Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns

Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employe...
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Autor*in:	Ojurongbe, Olusola [verfasserIn] Ouf, Eman Abou Van Tong, Hoang Toan, Nguyen L Song, Le H Luz, Paola R Messias-Reason, Iara JT Nurjadi, Dennis Zanger, Philipp Kun, Jürgen FJ Kremsner, Peter G Velavan, Thirumalaisamy P

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	FRET Ficolin-2 Genotypes Haplotypes Distribution

Anmerkung:	© Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: BMC medical genetics - London : BioMed Central, 2000, 13(2012), 1 vom: 17. Mai
Übergeordnetes Werk:	volume:13 ; year:2012 ; number:1 ; day:17 ; month:05

Links:	Volltext

DOI / URN:	10.1186/1471-2350-13-37

Katalog-ID:	SPR027490483

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520			\|a Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping.
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650		4	\|a Haplotypes \|7 (dpeaa)DE-He213
650		4	\|a Distribution \|7 (dpeaa)DE-He213
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700	1		\|a Velavan, Thirumalaisamy P \|4 aut
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author_variant	o o oo e a o ea eao t h v th thv n l t nl nlt l h s lh lhs p r l pr prl i j m r ijm ijmr d n dn p z pz j f k jf jfk p g k pg pgk t p v tp tpv
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allfields	10.1186/1471-2350-13-37 doi (DE-627)SPR027490483 (SPR)1471-2350-13-37-e DE-627 ger DE-627 rakwb eng Ojurongbe, Olusola verfasserin aut Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. FRET (dpeaa)DE-He213 Ficolin-2 (dpeaa)DE-He213 Genotypes (dpeaa)DE-He213 Haplotypes (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Ouf, Eman Abou aut Van Tong, Hoang aut Toan, Nguyen L aut Song, Le H aut Luz, Paola R aut Messias-Reason, Iara JT aut Nurjadi, Dennis aut Zanger, Philipp aut Kun, Jürgen FJ aut Kremsner, Peter G aut Velavan, Thirumalaisamy P aut Enthalten in BMC medical genetics London : BioMed Central, 2000 13(2012), 1 vom: 17. Mai (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:13 year:2012 number:1 day:17 month:05 https://dx.doi.org/10.1186/1471-2350-13-37 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1 17 05
spelling	10.1186/1471-2350-13-37 doi (DE-627)SPR027490483 (SPR)1471-2350-13-37-e DE-627 ger DE-627 rakwb eng Ojurongbe, Olusola verfasserin aut Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. FRET (dpeaa)DE-He213 Ficolin-2 (dpeaa)DE-He213 Genotypes (dpeaa)DE-He213 Haplotypes (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Ouf, Eman Abou aut Van Tong, Hoang aut Toan, Nguyen L aut Song, Le H aut Luz, Paola R aut Messias-Reason, Iara JT aut Nurjadi, Dennis aut Zanger, Philipp aut Kun, Jürgen FJ aut Kremsner, Peter G aut Velavan, Thirumalaisamy P aut Enthalten in BMC medical genetics London : BioMed Central, 2000 13(2012), 1 vom: 17. Mai (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:13 year:2012 number:1 day:17 month:05 https://dx.doi.org/10.1186/1471-2350-13-37 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1 17 05
allfields_unstemmed	10.1186/1471-2350-13-37 doi (DE-627)SPR027490483 (SPR)1471-2350-13-37-e DE-627 ger DE-627 rakwb eng Ojurongbe, Olusola verfasserin aut Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. FRET (dpeaa)DE-He213 Ficolin-2 (dpeaa)DE-He213 Genotypes (dpeaa)DE-He213 Haplotypes (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Ouf, Eman Abou aut Van Tong, Hoang aut Toan, Nguyen L aut Song, Le H aut Luz, Paola R aut Messias-Reason, Iara JT aut Nurjadi, Dennis aut Zanger, Philipp aut Kun, Jürgen FJ aut Kremsner, Peter G aut Velavan, Thirumalaisamy P aut Enthalten in BMC medical genetics London : BioMed Central, 2000 13(2012), 1 vom: 17. Mai (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:13 year:2012 number:1 day:17 month:05 https://dx.doi.org/10.1186/1471-2350-13-37 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1 17 05
allfieldsGer	10.1186/1471-2350-13-37 doi (DE-627)SPR027490483 (SPR)1471-2350-13-37-e DE-627 ger DE-627 rakwb eng Ojurongbe, Olusola verfasserin aut Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. FRET (dpeaa)DE-He213 Ficolin-2 (dpeaa)DE-He213 Genotypes (dpeaa)DE-He213 Haplotypes (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Ouf, Eman Abou aut Van Tong, Hoang aut Toan, Nguyen L aut Song, Le H aut Luz, Paola R aut Messias-Reason, Iara JT aut Nurjadi, Dennis aut Zanger, Philipp aut Kun, Jürgen FJ aut Kremsner, Peter G aut Velavan, Thirumalaisamy P aut Enthalten in BMC medical genetics London : BioMed Central, 2000 13(2012), 1 vom: 17. Mai (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:13 year:2012 number:1 day:17 month:05 https://dx.doi.org/10.1186/1471-2350-13-37 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1 17 05
