Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status

Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more pr...
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Autor*in:	Boysen, Preben [verfasserIn] Olsen, Ingrid Berg, Ingvild Kulberg, Siri Johansen, Grethe M Storset, Anne K

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2006

Schlagwörter:	Natural Killer Cell Human Natural Killer Cell Natural Killer Cell Function Natural Killer Cell Receptor Natural Killer Cell Subset

Anmerkung:	© Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC immunology - London : BioMed Central, 2000, 7(2006), 1 vom: 27. Apr.
Übergeordnetes Werk:	volume:7 ; year:2006 ; number:1 ; day:27 ; month:04

Links:	Volltext

DOI / URN:	10.1186/1471-2172-7-10

Katalog-ID:	SPR027164500

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520			\|a Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation.
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912			\|a GBV_ILN_4325
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Indexfelder

author_variant	p b pb i o io i b ib s k sk g m j gm gmj a k s ak aks
matchkey_str	article:14712172:2006----::oiednp6elaeulfntoantrlilrelwta
hierarchy_sort_str	2006
publishDate	2006
allfields	10.1186/1471-2172-7-10 doi (DE-627)SPR027164500 (SPR)1471-2172-7-10-e DE-627 ger DE-627 rakwb eng Boysen, Preben verfasserin aut Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. Natural Killer Cell (dpeaa)DE-He213 Human Natural Killer Cell (dpeaa)DE-He213 Natural Killer Cell Function (dpeaa)DE-He213 Natural Killer Cell Receptor (dpeaa)DE-He213 Natural Killer Cell Subset (dpeaa)DE-He213 Olsen, Ingrid aut Berg, Ingvild aut Kulberg, Siri aut Johansen, Grethe M aut Storset, Anne K aut Enthalten in BMC immunology London : BioMed Central, 2000 7(2006), 1 vom: 27. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:7 year:2006 number:1 day:27 month:04 https://dx.doi.org/10.1186/1471-2172-7-10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 1 27 04
spelling	10.1186/1471-2172-7-10 doi (DE-627)SPR027164500 (SPR)1471-2172-7-10-e DE-627 ger DE-627 rakwb eng Boysen, Preben verfasserin aut Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. Natural Killer Cell (dpeaa)DE-He213 Human Natural Killer Cell (dpeaa)DE-He213 Natural Killer Cell Function (dpeaa)DE-He213 Natural Killer Cell Receptor (dpeaa)DE-He213 Natural Killer Cell Subset (dpeaa)DE-He213 Olsen, Ingrid aut Berg, Ingvild aut Kulberg, Siri aut Johansen, Grethe M aut Storset, Anne K aut Enthalten in BMC immunology London : BioMed Central, 2000 7(2006), 1 vom: 27. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:7 year:2006 number:1 day:27 month:04 https://dx.doi.org/10.1186/1471-2172-7-10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 1 27 04
allfields_unstemmed	10.1186/1471-2172-7-10 doi (DE-627)SPR027164500 (SPR)1471-2172-7-10-e DE-627 ger DE-627 rakwb eng Boysen, Preben verfasserin aut Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. Natural Killer Cell (dpeaa)DE-He213 Human Natural Killer Cell (dpeaa)DE-He213 Natural Killer Cell Function (dpeaa)DE-He213 Natural Killer Cell Receptor (dpeaa)DE-He213 Natural Killer Cell Subset (dpeaa)DE-He213 Olsen, Ingrid aut Berg, Ingvild aut Kulberg, Siri aut Johansen, Grethe M aut Storset, Anne K aut Enthalten in BMC immunology London : BioMed Central, 2000 7(2006), 1 vom: 27. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:7 year:2006 number:1 day:27 month:04 https://dx.doi.org/10.1186/1471-2172-7-10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 1 27 04
allfieldsGer	10.1186/1471-2172-7-10 doi (DE-627)SPR027164500 (SPR)1471-2172-7-10-e DE-627 ger DE-627 rakwb eng Boysen, Preben verfasserin aut Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. Natural Killer Cell (dpeaa)DE-He213 Human Natural Killer Cell (dpeaa)DE-He213 Natural Killer Cell Function (dpeaa)DE-He213 Natural Killer Cell Receptor (dpeaa)DE-He213 Natural Killer Cell Subset (dpeaa)DE-He213 Olsen, Ingrid aut Berg, Ingvild aut Kulberg, Siri aut Johansen, Grethe M aut Storset, Anne K aut Enthalten in BMC immunology London : BioMed Central, 2000 7(2006), 1 vom: 27. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:7 year:2006 number:1 day:27 month:04 https://dx.doi.org/10.1186/1471-2172-7-10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 1 27 04
allfieldsSound	10.1186/1471-2172-7-10 doi (DE-627)SPR027164500 (SPR)1471-2172-7-10-e DE-627 ger DE-627 rakwb eng Boysen, Preben verfasserin aut Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. Natural Killer Cell (dpeaa)DE-He213 Human Natural Killer Cell (dpeaa)DE-He213 Natural Killer Cell Function (dpeaa)DE-He213 Natural Killer Cell Receptor (dpeaa)DE-He213 Natural Killer Cell Subset (dpeaa)DE-He213 Olsen, Ingrid aut Berg, Ingvild aut Kulberg, Siri aut Johansen, Grethe M aut Storset, Anne K aut Enthalten in BMC immunology London : BioMed Central, 2000 7(2006), 1 vom: 27. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:7 year:2006 number:1 day:27 month:04 https://dx.doi.org/10.1186/1471-2172-7-10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2006 1 27 04
