An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis
Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of prim...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tsurikisawa, Naomi [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2014 |
|---|
| Schlagwörter: |
|---|
| Anmerkung: |
© Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 |
|---|
| Übergeordnetes Werk: |
Enthalten in: BMC immunology - London : BioMed Central, 2000, 15(2014), 1 vom: 31. Aug. |
|---|---|
| Übergeordnetes Werk: |
volume:15 ; year:2014 ; number:1 ; day:31 ; month:08 |
| Links: |
|---|
| DOI / URN: |
10.1186/s12865-014-0032-5 |
|---|
| Katalog-ID: |
SPR027169553 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR027169553 | ||
| 003 | DE-627 | ||
| 005 | 20230519233133.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201007s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1186/s12865-014-0032-5 |2 doi | |
| 035 | |a (DE-627)SPR027169553 | ||
| 035 | |a (SPR)s12865-014-0032-5-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Tsurikisawa, Naomi |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis |
| 264 | 1 | |c 2014 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 | ||
| 520 | |a Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. | ||
| 650 | 4 | |a Churg–Strauss syndrome |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Dendritic cell |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Eosinophilic granulomatosis with polyangiitis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a EGPA |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Regulatory T cell |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Saito, Hiroshi |4 aut | |
| 700 | 1 | |a Oshikata, Chiyako |4 aut | |
| 700 | 1 | |a Tsuburai, Takahiro |4 aut | |
| 700 | 1 | |a Ishiyama, Miyako |4 aut | |
| 700 | 1 | |a Mitomi, Hiroyuki |4 aut | |
| 700 | 1 | |a Akiyama, Kazuo |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t BMC immunology |d London : BioMed Central, 2000 |g 15(2014), 1 vom: 31. Aug. |w (DE-627)326644962 |w (DE-600)2041500-X |x 1471-2172 |7 nnns |
| 773 | 1 | 8 | |g volume:15 |g year:2014 |g number:1 |g day:31 |g month:08 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1186/s12865-014-0032-5 |z kostenfrei |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a GBV_ILN_20 | ||
| 912 | |a GBV_ILN_22 | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_39 | ||
| 912 | |a GBV_ILN_40 | ||
| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_65 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_206 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2056 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4367 | ||
| 912 | |a GBV_ILN_4700 | ||
| 951 | |a AR | ||
| 952 | |d 15 |j 2014 |e 1 |b 31 |c 08 | ||
| author_variant |
n t nt h s hs c o co t t tt m i mi h m hm k a ka |
|---|---|
| matchkey_str |
article:14712172:2014----::nnraefd3ediiclsxioorltsihnraergltrteliptetwtatvesnp |
| hierarchy_sort_str |
2014 |
| publishDate |
2014 |
| allfields |
10.1186/s12865-014-0032-5 doi (DE-627)SPR027169553 (SPR)s12865-014-0032-5-e DE-627 ger DE-627 rakwb eng Tsurikisawa, Naomi verfasserin aut An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. Churg–Strauss syndrome (dpeaa)DE-He213 Dendritic cell (dpeaa)DE-He213 Eosinophilic granulomatosis with polyangiitis (dpeaa)DE-He213 EGPA (dpeaa)DE-He213 Regulatory T cell (dpeaa)DE-He213 Saito, Hiroshi aut Oshikata, Chiyako aut Tsuburai, Takahiro aut Ishiyama, Miyako aut Mitomi, Hiroyuki aut Akiyama, Kazuo aut Enthalten in BMC immunology London : BioMed Central, 2000 15(2014), 1 vom: 31. Aug. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:15 year:2014 number:1 day:31 month:08 https://dx.doi.org/10.1186/s12865-014-0032-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 31 08 |
| spelling |
10.1186/s12865-014-0032-5 doi (DE-627)SPR027169553 (SPR)s12865-014-0032-5-e DE-627 ger DE-627 rakwb eng Tsurikisawa, Naomi verfasserin aut An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. Churg–Strauss syndrome (dpeaa)DE-He213 Dendritic cell (dpeaa)DE-He213 Eosinophilic granulomatosis with polyangiitis (dpeaa)DE-He213 EGPA (dpeaa)DE-He213 Regulatory T cell (dpeaa)DE-He213 Saito, Hiroshi aut Oshikata, Chiyako aut Tsuburai, Takahiro aut Ishiyama, Miyako aut Mitomi, Hiroyuki aut Akiyama, Kazuo aut Enthalten in BMC immunology London : BioMed Central, 2000 15(2014), 1 vom: 31. Aug. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:15 year:2014 number:1 day:31 month:08 https://dx.doi.org/10.1186/s12865-014-0032-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 31 08 |
