RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines

Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we crea...
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Autor*in:	Arro, Jie [verfasserIn] Yang, Yingzhen Song, Guo-Qing Zhong, Gan-Yuan

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	CONSTANS DELLA Flowering Grapevine HOMEODOMAIN Perennial TFL1 Transgenic GA-insensitive

Anmerkung:	© The Author(s). 2019

Übergeordnetes Werk:	Enthalten in: BMC plant biology - London : BioMed Central, 2001, 19(2019), 1 vom: 18. Feb.
Übergeordnetes Werk:	volume:19 ; year:2019 ; number:1 ; day:18 ; month:02

Links:	Volltext

DOI / URN:	10.1186/s12870-019-1675-4

Katalog-ID:	SPR027309479

Internformat


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245	1	0	\|a RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines
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520			\|a Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling.
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publishDate	2019
allfields	10.1186/s12870-019-1675-4 doi (DE-627)SPR027309479 (SPR)s12870-019-1675-4-e DE-627 ger DE-627 rakwb eng Arro, Jie verfasserin aut RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. CONSTANS (dpeaa)DE-He213 DELLA (dpeaa)DE-He213 Flowering (dpeaa)DE-He213 Grapevine (dpeaa)DE-He213 HOMEODOMAIN (dpeaa)DE-He213 Perennial (dpeaa)DE-He213 TFL1 (dpeaa)DE-He213 Transgenic GA-insensitive (dpeaa)DE-He213 Yang, Yingzhen aut Song, Guo-Qing aut Zhong, Gan-Yuan aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 18. Feb. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:18 month:02 https://dx.doi.org/10.1186/s12870-019-1675-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 18 02
spelling	10.1186/s12870-019-1675-4 doi (DE-627)SPR027309479 (SPR)s12870-019-1675-4-e DE-627 ger DE-627 rakwb eng Arro, Jie verfasserin aut RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. CONSTANS (dpeaa)DE-He213 DELLA (dpeaa)DE-He213 Flowering (dpeaa)DE-He213 Grapevine (dpeaa)DE-He213 HOMEODOMAIN (dpeaa)DE-He213 Perennial (dpeaa)DE-He213 TFL1 (dpeaa)DE-He213 Transgenic GA-insensitive (dpeaa)DE-He213 Yang, Yingzhen aut Song, Guo-Qing aut Zhong, Gan-Yuan aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 18. Feb. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:18 month:02 https://dx.doi.org/10.1186/s12870-019-1675-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 18 02
allfields_unstemmed	10.1186/s12870-019-1675-4 doi (DE-627)SPR027309479 (SPR)s12870-019-1675-4-e DE-627 ger DE-627 rakwb eng Arro, Jie verfasserin aut RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. CONSTANS (dpeaa)DE-He213 DELLA (dpeaa)DE-He213 Flowering (dpeaa)DE-He213 Grapevine (dpeaa)DE-He213 HOMEODOMAIN (dpeaa)DE-He213 Perennial (dpeaa)DE-He213 TFL1 (dpeaa)DE-He213 Transgenic GA-insensitive (dpeaa)DE-He213 Yang, Yingzhen aut Song, Guo-Qing aut Zhong, Gan-Yuan aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 18. Feb. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:18 month:02 https://dx.doi.org/10.1186/s12870-019-1675-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 18 02
allfieldsGer	10.1186/s12870-019-1675-4 doi (DE-627)SPR027309479 (SPR)s12870-019-1675-4-e DE-627 ger DE-627 rakwb eng Arro, Jie verfasserin aut RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. CONSTANS (dpeaa)DE-He213 DELLA (dpeaa)DE-He213 Flowering (dpeaa)DE-He213 Grapevine (dpeaa)DE-He213 HOMEODOMAIN (dpeaa)DE-He213 Perennial (dpeaa)DE-He213 TFL1 (dpeaa)DE-He213 Transgenic GA-insensitive (dpeaa)DE-He213 Yang, Yingzhen aut Song, Guo-Qing aut Zhong, Gan-Yuan aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 18. Feb. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:18 month:02 https://dx.doi.org/10.1186/s12870-019-1675-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 18 02
