMed Central, 2001 11(2011), 1 vom: 20. Mai (DE-627)326644881 (DE-600)2041492-4 1471-213X nnns volume:11 year:2011 number:1 day:20 month:05 https://dx.doi.org/10.1186/1471-213X-11-28 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1 20 05
allfieldsSound	10.1186/1471-213X-11-28 doi (DE-627)SPR026947978 (SPR)1471-213X-11-28-e DE-627 ger DE-627 rakwb eng Dussmann, Philipp verfasserin aut Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. Vascular Endothelial Growth Factor (dpeaa)DE-He213 Luciferase Activity (dpeaa)DE-He213 Liver Regeneration (dpeaa)DE-He213 Cell Vascular Endothelial Growth Factor (dpeaa)DE-He213 Vascular Endothelial Growth Factor Promoter (dpeaa)DE-He213 Pagel, Judith I aut Vogel, Sabina aut Magnusson, Terese aut Zimmermann, Rene aut Wagner, Ernst aut Schaper, Wolfgang aut Ogris, Manfred aut Deindl, Elisabeth aut Enthalten in BMC developmental biology London : BioMed Central, 2001 11(2011), 1 vom: 20. Mai (DE-627)326644881 (DE-600)2041492-4 1471-213X nnns volume:11 year:2011 number:1 day:20 month:05 https://dx.doi.org/10.1186/1471-213X-11-28 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1 20 05
language	English
source	Enthalten in BMC developmental biology 11(2011), 1 vom: 20. Mai volume:11 year:2011 number:1 day:20 month:05
sourceStr	Enthalten in BMC developmental biology 11(2011), 1 vom: 20. Mai volume:11 year:2011 number:1 day:20 month:05
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Vascular Endothelial Growth Factor Luciferase Activity Liver Regeneration Cell Vascular Endothelial Growth Factor Vascular Endothelial Growth Factor Promoter
isfreeaccess_bool	true
container_title	BMC developmental biology
authorswithroles_txt_mv	Dussmann, Philipp @@aut@@ Pagel, Judith I @@aut@@ Vogel, Sabina @@aut@@ Magnusson, Terese @@aut@@ Zimmermann, Rene @@aut@@ Wagner, Ernst @@aut@@ Schaper, Wolfgang @@aut@@ Ogris, Manfred @@aut@@ Deindl, Elisabeth @@aut@@
publishDateDaySort_date	2011-05-20T00:00:00Z
hierarchy_top_id	326644881
id	SPR026947978
language_de	englisch
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author	Dussmann, Philipp
spellingShingle	Dussmann, Philipp misc Vascular Endothelial Growth Factor misc Luciferase Activity misc Liver Regeneration misc Cell Vascular Endothelial Growth Factor misc Vascular Endothelial Growth Factor Promoter Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing
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topic_title	Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing Vascular Endothelial Growth Factor (dpeaa)DE-He213 Luciferase Activity (dpeaa)DE-He213 Liver Regeneration (dpeaa)DE-He213 Cell Vascular Endothelial Growth Factor (dpeaa)DE-He213 Vascular Endothelial Growth Factor Promoter (dpeaa)DE-He213
topic	misc Vascular Endothelial Growth Factor misc Luciferase Activity misc Liver Regeneration misc Cell Vascular Endothelial Growth Factor misc Vascular Endothelial Growth Factor Promoter
topic_unstemmed	misc Vascular Endothelial Growth Factor misc Luciferase Activity misc Liver Regeneration misc Cell Vascular Endothelial Growth Factor misc Vascular Endothelial Growth Factor Promoter
topic_browse	misc Vascular Endothelial Growth Factor misc Luciferase Activity misc Liver Regeneration misc Cell Vascular Endothelial Growth Factor misc Vascular Endothelial Growth Factor Promoter
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title	Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing
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title_full	Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing
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author_browse	Dussmann, Philipp Pagel, Judith I Vogel, Sabina Magnusson, Terese Zimmermann, Rene Wagner, Ernst Schaper, Wolfgang Ogris, Manfred Deindl, Elisabeth
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title_sort	live in vivo imaging of egr-1 promoter activity during neonatal development, liver regeneration and wound healing
title_auth	Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing
abstract	Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Background The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing, we demonstrated that Egr-1 promoter activity was upregulated at the site of injury. Conclusion Taken together, we have developed a transgenic mouse model that allows real time in vivo imaging of the Egr-1 promoter activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. © Dussmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing
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Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing

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