Improving salt tolerance in potato through overexpression of AtHKT1 gene

Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the...
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Autor*in:	Wang, Li [verfasserIn] Liu, Yuhui Li, Dan Feng, Shoujiang Yang, Jiangwei Zhang, Jingjing Zhang, Junlian Wang, Di Gan, Yantai

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	gene K /Na ratio Photosynthetic rate Stomatal conductance Transpiration rate

Anmerkung:	© The Author(s). 2019

Übergeordnetes Werk:	Enthalten in: BMC plant biology - London : BioMed Central, 2001, 19(2019), 1 vom: 16. Aug.
Übergeordnetes Werk:	volume:19 ; year:2019 ; number:1 ; day:16 ; month:08

Links:	Volltext

DOI / URN:	10.1186/s12870-019-1963-z

Katalog-ID:	SPR027312364

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520			\|a Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity.
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700	1		\|a Wang, Di \|4 aut
700	1		\|a Gan, Yantai \|0 (orcid)0000-0002-9074-0357 \|4 aut
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publishDate	2019
allfields	10.1186/s12870-019-1963-z doi (DE-627)SPR027312364 (SPR)s12870-019-1963-z-e DE-627 ger DE-627 rakwb eng Wang, Li verfasserin (orcid)0000-0002-6937-7813 aut Improving salt tolerance in potato through overexpression of AtHKT1 gene 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. gene (dpeaa)DE-He213 K (dpeaa)DE-He213 /Na (dpeaa)DE-He213 ratio (dpeaa)DE-He213 Photosynthetic rate (dpeaa)DE-He213 Stomatal conductance (dpeaa)DE-He213 Transpiration rate (dpeaa)DE-He213 Liu, Yuhui aut Li, Dan aut Feng, Shoujiang aut Yang, Jiangwei aut Zhang, Jingjing aut Zhang, Junlian (orcid)0000-0001-8093-5495 aut Wang, Di aut Gan, Yantai (orcid)0000-0002-9074-0357 aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 16. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:16 month:08 https://dx.doi.org/10.1186/s12870-019-1963-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 16 08
spelling	10.1186/s12870-019-1963-z doi (DE-627)SPR027312364 (SPR)s12870-019-1963-z-e DE-627 ger DE-627 rakwb eng Wang, Li verfasserin (orcid)0000-0002-6937-7813 aut Improving salt tolerance in potato through overexpression of AtHKT1 gene 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. gene (dpeaa)DE-He213 K (dpeaa)DE-He213 /Na (dpeaa)DE-He213 ratio (dpeaa)DE-He213 Photosynthetic rate (dpeaa)DE-He213 Stomatal conductance (dpeaa)DE-He213 Transpiration rate (dpeaa)DE-He213 Liu, Yuhui aut Li, Dan aut Feng, Shoujiang aut Yang, Jiangwei aut Zhang, Jingjing aut Zhang, Junlian (orcid)0000-0001-8093-5495 aut Wang, Di aut Gan, Yantai (orcid)0000-0002-9074-0357 aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 16. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:16 month:08 https://dx.doi.org/10.1186/s12870-019-1963-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 16 08
allfields_unstemmed	10.1186/s12870-019-1963-z doi (DE-627)SPR027312364 (SPR)s12870-019-1963-z-e DE-627 ger DE-627 rakwb eng Wang, Li verfasserin (orcid)0000-0002-6937-7813 aut Improving salt tolerance in potato through overexpression of AtHKT1 gene 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. gene (dpeaa)DE-He213 K (dpeaa)DE-He213 /Na (dpeaa)DE-He213 ratio (dpeaa)DE-He213 Photosynthetic rate (dpeaa)DE-He213 Stomatal conductance (dpeaa)DE-He213 Transpiration rate (dpeaa)DE-He213 Liu, Yuhui aut Li, Dan aut Feng, Shoujiang aut Yang, Jiangwei aut Zhang, Jingjing aut Zhang, Junlian (orcid)0000-0001-8093-5495 aut Wang, Di aut Gan, Yantai (orcid)0000-0002-9074-0357 aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 16. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:16 month:08 https://dx.doi.org/10.1186/s12870-019-1963-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 16 08
allfieldsGer	10.1186/s12870-019-1963-z doi (DE-627)SPR027312364 (SPR)s12870-019-1963-z-e DE-627 ger DE-627 rakwb eng Wang, Li verfasserin (orcid)0000-0002-6937-7813 aut Improving salt tolerance in potato through overexpression of AtHKT1 gene 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. gene (dpeaa)DE-He213 K (dpeaa)DE-He213 /Na (dpeaa)DE-He213 ratio (dpeaa)DE-He213 Photosynthetic rate (dpeaa)DE-He213 Stomatal conductance (dpeaa)DE-He213 Transpiration rate (dpeaa)DE-He213 Liu, Yuhui aut Li, Dan aut Feng, Shoujiang aut Yang, Jiangwei aut Zhang, Jingjing aut Zhang, Junlian (orcid)0000-0001-8093-5495 aut Wang, Di aut Gan, Yantai (orcid)0000-0002-9074-0357 aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 16. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:16 month:08 https://dx.doi.org/10.1186/s12870-019-1963-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 16 08
allfieldsSound	10.1186/s12870-019-1963-z doi (DE-627)SPR027312364 (SPR)s12870-019-1963-z-e DE-627 ger DE-627 rakwb eng Wang, Li verfasserin (orcid)0000-0002-6937-7813 aut Improving salt tolerance in potato through overexpression of AtHKT1 gene 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2019 Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. gene (dpeaa)DE-He213 K (dpeaa)DE-He213 /Na (dpeaa)DE-He213 ratio (dpeaa)DE-He213 Photosynthetic rate (dpeaa)DE-He213 Stomatal conductance (dpeaa)DE-He213 Transpiration rate (dpeaa)DE-He213 Liu, Yuhui aut Li, Dan aut Feng, Shoujiang aut Yang, Jiangwei aut Zhang, Jingjing aut Zhang, Junlian (orcid)0000-0001-8093-5495 aut Wang, Di aut Gan, Yantai (orcid)0000-0002-9074-0357 aut Enthalten in BMC plant biology London : BioMed Central, 2001 19(2019), 1 vom: 16. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:19 year:2019 number:1 day:16 month:08 https://dx.doi.org/10.1186/s12870-019-1963-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2019 1 16 08
language	English
