PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha

Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in t...
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Autor*in:	Wahl, Andreas [verfasserIn] Schuth, Nora Pfeiffer, Daniel Nussberger, Stephan Jendrossek, Dieter

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	Poly(3-hydroxybutyrate) (PHB) Polyhydroxyalkanoate (PHA) PHB granule formation Storage metabolism PhaM Biodegradable polymer

Anmerkung:	© Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC microbiology - London : BioMed Central, 2001, 12(2012), 1 vom: 16. Nov.
Übergeordnetes Werk:	volume:12 ; year:2012 ; number:1 ; day:16 ; month:11

Links:	Volltext

DOI / URN:	10.1186/1471-2180-12-262

Katalog-ID:	SPR027192393

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520			\|a Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5.
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700	1		\|a Schuth, Nora \|4 aut
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700	1		\|a Nussberger, Stephan \|4 aut
700	1		\|a Jendrossek, Dieter \|4 aut
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allfields	10.1186/1471-2180-12-262 doi (DE-627)SPR027192393 (SPR)1471-2180-12-262-e DE-627 ger DE-627 rakwb eng Wahl, Andreas verfasserin aut PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. Poly(3-hydroxybutyrate) (PHB) (dpeaa)DE-He213 Polyhydroxyalkanoate (PHA) (dpeaa)DE-He213 PHB granule formation (dpeaa)DE-He213 Storage metabolism (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Biodegradable polymer (dpeaa)DE-He213 Schuth, Nora aut Pfeiffer, Daniel aut Nussberger, Stephan aut Jendrossek, Dieter aut Enthalten in BMC microbiology London : BioMed Central, 2001 12(2012), 1 vom: 16. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:12 year:2012 number:1 day:16 month:11 https://dx.doi.org/10.1186/1471-2180-12-262 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1 16 11
spelling	10.1186/1471-2180-12-262 doi (DE-627)SPR027192393 (SPR)1471-2180-12-262-e DE-627 ger DE-627 rakwb eng Wahl, Andreas verfasserin aut PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. Poly(3-hydroxybutyrate) (PHB) (dpeaa)DE-He213 Polyhydroxyalkanoate (PHA) (dpeaa)DE-He213 PHB granule formation (dpeaa)DE-He213 Storage metabolism (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Biodegradable polymer (dpeaa)DE-He213 Schuth, Nora aut Pfeiffer, Daniel aut Nussberger, Stephan aut Jendrossek, Dieter aut Enthalten in BMC microbiology London : BioMed Central, 2001 12(2012), 1 vom: 16. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:12 year:2012 number:1 day:16 month:11 https://dx.doi.org/10.1186/1471-2180-12-262 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1 16 11
allfields_unstemmed	10.1186/1471-2180-12-262 doi (DE-627)SPR027192393 (SPR)1471-2180-12-262-e DE-627 ger DE-627 rakwb eng Wahl, Andreas verfasserin aut PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. Poly(3-hydroxybutyrate) (PHB) (dpeaa)DE-He213 Polyhydroxyalkanoate (PHA) (dpeaa)DE-He213 PHB granule formation (dpeaa)DE-He213 Storage metabolism (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Biodegradable polymer (dpeaa)DE-He213 Schuth, Nora aut Pfeiffer, Daniel aut Nussberger, Stephan aut Jendrossek, Dieter aut Enthalten in BMC microbiology London : BioMed Central, 2001 12(2012), 1 vom: 16. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:12 year:2012 number:1 day:16 month:11 https://dx.doi.org/10.1186/1471-2180-12-262 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1 16 11
allfieldsGer	10.1186/1471-2180-12-262 doi (DE-627)SPR027192393 (SPR)1471-2180-12-262-e DE-627 ger DE-627 rakwb eng Wahl, Andreas verfasserin aut PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. Poly(3-hydroxybutyrate) (PHB) (dpeaa)DE-He213 Polyhydroxyalkanoate (PHA) (dpeaa)DE-He213 PHB granule formation (dpeaa)DE-He213 Storage metabolism (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Biodegradable polymer (dpeaa)DE-He213 Schuth, Nora aut Pfeiffer, Daniel aut Nussberger, Stephan aut Jendrossek, Dieter aut Enthalten in BMC microbiology London : BioMed Central, 2001 12(2012), 1 vom: 16. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:12 year:2012 number:1 day:16 month:11 https://dx.doi.org/10.1186/1471-2180-12-262 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1 16 11