allfieldsSound	10.1186/1471-2350-13-37 doi (DE-627)SPR027490483 (SPR)1471-2350-13-37-e DE-627 ger DE-627 rakwb eng Ojurongbe, Olusola verfasserin aut Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. FRET (dpeaa)DE-He213 Ficolin-2 (dpeaa)DE-He213 Genotypes (dpeaa)DE-He213 Haplotypes (dpeaa)DE-He213 Distribution (dpeaa)DE-He213 Ouf, Eman Abou aut Van Tong, Hoang aut Toan, Nguyen L aut Song, Le H aut Luz, Paola R aut Messias-Reason, Iara JT aut Nurjadi, Dennis aut Zanger, Philipp aut Kun, Jürgen FJ aut Kremsner, Peter G aut Velavan, Thirumalaisamy P aut Enthalten in BMC medical genetics London : BioMed Central, 2000 13(2012), 1 vom: 17. Mai (DE-627)326643788 (DE-600)2041359-2 1471-2350 nnns volume:13 year:2012 number:1 day:17 month:05 https://dx.doi.org/10.1186/1471-2350-13-37 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2012 1 17 05
language	English
source	Enthalten in BMC medical genetics 13(2012), 1 vom: 17. Mai volume:13 year:2012 number:1 day:17 month:05
sourceStr	Enthalten in BMC medical genetics 13(2012), 1 vom: 17. Mai volume:13 year:2012 number:1 day:17 month:05
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	FRET Ficolin-2 Genotypes Haplotypes Distribution
isfreeaccess_bool	true
container_title	BMC medical genetics
authorswithroles_txt_mv	Ojurongbe, Olusola @@aut@@ Ouf, Eman Abou @@aut@@ Van Tong, Hoang @@aut@@ Toan, Nguyen L @@aut@@ Song, Le H @@aut@@ Luz, Paola R @@aut@@ Messias-Reason, Iara JT @@aut@@ Nurjadi, Dennis @@aut@@ Zanger, Philipp @@aut@@ Kun, Jürgen FJ @@aut@@ Kremsner, Peter G @@aut@@ Velavan, Thirumalaisamy P @@aut@@
publishDateDaySort_date	2012-05-17T00:00:00Z
hierarchy_top_id	326643788
id	SPR027490483
language_de	englisch
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. 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author	Ojurongbe, Olusola
spellingShingle	Ojurongbe, Olusola misc FRET misc Ficolin-2 misc Genotypes misc Haplotypes misc Distribution Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns
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topic_title	Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns FRET (dpeaa)DE-He213 Ficolin-2 (dpeaa)DE-He213 Genotypes (dpeaa)DE-He213 Haplotypes (dpeaa)DE-He213 Distribution (dpeaa)DE-He213
topic	misc FRET misc Ficolin-2 misc Genotypes misc Haplotypes misc Distribution
topic_unstemmed	misc FRET misc Ficolin-2 misc Genotypes misc Haplotypes misc Distribution
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title	Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns
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title_full	Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns
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author_browse	Ojurongbe, Olusola Ouf, Eman Abou Van Tong, Hoang Toan, Nguyen L Song, Le H Luz, Paola R Messias-Reason, Iara JT Nurjadi, Dennis Zanger, Philipp Kun, Jürgen FJ Kremsner, Peter G Velavan, Thirumalaisamy P
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author-letter	Ojurongbe, Olusola
doi_str_mv	10.1186/1471-2350-13-37
title_sort	reliable and rapid characterization of functional fcn2 gene variants reveals diverse geographical patterns
title_auth	Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns
abstract	Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Background Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. Methods We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). Results We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). Conclusions The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping. © Ojurongbe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns
url	https://dx.doi.org/10.1186/1471-2350-13-37
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author2	Ouf, Eman Abou Van Tong, Hoang Toan, Nguyen L Song, Le H Luz, Paola R Messias-Reason, Iara JT Nurjadi, Dennis Zanger, Philipp Kun, Jürgen FJ Kremsner, Peter G Velavan, Thirumalaisamy P
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Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns

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