Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Hou, Jinxing [verfasserIn] An, Xiaopeng Song, Yuxuan Cao, Binyun Yang, Heping Zhang, Zhou Shen, Wenzheng Li, Yunpu

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	Mammary gland Dairy goats Colostrum Solexa sequencing

Anmerkung:	© The Author(s). 2017

Übergeordnetes Werk:	Enthalten in: BMC genetics - London : BioMed Central, 2000, 18(2017), 1 vom: 02. Mai
Übergeordnetes Werk:	volume:18 ; year:2017 ; number:1 ; day:02 ; month:05

Links:	Volltext

DOI / URN:	10.1186/s12863-017-0498-2

Katalog-ID:	SPR027016560

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245	1	0	\|a Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages
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520			\|a Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland.
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matchkey_str	article:14712156:2017----::eetoadoprsnfirraitearnmmaylntsusfo
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publishDate	2017
allfields	10.1186/s12863-017-0498-2 doi (DE-627)SPR027016560 (SPR)s12863-017-0498-2-e DE-627 ger DE-627 rakwb eng Hou, Jinxing verfasserin aut Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. Mammary gland (dpeaa)DE-He213 Dairy goats (dpeaa)DE-He213 Colostrum (dpeaa)DE-He213 Solexa sequencing (dpeaa)DE-He213 An, Xiaopeng aut Song, Yuxuan aut Cao, Binyun aut Yang, Heping aut Zhang, Zhou aut Shen, Wenzheng aut Li, Yunpu aut Enthalten in BMC genetics London : BioMed Central, 2000 18(2017), 1 vom: 02. Mai (DE-627)326644938 (DE-600)2041497-3 1471-2156 nnns volume:18 year:2017 number:1 day:02 month:05 https://dx.doi.org/10.1186/s12863-017-0498-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2017 1 02 05
spelling	10.1186/s12863-017-0498-2 doi (DE-627)SPR027016560 (SPR)s12863-017-0498-2-e DE-627 ger DE-627 rakwb eng Hou, Jinxing verfasserin aut Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. Mammary gland (dpeaa)DE-He213 Dairy goats (dpeaa)DE-He213 Colostrum (dpeaa)DE-He213 Solexa sequencing (dpeaa)DE-He213 An, Xiaopeng aut Song, Yuxuan aut Cao, Binyun aut Yang, Heping aut Zhang, Zhou aut Shen, Wenzheng aut Li, Yunpu aut Enthalten in BMC genetics London : BioMed Central, 2000 18(2017), 1 vom: 02. Mai (DE-627)326644938 (DE-600)2041497-3 1471-2156 nnns volume:18 year:2017 number:1 day:02 month:05 https://dx.doi.org/10.1186/s12863-017-0498-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2017 1 02 05
allfields_unstemmed	10.1186/s12863-017-0498-2 doi (DE-627)SPR027016560 (SPR)s12863-017-0498-2-e DE-627 ger DE-627 rakwb eng Hou, Jinxing verfasserin aut Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. Mammary gland (dpeaa)DE-He213 Dairy goats (dpeaa)DE-He213 Colostrum (dpeaa)DE-He213 Solexa sequencing (dpeaa)DE-He213 An, Xiaopeng aut Song, Yuxuan aut Cao, Binyun aut Yang, Heping aut Zhang, Zhou aut Shen, Wenzheng aut Li, Yunpu aut Enthalten in BMC genetics London : BioMed Central, 2000 18(2017), 1 vom: 02. Mai (DE-627)326644938 (DE-600)2041497-3 1471-2156 nnns volume:18 year:2017 number:1 day:02 month:05 https://dx.doi.org/10.1186/s12863-017-0498-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2017 1 02 05
allfieldsGer	10.1186/s12863-017-0498-2 doi (DE-627)SPR027016560 (SPR)s12863-017-0498-2-e DE-627 ger DE-627 rakwb eng Hou, Jinxing verfasserin aut Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. Mammary gland (dpeaa)DE-He213 Dairy goats (dpeaa)DE-He213 Colostrum (dpeaa)DE-He213 Solexa sequencing (dpeaa)DE-He213 An, Xiaopeng aut Song, Yuxuan aut Cao, Binyun aut Yang, Heping aut Zhang, Zhou aut Shen, Wenzheng aut Li, Yunpu aut Enthalten in BMC genetics London : BioMed Central, 2000 18(2017), 1 vom: 02. Mai (DE-627)326644938 (DE-600)2041497-3 1471-2156 nnns volume:18 year:2017 number:1 day:02 month:05 https://dx.doi.org/10.1186/s12863-017-0498-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2017 1 02 05
