Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha

Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to huma...
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Autor*in:	Chen, Chuan-Hung [verfasserIn] Kuo, Tony Chien-Yen Yang, Meng-Han Chien, Ting-Ying Chu, Mei-Ju Huang, Li-Chun Chen, Chien-Yu Lo, Hsiao-Feng Jeng, Shih-Tong Chen, Long-Fang O

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Agarwood Cucurbitacin Aquilaria Genome

Anmerkung:	© Chen et al.; licensee BioMed Central Ltd. 2014

Übergeordnetes Werk:	Enthalten in: BMC genomics - London : BioMed Central, 2000, 15(2014), 1 vom: 09. Juli
Übergeordnetes Werk:	volume:15 ; year:2014 ; number:1 ; day:09 ; month:07

Links:	Volltext

DOI / URN:	10.1186/1471-2164-15-578

Katalog-ID:	SPR027092348

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520			\|a Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants.
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author_variant	c h c chc t c y k tcy tcyk m h y mhy t y c tyc m j c mjc l c h lch c y c cyc h f l hfl s t j stj l f o c lfo lfoc
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allfields	10.1186/1471-2164-15-578 doi (DE-627)SPR027092348 (SPR)1471-2164-15-578-e DE-627 ger DE-627 rakwb eng Chen, Chuan-Hung verfasserin aut Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Chen et al.; licensee BioMed Central Ltd. 2014 Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. Agarwood (dpeaa)DE-He213 Cucurbitacin (dpeaa)DE-He213 Aquilaria (dpeaa)DE-He213 Genome (dpeaa)DE-He213 Kuo, Tony Chien-Yen aut Yang, Meng-Han aut Chien, Ting-Ying aut Chu, Mei-Ju aut Huang, Li-Chun aut Chen, Chien-Yu aut Lo, Hsiao-Feng aut Jeng, Shih-Tong aut Chen, Long-Fang O aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 09. Juli (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:09 month:07 https://dx.doi.org/10.1186/1471-2164-15-578 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 09 07
spelling	10.1186/1471-2164-15-578 doi (DE-627)SPR027092348 (SPR)1471-2164-15-578-e DE-627 ger DE-627 rakwb eng Chen, Chuan-Hung verfasserin aut Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Chen et al.; licensee BioMed Central Ltd. 2014 Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. Agarwood (dpeaa)DE-He213 Cucurbitacin (dpeaa)DE-He213 Aquilaria (dpeaa)DE-He213 Genome (dpeaa)DE-He213 Kuo, Tony Chien-Yen aut Yang, Meng-Han aut Chien, Ting-Ying aut Chu, Mei-Ju aut Huang, Li-Chun aut Chen, Chien-Yu aut Lo, Hsiao-Feng aut Jeng, Shih-Tong aut Chen, Long-Fang O aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 09. Juli (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:09 month:07 https://dx.doi.org/10.1186/1471-2164-15-578 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 09 07
allfields_unstemmed	10.1186/1471-2164-15-578 doi (DE-627)SPR027092348 (SPR)1471-2164-15-578-e DE-627 ger DE-627 rakwb eng Chen, Chuan-Hung verfasserin aut Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Chen et al.; licensee BioMed Central Ltd. 2014 Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. Agarwood (dpeaa)DE-He213 Cucurbitacin (dpeaa)DE-He213 Aquilaria (dpeaa)DE-He213 Genome (dpeaa)DE-He213 Kuo, Tony Chien-Yen aut Yang, Meng-Han aut Chien, Ting-Ying aut Chu, Mei-Ju aut Huang, Li-Chun aut Chen, Chien-Yu aut Lo, Hsiao-Feng aut Jeng, Shih-Tong aut Chen, Long-Fang O aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 09. Juli (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:09 month:07 https://dx.doi.org/10.1186/1471-2164-15-578 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 09 07
allfieldsGer	10.1186/1471-2164-15-578 doi (DE-627)SPR027092348 (SPR)1471-2164-15-578-e DE-627 ger DE-627 rakwb eng Chen, Chuan-Hung verfasserin aut Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Chen et al.; licensee BioMed Central Ltd. 2014 Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. Agarwood (dpeaa)DE-He213 Cucurbitacin (dpeaa)DE-He213 Aquilaria (dpeaa)DE-He213 Genome (dpeaa)DE-He213 Kuo, Tony Chien-Yen aut Yang, Meng-Han aut Chien, Ting-Ying aut Chu, Mei-Ju aut Huang, Li-Chun aut Chen, Chien-Yu aut Lo, Hsiao-Feng aut Jeng, Shih-Tong aut Chen, Long-Fang O aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 09. Juli (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:09 month:07 https://dx.doi.org/10.1186/1471-2164-15-578 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 09 07
allfieldsSound	10.1186/1471-2164-15-578 doi (DE-627)SPR027092348 (SPR)1471-2164-15-578-e DE-627 ger DE-627 rakwb eng Chen, Chuan-Hung verfasserin aut Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Chen et al.; licensee BioMed Central Ltd. 2014 Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. Agarwood (dpeaa)DE-He213 Cucurbitacin (dpeaa)DE-He213 Aquilaria (dpeaa)DE-He213 Genome (dpeaa)DE-He213 Kuo, Tony Chien-Yen aut Yang, Meng-Han aut Chien, Ting-Ying aut Chu, Mei-Ju aut Huang, Li-Chun aut Chen, Chien-Yu aut Lo, Hsiao-Feng aut Jeng, Shih-Tong aut Chen, Long-Fang O aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 09. Juli (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:09 month:07 https://dx.doi.org/10.1186/1471-2164-15-578 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 09 07
