Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride

Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and...
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Autor*in:	Navazio, Lorella [verfasserIn] Baldan, Barbara Moscatiello, Roberto Zuppini, Anna Woo, Sheridan L Mariani, Paola Lorito, Matteo

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2007

Schlagwörter:	Culture Filtrate Biocontrol Agent Induce Systemic Resistance Trichoderma Strain Soybean Cell

Anmerkung:	© Navazio et al; licensee BioMed Central Ltd. 2007

Übergeordnetes Werk:	Enthalten in: BMC plant biology - London : BioMed Central, 2001, 7(2007), 1 vom: 30. Juli
Übergeordnetes Werk:	volume:7 ; year:2007 ; number:1 ; day:30 ; month:07

Links:	Volltext

DOI / URN:	10.1186/1471-2229-7-41

Katalog-ID:	SPR02727988X

Internformat


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520			\|a Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses.
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allfields	10.1186/1471-2229-7-41 doi (DE-627)SPR02727988X (SPR)1471-2229-7-41-e DE-627 ger DE-627 rakwb eng Navazio, Lorella verfasserin aut Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Navazio et al; licensee BioMed Central Ltd. 2007 Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. Culture Filtrate (dpeaa)DE-He213 Biocontrol Agent (dpeaa)DE-He213 Induce Systemic Resistance (dpeaa)DE-He213 Trichoderma Strain (dpeaa)DE-He213 Soybean Cell (dpeaa)DE-He213 Baldan, Barbara aut Moscatiello, Roberto aut Zuppini, Anna aut Woo, Sheridan L aut Mariani, Paola aut Lorito, Matteo aut Enthalten in BMC plant biology London : BioMed Central, 2001 7(2007), 1 vom: 30. Juli (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:7 year:2007 number:1 day:30 month:07 https://dx.doi.org/10.1186/1471-2229-7-41 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 30 07
spelling	10.1186/1471-2229-7-41 doi (DE-627)SPR02727988X (SPR)1471-2229-7-41-e DE-627 ger DE-627 rakwb eng Navazio, Lorella verfasserin aut Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Navazio et al; licensee BioMed Central Ltd. 2007 Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. Culture Filtrate (dpeaa)DE-He213 Biocontrol Agent (dpeaa)DE-He213 Induce Systemic Resistance (dpeaa)DE-He213 Trichoderma Strain (dpeaa)DE-He213 Soybean Cell (dpeaa)DE-He213 Baldan, Barbara aut Moscatiello, Roberto aut Zuppini, Anna aut Woo, Sheridan L aut Mariani, Paola aut Lorito, Matteo aut Enthalten in BMC plant biology London : BioMed Central, 2001 7(2007), 1 vom: 30. Juli (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:7 year:2007 number:1 day:30 month:07 https://dx.doi.org/10.1186/1471-2229-7-41 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 30 07
allfields_unstemmed	10.1186/1471-2229-7-41 doi (DE-627)SPR02727988X (SPR)1471-2229-7-41-e DE-627 ger DE-627 rakwb eng Navazio, Lorella verfasserin aut Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Navazio et al; licensee BioMed Central Ltd. 2007 Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. Culture Filtrate (dpeaa)DE-He213 Biocontrol Agent (dpeaa)DE-He213 Induce Systemic Resistance (dpeaa)DE-He213 Trichoderma Strain (dpeaa)DE-He213 Soybean Cell (dpeaa)DE-He213 Baldan, Barbara aut Moscatiello, Roberto aut Zuppini, Anna aut Woo, Sheridan L aut Mariani, Paola aut Lorito, Matteo aut Enthalten in BMC plant biology London : BioMed Central, 2001 7(2007), 1 vom: 30. Juli (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:7 year:2007 number:1 day:30 month:07 https://dx.doi.org/10.1186/1471-2229-7-41 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 30 07
allfieldsGer	10.1186/1471-2229-7-41 doi (DE-627)SPR02727988X (SPR)1471-2229-7-41-e DE-627 ger DE-627 rakwb eng Navazio, Lorella verfasserin aut Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Navazio et al; licensee BioMed Central Ltd. 2007 Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. Culture Filtrate (dpeaa)DE-He213 Biocontrol Agent (dpeaa)DE-He213 Induce Systemic Resistance (dpeaa)DE-He213 Trichoderma Strain (dpeaa)DE-He213 Soybean Cell (dpeaa)DE-He213 Baldan, Barbara aut Moscatiello, Roberto aut Zuppini, Anna aut Woo, Sheridan L aut Mariani, Paola aut Lorito, Matteo aut Enthalten in BMC plant biology London : BioMed Central, 2001 7(2007), 1 vom: 30. Juli (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:7 year:2007 number:1 day:30 month:07 https://dx.doi.org/10.1186/1471-2229-7-41 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 30 07
allfieldsSound	10.1186/1471-2229-7-41 doi (DE-627)SPR02727988X (SPR)1471-2229-7-41-e DE-627 ger DE-627 rakwb eng Navazio, Lorella verfasserin aut Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Navazio et al; licensee BioMed Central Ltd. 2007 Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. Culture Filtrate (dpeaa)DE-He213 Biocontrol Agent (dpeaa)DE-He213 Induce Systemic Resistance (dpeaa)DE-He213 Trichoderma Strain (dpeaa)DE-He213 Soybean Cell (dpeaa)DE-He213 Baldan, Barbara aut Moscatiello, Roberto aut Zuppini, Anna aut Woo, Sheridan L aut Mariani, Paola aut Lorito, Matteo aut Enthalten in BMC plant biology London : BioMed Central, 2001 7(2007), 1 vom: 30. Juli (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:7 year:2007 number:1 day:30 month:07 https://dx.doi.org/10.1186/1471-2229-7-41 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2007 1 30 07
