An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines

Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carci...
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Autor*in:	Vencken, Sebastian F [verfasserIn] Sethupathy, Praveen Blackshields, Gordon Spillane, Cathy Elbaruni, Salah Sheils, Orla Gallagher, Michael F O’Leary, John J

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	SOX2 microRNA Embryonic stem cell Embryonal carcinoma Pluripotency EMT

Anmerkung:	© Vencken et al.; licensee BioMed Central Ltd. 2014

Übergeordnetes Werk:	Enthalten in: BMC genomics - London : BioMed Central, 2000, 15(2014), 1 vom: 25. Aug.
Übergeordnetes Werk:	volume:15 ; year:2014 ; number:1 ; day:25 ; month:08

Links:	Volltext

DOI / URN:	10.1186/1471-2164-15-711

Katalog-ID:	SPR027094103

Internformat


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245	1	3	\|a An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines
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520			\|a Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT.
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700	1		\|a Sethupathy, Praveen \|4 aut
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700	1		\|a Elbaruni, Salah \|4 aut
700	1		\|a Sheils, Orla \|4 aut
700	1		\|a Gallagher, Michael F \|4 aut
700	1		\|a O’Leary, John J \|4 aut
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publishDate	2014
allfields	10.1186/1471-2164-15-711 doi (DE-627)SPR027094103 (SPR)1471-2164-15-711-e DE-627 ger DE-627 rakwb eng Vencken, Sebastian F verfasserin aut An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vencken et al.; licensee BioMed Central Ltd. 2014 Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. SOX2 (dpeaa)DE-He213 microRNA (dpeaa)DE-He213 Embryonic stem cell (dpeaa)DE-He213 Embryonal carcinoma (dpeaa)DE-He213 Pluripotency (dpeaa)DE-He213 EMT (dpeaa)DE-He213 Sethupathy, Praveen aut Blackshields, Gordon aut Spillane, Cathy aut Elbaruni, Salah aut Sheils, Orla aut Gallagher, Michael F aut O’Leary, John J aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 25. Aug. (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:25 month:08 https://dx.doi.org/10.1186/1471-2164-15-711 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 25 08
spelling	10.1186/1471-2164-15-711 doi (DE-627)SPR027094103 (SPR)1471-2164-15-711-e DE-627 ger DE-627 rakwb eng Vencken, Sebastian F verfasserin aut An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vencken et al.; licensee BioMed Central Ltd. 2014 Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. SOX2 (dpeaa)DE-He213 microRNA (dpeaa)DE-He213 Embryonic stem cell (dpeaa)DE-He213 Embryonal carcinoma (dpeaa)DE-He213 Pluripotency (dpeaa)DE-He213 EMT (dpeaa)DE-He213 Sethupathy, Praveen aut Blackshields, Gordon aut Spillane, Cathy aut Elbaruni, Salah aut Sheils, Orla aut Gallagher, Michael F aut O’Leary, John J aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 25. Aug. (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:25 month:08 https://dx.doi.org/10.1186/1471-2164-15-711 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 25 08
allfields_unstemmed	10.1186/1471-2164-15-711 doi (DE-627)SPR027094103 (SPR)1471-2164-15-711-e DE-627 ger DE-627 rakwb eng Vencken, Sebastian F verfasserin aut An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vencken et al.; licensee BioMed Central Ltd. 2014 Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. SOX2 (dpeaa)DE-He213 microRNA (dpeaa)DE-He213 Embryonic stem cell (dpeaa)DE-He213 Embryonal carcinoma (dpeaa)DE-He213 Pluripotency (dpeaa)DE-He213 EMT (dpeaa)DE-He213 Sethupathy, Praveen aut Blackshields, Gordon aut Spillane, Cathy aut Elbaruni, Salah aut Sheils, Orla aut Gallagher, Michael F aut O’Leary, John J aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 25. Aug. (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:25 month:08 https://dx.doi.org/10.1186/1471-2164-15-711 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 25 08
allfieldsGer	10.1186/1471-2164-15-711 doi (DE-627)SPR027094103 (SPR)1471-2164-15-711-e DE-627 ger DE-627 rakwb eng Vencken, Sebastian F verfasserin aut An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vencken et al.; licensee BioMed Central Ltd. 2014 Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. SOX2 (dpeaa)DE-He213 microRNA (dpeaa)DE-He213 Embryonic stem cell (dpeaa)DE-He213 Embryonal carcinoma (dpeaa)DE-He213 Pluripotency (dpeaa)DE-He213 EMT (dpeaa)DE-He213 Sethupathy, Praveen aut Blackshields, Gordon aut Spillane, Cathy aut Elbaruni, Salah aut Sheils, Orla aut Gallagher, Michael F aut O’Leary, John J aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 25. Aug. (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:25 month:08 https://dx.doi.org/10.1186/1471-2164-15-711 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 25 08
