First insight into Mycobacterium tuberculosis genetic diversity in Paraguay

Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified...
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Autor*in:	Candia, Norma [verfasserIn] Lopez, Beatriz Zozio, Thierry Carrivale, Marcela Diaz, Chyntia Russomando, Graciela de Romero, Nilda J Jara, Juan C Barrera, Lucia Rastogi, Nalin Ritacco, Viviana

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2007

Schlagwörter:	Tuberculosis Restriction Fragment Length Polymorphism Restriction Fragment Length Polymorphism Analysis South American Country Restriction Fragment Length Polymorphism Pattern

Anmerkung:	© Candia et al; licensee BioMed Central Ltd. 2007

Übergeordnetes Werk:	Enthalten in: BMC microbiology - London : BioMed Central, 2001, 7(2007), 1 vom: 08. Aug.
Übergeordnetes Werk:	volume:7 ; year:2007 ; number:1 ; day:08 ; month:08

Links:	Volltext

DOI / URN:	10.1186/1471-2180-7-75

Katalog-ID:	SPR027177580

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245	1	0	\|a First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
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520			\|a Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe.
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allfields	10.1186/1471-2180-7-75 doi (DE-627)SPR027177580 (SPR)1471-2180-7-75-e DE-627 ger DE-627 rakwb eng Candia, Norma verfasserin aut First insight into Mycobacterium tuberculosis genetic diversity in Paraguay 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Candia et al; licensee BioMed Central Ltd. 2007 Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. Tuberculosis (dpeaa)DE-He213 Restriction Fragment Length Polymorphism (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Analysis (dpeaa)DE-He213 South American Country (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Pattern (dpeaa)DE-He213 Lopez, Beatriz aut Zozio, Thierry aut Carrivale, Marcela aut Diaz, Chyntia aut Russomando, Graciela aut de Romero, Nilda J aut Jara, Juan C aut Barrera, Lucia aut Rastogi, Nalin aut Ritacco, Viviana aut Enthalten in BMC microbiology London : BioMed Central, 2001 7(2007), 1 vom: 08. Aug. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:7 year:2007 number:1 day:08 month:08 https://dx.doi.org/10.1186/1471-2180-7-75 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 08 08
spelling	10.1186/1471-2180-7-75 doi (DE-627)SPR027177580 (SPR)1471-2180-7-75-e DE-627 ger DE-627 rakwb eng Candia, Norma verfasserin aut First insight into Mycobacterium tuberculosis genetic diversity in Paraguay 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Candia et al; licensee BioMed Central Ltd. 2007 Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. Tuberculosis (dpeaa)DE-He213 Restriction Fragment Length Polymorphism (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Analysis (dpeaa)DE-He213 South American Country (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Pattern (dpeaa)DE-He213 Lopez, Beatriz aut Zozio, Thierry aut Carrivale, Marcela aut Diaz, Chyntia aut Russomando, Graciela aut de Romero, Nilda J aut Jara, Juan C aut Barrera, Lucia aut Rastogi, Nalin aut Ritacco, Viviana aut Enthalten in BMC microbiology London : BioMed Central, 2001 7(2007), 1 vom: 08. Aug. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:7 year:2007 number:1 day:08 month:08 https://dx.doi.org/10.1186/1471-2180-7-75 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 08 08
allfields_unstemmed	10.1186/1471-2180-7-75 doi (DE-627)SPR027177580 (SPR)1471-2180-7-75-e DE-627 ger DE-627 rakwb eng Candia, Norma verfasserin aut First insight into Mycobacterium tuberculosis genetic diversity in Paraguay 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Candia et al; licensee BioMed Central Ltd. 2007 Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. Tuberculosis (dpeaa)DE-He213 Restriction Fragment Length Polymorphism (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Analysis (dpeaa)DE-He213 South American Country (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Pattern (dpeaa)DE-He213 Lopez, Beatriz aut Zozio, Thierry aut Carrivale, Marcela aut Diaz, Chyntia aut Russomando, Graciela aut de Romero, Nilda J aut Jara, Juan C aut Barrera, Lucia aut Rastogi, Nalin aut Ritacco, Viviana aut Enthalten in BMC microbiology London : BioMed Central, 2001 7(2007), 1 vom: 08. Aug. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:7 year:2007 number:1 day:08 month:08 https://dx.doi.org/10.1186/1471-2180-7-75 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 08 08
allfieldsGer	10.1186/1471-2180-7-75 doi (DE-627)SPR027177580 (SPR)1471-2180-7-75-e DE-627 ger DE-627 rakwb eng Candia, Norma verfasserin aut First insight into Mycobacterium tuberculosis genetic diversity in Paraguay 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Candia et al; licensee BioMed Central Ltd. 2007 Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. Tuberculosis (dpeaa)DE-He213 Restriction Fragment Length Polymorphism (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Analysis (dpeaa)DE-He213 South American Country (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Pattern (dpeaa)DE-He213 Lopez, Beatriz aut Zozio, Thierry aut Carrivale, Marcela aut Diaz, Chyntia aut Russomando, Graciela aut de Romero, Nilda J aut Jara, Juan C aut Barrera, Lucia aut Rastogi, Nalin aut Ritacco, Viviana aut Enthalten in BMC microbiology London : BioMed Central, 2001 7(2007), 1 vom: 08. Aug. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:7 year:2007 number:1 day:08 month:08 https://dx.doi.org/10.1186/1471-2180-7-75 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 08 08
