Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva

Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania br...
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Autor*in:	Menezes, Maria José [verfasserIn] Costa, Dirceu J Clarêncio, Jorge Miranda, José Carlos Barral, Aldina Barral-Netto, Manoel Brodskyn, Cláudia de Oliveira, Camila I

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Schlagwörter:	Leishmaniasis Human Monocyte Cutaneous Leishmaniasis Infected Monocyte Mucosal Leishmaniasis

Anmerkung:	© Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC immunology - London : BioMed Central, 2000, 9(2008), 1 vom: 10. Apr.
Übergeordnetes Werk:	volume:9 ; year:2008 ; number:1 ; day:10 ; month:04

Links:	Volltext

DOI / URN:	10.1186/1471-2172-9-12

Katalog-ID:	SPR027165019

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520			\|a Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism.
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allfields	10.1186/1471-2172-9-12 doi (DE-627)SPR027165019 (SPR)1471-2172-9-12-e DE-627 ger DE-627 rakwb eng Menezes, Maria José verfasserin aut Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. Leishmaniasis (dpeaa)DE-He213 Human Monocyte (dpeaa)DE-He213 Cutaneous Leishmaniasis (dpeaa)DE-He213 Infected Monocyte (dpeaa)DE-He213 Mucosal Leishmaniasis (dpeaa)DE-He213 Costa, Dirceu J aut Clarêncio, Jorge aut Miranda, José Carlos aut Barral, Aldina aut Barral-Netto, Manoel aut Brodskyn, Cláudia aut de Oliveira, Camila I aut Enthalten in BMC immunology London : BioMed Central, 2000 9(2008), 1 vom: 10. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:9 year:2008 number:1 day:10 month:04 https://dx.doi.org/10.1186/1471-2172-9-12 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1 10 04
spelling	10.1186/1471-2172-9-12 doi (DE-627)SPR027165019 (SPR)1471-2172-9-12-e DE-627 ger DE-627 rakwb eng Menezes, Maria José verfasserin aut Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. Leishmaniasis (dpeaa)DE-He213 Human Monocyte (dpeaa)DE-He213 Cutaneous Leishmaniasis (dpeaa)DE-He213 Infected Monocyte (dpeaa)DE-He213 Mucosal Leishmaniasis (dpeaa)DE-He213 Costa, Dirceu J aut Clarêncio, Jorge aut Miranda, José Carlos aut Barral, Aldina aut Barral-Netto, Manoel aut Brodskyn, Cláudia aut de Oliveira, Camila I aut Enthalten in BMC immunology London : BioMed Central, 2000 9(2008), 1 vom: 10. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:9 year:2008 number:1 day:10 month:04 https://dx.doi.org/10.1186/1471-2172-9-12 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1 10 04
allfields_unstemmed	10.1186/1471-2172-9-12 doi (DE-627)SPR027165019 (SPR)1471-2172-9-12-e DE-627 ger DE-627 rakwb eng Menezes, Maria José verfasserin aut Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. Leishmaniasis (dpeaa)DE-He213 Human Monocyte (dpeaa)DE-He213 Cutaneous Leishmaniasis (dpeaa)DE-He213 Infected Monocyte (dpeaa)DE-He213 Mucosal Leishmaniasis (dpeaa)DE-He213 Costa, Dirceu J aut Clarêncio, Jorge aut Miranda, José Carlos aut Barral, Aldina aut Barral-Netto, Manoel aut Brodskyn, Cláudia aut de Oliveira, Camila I aut Enthalten in BMC immunology London : BioMed Central, 2000 9(2008), 1 vom: 10. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:9 year:2008 number:1 day:10 month:04 https://dx.doi.org/10.1186/1471-2172-9-12 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1 10 04
allfieldsGer	10.1186/1471-2172-9-12 doi (DE-627)SPR027165019 (SPR)1471-2172-9-12-e DE-627 ger DE-627 rakwb eng Menezes, Maria José verfasserin aut Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. Leishmaniasis (dpeaa)DE-He213 Human Monocyte (dpeaa)DE-He213 Cutaneous Leishmaniasis (dpeaa)DE-He213 Infected Monocyte (dpeaa)DE-He213 Mucosal Leishmaniasis (dpeaa)DE-He213 Costa, Dirceu J aut Clarêncio, Jorge aut Miranda, José Carlos aut Barral, Aldina aut Barral-Netto, Manoel aut Brodskyn, Cláudia aut de Oliveira, Camila I aut Enthalten in BMC immunology London : BioMed Central, 2000 9(2008), 1 vom: 10. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:9 year:2008 number:1 day:10 month:04 https://dx.doi.org/10.1186/1471-2172-9-12 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1 10 04
