Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthes...
Ausführliche Beschreibung
Autor*in: |
Kasza, Aneta [verfasserIn] |
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E-Artikel |
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Englisch |
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2010 |
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Anmerkung: |
© Kasza et al; licensee BioMed Central Ltd. 2010 |
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Übergeordnetes Werk: |
Enthalten in: BMC molecular biology - London : BioMed Central, 2000, 11(2010), 1 vom: 06. Feb. |
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Übergeordnetes Werk: |
volume:11 ; year:2010 ; number:1 ; day:06 ; month:02 |
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DOI / URN: |
10.1186/1471-2199-11-14 |
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Katalog-ID: |
SPR02721849X |
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245 | 1 | 0 | |a Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression |
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520 | |a Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. | ||
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10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02 |
spelling |
10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02 |
allfields_unstemmed |
10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02 |
allfieldsGer |
10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02 |
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10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02 |
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transcription factors elk-1 and srf are engaged in il1-dependent regulation of zc3h12a expression |
title_auth |
Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression |
abstract |
Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. © Kasza et al; licensee BioMed Central Ltd. 2010 |
abstractGer |
Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. © Kasza et al; licensee BioMed Central Ltd. 2010 |
abstract_unstemmed |
Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. © Kasza et al; licensee BioMed Central Ltd. 2010 |
collection_details |
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container_issue |
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title_short |
Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression |
url |
https://dx.doi.org/10.1186/1471-2199-11-14 |
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Wyrzykowska, Paulina Horwacik, Irena Tymoszuk, Piotr Mizgalska, Danuta Palmer, Karren Rokita, Hanna Sharrocks, Andrew D Jura, Jolanta |
author2Str |
Wyrzykowska, Paulina Horwacik, Irena Tymoszuk, Piotr Mizgalska, Danuta Palmer, Karren Rokita, Hanna Sharrocks, Andrew D Jura, Jolanta |
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up_date |
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