allfieldsSound	10.1186/s12870-019-1675-4 doi (DE-627)SPR027309479 (SPR)s12870-019-1675-4-e DE-627 ger DE-627 rakwb eng Arro, Jie verfasserin aut RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. CONSTANS (dpeaa)DE-He213 DELLA (dpeaa)DE-He213 Flowering (dpeaa)DE-He213 Grapevine (dpeaa)DE-He213 HOMEODOMAIN (dpeaa)DE-He213 Perennial (dpeaa)DE-He213 TFL1 (dpeaa)DE-He213 Transgenic GA-insensitive (dpeaa)DE-He213 Yang, Yingzhen aut Song, Guo-Qing aut Zhong, Gan-Yuan aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 18. Feb. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:18 month:02 https://dx.doi.org/10.1186/s12870-019-1675-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 18 02
language	English
source	Enthalten in BMC plant biology 19(2019), 1 vom: 18. Feb. volume:19 year:2019 number:1 day:18 month:02
sourceStr	Enthalten in BMC plant biology 19(2019), 1 vom: 18. Feb. volume:19 year:2019 number:1 day:18 month:02
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CONSTANS DELLA Flowering Grapevine HOMEODOMAIN Perennial TFL1 Transgenic GA-insensitive
isfreeaccess_bool	true
container_title	BMC plant biology
authorswithroles_txt_mv	Arro, Jie @@aut@@ Yang, Yingzhen @@aut@@ Song, Guo-Qing @@aut@@ Zhong, Gan-Yuan @@aut@@
publishDateDaySort_date	2019-02-18T00:00:00Z
hierarchy_top_id	335489060
id	SPR027309479
language_de	englisch
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Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. 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author	Arro, Jie
spellingShingle	Arro, Jie misc CONSTANS misc DELLA misc Flowering misc Grapevine misc HOMEODOMAIN misc Perennial misc TFL1 misc Transgenic GA-insensitive RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines
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topic_title	RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines CONSTANS (dpeaa)DE-He213 DELLA (dpeaa)DE-He213 Flowering (dpeaa)DE-He213 Grapevine (dpeaa)DE-He213 HOMEODOMAIN (dpeaa)DE-He213 Perennial (dpeaa)DE-He213 TFL1 (dpeaa)DE-He213 Transgenic GA-insensitive (dpeaa)DE-He213
topic	misc CONSTANS misc DELLA misc Flowering misc Grapevine misc HOMEODOMAIN misc Perennial misc TFL1 misc Transgenic GA-insensitive
topic_unstemmed	misc CONSTANS misc DELLA misc Flowering misc Grapevine misc HOMEODOMAIN misc Perennial misc TFL1 misc Transgenic GA-insensitive
topic_browse	misc CONSTANS misc DELLA misc Flowering misc Grapevine misc HOMEODOMAIN misc Perennial misc TFL1 misc Transgenic GA-insensitive
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title	RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines
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title_full	RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines
author_sort	Arro, Jie
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author_browse	Arro, Jie Yang, Yingzhen Song, Guo-Qing Zhong, Gan-Yuan
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author-letter	Arro, Jie
doi_str_mv	10.1186/s12870-019-1675-4
title_sort	rna-seq reveals new della targets and regulation in transgenic ga-insensitive grapevines
title_auth	RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines
abstract	Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. © The Author(s). 2019
abstractGer	Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. © The Author(s). 2019
abstract_unstemmed	Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1. Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. © The Author(s). 2019
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title_short	RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines
url	https://dx.doi.org/10.1186/s12870-019-1675-4
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author2	Yang, Yingzhen Song, Guo-Qing Zhong, Gan-Yuan
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up_date	2024-07-04T01:18:02.526Z
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RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines

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