language	English
source	Enthalten in BMC plant biology 7(2007), 1 vom: 30. Juli volume:7 year:2007 number:1 day:30 month:07
sourceStr	Enthalten in BMC plant biology 7(2007), 1 vom: 30. Juli volume:7 year:2007 number:1 day:30 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Culture Filtrate Biocontrol Agent Induce Systemic Resistance Trichoderma Strain Soybean Cell
isfreeaccess_bool	true
container_title	BMC plant biology
authorswithroles_txt_mv	Navazio, Lorella @@aut@@ Baldan, Barbara @@aut@@ Moscatiello, Roberto @@aut@@ Zuppini, Anna @@aut@@ Woo, Sheridan L @@aut@@ Mariani, Paola @@aut@@ Lorito, Matteo @@aut@@
publishDateDaySort_date	2007-07-30T00:00:00Z
hierarchy_top_id	335489060
id	SPR02727988X
language_de	englisch
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Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. 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author	Navazio, Lorella
spellingShingle	Navazio, Lorella misc Culture Filtrate misc Biocontrol Agent misc Induce Systemic Resistance misc Trichoderma Strain misc Soybean Cell Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride
authorStr	Navazio, Lorella
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topic_title	Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride Culture Filtrate (dpeaa)DE-He213 Biocontrol Agent (dpeaa)DE-He213 Induce Systemic Resistance (dpeaa)DE-He213 Trichoderma Strain (dpeaa)DE-He213 Soybean Cell (dpeaa)DE-He213
topic	misc Culture Filtrate misc Biocontrol Agent misc Induce Systemic Resistance misc Trichoderma Strain misc Soybean Cell
topic_unstemmed	misc Culture Filtrate misc Biocontrol Agent misc Induce Systemic Resistance misc Trichoderma Strain misc Soybean Cell
topic_browse	misc Culture Filtrate misc Biocontrol Agent misc Induce Systemic Resistance misc Trichoderma Strain misc Soybean Cell
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title	Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride
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title_full	Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride
author_sort	Navazio, Lorella
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author_browse	Navazio, Lorella Baldan, Barbara Moscatiello, Roberto Zuppini, Anna Woo, Sheridan L Mariani, Paola Lorito, Matteo
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doi_str_mv	10.1186/1471-2229-7-41
title_sort	calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus trichoderma atroviride
title_auth	Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride
abstract	Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. © Navazio et al; licensee BioMed Central Ltd. 2007
abstractGer	Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. © Navazio et al; licensee BioMed Central Ltd. 2007
abstract_unstemmed	Background Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. Results Here, we investigated the effects on plant cells of metabolite complexes secreted by Trichoderma atroviride wild type P1 and a deletion mutant of this strain on the level of cytosolic free $ Ca^{2+} $ and activation of defense responses. Trichoderma culture filtrates were obtained by growing the fungus alone or in direct antagonism with its fungal host, the necrotrophic pathogen Botrytis cinerea, and then separated in two fractions (>3 and <3 kDa). When applied to aequorin-expressing soybean (Glycine max L.) cell suspension cultures, Trichoderma and Botrytis metabolite mixtures were distinctively perceived and activated transient intracellular $ Ca^{2+} $ elevations with different kinetics, specific patterns of intracellular accumulation of reactive oxygen species and induction of cell death. Both $ Ca^{2+} $ signature and cellular effects were modified by the culture medium from the knock-out mutant of Trichoderma, defective for the production of the secreted 42 kDa endochitinase. Conclusion New insights are provided into the mechanism of interaction between Trichoderma and plants, indicating that secreted fungal molecules are sensed by plant cells through intracellular $ Ca^{2+} $ changes. Plant cells are able to discriminate signals originating in the single or two-fungal partner interaction and modulate defense responses. © Navazio et al; licensee BioMed Central Ltd. 2007
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title_short	Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride
url	https://dx.doi.org/10.1186/1471-2229-7-41
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Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride

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