language	English
source	Enthalten in BMC immunology 7(2006), 1 vom: 27. Apr. volume:7 year:2006 number:1 day:27 month:04
sourceStr	Enthalten in BMC immunology 7(2006), 1 vom: 27. Apr. volume:7 year:2006 number:1 day:27 month:04
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Natural Killer Cell Human Natural Killer Cell Natural Killer Cell Function Natural Killer Cell Receptor Natural Killer Cell Subset
isfreeaccess_bool	false
container_title	BMC immunology
authorswithroles_txt_mv	Boysen, Preben @@aut@@ Olsen, Ingrid @@aut@@ Berg, Ingvild @@aut@@ Kulberg, Siri @@aut@@ Johansen, Grethe M @@aut@@ Storset, Anne K @@aut@@
publishDateDaySort_date	2006-04-27T00:00:00Z
hierarchy_top_id	326644962
id	SPR027164500
language_de	englisch
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. 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author	Boysen, Preben
spellingShingle	Boysen, Preben misc Natural Killer Cell misc Human Natural Killer Cell misc Natural Killer Cell Function misc Natural Killer Cell Receptor misc Natural Killer Cell Subset Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status
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topic_title	Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status Natural Killer Cell (dpeaa)DE-He213 Human Natural Killer Cell (dpeaa)DE-He213 Natural Killer Cell Function (dpeaa)DE-He213 Natural Killer Cell Receptor (dpeaa)DE-He213 Natural Killer Cell Subset (dpeaa)DE-He213
topic	misc Natural Killer Cell misc Human Natural Killer Cell misc Natural Killer Cell Function misc Natural Killer Cell Receptor misc Natural Killer Cell Subset
topic_unstemmed	misc Natural Killer Cell misc Human Natural Killer Cell misc Natural Killer Cell Function misc Natural Killer Cell Receptor misc Natural Killer Cell Subset
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title	Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status
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title_full	Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status
author_sort	Boysen, Preben
journal	BMC immunology
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lang_code	eng
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author_browse	Boysen, Preben Olsen, Ingrid Berg, Ingvild Kulberg, Siri Johansen, Grethe M Storset, Anne K
container_volume	7
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doi_str_mv	10.1186/1471-2172-7-10
title_sort	bovine $ cd2^{-} $/$ nkp46^{+} $ cells are fully functional natural killer cells with a high activation status
title_auth	Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status
abstract	Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Background Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a $ CD3^{-} $/$ CD2^{+} $ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2. Results Bovine $ CD2^{-} $ NK cells, a minor subset in blood, proliferated more rapidly in the presence of IL-2, dominating the cultures after a few days. Grown separately with IL-2, $ CD2^{-} $ and $ CD2^{+} $ NK cell subsets did not change CD2 expression for at least two weeks. In blood, $ CD2^{-} $ NK cells showed a higher expression of CD44 and CD25, consistent with a high activation status. A higher proportion of $ CD2^{-} $ NK cells had intracellular interferon-gamma in the cytoplasm in response to IL-2 and IL-12 stimulation, and the $ CD2^{-} $ subset secreted more interferon-gamma when cultured separately. Cytotoxic capacity was similar in both subsets, and both carried transcripts for the NK cell receptors KIR, CD16, CD94 and KLRJ. Ligation by one out of two tested anti-CD2 monoclonal antibodies could trigger interferon-gamma production from NK cells, but neither of them could alter cytotoxicity. Conclusion These results provide evidence that bovine $ CD2^{-} $ as well as $ CD2^{+} $ cells of the $ NKp46^{+} $ phenotype are fully functional NK cells, the $ CD2^{-} $ subset showing signs of being more activated in the circulation. © Boysen et al; licensee BioMed Central Ltd. 2006. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status
url	https://dx.doi.org/10.1186/1471-2172-7-10
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author2	Olsen, Ingrid Berg, Ingvild Kulberg, Siri Johansen, Grethe M Storset, Anne K
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Bovine $ CD2^{-} $/$ NKp46^{+} $ cells are fully functional natural killer cells with a high activation status

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