| allfields_unstemmed |
10.1186/s12865-014-0032-5 doi (DE-627)SPR027169553 (SPR)s12865-014-0032-5-e DE-627 ger DE-627 rakwb eng Tsurikisawa, Naomi verfasserin aut An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. Churg–Strauss syndrome (dpeaa)DE-He213 Dendritic cell (dpeaa)DE-He213 Eosinophilic granulomatosis with polyangiitis (dpeaa)DE-He213 EGPA (dpeaa)DE-He213 Regulatory T cell (dpeaa)DE-He213 Saito, Hiroshi aut Oshikata, Chiyako aut Tsuburai, Takahiro aut Ishiyama, Miyako aut Mitomi, Hiroyuki aut Akiyama, Kazuo aut Enthalten in BMC immunology London : BioMed Central, 2000 15(2014), 1 vom: 31. Aug. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:15 year:2014 number:1 day:31 month:08 https://dx.doi.org/10.1186/s12865-014-0032-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 31 08 |
| allfieldsGer |
10.1186/s12865-014-0032-5 doi (DE-627)SPR027169553 (SPR)s12865-014-0032-5-e DE-627 ger DE-627 rakwb eng Tsurikisawa, Naomi verfasserin aut An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. Churg–Strauss syndrome (dpeaa)DE-He213 Dendritic cell (dpeaa)DE-He213 Eosinophilic granulomatosis with polyangiitis (dpeaa)DE-He213 EGPA (dpeaa)DE-He213 Regulatory T cell (dpeaa)DE-He213 Saito, Hiroshi aut Oshikata, Chiyako aut Tsuburai, Takahiro aut Ishiyama, Miyako aut Mitomi, Hiroyuki aut Akiyama, Kazuo aut Enthalten in BMC immunology London : BioMed Central, 2000 15(2014), 1 vom: 31. Aug. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:15 year:2014 number:1 day:31 month:08 https://dx.doi.org/10.1186/s12865-014-0032-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 31 08 |
| allfieldsSound |
10.1186/s12865-014-0032-5 doi (DE-627)SPR027169553 (SPR)s12865-014-0032-5-e DE-627 ger DE-627 rakwb eng Tsurikisawa, Naomi verfasserin aut An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. Churg–Strauss syndrome (dpeaa)DE-He213 Dendritic cell (dpeaa)DE-He213 Eosinophilic granulomatosis with polyangiitis (dpeaa)DE-He213 EGPA (dpeaa)DE-He213 Regulatory T cell (dpeaa)DE-He213 Saito, Hiroshi aut Oshikata, Chiyako aut Tsuburai, Takahiro aut Ishiyama, Miyako aut Mitomi, Hiroyuki aut Akiyama, Kazuo aut Enthalten in BMC immunology London : BioMed Central, 2000 15(2014), 1 vom: 31. Aug. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:15 year:2014 number:1 day:31 month:08 https://dx.doi.org/10.1186/s12865-014-0032-5 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 1 31 08 |
| language |
English |
| source |
Enthalten in BMC immunology 15(2014), 1 vom: 31. Aug. volume:15 year:2014 number:1 day:31 month:08 |
| sourceStr |
Enthalten in BMC immunology 15(2014), 1 vom: 31. Aug. volume:15 year:2014 number:1 day:31 month:08 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Churg–Strauss syndrome Dendritic cell Eosinophilic granulomatosis with polyangiitis EGPA Regulatory T cell |
| isfreeaccess_bool |
true |
| container_title |
BMC immunology |
| authorswithroles_txt_mv |
Tsurikisawa, Naomi @@aut@@ Saito, Hiroshi @@aut@@ Oshikata, Chiyako @@aut@@ Tsuburai, Takahiro @@aut@@ Ishiyama, Miyako @@aut@@ Mitomi, Hiroyuki @@aut@@ Akiyama, Kazuo @@aut@@ |
| publishDateDaySort_date |
2014-08-31T00:00:00Z |
| hierarchy_top_id |
326644962 |
| id |
SPR027169553 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR027169553</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519233133.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2014 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s12865-014-0032-5</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR027169553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12865-014-0032-5-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Tsurikisawa, Naomi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="3"><subfield code="a">An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Tsurikisawa et al.; licensee BioMed Central Ltd. 2014</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Churg–Strauss syndrome</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dendritic cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Eosinophilic granulomatosis with polyangiitis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">EGPA</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Regulatory T cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Saito, Hiroshi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Oshikata, Chiyako</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tsuburai, Takahiro</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ishiyama, Miyako</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mitomi, Hiroyuki</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Akiyama, Kazuo</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">BMC immunology</subfield><subfield code="d">London : BioMed Central, 2000</subfield><subfield code="g">15(2014), 1 vom: 31. Aug.