The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029

Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprise...
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Autor*in:	Vandahl, Brian Berg [verfasserIn] Pedersen, Anna Sofie Gevaert, Kris Holm, Arne Vandekerckhove, Joël Christiansen, Gunna Birkelund, Svend

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2002

Schlagwörter:	Cleavage Site Protein Spot Formalin Fixation Signal Peptidase Developmental Cycle

Anmerkung:	© Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Übergeordnetes Werk:	Enthalten in: BMC microbiology - London : BioMed Central, 2001, 2(2002), 1 vom: 26. Nov.
Übergeordnetes Werk:	volume:2 ; year:2002 ; number:1 ; day:26 ; month:11

Links:	Volltext

DOI / URN:	10.1186/1471-2180-2-36

Katalog-ID:	SPR027174018

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245	1	4	\|a The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029
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520			\|a Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters.
650		4	\|a Cleavage Site \|7 (dpeaa)DE-He213
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650		4	\|a Signal Peptidase \|7 (dpeaa)DE-He213
650		4	\|a Developmental Cycle \|7 (dpeaa)DE-He213
700	1		\|a Pedersen, Anna Sofie \|4 aut
700	1		\|a Gevaert, Kris \|4 aut
700	1		\|a Holm, Arne \|4 aut
700	1		\|a Vandekerckhove, Joël \|4 aut
700	1		\|a Christiansen, Gunna \|4 aut
700	1		\|a Birkelund, Svend \|4 aut
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912			\|a GBV_USEFLAG_A
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912			\|a GBV_ILN_22
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912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
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912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
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author_variant	b b v bb bbv a s p as asp k g kg a h ah j v jv g c gc s b sb
matchkey_str	article:14712180:2002----::hepesopoesnadoaiainfoyopimmrnpoeniclm
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publishDate	2002
allfields	10.1186/1471-2180-2-36 doi (DE-627)SPR027174018 (SPR)1471-2180-2-36-e DE-627 ger DE-627 rakwb eng Vandahl, Brian Berg verfasserin aut The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. Cleavage Site (dpeaa)DE-He213 Protein Spot (dpeaa)DE-He213 Formalin Fixation (dpeaa)DE-He213 Signal Peptidase (dpeaa)DE-He213 Developmental Cycle (dpeaa)DE-He213 Pedersen, Anna Sofie aut Gevaert, Kris aut Holm, Arne aut Vandekerckhove, Joël aut Christiansen, Gunna aut Birkelund, Svend aut Enthalten in BMC microbiology London : BioMed Central, 2001 2(2002), 1 vom: 26. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:2 year:2002 number:1 day:26 month:11 https://dx.doi.org/10.1186/1471-2180-2-36 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1 26 11
spelling	10.1186/1471-2180-2-36 doi (DE-627)SPR027174018 (SPR)1471-2180-2-36-e DE-627 ger DE-627 rakwb eng Vandahl, Brian Berg verfasserin aut The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. Cleavage Site (dpeaa)DE-He213 Protein Spot (dpeaa)DE-He213 Formalin Fixation (dpeaa)DE-He213 Signal Peptidase (dpeaa)DE-He213 Developmental Cycle (dpeaa)DE-He213 Pedersen, Anna Sofie aut Gevaert, Kris aut Holm, Arne aut Vandekerckhove, Joël aut Christiansen, Gunna aut Birkelund, Svend aut Enthalten in BMC microbiology London : BioMed Central, 2001 2(2002), 1 vom: 26. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:2 year:2002 number:1 day:26 month:11 https://dx.doi.org/10.1186/1471-2180-2-36 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1 26 11
allfields_unstemmed	10.1186/1471-2180-2-36 doi (DE-627)SPR027174018 (SPR)1471-2180-2-36-e DE-627 ger DE-627 rakwb eng Vandahl, Brian Berg verfasserin aut The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. Cleavage Site (dpeaa)DE-He213 Protein Spot (dpeaa)DE-He213 Formalin Fixation (dpeaa)DE-He213 Signal Peptidase (dpeaa)DE-He213 Developmental Cycle (dpeaa)DE-He213 Pedersen, Anna Sofie aut Gevaert, Kris aut Holm, Arne aut Vandekerckhove, Joël aut Christiansen, Gunna aut Birkelund, Svend aut Enthalten in BMC microbiology London : BioMed Central, 2001 2(2002), 1 vom: 26. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:2 year:2002 number:1 day:26 month:11 https://dx.doi.org/10.1186/1471-2180-2-36 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1 26 11
allfieldsGer	10.1186/1471-2180-2-36 doi (DE-627)SPR027174018 (SPR)1471-2180-2-36-e DE-627 ger DE-627 rakwb eng Vandahl, Brian Berg verfasserin aut The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. Cleavage Site (dpeaa)DE-He213 Protein Spot (dpeaa)DE-He213 Formalin Fixation (dpeaa)DE-He213 Signal Peptidase (dpeaa)DE-He213 Developmental Cycle (dpeaa)DE-He213 Pedersen, Anna Sofie aut Gevaert, Kris aut Holm, Arne aut Vandekerckhove, Joël aut Christiansen, Gunna aut Birkelund, Svend aut Enthalten in BMC microbiology London : BioMed Central, 2001 2(2002), 1 vom: 26. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:2 year:2002 number:1 day:26 month:11 https://dx.doi.org/10.1186/1471-2180-2-36 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1 26 11