allfieldsSound	10.1186/s12863-017-0498-2 doi (DE-627)SPR027016560 (SPR)s12863-017-0498-2-e DE-627 ger DE-627 rakwb eng Hou, Jinxing verfasserin aut Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. Mammary gland (dpeaa)DE-He213 Dairy goats (dpeaa)DE-He213 Colostrum (dpeaa)DE-He213 Solexa sequencing (dpeaa)DE-He213 An, Xiaopeng aut Song, Yuxuan aut Cao, Binyun aut Yang, Heping aut Zhang, Zhou aut Shen, Wenzheng aut Li, Yunpu aut Enthalten in BMC genetics London : BioMed Central, 2000 18(2017), 1 vom: 02. Mai (DE-627)326644938 (DE-600)2041497-3 1471-2156 nnns volume:18 year:2017 number:1 day:02 month:05 https://dx.doi.org/10.1186/s12863-017-0498-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2017 1 02 05
language	English
source	Enthalten in BMC genetics 18(2017), 1 vom: 02. Mai volume:18 year:2017 number:1 day:02 month:05
sourceStr	Enthalten in BMC genetics 18(2017), 1 vom: 02. Mai volume:18 year:2017 number:1 day:02 month:05
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Mammary gland Dairy goats Colostrum Solexa sequencing
isfreeaccess_bool	true
container_title	BMC genetics
authorswithroles_txt_mv	Hou, Jinxing @@aut@@ An, Xiaopeng @@aut@@ Song, Yuxuan @@aut@@ Cao, Binyun @@aut@@ Yang, Heping @@aut@@ Zhang, Zhou @@aut@@ Shen, Wenzheng @@aut@@ Li, Yunpu @@aut@@
publishDateDaySort_date	2017-05-02T00:00:00Z
hierarchy_top_id	326644938
id	SPR027016560
language_de	englisch
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A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. 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author	Hou, Jinxing
spellingShingle	Hou, Jinxing misc Mammary gland misc Dairy goats misc Colostrum misc Solexa sequencing Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages
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topic_title	Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages Mammary gland (dpeaa)DE-He213 Dairy goats (dpeaa)DE-He213 Colostrum (dpeaa)DE-He213 Solexa sequencing (dpeaa)DE-He213
topic	misc Mammary gland misc Dairy goats misc Colostrum misc Solexa sequencing
topic_unstemmed	misc Mammary gland misc Dairy goats misc Colostrum misc Solexa sequencing
topic_browse	misc Mammary gland misc Dairy goats misc Colostrum misc Solexa sequencing
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages
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title_full	Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages
author_sort	Hou, Jinxing
journal	BMC genetics
journalStr	BMC genetics
lang_code	eng
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author_browse	Hou, Jinxing An, Xiaopeng Song, Yuxuan Cao, Binyun Yang, Heping Zhang, Zhou Shen, Wenzheng Li, Yunpu
container_volume	18
format_se	Elektronische Aufsätze
author-letter	Hou, Jinxing
doi_str_mv	10.1186/s12863-017-0498-2
title_sort	detection and comparison of micrornas in the caprine mammary gland tissues of colostrum and common milk stages
title_auth	Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages
abstract	Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. © The Author(s). 2017
abstractGer	Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. © The Author(s). 2017
abstract_unstemmed	Background MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. Result This study identified 131 differentially expressed miRNAs (P < 0.05 and $ log_{2} $ colostrum normalized expression (NE)/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and $ log_{2} $ colostrum NE/peak lactation NE > 1 or $ log_{2} $ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland. © The Author(s). 2017
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title_short	Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages
url	https://dx.doi.org/10.1186/s12863-017-0498-2
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author2	An, Xiaopeng Song, Yuxuan Cao, Binyun Yang, Heping Zhang, Zhou Shen, Wenzheng Li, Yunpu
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up_date	2024-07-03T23:57:04.567Z
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The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. Conclusions Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. 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Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

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