allfieldsSound	10.1186/1471-2180-7-75 doi (DE-627)SPR027177580 (SPR)1471-2180-7-75-e DE-627 ger DE-627 rakwb eng Candia, Norma verfasserin aut First insight into Mycobacterium tuberculosis genetic diversity in Paraguay 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Candia et al; licensee BioMed Central Ltd. 2007 Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. Tuberculosis (dpeaa)DE-He213 Restriction Fragment Length Polymorphism (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Analysis (dpeaa)DE-He213 South American Country (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Pattern (dpeaa)DE-He213 Lopez, Beatriz aut Zozio, Thierry aut Carrivale, Marcela aut Diaz, Chyntia aut Russomando, Graciela aut de Romero, Nilda J aut Jara, Juan C aut Barrera, Lucia aut Rastogi, Nalin aut Ritacco, Viviana aut Enthalten in BMC microbiology London : BioMed Central, 2001 7(2007), 1 vom: 08. Aug. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:7 year:2007 number:1 day:08 month:08 https://dx.doi.org/10.1186/1471-2180-7-75 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 08 08
language	English
source	Enthalten in BMC microbiology 7(2007), 1 vom: 08. Aug. volume:7 year:2007 number:1 day:08 month:08
sourceStr	Enthalten in BMC microbiology 7(2007), 1 vom: 08. Aug. volume:7 year:2007 number:1 day:08 month:08
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Tuberculosis Restriction Fragment Length Polymorphism Restriction Fragment Length Polymorphism Analysis South American Country Restriction Fragment Length Polymorphism Pattern
isfreeaccess_bool	true
container_title	BMC microbiology
authorswithroles_txt_mv	Candia, Norma @@aut@@ Lopez, Beatriz @@aut@@ Zozio, Thierry @@aut@@ Carrivale, Marcela @@aut@@ Diaz, Chyntia @@aut@@ Russomando, Graciela @@aut@@ de Romero, Nilda J @@aut@@ Jara, Juan C @@aut@@ Barrera, Lucia @@aut@@ Rastogi, Nalin @@aut@@ Ritacco, Viviana @@aut@@
publishDateDaySort_date	2007-08-08T00:00:00Z
hierarchy_top_id	326644997
id	SPR027177580
language_de	englisch
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Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. 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author	Candia, Norma
spellingShingle	Candia, Norma misc Tuberculosis misc Restriction Fragment Length Polymorphism misc Restriction Fragment Length Polymorphism Analysis misc South American Country misc Restriction Fragment Length Polymorphism Pattern First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
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topic_title	First insight into Mycobacterium tuberculosis genetic diversity in Paraguay Tuberculosis (dpeaa)DE-He213 Restriction Fragment Length Polymorphism (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Analysis (dpeaa)DE-He213 South American Country (dpeaa)DE-He213 Restriction Fragment Length Polymorphism Pattern (dpeaa)DE-He213
topic	misc Tuberculosis misc Restriction Fragment Length Polymorphism misc Restriction Fragment Length Polymorphism Analysis misc South American Country misc Restriction Fragment Length Polymorphism Pattern
topic_unstemmed	misc Tuberculosis misc Restriction Fragment Length Polymorphism misc Restriction Fragment Length Polymorphism Analysis misc South American Country misc Restriction Fragment Length Polymorphism Pattern
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title	First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
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title_full	First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
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author_browse	Candia, Norma Lopez, Beatriz Zozio, Thierry Carrivale, Marcela Diaz, Chyntia Russomando, Graciela de Romero, Nilda J Jara, Juan C Barrera, Lucia Rastogi, Nalin Ritacco, Viviana
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title_sort	first insight into mycobacterium tuberculosis genetic diversity in paraguay
title_auth	First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
abstract	Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. © Candia et al; licensee BioMed Central Ltd. 2007
abstractGer	Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. © Candia et al; licensee BioMed Central Ltd. 2007
abstract_unstemmed	Background We present a picture of the biodiversity of Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with a tuberculosis notification rate of 38/100,000. Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. Conclusion Lineages currently thriving in Paraguay may reflect local host-pathogen adaptation of strains introduced during past migrations from Europe. © Candia et al; licensee BioMed Central Ltd. 2007
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title_short	First insight into Mycobacterium tuberculosis genetic diversity in Paraguay
url	https://dx.doi.org/10.1186/1471-2180-7-75
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author2	Lopez, Beatriz Zozio, Thierry Carrivale, Marcela Diaz, Chyntia Russomando, Graciela de Romero, Nilda J Jara, Juan C Barrera, Lucia Rastogi, Nalin Ritacco, Viviana
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Results A total of 220 strains collected throughout the country in 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns of 173 strains matched 51 shared international types (SITs) already present in an updated version of SpolDB4, the global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to the database 13 new SITs and 15 orphan spoligopatterns. Frequencies of major M. tuberculosis spoligotype lineages in our sample were as follows: Latin-American & Mediterranean (LAM) 52.3%, Haarlem 18.2%, S clade 9.5%, T superfamily 8.6%, X clade 0.9% and Beijing clade 0.5%. Concordant clustering by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping identified transmission in specific settings such as the Tacumbu jail in Asuncion and aboriginal communities in the Chaco. LAM genotypes were ubiquitous and predominated among both RFLP clusters and new patterns, suggesting ongoing transmission and adaptative evolution in Paraguay. We describe a new and successfully evolving clone of the Haarlem 3 sub-lineage, SIT2643, which is thus far restricted to Paraguay. We confirmed its clonality by RFLP and mycobacterial interspersed repetitive unit (MIRU) typing; we named it "Tacumbu" after the jail where it was found to be spreading. One-fifth of the spoligopatterns in our study are rarely or never seen outside Paraguay and one-tenth do not fit within any of the major phylogenetic clades in SpolDB4. 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First insight into Mycobacterium tuberculosis genetic diversity in Paraguay

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