allfieldsSound	10.1186/1471-2172-9-12 doi (DE-627)SPR027165019 (SPR)1471-2172-9-12-e DE-627 ger DE-627 rakwb eng Menezes, Maria José verfasserin aut Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. Leishmaniasis (dpeaa)DE-He213 Human Monocyte (dpeaa)DE-He213 Cutaneous Leishmaniasis (dpeaa)DE-He213 Infected Monocyte (dpeaa)DE-He213 Mucosal Leishmaniasis (dpeaa)DE-He213 Costa, Dirceu J aut Clarêncio, Jorge aut Miranda, José Carlos aut Barral, Aldina aut Barral-Netto, Manoel aut Brodskyn, Cláudia aut de Oliveira, Camila I aut Enthalten in BMC immunology London : BioMed Central, 2000 9(2008), 1 vom: 10. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:9 year:2008 number:1 day:10 month:04 https://dx.doi.org/10.1186/1471-2172-9-12 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1 10 04
language	English
source	Enthalten in BMC immunology 9(2008), 1 vom: 10. Apr. volume:9 year:2008 number:1 day:10 month:04
sourceStr	Enthalten in BMC immunology 9(2008), 1 vom: 10. Apr. volume:9 year:2008 number:1 day:10 month:04
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Leishmaniasis Human Monocyte Cutaneous Leishmaniasis Infected Monocyte Mucosal Leishmaniasis
isfreeaccess_bool	false
container_title	BMC immunology
authorswithroles_txt_mv	Menezes, Maria José @@aut@@ Costa, Dirceu J @@aut@@ Clarêncio, Jorge @@aut@@ Miranda, José Carlos @@aut@@ Barral, Aldina @@aut@@ Barral-Netto, Manoel @@aut@@ Brodskyn, Cláudia @@aut@@ de Oliveira, Camila I @@aut@@
publishDateDaySort_date	2008-04-10T00:00:00Z
hierarchy_top_id	326644962
id	SPR027165019
language_de	englisch
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author	Menezes, Maria José
spellingShingle	Menezes, Maria José misc Leishmaniasis misc Human Monocyte misc Cutaneous Leishmaniasis misc Infected Monocyte misc Mucosal Leishmaniasis Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
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topic_title	Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva Leishmaniasis (dpeaa)DE-He213 Human Monocyte (dpeaa)DE-He213 Cutaneous Leishmaniasis (dpeaa)DE-He213 Infected Monocyte (dpeaa)DE-He213 Mucosal Leishmaniasis (dpeaa)DE-He213
topic	misc Leishmaniasis misc Human Monocyte misc Cutaneous Leishmaniasis misc Infected Monocyte misc Mucosal Leishmaniasis
topic_unstemmed	misc Leishmaniasis misc Human Monocyte misc Cutaneous Leishmaniasis misc Infected Monocyte misc Mucosal Leishmaniasis
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title	Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
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title_full	Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
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author_browse	Menezes, Maria José Costa, Dirceu J Clarêncio, Jorge Miranda, José Carlos Barral, Aldina Barral-Netto, Manoel Brodskyn, Cláudia de Oliveira, Camila I
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title_sort	immunomodulation of human monocytes following exposure to lutzomyia intermedia saliva
title_auth	Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
abstract	Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Background Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis. Results Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-α, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes. Conclusion Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism. © Menezes et al; licensee BioMed Central Ltd. 2008. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
url	https://dx.doi.org/10.1186/1471-2172-9-12
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author2	Costa, Dirceu J Clarêncio, Jorge Miranda, José Carlos Barral, Aldina Barral-Netto, Manoel Brodskyn, Cláudia de Oliveira, Camila I
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Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva

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