</subfield><subfield code="w">(DE-627)326644962</subfield><subfield code="w">(DE-600)2041500-X</subfield><subfield code="x">1471-2172</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:15</subfield><subfield code="g">year:2014</subfield><subfield code="g">number:1</subfield><subfield code="g">day:31</subfield><subfield code="g">month:08</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1186/s12865-014-0032-5</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">15</subfield><subfield code="j">2014</subfield><subfield code="e">1</subfield><subfield code="b">31</subfield><subfield code="c">08</subfield></datafield></record></collection>
|
| author |
Tsurikisawa, Naomi |
| spellingShingle |
Tsurikisawa, Naomi misc Churg–Strauss syndrome misc Dendritic cell misc Eosinophilic granulomatosis with polyangiitis misc EGPA misc Regulatory T cell An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis |
| authorStr |
Tsurikisawa, Naomi |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)326644962 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut aut aut aut |
| collection |
springer |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1471-2172 |
| topic_title |
An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis Churg–Strauss syndrome (dpeaa)DE-He213 Dendritic cell (dpeaa)DE-He213 Eosinophilic granulomatosis with polyangiitis (dpeaa)DE-He213 EGPA (dpeaa)DE-He213 Regulatory T cell (dpeaa)DE-He213 |
| topic |
misc Churg–Strauss syndrome misc Dendritic cell misc Eosinophilic granulomatosis with polyangiitis misc EGPA misc Regulatory T cell |
| topic_unstemmed |
misc Churg–Strauss syndrome misc Dendritic cell misc Eosinophilic granulomatosis with polyangiitis misc EGPA misc Regulatory T cell |
| topic_browse |
misc Churg–Strauss syndrome misc Dendritic cell misc Eosinophilic granulomatosis with polyangiitis misc EGPA misc Regulatory T cell |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
BMC immunology |
| hierarchy_parent_id |
326644962 |
| hierarchy_top_title |
BMC immunology |
| isfreeaccess_txt |
true |
| familylinks_str_mv |
(DE-627)326644962 (DE-600)2041500-X |
| title |
An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis |
| ctrlnum |
(DE-627)SPR027169553 (SPR)s12865-014-0032-5-e |
| title_full |
An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis |
| author_sort |
Tsurikisawa, Naomi |
| journal |
BMC immunology |
| journalStr |
BMC immunology |
| lang_code |
eng |
| isOA_bool |
true |
| recordtype |
marc |
| publishDateSort |
2014 |
| contenttype_str_mv |
txt |
| author_browse |
Tsurikisawa, Naomi Saito, Hiroshi Oshikata, Chiyako Tsuburai, Takahiro Ishiyama, Miyako Mitomi, Hiroyuki Akiyama, Kazuo |
| container_volume |
15 |
| format_se |
Elektronische Aufsätze |
| author-letter |
Tsurikisawa, Naomi |
| doi_str_mv |
10.1186/s12865-014-0032-5 |
| title_sort |
increase of $ cd83^{+} $ dendritic cells ex vivo correlates with increased regulatory t cells in patients with active eosinophilic granulomatosis and polyangiitis |
| title_auth |
An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis |
| abstract |
Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 |
| abstractGer |
Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 |
| abstract_unstemmed |
Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity. © Tsurikisawa et al.; licensee BioMed Central Ltd. 2014 |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 |
| container_issue |
1 |
| title_short |
An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis |
| url |
https://dx.doi.org/10.1186/s12865-014-0032-5 |
| remote_bool |
true |
| author2 |
Saito, Hiroshi Oshikata, Chiyako Tsuburai, Takahiro Ishiyama, Miyako Mitomi, Hiroyuki Akiyama, Kazuo |
| author2Str |
Saito, Hiroshi Oshikata, Chiyako Tsuburai, Takahiro Ishiyama, Miyako Mitomi, Hiroyuki Akiyama, Kazuo |
| ppnlink |
326644962 |
| mediatype_str_mv |
c |
| isOA_txt |
true |
| hochschulschrift_bool |
false |
| doi_str |
10.1186/s12865-014-0032-5 |
| up_date |
2024-07-04T00:42:27.809Z |
| _version_ |