allfieldsSound	10.1186/1471-2180-12-262 doi (DE-627)SPR027192393 (SPR)1471-2180-12-262-e DE-627 ger DE-627 rakwb eng Wahl, Andreas verfasserin aut PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. Poly(3-hydroxybutyrate) (PHB) (dpeaa)DE-He213 Polyhydroxyalkanoate (PHA) (dpeaa)DE-He213 PHB granule formation (dpeaa)DE-He213 Storage metabolism (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Biodegradable polymer (dpeaa)DE-He213 Schuth, Nora aut Pfeiffer, Daniel aut Nussberger, Stephan aut Jendrossek, Dieter aut Enthalten in BMC microbiology London : BioMed Central, 2001 12(2012), 1 vom: 16. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:12 year:2012 number:1 day:16 month:11 https://dx.doi.org/10.1186/1471-2180-12-262 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1 16 11
language	English
source	Enthalten in BMC microbiology 12(2012), 1 vom: 16. Nov. volume:12 year:2012 number:1 day:16 month:11
sourceStr	Enthalten in BMC microbiology 12(2012), 1 vom: 16. Nov. volume:12 year:2012 number:1 day:16 month:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Poly(3-hydroxybutyrate) (PHB) Polyhydroxyalkanoate (PHA) PHB granule formation Storage metabolism PhaM Biodegradable polymer
isfreeaccess_bool	false
container_title	BMC microbiology
authorswithroles_txt_mv	Wahl, Andreas @@aut@@ Schuth, Nora @@aut@@ Pfeiffer, Daniel @@aut@@ Nussberger, Stephan @@aut@@ Jendrossek, Dieter @@aut@@
publishDateDaySort_date	2012-11-16T00:00:00Z
hierarchy_top_id	326644997
id	SPR027192393
language_de	englisch
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Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. 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author	Wahl, Andreas
spellingShingle	Wahl, Andreas misc Poly(3-hydroxybutyrate) (PHB) misc Polyhydroxyalkanoate (PHA) misc PHB granule formation misc Storage metabolism misc PhaM misc Biodegradable polymer PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha
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topic_title	PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha Poly(3-hydroxybutyrate) (PHB) (dpeaa)DE-He213 Polyhydroxyalkanoate (PHA) (dpeaa)DE-He213 PHB granule formation (dpeaa)DE-He213 Storage metabolism (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Biodegradable polymer (dpeaa)DE-He213
topic	misc Poly(3-hydroxybutyrate) (PHB) misc Polyhydroxyalkanoate (PHA) misc PHB granule formation misc Storage metabolism misc PhaM misc Biodegradable polymer
topic_unstemmed	misc Poly(3-hydroxybutyrate) (PHB) misc Polyhydroxyalkanoate (PHA) misc PHB granule formation misc Storage metabolism misc PhaM misc Biodegradable polymer
topic_browse	misc Poly(3-hydroxybutyrate) (PHB) misc Polyhydroxyalkanoate (PHA) misc PHB granule formation misc Storage metabolism misc PhaM misc Biodegradable polymer
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title	PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha
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title_full	PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha
author_sort	Wahl, Andreas
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author_browse	Wahl, Andreas Schuth, Nora Pfeiffer, Daniel Nussberger, Stephan Jendrossek, Dieter
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author-letter	Wahl, Andreas
doi_str_mv	10.1186/1471-2180-12-262
title_sort	phb granules are attached to the nucleoid via pham in ralstonia eutropha
title_auth	PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha
abstract	Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles. Conclusion Subcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5. © Wahl et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha
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PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha

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