language	English
source	Enthalten in BMC genomics 15(2014), 1 vom: 09. Juli volume:15 year:2014 number:1 day:09 month:07
sourceStr	Enthalten in BMC genomics 15(2014), 1 vom: 09. Juli volume:15 year:2014 number:1 day:09 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Agarwood Cucurbitacin Aquilaria Genome
isfreeaccess_bool	true
container_title	BMC genomics
authorswithroles_txt_mv	Chen, Chuan-Hung @@aut@@ Kuo, Tony Chien-Yen @@aut@@ Yang, Meng-Han @@aut@@ Chien, Ting-Ying @@aut@@ Chu, Mei-Ju @@aut@@ Huang, Li-Chun @@aut@@ Chen, Chien-Yu @@aut@@ Lo, Hsiao-Feng @@aut@@ Jeng, Shih-Tong @@aut@@ Chen, Long-Fang O @@aut@@
publishDateDaySort_date	2014-07-09T00:00:00Z
hierarchy_top_id	326644954
id	SPR027092348
language_de	englisch
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Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. 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author	Chen, Chuan-Hung
spellingShingle	Chen, Chuan-Hung misc Agarwood misc Cucurbitacin misc Aquilaria misc Genome Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha
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topic_title	Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha Agarwood (dpeaa)DE-He213 Cucurbitacin (dpeaa)DE-He213 Aquilaria (dpeaa)DE-He213 Genome (dpeaa)DE-He213
topic	misc Agarwood misc Cucurbitacin misc Aquilaria misc Genome
topic_unstemmed	misc Agarwood misc Cucurbitacin misc Aquilaria misc Genome
topic_browse	misc Agarwood misc Cucurbitacin misc Aquilaria misc Genome
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title	Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha
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title_full	Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha
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author_browse	Chen, Chuan-Hung Kuo, Tony Chien-Yen Yang, Meng-Han Chien, Ting-Ying Chu, Mei-Ju Huang, Li-Chun Chen, Chien-Yu Lo, Hsiao-Feng Jeng, Shih-Tong Chen, Long-Fang O
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author-letter	Chen, Chuan-Hung
doi_str_mv	10.1186/1471-2164-15-578
title_sort	identification of cucurbitacins and assembly of a draft genome for aquilaria agallocha
title_auth	Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha
abstract	Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. © Chen et al.; licensee BioMed Central Ltd. 2014
abstractGer	Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. © Chen et al.; licensee BioMed Central Ltd. 2014
abstract_unstemmed	Background Agarwood is derived from Aquilaria trees, the trade of which has come under strict control with a listing in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Many secondary metabolites of agarwood are known to have medicinal value to humans, including compounds that have been shown to elicit sedative effects and exhibit anti-cancer properties. However, little is known about the genome, transcriptome, and the biosynthetic pathways responsible for producing such secondary metabolites in agarwood. Results In this study, we present a draft genome and a putative pathway for cucurbitacin E and I, compounds with known medicinal value, from in vitro Aquilaria agallocha agarwood. DNA and RNA data are utilized to annotate many genes and protein functions in the draft genome. The expression changes for cucurbitacin E and I are shown to be consistent with known responses of A. agallocha to biotic stress and a set of homologous genes in Arabidopsis thaliana related to cucurbitacin bio-synthesis is presented and validated through qRT-PCR. Conclusions This study is the first attempt to identify cucurbitacin E and I from in vitro agarwood and the first draft genome for any species of Aquilaria. The results of this study will aid in future investigations of secondary metabolite pathways in Aquilaria and other non-model medicinal plants. © Chen et al.; licensee BioMed Central Ltd. 2014
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title_short	Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha
url	https://dx.doi.org/10.1186/1471-2164-15-578
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author2	Kuo, Tony Chien-Yen Yang, Meng-Han Chien, Ting-Ying Chu, Mei-Ju Huang, Li-Chun Chen, Chien-Yu Lo, Hsiao-Feng Jeng, Shih-Tong Chen, Long-Fang O
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Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha

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