source	Enthalten in BMC plant biology 19(2019), 1 vom: 16. Aug. volume:19 year:2019 number:1 day:16 month:08
sourceStr	Enthalten in BMC plant biology 19(2019), 1 vom: 16. Aug. volume:19 year:2019 number:1 day:16 month:08
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	gene K /Na ratio Photosynthetic rate Stomatal conductance Transpiration rate
isfreeaccess_bool	true
container_title	BMC plant biology
authorswithroles_txt_mv	Wang, Li @@aut@@ Liu, Yuhui @@aut@@ Li, Dan @@aut@@ Feng, Shoujiang @@aut@@ Yang, Jiangwei @@aut@@ Zhang, Jingjing @@aut@@ Zhang, Junlian @@aut@@ Wang, Di @@aut@@ Gan, Yantai @@aut@@
publishDateDaySort_date	2019-08-16T00:00:00Z
hierarchy_top_id	335489060
id	SPR027312364
language_de	englisch
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Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">gene</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">K</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">/Na</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ratio</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Photosynthetic rate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Stomatal conductance</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transpiration rate</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Yuhui</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Dan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Feng, Shoujiang</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Jiangwei</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Jingjing</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Junlian</subfield><subfield code="0">(orcid)0000-0001-8093-5495</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Di</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gan, Yantai</subfield><subfield code="0">(orcid)0000-0002-9074-0357</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">BMC plant biology</subfield><subfield code="d">London : BioMed Central, 2001</subfield><subfield code="g">19(2019), 1 vom: 16. 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author	Wang, Li
spellingShingle	Wang, Li misc gene misc K misc /Na misc ratio misc Photosynthetic rate misc Stomatal conductance misc Transpiration rate Improving salt tolerance in potato through overexpression of AtHKT1 gene
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topic_title	Improving salt tolerance in potato through overexpression of AtHKT1 gene gene (dpeaa)DE-He213 K (dpeaa)DE-He213 /Na (dpeaa)DE-He213 ratio (dpeaa)DE-He213 Photosynthetic rate (dpeaa)DE-He213 Stomatal conductance (dpeaa)DE-He213 Transpiration rate (dpeaa)DE-He213
topic	misc gene misc K misc /Na misc ratio misc Photosynthetic rate misc Stomatal conductance misc Transpiration rate
topic_unstemmed	misc gene misc K misc /Na misc ratio misc Photosynthetic rate misc Stomatal conductance misc Transpiration rate
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title	Improving salt tolerance in potato through overexpression of AtHKT1 gene
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title_full	Improving salt tolerance in potato through overexpression of AtHKT1 gene
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author_browse	Wang, Li Liu, Yuhui Li, Dan Feng, Shoujiang Yang, Jiangwei Zhang, Jingjing Zhang, Junlian Wang, Di Gan, Yantai
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title_sort	improving salt tolerance in potato through overexpression of athkt1 gene
title_auth	Improving salt tolerance in potato through overexpression of AtHKT1 gene
abstract	Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. © The Author(s). 2019
abstractGer	Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. © The Author(s). 2019
abstract_unstemmed	Background Survival of plants in response to salinity stress is typically related to $ Na^{+} $ toxicity, but little is known about how heterologous high-affinity potassium transporter (HKT) may help alleviate salt-induced damages in potato (Solanum tuberosum L.). Results In this study, we used the Arabidopsis thaliana high-affinity potassium transporter gene (AtHKT1) to enhance the capacity of potato plants to tolerate salinity stress by decreasing $ Na^{+} $ content and improving $ K^{+} $/$ Na^{+} $ ratio in plant leaves, while maintaining osmotic balance. Seven AtHKT1 transformed potato lines (namely T1, T2, T3, T5, T11, T13 and T15) were compared with non-transgenic control plant at molecule and whole-plant levels. The lines T3 and T13 had the highest AtHKT1 expression with the tolerance index (an quantitative assessment) being 6.8 times that of the control. At 30 days under 100 and 150 mmol $ L^{− 1} $ NaCl stress treatments, the T3 and T13 lines had least reductions in net photosynthetic rate, stomatal conductance and transpiration rate among the seven lines, leading to the increased water use efficiency and decreased yield loss. Conclusions We conclude that the constitutive overexpression of AtHKT1 reduces $ Na^{+} $ accumulation in potato leaves and promotes the $ K^{+} $/$ Na^{+} $ homeostasis that minimizes osmotic imbalance, maintains photosynthesis and stomatal conductance, and increases plant productivity. © The Author(s). 2019
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title_short	Improving salt tolerance in potato through overexpression of AtHKT1 gene
url	https://dx.doi.org/10.1186/s12870-019-1963-z
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author2	Liu, Yuhui Li, Dan Feng, Shoujiang Yang, Jiangwei Zhang, Jingjing Zhang, Junlian Wang, Di Gan, Yantai
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Improving salt tolerance in potato through overexpression of AtHKT1 gene

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