allfieldsSound	10.1186/1471-2164-15-711 doi (DE-627)SPR027094103 (SPR)1471-2164-15-711-e DE-627 ger DE-627 rakwb eng Vencken, Sebastian F verfasserin aut An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vencken et al.; licensee BioMed Central Ltd. 2014 Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. SOX2 (dpeaa)DE-He213 microRNA (dpeaa)DE-He213 Embryonic stem cell (dpeaa)DE-He213 Embryonal carcinoma (dpeaa)DE-He213 Pluripotency (dpeaa)DE-He213 EMT (dpeaa)DE-He213 Sethupathy, Praveen aut Blackshields, Gordon aut Spillane, Cathy aut Elbaruni, Salah aut Sheils, Orla aut Gallagher, Michael F aut O’Leary, John J aut Enthalten in BMC genomics London : BioMed Central, 2000 15(2014), 1 vom: 25. Aug. (DE-627)326644954 (DE-600)2041499-7 1471-2164 nnns volume:15 year:2014 number:1 day:25 month:08 https://dx.doi.org/10.1186/1471-2164-15-711 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 25 08
language	English
source	Enthalten in BMC genomics 15(2014), 1 vom: 25. Aug. volume:15 year:2014 number:1 day:25 month:08
sourceStr	Enthalten in BMC genomics 15(2014), 1 vom: 25. Aug. volume:15 year:2014 number:1 day:25 month:08
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	SOX2 microRNA Embryonic stem cell Embryonal carcinoma Pluripotency EMT
isfreeaccess_bool	true
container_title	BMC genomics
authorswithroles_txt_mv	Vencken, Sebastian F @@aut@@ Sethupathy, Praveen @@aut@@ Blackshields, Gordon @@aut@@ Spillane, Cathy @@aut@@ Elbaruni, Salah @@aut@@ Sheils, Orla @@aut@@ Gallagher, Michael F @@aut@@ O’Leary, John J @@aut@@
publishDateDaySort_date	2014-08-25T00:00:00Z
hierarchy_top_id	326644954
id	SPR027094103
language_de	englisch
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As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. 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author	Vencken, Sebastian F
spellingShingle	Vencken, Sebastian F misc SOX2 misc microRNA misc Embryonic stem cell misc Embryonal carcinoma misc Pluripotency misc EMT An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines
authorStr	Vencken, Sebastian F
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topic_title	An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines SOX2 (dpeaa)DE-He213 microRNA (dpeaa)DE-He213 Embryonic stem cell (dpeaa)DE-He213 Embryonal carcinoma (dpeaa)DE-He213 Pluripotency (dpeaa)DE-He213 EMT (dpeaa)DE-He213
topic	misc SOX2 misc microRNA misc Embryonic stem cell misc Embryonal carcinoma misc Pluripotency misc EMT
topic_unstemmed	misc SOX2 misc microRNA misc Embryonic stem cell misc Embryonal carcinoma misc Pluripotency misc EMT
topic_browse	misc SOX2 misc microRNA misc Embryonic stem cell misc Embryonal carcinoma misc Pluripotency misc EMT
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines
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title_full	An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines
author_sort	Vencken, Sebastian F
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author_browse	Vencken, Sebastian F Sethupathy, Praveen Blackshields, Gordon Spillane, Cathy Elbaruni, Salah Sheils, Orla Gallagher, Michael F O’Leary, John J
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author-letter	Vencken, Sebastian F
doi_str_mv	10.1186/1471-2164-15-711
title_sort	integrated analysis of the sox2 microrna response program in human pluripotent and nullipotent stem cell lines
title_auth	An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines
abstract	Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. © Vencken et al.; licensee BioMed Central Ltd. 2014
abstractGer	Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. © Vencken et al.; licensee BioMed Central Ltd. 2014
abstract_unstemmed	Background SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. Results Whole-microRNAome and genome analysis of SOX2-silenced hECCs revealed many miRNAs regulated by SOX2, including several with highly characterised functions in both cancer and embryonic stem cell (ESC) biology. We subsequently performed genome-wide differential expression analysis and applied a Monte Carlo simulation algorithm and target prediction to identify a SOX2-linked miRNA regulome, which was strongly enriched with epithelial-to-mesenchymal transition (EMT) markers. Additionally, several deregulated miRNAs important to EMT processes had SOX2 binding sites in their promoter regions. Conclusion In ESC-like CSCs, SOX2 regulates a large miRNA network that regulates and interlinks the expression of crucial genes involved in EMT. © Vencken et al.; licensee BioMed Central Ltd. 2014
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title_short	An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines
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An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines

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