Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples
Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Janecka, Jan E. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media Dordrecht 2013 |
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| Übergeordnetes Werk: |
Enthalten in: Conservation genetics resources - Dordrecht : Springer Netherlands, 2009, 6(2013), 2 vom: 06. Dez., Seite 369-373 |
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| Übergeordnetes Werk: |
volume:6 ; year:2013 ; number:2 ; day:06 ; month:12 ; pages:369-373 |
| Links: |
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| DOI / URN: |
10.1007/s12686-013-0096-1 |
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| Katalog-ID: |
SPR027439194 |
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| 100 | 1 | |a Janecka, Jan E. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
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| 520 | |a Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. | ||
| 650 | 4 | |a Microsatellites |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Snow leopard |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Noninvasive genetics |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Individual identification |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Jackson, Rodney |4 aut | |
| 700 | 1 | |a Munkhtsog, Bariusha |4 aut | |
| 700 | 1 | |a Murphy, William J. |4 aut | |
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10.1007/s12686-013-0096-1 doi (DE-627)SPR027439194 (SPR)s12686-013-0096-1-e DE-627 ger DE-627 rakwb eng Janecka, Jan E. verfasserin aut Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media Dordrecht 2013 Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. Microsatellites (dpeaa)DE-He213 Snow leopard (dpeaa)DE-He213 Noninvasive genetics (dpeaa)DE-He213 Individual identification (dpeaa)DE-He213 Jackson, Rodney aut Munkhtsog, Bariusha aut Murphy, William J. aut Enthalten in Conservation genetics resources Dordrecht : Springer Netherlands, 2009 6(2013), 2 vom: 06. Dez., Seite 369-373 (DE-627)606030891 (DE-600)2508018-0 1877-7260 nnns volume:6 year:2013 number:2 day:06 month:12 pages:369-373 https://dx.doi.org/10.1007/s12686-013-0096-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 2 06 12 369-373 |
| spelling |
10.1007/s12686-013-0096-1 doi (DE-627)SPR027439194 (SPR)s12686-013-0096-1-e DE-627 ger DE-627 rakwb eng Janecka, Jan E. verfasserin aut Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media Dordrecht 2013 Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. Microsatellites (dpeaa)DE-He213 Snow leopard (dpeaa)DE-He213 Noninvasive genetics (dpeaa)DE-He213 Individual identification (dpeaa)DE-He213 Jackson, Rodney aut Munkhtsog, Bariusha aut Murphy, William J. aut Enthalten in Conservation genetics resources Dordrecht : Springer Netherlands, 2009 6(2013), 2 vom: 06. Dez., Seite 369-373 (DE-627)606030891 (DE-600)2508018-0 1877-7260 nnns volume:6 year:2013 number:2 day:06 month:12 pages:369-373 https://dx.doi.org/10.1007/s12686-013-0096-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 2 06 12 369-373 |
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10.1007/s12686-013-0096-1 doi (DE-627)SPR027439194 (SPR)s12686-013-0096-1-e DE-627 ger DE-627 rakwb eng Janecka, Jan E. verfasserin aut Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media Dordrecht 2013 Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. Microsatellites (dpeaa)DE-He213 Snow leopard (dpeaa)DE-He213 Noninvasive genetics (dpeaa)DE-He213 Individual identification (dpeaa)DE-He213 Jackson, Rodney aut Munkhtsog, Bariusha aut Murphy, William J. aut Enthalten in Conservation genetics resources Dordrecht : Springer Netherlands, 2009 6(2013), 2 vom: 06. Dez., Seite 369-373 (DE-627)606030891 (DE-600)2508018-0 1877-7260 nnns volume:6 year:2013 number:2 day:06 month:12 pages:369-373 https://dx.doi.org/10.1007/s12686-013-0096-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 2 06 12 369-373 |
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10.1007/s12686-013-0096-1 doi (DE-627)SPR027439194 (SPR)s12686-013-0096-1-e DE-627 ger DE-627 rakwb eng Janecka, Jan E. verfasserin aut Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media Dordrecht 2013 Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. Microsatellites (dpeaa)DE-He213 Snow leopard (dpeaa)DE-He213 Noninvasive genetics (dpeaa)DE-He213 Individual identification (dpeaa)DE-He213 Jackson, Rodney aut Munkhtsog, Bariusha aut Murphy, William J. aut Enthalten in Conservation genetics resources Dordrecht : Springer Netherlands, 2009 6(2013), 2 vom: 06. Dez., Seite 369-373 (DE-627)606030891 (DE-600)2508018-0 1877-7260 nnns volume:6 year:2013 number:2 day:06 month:12 pages:369-373 https://dx.doi.org/10.1007/s12686-013-0096-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 2 06 12 369-373 |
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10.1007/s12686-013-0096-1 doi (DE-627)SPR027439194 (SPR)s12686-013-0096-1-e DE-627 ger DE-627 rakwb eng Janecka, Jan E. verfasserin aut Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media Dordrecht 2013 Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. Microsatellites (dpeaa)DE-He213 Snow leopard (dpeaa)DE-He213 Noninvasive genetics (dpeaa)DE-He213 Individual identification (dpeaa)DE-He213 Jackson, Rodney aut Munkhtsog, Bariusha aut Murphy, William J. aut Enthalten in Conservation genetics resources Dordrecht : Springer Netherlands, 2009 6(2013), 2 vom: 06. Dez., Seite 369-373 (DE-627)606030891 (DE-600)2508018-0 1877-7260 nnns volume:6 year:2013 number:2 day:06 month:12 pages:369-373 https://dx.doi.org/10.1007/s12686-013-0096-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 6 2013 2 06 12 369-373 |
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Enthalten in Conservation genetics resources 6(2013), 2 vom: 06. Dez., Seite 369-373 volume:6 year:2013 number:2 day:06 month:12 pages:369-373 |
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Janecka, Jan E. @@aut@@ Jackson, Rodney @@aut@@ Munkhtsog, Bariusha @@aut@@ Murphy, William J. @@aut@@ |
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Janecka, Jan E. |
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Janecka, Jan E. misc Microsatellites misc Snow leopard misc Noninvasive genetics misc Individual identification Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
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Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples Microsatellites (dpeaa)DE-He213 Snow leopard (dpeaa)DE-He213 Noninvasive genetics (dpeaa)DE-He213 Individual identification (dpeaa)DE-He213 |
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Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
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Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
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Janecka, Jan E. |
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characterization of 9 microsatellites and primers in snow leopards and a species-specific pcr assay for identifying noninvasive samples |
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Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
| abstract |
Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. © Springer Science+Business Media Dordrecht 2013 |
| abstractGer |
Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. © Springer Science+Business Media Dordrecht 2013 |
| abstract_unstemmed |
Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation ($ H_{e} $ = 0.504, $ A_{n} $ = 3.6) for individual identification and population structure analysis. We designed a species-specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. © Springer Science+Business Media Dordrecht 2013 |
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Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
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https://dx.doi.org/10.1007/s12686-013-0096-1 |
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Jackson, Rodney Munkhtsog, Bariusha Murphy, William J. |
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2024-07-04T01:52:37.878Z |
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