Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease

Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli ca...
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Autor*in:	Elliott, Timothy R. [verfasserIn] Rayment, Neil B. Hudspith, Barry N. Hands, Rebecca E. Taylor, Kirstin Parkes, Gareth C. Prescott, Natalie J. Petrovska, Liljana Hermon-Taylor, John Brostoff, Jonathan Boussioutas, Alex Mathew, Christopher G. Bustin, Stephen A. Sanderson, Jeremy D.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Crohn’s disease Macrophages

Anmerkung:	© Elliott et al. 2015

Übergeordnetes Werk:	Enthalten in: BMC gastroenterology - London : BioMed Central, 2001, 15(2015), 1 vom: 03. Juli
Übergeordnetes Werk:	volume:15 ; year:2015 ; number:1 ; day:03 ; month:07

Links:	Volltext

DOI / URN:	10.1186/s12876-015-0305-3

Katalog-ID:	SPR027416550

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520			\|a Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation.
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Indexfelder

author_variant	t r e tr tre n b r nb nbr b n h bn bnh r e h re reh k t kt g c p gc gcp n j p nj njp l p lp j h t jht j b jb a b ab c g m cg cgm s a b sa sab j d s jd jds
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publishDate	2015
allfields	10.1186/s12876-015-0305-3 doi (DE-627)SPR027416550 (SPR)s12876-015-0305-3-e DE-627 ger DE-627 rakwb eng Elliott, Timothy R. verfasserin aut Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Elliott et al. 2015 Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. Crohn’s disease (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Rayment, Neil B. aut Hudspith, Barry N. aut Hands, Rebecca E. aut Taylor, Kirstin aut Parkes, Gareth C. aut Prescott, Natalie J. aut Petrovska, Liljana aut Hermon-Taylor, John aut Brostoff, Jonathan aut Boussioutas, Alex aut Mathew, Christopher G. aut Bustin, Stephen A. aut Sanderson, Jeremy D. aut Enthalten in BMC gastroenterology London : BioMed Central, 2001 15(2015), 1 vom: 03. Juli (DE-627)326643702 (DE-600)2041351-8 1471-230X nnns volume:15 year:2015 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12876-015-0305-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 03 07
spelling	10.1186/s12876-015-0305-3 doi (DE-627)SPR027416550 (SPR)s12876-015-0305-3-e DE-627 ger DE-627 rakwb eng Elliott, Timothy R. verfasserin aut Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Elliott et al. 2015 Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. Crohn’s disease (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Rayment, Neil B. aut Hudspith, Barry N. aut Hands, Rebecca E. aut Taylor, Kirstin aut Parkes, Gareth C. aut Prescott, Natalie J. aut Petrovska, Liljana aut Hermon-Taylor, John aut Brostoff, Jonathan aut Boussioutas, Alex aut Mathew, Christopher G. aut Bustin, Stephen A. aut Sanderson, Jeremy D. aut Enthalten in BMC gastroenterology London : BioMed Central, 2001 15(2015), 1 vom: 03. Juli (DE-627)326643702 (DE-600)2041351-8 1471-230X nnns volume:15 year:2015 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12876-015-0305-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 03 07
allfields_unstemmed	10.1186/s12876-015-0305-3 doi (DE-627)SPR027416550 (SPR)s12876-015-0305-3-e DE-627 ger DE-627 rakwb eng Elliott, Timothy R. verfasserin aut Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Elliott et al. 2015 Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. Crohn’s disease (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Rayment, Neil B. aut Hudspith, Barry N. aut Hands, Rebecca E. aut Taylor, Kirstin aut Parkes, Gareth C. aut Prescott, Natalie J. aut Petrovska, Liljana aut Hermon-Taylor, John aut Brostoff, Jonathan aut Boussioutas, Alex aut Mathew, Christopher G. aut Bustin, Stephen A. aut Sanderson, Jeremy D. aut Enthalten in BMC gastroenterology London : BioMed Central, 2001 15(2015), 1 vom: 03. Juli (DE-627)326643702 (DE-600)2041351-8 1471-230X nnns volume:15 year:2015 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12876-015-0305-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 03 07
allfieldsGer	10.1186/s12876-015-0305-3 doi (DE-627)SPR027416550 (SPR)s12876-015-0305-3-e DE-627 ger DE-627 rakwb eng Elliott, Timothy R. verfasserin aut Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Elliott et al. 2015 Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. Crohn’s disease (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Rayment, Neil B. aut Hudspith, Barry N. aut Hands, Rebecca E. aut Taylor, Kirstin aut Parkes, Gareth C. aut Prescott, Natalie J. aut Petrovska, Liljana aut Hermon-Taylor, John aut Brostoff, Jonathan aut Boussioutas, Alex aut Mathew, Christopher G. aut Bustin, Stephen A. aut Sanderson, Jeremy D. aut Enthalten in BMC gastroenterology London : BioMed Central, 2001 15(2015), 1 vom: 03. Juli (DE-627)326643702 (DE-600)2041351-8 1471-230X nnns volume:15 year:2015 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12876-015-0305-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 03 07