allfieldsSound	10.1186/1471-2180-2-36 doi (DE-627)SPR027174018 (SPR)1471-2180-2-36-e DE-627 ger DE-627 rakwb eng Vandahl, Brian Berg verfasserin aut The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. Cleavage Site (dpeaa)DE-He213 Protein Spot (dpeaa)DE-He213 Formalin Fixation (dpeaa)DE-He213 Signal Peptidase (dpeaa)DE-He213 Developmental Cycle (dpeaa)DE-He213 Pedersen, Anna Sofie aut Gevaert, Kris aut Holm, Arne aut Vandekerckhove, Joël aut Christiansen, Gunna aut Birkelund, Svend aut Enthalten in BMC microbiology London : BioMed Central, 2001 2(2002), 1 vom: 26. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:2 year:2002 number:1 day:26 month:11 https://dx.doi.org/10.1186/1471-2180-2-36 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1 26 11
language	English
source	Enthalten in BMC microbiology 2(2002), 1 vom: 26. Nov. volume:2 year:2002 number:1 day:26 month:11
sourceStr	Enthalten in BMC microbiology 2(2002), 1 vom: 26. Nov. volume:2 year:2002 number:1 day:26 month:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Cleavage Site Protein Spot Formalin Fixation Signal Peptidase Developmental Cycle
isfreeaccess_bool	true
container_title	BMC microbiology
authorswithroles_txt_mv	Vandahl, Brian Berg @@aut@@ Pedersen, Anna Sofie @@aut@@ Gevaert, Kris @@aut@@ Holm, Arne @@aut@@ Vandekerckhove, Joël @@aut@@ Christiansen, Gunna @@aut@@ Birkelund, Svend @@aut@@
publishDateDaySort_date	2002-11-26T00:00:00Z
hierarchy_top_id	326644997
id	SPR027174018
language_de	englisch
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author	Vandahl, Brian Berg
spellingShingle	Vandahl, Brian Berg misc Cleavage Site misc Protein Spot misc Formalin Fixation misc Signal Peptidase misc Developmental Cycle The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029
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topic_title	The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029 Cleavage Site (dpeaa)DE-He213 Protein Spot (dpeaa)DE-He213 Formalin Fixation (dpeaa)DE-He213 Signal Peptidase (dpeaa)DE-He213 Developmental Cycle (dpeaa)DE-He213
topic	misc Cleavage Site misc Protein Spot misc Formalin Fixation misc Signal Peptidase misc Developmental Cycle
topic_unstemmed	misc Cleavage Site misc Protein Spot misc Formalin Fixation misc Signal Peptidase misc Developmental Cycle
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title	The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029
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title_full	The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029
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author_browse	Vandahl, Brian Berg Pedersen, Anna Sofie Gevaert, Kris Holm, Arne Vandekerckhove, Joël Christiansen, Gunna Birkelund, Svend
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author-letter	Vandahl, Brian Berg
doi_str_mv	10.1186/1471-2180-2-36
title_sort	expression, processing and localization of polymorphic membrane proteins in chlamydia pneumoniae strain cwl029
title_auth	The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029
abstract	Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
abstractGer	Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
abstract_unstemmed	Background Chlamydiae are obligate intracellular bacteria, which are important human pathogens. Genome sequences of C. trachomatis and C. pneumoniae have revealed the presence of a Chlamydia specific gene family encoding polymorphic outer membrane proteins, Pmps. In C. pneumoniae the family comprises twenty-one members, which are all transcribed. In the present study, the expression, processing and localisation of the sixteen full-length Pmps in C. pneumoniae strain CWL029 have been further investigated by two-dimensional gel electrophoresis and immunofluorescence microscopy. Results Ten Pmps were identified in elementary bodies (EBs). Eight of these were investigated with respect to time dependent expression and all were found to be up-regulated between 36 and 48 hours post infection. Antibodies against Pmp6, 8, 10, 11 and 21 reacted with chlamydiae when infected cells were formalin fixed. Pmp6, Pmp20 and Pmp21 were found in cleaved forms, and the cleavage sites of Pmp6 and Pmp21 were identified. Conclusions The Pmps are heavily up-regulated at the time of conversion of RB to EB, and at least ten Pmps are present in EBs. Due to their reaction in formalin fixation it is likely that Pmp6, 8, 10, 11 and 21 are surface exposed. The identified cleavage sites of Pmp6 and Pmp21 are in agreement with the theory that the Pmps are autotransporters. © Vandahl et al; licensee BioMed Central Ltd. 2002. This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
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title_short	The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029
url	https://dx.doi.org/10.1186/1471-2180-2-36
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author2	Pedersen, Anna Sofie Gevaert, Kris Holm, Arne Vandekerckhove, Joël Christiansen, Gunna Birkelund, Svend
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The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029

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