Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression

Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthes...
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Autor*in:	Kasza, Aneta [verfasserIn] Wyrzykowska, Paulina Horwacik, Irena Tymoszuk, Piotr Mizgalska, Danuta Palmer, Karren Rokita, Hanna Sharrocks, Andrew D Jura, Jolanta

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	HepG2 Cell Serum Response Factor Mock Cell CCCH Zinc Finger Serum Response Factor Target

Anmerkung:	© Kasza et al; licensee BioMed Central Ltd. 2010

Übergeordnetes Werk:	Enthalten in: BMC molecular biology - London : BioMed Central, 2000, 11(2010), 1 vom: 06. Feb.
Übergeordnetes Werk:	volume:11 ; year:2010 ; number:1 ; day:06 ; month:02

Links:	Volltext

DOI / URN:	10.1186/1471-2199-11-14

Katalog-ID:	SPR02721849X

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100	1		\|a Kasza, Aneta \|e verfasserin \|4 aut
245	1	0	\|a Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
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520			\|a Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation.
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Indexfelder

author_variant	a k ak p w pw i h ih p t pt d m dm k p kp h r hr a d s ad ads j j jj
matchkey_str	article:14712199:2010----::rncitofcosl1nsfreggdnldpneteua
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publishDate	2010
allfields	10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02
spelling	10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02
allfields_unstemmed	10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02
allfieldsGer	10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02
allfieldsSound	10.1186/1471-2199-11-14 doi (DE-627)SPR02721849X (SPR)1471-2199-11-14-e DE-627 ger DE-627 rakwb eng Kasza, Aneta verfasserin aut Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Kasza et al; licensee BioMed Central Ltd. 2010 Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213 Wyrzykowska, Paulina aut Horwacik, Irena aut Tymoszuk, Piotr aut Mizgalska, Danuta aut Palmer, Karren aut Rokita, Hanna aut Sharrocks, Andrew D aut Jura, Jolanta aut Enthalten in BMC molecular biology London : BioMed Central, 2000 11(2010), 1 vom: 06. Feb. (DE-627)326645004 (DE-600)2041506-0 1471-2199 nnns volume:11 year:2010 number:1 day:06 month:02 https://dx.doi.org/10.1186/1471-2199-11-14 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1 06 02
language	English
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author	Kasza, Aneta
spellingShingle	Kasza, Aneta misc HepG2 Cell misc Serum Response Factor misc Mock Cell misc CCCH Zinc Finger misc Serum Response Factor Target Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
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topic_title	Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression HepG2 Cell (dpeaa)DE-He213 Serum Response Factor (dpeaa)DE-He213 Mock Cell (dpeaa)DE-He213 CCCH Zinc Finger (dpeaa)DE-He213 Serum Response Factor Target (dpeaa)DE-He213
topic	misc HepG2 Cell misc Serum Response Factor misc Mock Cell misc CCCH Zinc Finger misc Serum Response Factor Target
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title	Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
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title_full	Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
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author_browse	Kasza, Aneta Wyrzykowska, Paulina Horwacik, Irena Tymoszuk, Piotr Mizgalska, Danuta Palmer, Karren Rokita, Hanna Sharrocks, Andrew D Jura, Jolanta
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title_sort	transcription factors elk-1 and srf are engaged in il1-dependent regulation of zc3h12a expression
title_auth	Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
abstract	Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. © Kasza et al; licensee BioMed Central Ltd. 2010
abstractGer	Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. © Kasza et al; licensee BioMed Central Ltd. 2010
abstract_unstemmed	Background MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Results Here we report that the proinflammatory cytokine IL-1β rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1β. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter. Conclusions We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1β stimulation. © Kasza et al; licensee BioMed Central Ltd. 2010
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container_issue	1
title_short	Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression
url	https://dx.doi.org/10.1186/1471-2199-11-14
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author2	Wyrzykowska, Paulina Horwacik, Irena Tymoszuk, Piotr Mizgalska, Danuta Palmer, Karren Rokita, Hanna Sharrocks, Andrew D Jura, Jolanta
author2Str	Wyrzykowska, Paulina Horwacik, Irena Tymoszuk, Piotr Mizgalska, Danuta Palmer, Karren Rokita, Hanna Sharrocks, Andrew D Jura, Jolanta
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doi_str	10.1186/1471-2199-11-14
up_date	2024-07-04T00:56:27.022Z
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