allfieldsSound	10.1186/s12876-015-0305-3 doi (DE-627)SPR027416550 (SPR)s12876-015-0305-3-e DE-627 ger DE-627 rakwb eng Elliott, Timothy R. verfasserin aut Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Elliott et al. 2015 Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. Crohn’s disease (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Rayment, Neil B. aut Hudspith, Barry N. aut Hands, Rebecca E. aut Taylor, Kirstin aut Parkes, Gareth C. aut Prescott, Natalie J. aut Petrovska, Liljana aut Hermon-Taylor, John aut Brostoff, Jonathan aut Boussioutas, Alex aut Mathew, Christopher G. aut Bustin, Stephen A. aut Sanderson, Jeremy D. aut Enthalten in BMC gastroenterology London : BioMed Central, 2001 15(2015), 1 vom: 03. Juli (DE-627)326643702 (DE-600)2041351-8 1471-230X nnns volume:15 year:2015 number:1 day:03 month:07 https://dx.doi.org/10.1186/s12876-015-0305-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2015 1 03 07
language	English
source	Enthalten in BMC gastroenterology 15(2015), 1 vom: 03. Juli volume:15 year:2015 number:1 day:03 month:07
sourceStr	Enthalten in BMC gastroenterology 15(2015), 1 vom: 03. Juli volume:15 year:2015 number:1 day:03 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Crohn’s disease Macrophages
isfreeaccess_bool	true
container_title	BMC gastroenterology
authorswithroles_txt_mv	Elliott, Timothy R. @@aut@@ Rayment, Neil B. @@aut@@ Hudspith, Barry N. @@aut@@ Hands, Rebecca E. @@aut@@ Taylor, Kirstin @@aut@@ Parkes, Gareth C. @@aut@@ Prescott, Natalie J. @@aut@@ Petrovska, Liljana @@aut@@ Hermon-Taylor, John @@aut@@ Brostoff, Jonathan @@aut@@ Boussioutas, Alex @@aut@@ Mathew, Christopher G. @@aut@@ Bustin, Stephen A. @@aut@@ Sanderson, Jeremy D. @@aut@@
publishDateDaySort_date	2015-07-03T00:00:00Z
hierarchy_top_id	326643702
id	SPR027416550
language_de	englisch
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We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. 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author	Elliott, Timothy R.
spellingShingle	Elliott, Timothy R. misc Crohn’s disease misc Macrophages Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease
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topic_title	Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease Crohn’s disease (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213
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title	Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease
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title_full	Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease
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author_browse	Elliott, Timothy R. Rayment, Neil B. Hudspith, Barry N. Hands, Rebecca E. Taylor, Kirstin Parkes, Gareth C. Prescott, Natalie J. Petrovska, Liljana Hermon-Taylor, John Brostoff, Jonathan Boussioutas, Alex Mathew, Christopher G. Bustin, Stephen A. Sanderson, Jeremy D.
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author-letter	Elliott, Timothy R.
doi_str_mv	10.1186/s12876-015-0305-3
title_sort	lamina propria macrophage phenotypes in relation to escherichia coli in crohn’s disease
title_auth	Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease
abstract	Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. © Elliott et al. 2015
abstractGer	Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. © Elliott et al. 2015
abstract_unstemmed	Background Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation. © Elliott et al. 2015
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title_short	Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease
url	https://dx.doi.org/10.1186/s12876-015-0305-3
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author2	Rayment, Neil B. Hudspith, Barry N. Hands, Rebecca E. Taylor, Kirstin Parkes, Gareth C. Prescott, Natalie J. Petrovska, Liljana Hermon-Taylor, John Brostoff, Jonathan Boussioutas, Alex Mathew, Christopher G. Bustin, Stephen A. Sanderson, Jeremy D.
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Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease

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