1803607078663618560 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR027169553</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519233133.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2014 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s12865-014-0032-5</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR027169553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12865-014-0032-5-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Tsurikisawa, Naomi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="3"><subfield code="a">An increase of $ CD83^{+} $ dendritic cells ex vivo correlates with increased regulatory T cells in patients with active eosinophilic granulomatosis and polyangiitis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Tsurikisawa et al.; licensee BioMed Central Ltd. 2014</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by the presence of allergic granulomatosis and necrotizing vasculitis with eosinophilic infiltration. The etiology of EGPA is unknown. Dendritic cells (DCs) are not only critical for the induction of primary immune responses; they may also be important for the induction of immunological tolerance and the regulation of the type of T-cell-mediated immune response. To investigate whether DC maturation is associated with EGPA disease status, we examined the relationship between the maturation of DCs and the differentiation of regulatory T ($ T_{reg} $) cells in EGPA patients. We exposed the $ CD14^{+} $ blood monocytes of 19 patients with EGPA in remission or relapse to stimulation with GM-CSF and IL-4 for 6 d and lipopolysaccharide for 24 h to obtain mature $ CD83^{+} $ DCs and immature $ CD206^{+} $ DCs. Using immunohistochemistry, we examined four patients for the presence of $ CD83^{+} $ and $ CD206^{+} $ DCs in the lung at the onset of EGPA. Results The percentage of $ CD83^{+} $ cells among DCs differentiated from $ CD14^{+} $ monocytes was lower for EGPA patients in relapse than in remission. The percentage of $ CD83^{+} $ DCs was inversely correlated with the percentage of $ CD206^{+} $ DCs and was significantly correlated with the numbers of naturally occurring $ CD4^{+} $ regulatory $ T_{reg} $ ($ nT_{reg} $; $ FOXP3^{+} %$ CD4^{+} $) cells and inducible Treg (iTreg; $ CD4^{+} %$ CD25^{+} $ T cells producing IL-10 or TGF-β) cells but not the number of eosinophils. The percentage of $ CD206^{+} $ DCs was significantly inversely correlated with the percentages of $ nT_{reg} $ and iTreg cells but not the number of eosinophils. Immunohistochemistry revealed both $ CD206^{+} $ DCs and $ CD83^{+} $ DCs in alveoli and interstitial spaces at the onset of EGPA. Conclusion The maturation of DCs from monocytes was related to disease activity in patients with EGPA. Increased $ CD83^{+} $ DCs in EGPA patients may induce the differentiation of $ iT_{reg} $ and $ nT_{reg} $ cells, thereby suppressing inflammation and disease activity.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Churg–Strauss syndrome</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dendritic cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Eosinophilic granulomatosis with polyangiitis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">EGPA</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Regulatory T cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Saito, Hiroshi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Oshikata, Chiyako</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tsuburai, Takahiro</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ishiyama, Miyako</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mitomi, Hiroyuki</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Akiyama, Kazuo</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">BMC immunology</subfield><subfield code="d">London : BioMed Central, 2000</subfield><subfield code="g">15(2014), 1 vom: 31. Aug.</subfield><subfield code="w">(DE-627)326644962</subfield><subfield code="w">(DE-600)2041500-X</subfield><subfield code="x">1471-2172</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:15</subfield><subfield code="g">year:2014</subfield><subfield code="g">number:1</subfield><subfield code="g">day:31</subfield><subfield code="g">month:08</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1186/s12865-014-0032-5</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">15</subfield><subfield code="j">2014</subfield><subfield code="e">1</subfield><subfield code="b">31</subfield><subfield code="c">08</subfield></datafield></record></collection>
|
| score |
7.400996 |